Potato virus H

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6Z8L3|I6Z8L3_9VIRU 26K protein OS=Potato virus H OX=1046402 GN=TGB1 PE=4 SV=1
MM1 pKa = 7.86DD2 pKa = 4.2VLVDD6 pKa = 4.43LLNKK10 pKa = 10.25YY11 pKa = 10.52SFTRR15 pKa = 11.84IHH17 pKa = 6.8SKK19 pKa = 10.29LSSPIVVHH27 pKa = 6.1CVPGAGKK34 pKa = 8.68TSLIRR39 pKa = 11.84EE40 pKa = 4.62LIKK43 pKa = 10.57LDD45 pKa = 3.65SRR47 pKa = 11.84FVAYY51 pKa = 8.36TAGVEE56 pKa = 4.26DD57 pKa = 4.79EE58 pKa = 4.39PHH60 pKa = 7.11LSGRR64 pKa = 11.84WIRR67 pKa = 11.84KK68 pKa = 8.62FEE70 pKa = 4.06GVVDD74 pKa = 3.76EE75 pKa = 4.85GKK77 pKa = 10.54FVILDD82 pKa = 3.89EE83 pKa = 4.35YY84 pKa = 10.8TLLEE88 pKa = 4.34SLPDD92 pKa = 3.33NLFAVFGDD100 pKa = 4.57PIQSNTKK107 pKa = 7.79VVRR110 pKa = 11.84SADD113 pKa = 3.64YY114 pKa = 9.14TCNRR118 pKa = 11.84SKK120 pKa = 11.11RR121 pKa = 11.84FGRR124 pKa = 11.84STALFLRR131 pKa = 11.84EE132 pKa = 4.02LGFDD136 pKa = 3.72VVAEE140 pKa = 4.4ADD142 pKa = 4.03DD143 pKa = 4.07EE144 pKa = 4.75VAVANIYY151 pKa = 10.25QVDD154 pKa = 3.73PVEE157 pKa = 3.87QVVYY161 pKa = 9.74FEE163 pKa = 4.65QEE165 pKa = 4.0VGCLLRR171 pKa = 11.84AHH173 pKa = 6.57HH174 pKa = 6.42VEE176 pKa = 4.19CKK178 pKa = 10.13HH179 pKa = 4.34YY180 pKa = 9.97TEE182 pKa = 5.33IVGQTFEE189 pKa = 4.36KK190 pKa = 9.56VTFVSSEE197 pKa = 4.3SNLSSNRR204 pKa = 11.84VAAYY208 pKa = 10.0QCMTRR213 pKa = 11.84HH214 pKa = 6.02RR215 pKa = 11.84SKK217 pKa = 11.22LLILTPDD224 pKa = 3.21ATFTAAA230 pKa = 4.8

Molecular weight: 25.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6YW94|I6YW94_9VIRU Capsid protein OS=Potato virus H OX=1046402 GN=CP PE=3 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84LSSKK6 pKa = 8.49VLRR9 pKa = 11.84KK10 pKa = 10.16VLVGVFKK17 pKa = 10.57RR18 pKa = 11.84HH19 pKa = 5.67YY20 pKa = 9.22GLGPRR25 pKa = 11.84RR26 pKa = 11.84EE27 pKa = 4.12YY28 pKa = 11.5DD29 pKa = 4.04DD30 pKa = 3.59IASVIISKK38 pKa = 10.64LEE40 pKa = 3.87PPEE43 pKa = 4.52VGLSTYY49 pKa = 10.74AKK51 pKa = 9.8RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84AKK56 pKa = 10.57KK57 pKa = 9.97LGRR60 pKa = 11.84CPRR63 pKa = 11.84CYY65 pKa = 9.88RR66 pKa = 11.84VIGLSQKK73 pKa = 10.51CDD75 pKa = 3.09GFKK78 pKa = 10.42CVPGISYY85 pKa = 9.83NRR87 pKa = 11.84KK88 pKa = 7.83VEE90 pKa = 3.86QNIRR94 pKa = 11.84YY95 pKa = 9.73GDD97 pKa = 3.32

Molecular weight: 11.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2743

67

1949

457.2

51.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.984 ± 0.441

2.771 ± 0.445

4.666 ± 0.44

7.437 ± 1.09

4.557 ± 0.348

5.724 ± 0.301

2.224 ± 0.243

4.521 ± 0.951

6.38 ± 0.505

9.807 ± 0.782

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.361

3.5 ± 0.461

3.901 ± 0.967

2.661 ± 0.18

6.161 ± 0.594

7.838 ± 0.56

4.812 ± 0.469

8.057 ± 0.722

0.948 ± 0.136

3.609 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski