Agaricus bisporus var. burnettii (strain JB137-S8 / ATCC MYA-4627 / FGSC 10392) (White button mushroom)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Agaricaceae; Agaricus; Agaricus bisporus; Agaricus bisporus var. burnettii

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K5VTA9|K5VTA9_AGABU Uncharacterized protein OS=Agaricus bisporus var. burnettii (strain JB137-S8 / ATCC MYA-4627 / FGSC 10392) OX=597362 GN=AGABI1DRAFT_93062 PE=4 SV=1
MM1 pKa = 7.18VKK3 pKa = 8.68ITALFVLSALSCLVSAAPVLEE24 pKa = 3.92KK25 pKa = 10.59RR26 pKa = 11.84IAQVTSDD33 pKa = 4.87SKK35 pKa = 11.43AAWEE39 pKa = 4.21QACVAAGGGAQCNKK53 pKa = 9.92IGINAASTLLAAADD67 pKa = 3.54ACAQQDD73 pKa = 3.77AADD76 pKa = 4.65DD77 pKa = 4.47MIDD80 pKa = 4.64LAKK83 pKa = 10.78QLDD86 pKa = 4.24NDD88 pKa = 4.01PEE90 pKa = 4.4MIRR93 pKa = 11.84LAQLFVQQPRR103 pKa = 11.84NAPDD107 pKa = 3.46SLQVPYY113 pKa = 10.37CQQAPKK119 pKa = 10.39NAEE122 pKa = 3.8LDD124 pKa = 4.01GLFHH128 pKa = 6.75CQFEE132 pKa = 4.78GSKK135 pKa = 10.95FPDD138 pKa = 3.93FSGDD142 pKa = 3.21QTGNVPLGLNQVNPPGSCPANPTGPIPDD170 pKa = 3.69GVQLNTLVDD179 pKa = 4.27DD180 pKa = 5.16PGLEE184 pKa = 4.06NAAGGASSGGASSGGAAPSDD204 pKa = 3.52NSSADD209 pKa = 3.75DD210 pKa = 3.95EE211 pKa = 4.84ASSDD215 pKa = 3.64INNGANGGAAGGAEE229 pKa = 4.64DD230 pKa = 4.49NDD232 pKa = 3.94ASGGAIGNNSDD243 pKa = 3.58TGATGAGGDD252 pKa = 3.57FVLQNGQDD260 pKa = 3.49AQSQNSQFADD270 pKa = 3.27ITADD274 pKa = 3.66SPCTDD279 pKa = 3.82GEE281 pKa = 4.37NACVGSDD288 pKa = 3.52FAQCVGGKK296 pKa = 9.48FVTTSCGGNLICAALPLVNAPGTSVTCTTAADD328 pKa = 3.78RR329 pKa = 11.84DD330 pKa = 3.74ARR332 pKa = 11.84IAATGAA338 pKa = 3.25

Molecular weight: 33.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K5XHN1|K5XHN1_AGABU Uncharacterized protein (Fragment) OS=Agaricus bisporus var. burnettii (strain JB137-S8 / ATCC MYA-4627 / FGSC 10392) OX=597362 GN=AGABI1DRAFT_95986 PE=4 SV=1
MM1 pKa = 7.68ASQLRR6 pKa = 11.84THH8 pKa = 7.37APHH11 pKa = 6.99AWDD14 pKa = 3.65TTSTRR19 pKa = 11.84IGASPTCLRR28 pKa = 11.84ASTSTTTPSGSHH40 pKa = 5.69TPSPHH45 pKa = 5.78SLGPISGPKK54 pKa = 9.33DD55 pKa = 3.28ASIACSFCGRR65 pKa = 11.84VGHH68 pKa = 5.72MPRR71 pKa = 11.84RR72 pKa = 11.84CRR74 pKa = 11.84KK75 pKa = 8.74QKK77 pKa = 9.9HH78 pKa = 4.24ARR80 pKa = 11.84KK81 pKa = 9.1RR82 pKa = 11.84AQEE85 pKa = 3.88RR86 pKa = 11.84SRR88 pKa = 11.84LPGTSKK94 pKa = 11.35SNVTGPLGHH103 pKa = 7.35LSGIGAALPAVKK115 pKa = 9.42TPPTPPGDD123 pKa = 3.35VSTGSGSPGSDD134 pKa = 2.44IPLRR138 pKa = 11.84ASALGAYY145 pKa = 9.23SSPSSSPSSSSSPSSPLQLDD165 pKa = 3.98ADD167 pKa = 4.44FFWLADD173 pKa = 3.65TGATSHH179 pKa = 5.51MTPHH183 pKa = 6.4RR184 pKa = 11.84HH185 pKa = 4.39WFKK188 pKa = 10.55SYY190 pKa = 9.98SPHH193 pKa = 7.58RR194 pKa = 11.84IPIRR198 pKa = 11.84LADD201 pKa = 3.57NSTVYY206 pKa = 10.56SAGVGSS212 pKa = 3.81

Molecular weight: 22.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11204

7

11211

4612704

43

4900

411.4

45.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.698 ± 0.018

1.238 ± 0.009

5.606 ± 0.017

6.004 ± 0.023

3.911 ± 0.017

6.317 ± 0.022

2.594 ± 0.011

5.292 ± 0.015

4.938 ± 0.023

9.151 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.008

3.845 ± 0.012

6.353 ± 0.027

3.79 ± 0.016

6.115 ± 0.018

8.73 ± 0.03

6.0 ± 0.014

6.171 ± 0.015

1.428 ± 0.01

2.733 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski