Modestobacter roseus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Geodermatophilales; Geodermatophilaceae; Modestobacter

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4272 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562ITE4|A0A562ITE4_9ACTN N-acetyltransferase domain-containing protein OS=Modestobacter roseus OX=1181884 GN=JD78_02629 PE=4 SV=1
MM1 pKa = 6.77STSSRR6 pKa = 11.84RR7 pKa = 11.84LAGVVLAAGLGTATLTGCAVTFVEE31 pKa = 5.48GTATPLSGLRR41 pKa = 11.84GPDD44 pKa = 3.17EE45 pKa = 4.13PAEE48 pKa = 4.13FPVVGAVDD56 pKa = 3.74SDD58 pKa = 3.44VDD60 pKa = 3.92QIARR64 pKa = 11.84NALADD69 pKa = 3.99LNLYY73 pKa = 9.19WGEE76 pKa = 4.05QFGPVYY82 pKa = 10.06QQEE85 pKa = 4.34FPPLTGGYY93 pKa = 10.2YY94 pKa = 10.45SVDD97 pKa = 3.61PDD99 pKa = 5.47DD100 pKa = 5.23IDD102 pKa = 3.7PVAYY106 pKa = 9.31PGGEE110 pKa = 4.1IGCGEE115 pKa = 4.3PPEE118 pKa = 5.03AVTDD122 pKa = 3.55NAFYY126 pKa = 10.68CGPSQEE132 pKa = 4.6YY133 pKa = 9.88PNGDD137 pKa = 3.89AIQYY141 pKa = 10.25DD142 pKa = 4.0RR143 pKa = 11.84AFLDD147 pKa = 3.45EE148 pKa = 4.53LAFGTGGSEE157 pKa = 4.33GYY159 pKa = 10.84GRR161 pKa = 11.84FIPALVMAHH170 pKa = 6.2EE171 pKa = 5.39FGHH174 pKa = 6.49AVQGRR179 pKa = 11.84VGYY182 pKa = 8.95PFQASIAIEE191 pKa = 4.06TQADD195 pKa = 3.98CFAGAWTRR203 pKa = 11.84WVADD207 pKa = 3.4GEE209 pKa = 4.39APHH212 pKa = 6.6NSIRR216 pKa = 11.84PAEE219 pKa = 4.34LDD221 pKa = 3.5DD222 pKa = 3.8VLRR225 pKa = 11.84GYY227 pKa = 11.18LLLRR231 pKa = 11.84DD232 pKa = 3.91PVGTGLNAGEE242 pKa = 4.05AHH244 pKa = 6.71GSYY247 pKa = 10.43FDD249 pKa = 3.88RR250 pKa = 11.84VSAFQQGFDD259 pKa = 4.79DD260 pKa = 5.89GPTACRR266 pKa = 11.84DD267 pKa = 3.56DD268 pKa = 4.04FGGDD272 pKa = 2.95RR273 pKa = 11.84PYY275 pKa = 10.6TQGAFRR281 pKa = 11.84DD282 pKa = 4.1DD283 pKa = 4.63DD284 pKa = 4.22DD285 pKa = 6.0ALTGGNAPYY294 pKa = 11.01ALTVDD299 pKa = 3.4QFLPEE304 pKa = 4.07GLGEE308 pKa = 4.26FWQQVLADD316 pKa = 4.16RR317 pKa = 11.84GRR319 pKa = 11.84TFRR322 pKa = 11.84PPRR325 pKa = 11.84LEE327 pKa = 4.28PFDD330 pKa = 4.76GRR332 pKa = 11.84APDD335 pKa = 4.32CADD338 pKa = 3.31EE339 pKa = 4.57GADD342 pKa = 3.74VDD344 pKa = 4.86LVYY347 pKa = 10.8CAGEE351 pKa = 3.96DD352 pKa = 3.28TVAFDD357 pKa = 4.48EE358 pKa = 5.16ADD360 pKa = 3.46LTEE363 pKa = 4.55PLYY366 pKa = 10.15QAEE369 pKa = 4.31RR370 pKa = 11.84GGDD373 pKa = 3.76YY374 pKa = 11.24AVLTAVAIPYY384 pKa = 9.75GLAGRR389 pKa = 11.84AQLGLSTDD397 pKa = 3.27GQEE400 pKa = 4.32ALRR403 pKa = 11.84SAVCLSGAFTASVLNGEE420 pKa = 4.44STVLSISPGDD430 pKa = 3.44VDD432 pKa = 3.81EE433 pKa = 5.0SVSFLLEE440 pKa = 3.98YY441 pKa = 10.0ATDD444 pKa = 4.22PDD446 pKa = 4.12VLPDD450 pKa = 3.58AEE452 pKa = 4.37LTGFQLVDD460 pKa = 3.08VFRR463 pKa = 11.84TGVFDD468 pKa = 5.51GPAACDD474 pKa = 3.57LDD476 pKa = 4.06SS477 pKa = 4.21

Molecular weight: 50.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562IVM7|A0A562IVM7_9ACTN RND superfamily putative drug exporter OS=Modestobacter roseus OX=1181884 GN=JD78_03452 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SKK15 pKa = 8.58THH17 pKa = 5.46GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAGRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.38GRR40 pKa = 11.84DD41 pKa = 3.05KK42 pKa = 11.36LSAA45 pKa = 3.77

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4272

0

4272

1363073

39

2612

319.1

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.698 ± 0.06

0.639 ± 0.01

6.273 ± 0.028

5.39 ± 0.036

2.516 ± 0.021

9.798 ± 0.04

1.983 ± 0.018

2.645 ± 0.025

1.254 ± 0.023

10.665 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.535 ± 0.014

1.402 ± 0.017

6.243 ± 0.032

2.821 ± 0.021

7.97 ± 0.037

4.767 ± 0.026

6.247 ± 0.034

9.989 ± 0.036

1.497 ± 0.016

1.669 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski