Amino acid dipepetide frequency for Thermoanaerobacterium sp. RBIITD

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.076AlaAla: 4.076 ± 0.09	0.637AlaCys: 0.637 ± 0.028	2.984AlaAsp: 2.984 ± 0.059	3.236AlaGlu: 3.236 ± 0.061	2.558AlaPhe: 2.558 ± 0.058	3.943AlaGly: 3.943 ± 0.08	0.885AlaHis: 0.885 ± 0.033	5.975AlaIle: 5.975 ± 0.094	4.99AlaLys: 4.99 ± 0.078	5.85AlaLeu: 5.85 ± 0.088	1.625AlaMet: 1.625 ± 0.045	2.736AlaAsn: 2.736 ± 0.057	1.42AlaPro: 1.42 ± 0.037	1.495AlaGln: 1.495 ± 0.045	1.971AlaArg: 1.971 ± 0.051	3.285AlaSer: 3.285 ± 0.06	2.724AlaThr: 2.724 ± 0.061	4.263AlaVal: 4.263 ± 0.074	0.406AlaTrp: 0.406 ± 0.024	2.374AlaTyr: 2.374 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.526CysAla: 0.526 ± 0.022	0.128CysCys: 0.128 ± 0.012	0.667CysAsp: 0.667 ± 0.032	0.57CysGlu: 0.57 ± 0.025	0.411CysPhe: 0.411 ± 0.021	0.973CysGly: 0.973 ± 0.039	0.22CysHis: 0.22 ± 0.015	0.948CysIle: 0.948 ± 0.035	0.744CysLys: 0.744 ± 0.028	0.696CysLeu: 0.696 ± 0.024	0.241CysMet: 0.241 ± 0.016	0.553CysAsn: 0.553 ± 0.025	0.511CysPro: 0.511 ± 0.026	0.213CysGln: 0.213 ± 0.016	0.359CysArg: 0.359 ± 0.019	0.551CysSer: 0.551 ± 0.026	0.514CysThr: 0.514 ± 0.023	0.512CysVal: 0.512 ± 0.023	0.071CysTrp: 0.071 ± 0.009	0.361CysTyr: 0.361 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	3.437AspAla: 3.437 ± 0.06	0.521AspCys: 0.521 ± 0.024	3.818AspAsp: 3.818 ± 0.076	4.735AspGlu: 4.735 ± 0.084	2.821AspPhe: 2.821 ± 0.061	3.838AspGly: 3.838 ± 0.075	0.694AspHis: 0.694 ± 0.029	7.187AspIle: 7.187 ± 0.096	5.532AspLys: 5.532 ± 0.085	5.035AspLeu: 5.035 ± 0.08	1.649AspMet: 1.649 ± 0.043	3.673AspAsn: 3.673 ± 0.066	1.743AspPro: 1.743 ± 0.049	0.857AspGln: 0.857 ± 0.036	2.087AspArg: 2.087 ± 0.057	2.926AspSer: 2.926 ± 0.06	2.723AspThr: 2.723 ± 0.057	4.609AspVal: 4.609 ± 0.071	0.523AspTrp: 0.523 ± 0.025	2.732AspTyr: 2.732 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	3.701GluAla: 3.701 ± 0.072	0.566GluCys: 0.566 ± 0.024	3.622GluAsp: 3.622 ± 0.081	5.034GluGlu: 5.034 ± 0.101	2.57GluPhe: 2.57 ± 0.057	3.564GluGly: 3.564 ± 0.071	0.881GluHis: 0.881 ± 0.031	6.917GluIle: 6.917 ± 0.107	6.828GluLys: 6.828 ± 0.094	6.147GluLeu: 6.147 ± 0.102	1.887GluMet: 1.887 ± 0.037	4.547GluAsn: 4.547 ± 0.078	1.484GluPro: 1.484 ± 0.037	1.618GluGln: 1.618 ± 0.047	2.882GluArg: 2.882 ± 0.064	2.935GluSer: 2.935 ± 0.064	2.623GluThr: 2.623 ± 0.053	4.098GluVal: 4.098 ± 0.076	0.503GluTrp: 0.503 ± 0.025	3.068GluTyr: 3.068 ± 0.066	0.0GluXaa: 0.0 ± 0.0
Phe	2.486PheAla: 2.486 ± 0.053	0.418PheCys: 0.418 ± 0.021	2.884PheAsp: 2.884 ± 0.054	2.572PheGlu: 2.572 ± 0.057	1.859PhePhe: 1.859 ± 0.059	2.865PheGly: 2.865 ± 0.067	0.504PheHis: 0.504 ± 0.021	4.513PheIle: 4.513 ± 0.086	3.615PheLys: 3.615 ± 0.063	3.809PheLeu: 3.809 ± 0.081	1.129PheMet: 1.129 ± 0.04	2.62PheAsn: 2.62 ± 0.064	1.255PhePro: 1.255 ± 0.042	0.843PheGln: 0.843 ± 0.035	1.306PheArg: 1.306 ± 0.034	2.772PheSer: 2.772 ± 0.062	2.236PheThr: 2.236 ± 0.044	2.73PheVal: 2.73 ± 0.057	0.313PheTrp: 0.313 ± 0.022	1.88PheTyr: 1.88 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	3.879GlyAla: 3.879 ± 0.076	0.792GlyCys: 0.792 ± 0.032	3.483GlyAsp: 3.483 ± 0.061	3.651GlyGlu: 3.651 ± 0.064	3.05GlyPhe: 3.05 ± 0.063	4.415GlyGly: 4.415 ± 0.101	1.12GlyHis: 1.12 ± 0.036	7.165GlyIle: 7.165 ± 0.097	5.548GlyLys: 5.548 ± 0.077	5.756GlyLeu: 5.756 ± 0.09	1.72GlyMet: 1.72 ± 0.047	3.445GlyAsn: 3.445 ± 0.075	1.597GlyPro: 1.597 ± 0.053	1.481GlyGln: 1.481 ± 0.042	2.426GlyArg: 2.426 ± 0.05	3.614GlySer: 3.614 ± 0.071	3.545GlyThr: 3.545 ± 0.071	4.227GlyVal: 4.227 ± 0.068	0.563GlyTrp: 0.563 ± 0.028	3.266GlyTyr: 3.266 ± 0.07	0.0GlyXaa: 0.0 ± 0.0
His	0.748HisAla: 0.748 ± 0.031	0.193HisCys: 0.193 ± 0.016	0.825HisAsp: 0.825 ± 0.03	0.871HisGlu: 0.871 ± 0.026	0.69HisPhe: 0.69 ± 0.029	1.041HisGly: 1.041 ± 0.039	0.277HisHis: 0.277 ± 0.02	1.426HisIle: 1.426 ± 0.04	0.987HisLys: 0.987 ± 0.035	1.245HisLeu: 1.245 ± 0.036	0.378HisMet: 0.378 ± 0.02	0.75HisAsn: 0.75 ± 0.028	0.678HisPro: 0.678 ± 0.03	0.318HisGln: 0.318 ± 0.021	0.541HisArg: 0.541 ± 0.024	0.76HisSer: 0.76 ± 0.029	0.715HisThr: 0.715 ± 0.035	0.888HisVal: 0.888 ± 0.028	0.134HisTrp: 0.134 ± 0.012	0.658HisTyr: 0.658 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.187IleAla: 6.187 ± 0.102	0.984IleCys: 0.984 ± 0.037	7.137IleAsp: 7.137 ± 0.093	6.829IleGlu: 6.829 ± 0.1	4.186IlePhe: 4.186 ± 0.085	6.514IleGly: 6.514 ± 0.096	1.222IleHis: 1.222 ± 0.037	9.91IleIle: 9.91 ± 0.175	9.442IleLys: 9.442 ± 0.113	9.059IleLeu: 9.059 ± 0.142	2.529IleMet: 2.529 ± 0.056	6.652IleAsn: 6.652 ± 0.101	3.863IlePro: 3.863 ± 0.067	1.982IleGln: 1.982 ± 0.048	3.363IleArg: 3.363 ± 0.071	6.678IleSer: 6.678 ± 0.103	4.849IleThr: 4.849 ± 0.082	6.445IleVal: 6.445 ± 0.106	0.583IleTrp: 0.583 ± 0.029	3.953IleTyr: 3.953 ± 0.071	0.0IleXaa: 0.0 ± 0.0
Lys	4.99LysAla: 4.99 ± 0.074	0.786LysCys: 0.786 ± 0.034	6.057LysAsp: 6.057 ± 0.1	7.413LysGlu: 7.413 ± 0.123	2.993LysPhe: 2.993 ± 0.065	4.952LysGly: 4.952 ± 0.08	1.077LysHis: 1.077 ± 0.038	8.667LysIle: 8.667 ± 0.116	8.001LysLys: 8.001 ± 0.115	7.498LysLeu: 7.498 ± 0.1	2.461LysMet: 2.461 ± 0.053	6.461LysAsn: 6.461 ± 0.089	2.583LysPro: 2.583 ± 0.055	2.153LysGln: 2.153 ± 0.056	3.484LysArg: 3.484 ± 0.07	4.968LysSer: 4.968 ± 0.081	4.021LysThr: 4.021 ± 0.064	5.332LysVal: 5.332 ± 0.075	0.649LysTrp: 0.649 ± 0.028	4.608LysTyr: 4.608 ± 0.076	0.0LysXaa: 0.0 ± 0.0
Leu	4.743LeuAla: 4.743 ± 0.079	0.846LeuCys: 0.846 ± 0.031	5.019LeuAsp: 5.019 ± 0.078	5.134LeuGlu: 5.134 ± 0.083	3.804LeuPhe: 3.804 ± 0.083	5.388LeuGly: 5.388 ± 0.087	1.214LeuHis: 1.214 ± 0.038	8.679LeuIle: 8.679 ± 0.121	9.44LeuLys: 9.44 ± 0.112	8.15LeuLeu: 8.15 ± 0.11	2.315LeuMet: 2.315 ± 0.054	6.412LeuAsn: 6.412 ± 0.087	3.18LeuPro: 3.18 ± 0.056	2.011LeuGln: 2.011 ± 0.053	3.339LeuArg: 3.339 ± 0.067	6.511LeuSer: 6.511 ± 0.087	4.444LeuThr: 4.444 ± 0.071	4.772LeuVal: 4.772 ± 0.074	0.685LeuTrp: 0.685 ± 0.032	3.684LeuTyr: 3.684 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	1.793MetAla: 1.793 ± 0.05	0.215MetCys: 0.215 ± 0.018	1.466MetAsp: 1.466 ± 0.042	1.617MetGlu: 1.617 ± 0.045	0.991MetPhe: 0.991 ± 0.036	1.749MetGly: 1.749 ± 0.051	0.365MetHis: 0.365 ± 0.018	2.39MetIle: 2.39 ± 0.057	2.348MetLys: 2.348 ± 0.047	2.513MetLeu: 2.513 ± 0.057	0.78MetMet: 0.78 ± 0.031	1.586MetAsn: 1.586 ± 0.044	1.144MetPro: 1.144 ± 0.04	0.681MetGln: 0.681 ± 0.028	1.065MetArg: 1.065 ± 0.038	1.613MetSer: 1.613 ± 0.041	1.343MetThr: 1.343 ± 0.043	1.674MetVal: 1.674 ± 0.046	0.193MetTrp: 0.193 ± 0.014	0.911MetTyr: 0.911 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	3.239AsnAla: 3.239 ± 0.064	0.577AsnCys: 0.577 ± 0.026	3.781AsnAsp: 3.781 ± 0.077	3.996AsnGlu: 3.996 ± 0.064	2.605AsnPhe: 2.605 ± 0.055	4.051AsnGly: 4.051 ± 0.091	0.781AsnHis: 0.781 ± 0.028	7.264AsnIle: 7.264 ± 0.106	5.429AsnLys: 5.429 ± 0.091	5.312AsnLeu: 5.312 ± 0.077	1.683AsnMet: 1.683 ± 0.051	4.211AsnAsn: 4.211 ± 0.085	2.354AsnPro: 2.354 ± 0.059	1.345AsnGln: 1.345 ± 0.037	1.972AsnArg: 1.972 ± 0.041	3.45AsnSer: 3.45 ± 0.071	3.042AsnThr: 3.042 ± 0.067	4.059AsnVal: 4.059 ± 0.072	0.511AsnTrp: 0.511 ± 0.025	2.808AsnTyr: 2.808 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	1.722ProAla: 1.722 ± 0.047	0.313ProCys: 0.313 ± 0.02	2.334ProAsp: 2.334 ± 0.053	2.397ProGlu: 2.397 ± 0.054	1.533ProPhe: 1.533 ± 0.039	2.128ProGly: 2.128 ± 0.056	0.585ProHis: 0.585 ± 0.027	3.034ProIle: 3.034 ± 0.06	2.192ProLys: 2.192 ± 0.049	2.791ProLeu: 2.791 ± 0.056	0.79ProMet: 0.79 ± 0.032	1.644ProAsn: 1.644 ± 0.055	0.905ProPro: 0.905 ± 0.031	1.007ProGln: 1.007 ± 0.035	0.872ProArg: 0.872 ± 0.033	1.814ProSer: 1.814 ± 0.047	1.645ProThr: 1.645 ± 0.048	2.508ProVal: 2.508 ± 0.05	0.302ProTrp: 0.302 ± 0.018	1.634ProTyr: 1.634 ± 0.049	0.0ProXaa: 0.0 ± 0.0
Gln	1.388GlnAla: 1.388 ± 0.047	0.212GlnCys: 0.212 ± 0.017	1.296GlnAsp: 1.296 ± 0.04	1.526GlnGlu: 1.526 ± 0.043	0.948GlnPhe: 0.948 ± 0.03	1.324GlnGly: 1.324 ± 0.046	0.339GlnHis: 0.339 ± 0.023	2.208GlnIle: 2.208 ± 0.053	2.244GlnLys: 2.244 ± 0.049	2.021GlnLeu: 2.021 ± 0.046	0.656GlnMet: 0.656 ± 0.027	1.662GlnAsn: 1.662 ± 0.046	0.626GlnPro: 0.626 ± 0.028	0.787GlnGln: 0.787 ± 0.047	0.98GlnArg: 0.98 ± 0.041	1.229GlnSer: 1.229 ± 0.04	1.044GlnThr: 1.044 ± 0.039	1.374GlnVal: 1.374 ± 0.042	0.216GlnTrp: 0.216 ± 0.016	1.13GlnTyr: 1.13 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	1.923ArgAla: 1.923 ± 0.042	0.435ArgCys: 0.435 ± 0.024	2.086ArgAsp: 2.086 ± 0.055	2.694ArgGlu: 2.694 ± 0.059	1.523ArgPhe: 1.523 ± 0.041	2.175ArgGly: 2.175 ± 0.061	0.606ArgHis: 0.606 ± 0.024	3.501ArgIle: 3.501 ± 0.061	3.162ArgLys: 3.162 ± 0.067	3.39ArgLeu: 3.39 ± 0.072	1.031ArgMet: 1.031 ± 0.034	2.116ArgAsn: 2.116 ± 0.053	1.134ArgPro: 1.134 ± 0.034	1.025ArgGln: 1.025 ± 0.032	1.588ArgArg: 1.588 ± 0.05	1.687ArgSer: 1.687 ± 0.047	1.61ArgThr: 1.61 ± 0.043	2.141ArgVal: 2.141 ± 0.048	0.313ArgTrp: 0.313 ± 0.018	1.693ArgTyr: 1.693 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	3.037SerAla: 3.037 ± 0.07	0.509SerCys: 0.509 ± 0.027	3.477SerAsp: 3.477 ± 0.067	3.353SerGlu: 3.353 ± 0.057	2.808SerPhe: 2.808 ± 0.054	4.293SerGly: 4.293 ± 0.078	0.864SerHis: 0.864 ± 0.033	6.247SerIle: 6.247 ± 0.096	4.912SerLys: 4.912 ± 0.072	5.475SerLeu: 5.475 ± 0.089	1.552SerMet: 1.552 ± 0.043	3.501SerAsn: 3.501 ± 0.075	1.771SerPro: 1.771 ± 0.045	1.527SerGln: 1.527 ± 0.045	2.075SerArg: 2.075 ± 0.044	3.603SerSer: 3.603 ± 0.08	2.98SerThr: 2.98 ± 0.059	3.529SerVal: 3.529 ± 0.063	0.445SerTrp: 0.445 ± 0.024	2.54SerTyr: 2.54 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	2.926ThrAla: 2.926 ± 0.071	0.423ThrCys: 0.423 ± 0.021	2.679ThrAsp: 2.679 ± 0.058	2.61ThrGlu: 2.61 ± 0.053	2.141ThrPhe: 2.141 ± 0.053	3.9ThrGly: 3.9 ± 0.08	0.798ThrHis: 0.798 ± 0.031	4.913ThrIle: 4.913 ± 0.077	3.677ThrLys: 3.677 ± 0.076	4.415ThrLeu: 4.415 ± 0.076	1.121ThrMet: 1.121 ± 0.035	2.654ThrAsn: 2.654 ± 0.059	1.917ThrPro: 1.917 ± 0.052	1.165ThrGln: 1.165 ± 0.043	1.516ThrArg: 1.516 ± 0.043	2.857ThrSer: 2.857 ± 0.067	2.579ThrThr: 2.579 ± 0.072	3.55ThrVal: 3.55 ± 0.071	0.413ThrTrp: 0.413 ± 0.027	2.12ThrTyr: 2.12 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	3.859ValAla: 3.859 ± 0.085	0.67ValCys: 0.67 ± 0.03	4.073ValAsp: 4.073 ± 0.067	4.096ValGlu: 4.096 ± 0.083	2.717ValPhe: 2.717 ± 0.057	3.988ValGly: 3.988 ± 0.074	0.871ValHis: 0.871 ± 0.033	6.275ValIle: 6.275 ± 0.1	5.661ValLys: 5.661 ± 0.082	5.882ValLeu: 5.882 ± 0.081	1.641ValMet: 1.641 ± 0.04	3.754ValAsn: 3.754 ± 0.068	2.303ValPro: 2.303 ± 0.054	1.38ValGln: 1.38 ± 0.041	2.093ValArg: 2.093 ± 0.051	4.118ValSer: 4.118 ± 0.084	3.325ValThr: 3.325 ± 0.073	4.374ValVal: 4.374 ± 0.086	0.431ValTrp: 0.431 ± 0.025	2.686ValTyr: 2.686 ± 0.059	0.0ValXaa: 0.0 ± 0.0
Trp	0.463TrpAla: 0.463 ± 0.025	0.097TrpCys: 0.097 ± 0.01	0.489TrpAsp: 0.489 ± 0.023	0.418TrpGlu: 0.418 ± 0.023	0.326TrpPhe: 0.326 ± 0.021	0.552TrpGly: 0.552 ± 0.027	0.154TrpHis: 0.154 ± 0.012	0.68TrpIle: 0.68 ± 0.029	0.537TrpLys: 0.537 ± 0.025	0.682TrpLeu: 0.682 ± 0.03	0.216TrpMet: 0.216 ± 0.014	0.547TrpAsn: 0.547 ± 0.025	0.234TrpPro: 0.234 ± 0.017	0.284TrpGln: 0.284 ± 0.021	0.319TrpArg: 0.319 ± 0.02	0.426TrpSer: 0.426 ± 0.026	0.384TrpThr: 0.384 ± 0.021	0.441TrpVal: 0.441 ± 0.025	0.133TrpTrp: 0.133 ± 0.013	0.364TrpTyr: 0.364 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.31TyrAla: 2.31 ± 0.046	0.449TyrCys: 0.449 ± 0.024	2.961TyrAsp: 2.961 ± 0.061	2.725TyrGlu: 2.725 ± 0.058	2.077TyrPhe: 2.077 ± 0.056	3.088TyrGly: 3.088 ± 0.055	0.665TyrHis: 0.665 ± 0.028	4.468TyrIle: 4.468 ± 0.084	3.865TyrLys: 3.865 ± 0.065	4.047TyrLeu: 4.047 ± 0.076	1.032TyrMet: 1.032 ± 0.033	2.947TyrAsn: 2.947 ± 0.064	1.448TyrPro: 1.448 ± 0.044	1.04TyrGln: 1.04 ± 0.037	1.595TyrArg: 1.595 ± 0.044	2.699TyrSer: 2.699 ± 0.052	2.029TyrThr: 2.029 ± 0.047	2.669TyrVal: 2.669 ± 0.06	0.356TyrTrp: 0.356 ± 0.021	2.043TyrTyr: 2.043 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2986 proteins (911818 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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