Mycobacterium phage Gaia

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Gaiavirus; Mycobacterium virus Gaia

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 179 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068F4Q7|A0A068F4Q7_9CAUD Uncharacterized protein OS=Mycobacterium phage Gaia OX=1486472 GN=142 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 5.82NDD4 pKa = 3.81NNEE7 pKa = 4.12CDD9 pKa = 4.61CIGEE13 pKa = 3.98GDD15 pKa = 3.62IILRR19 pKa = 11.84EE20 pKa = 3.97LRR22 pKa = 11.84IVEE25 pKa = 4.13ILSAEE30 pKa = 4.12DD31 pKa = 3.59GEE33 pKa = 4.67IYY35 pKa = 10.52KK36 pKa = 9.86IDD38 pKa = 4.0LSHH41 pKa = 7.67DD42 pKa = 3.64GGGEE46 pKa = 4.11LPTDD50 pKa = 4.71GYY52 pKa = 11.71LLLAEE57 pKa = 4.37WAKK60 pKa = 11.06SLATAPIIADD70 pKa = 3.6LVMGYY75 pKa = 8.75VQSLEE80 pKa = 4.07DD81 pKa = 4.65DD82 pKa = 3.85EE83 pKa = 5.23

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068F3H6|A0A068F3H6_9CAUD Uncharacterized protein OS=Mycobacterium phage Gaia OX=1486472 GN=74 PE=4 SV=1
MM1 pKa = 7.49PQFIDD6 pKa = 3.57DD7 pKa = 4.55PRR9 pKa = 11.84WSTEE13 pKa = 3.77NVSKK17 pKa = 10.77IMQHH21 pKa = 5.93NGIHH25 pKa = 6.1SPADD29 pKa = 3.39LAKK32 pKa = 10.56AIPISRR38 pKa = 11.84ATLYY42 pKa = 10.75RR43 pKa = 11.84AFGTNWSGRR52 pKa = 11.84ATEE55 pKa = 4.36RR56 pKa = 11.84LVEE59 pKa = 4.79AIAVRR64 pKa = 11.84FRR66 pKa = 11.84VPSYY70 pKa = 11.2LLVKK74 pKa = 10.09EE75 pKa = 4.97SPCSAPQPTKK85 pKa = 10.43RR86 pKa = 11.84PEE88 pKa = 3.77SGKK91 pKa = 10.17RR92 pKa = 11.84NRR94 pKa = 11.84SGSRR98 pKa = 11.84GDD100 pKa = 3.2RR101 pKa = 3.48

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

179

0

179

27531

18

1287

153.8

17.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.647 ± 0.327

1.54 ± 0.146

6.694 ± 0.152

6.513 ± 0.207

3.018 ± 0.117

8.423 ± 0.368

2.317 ± 0.142

4.878 ± 0.161

4.09 ± 0.201

7.987 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.1

3.574 ± 0.123

5.103 ± 0.198

2.993 ± 0.142

6.898 ± 0.288

6.338 ± 0.203

5.434 ± 0.191

6.774 ± 0.152

2.31 ± 0.103

3.033 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski