Mycobacterium phage Serenity

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M5M0Z7|A0A0M5M0Z7_9CAUD Uncharacterized protein OS=Mycobacterium phage Serenity OX=1701853 GN=SEA_SERENITY_96 PE=4 SV=1
MM1 pKa = 7.41TPDD4 pKa = 2.96EE5 pKa = 4.76MYY7 pKa = 10.45TFAVYY12 pKa = 10.88YY13 pKa = 8.27EE14 pKa = 4.46FRR16 pKa = 11.84PSPEE20 pKa = 4.34SEE22 pKa = 3.82WQEE25 pKa = 3.9VVAVQQTLAEE35 pKa = 4.2AQAVFNGISPIAYY48 pKa = 8.43TNEE51 pKa = 3.56NLRR54 pKa = 11.84NLMLAYY60 pKa = 9.19TPKK63 pKa = 10.04IDD65 pKa = 3.16WQQYY69 pKa = 6.61SS70 pKa = 3.2

Molecular weight: 8.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4R0P6|A0A0M4R0P6_9CAUD Head-to-tail connector protein OS=Mycobacterium phage Serenity OX=1701853 GN=SEA_SERENITY_18 PE=4 SV=1
MM1 pKa = 7.25SWAGSGRR8 pKa = 11.84RR9 pKa = 11.84QEE11 pKa = 5.38LPEE14 pKa = 4.38DD15 pKa = 3.29WEE17 pKa = 4.35LNYY20 pKa = 10.43RR21 pKa = 11.84LPVLSRR27 pKa = 11.84DD28 pKa = 3.13RR29 pKa = 11.84WICQINGPGCVRR41 pKa = 11.84AATDD45 pKa = 2.9VDD47 pKa = 4.26HH48 pKa = 7.46IKK50 pKa = 10.61RR51 pKa = 11.84GNDD54 pKa = 3.12HH55 pKa = 6.26SHH57 pKa = 6.89SNLQSLCWVCHH68 pKa = 4.91GKK70 pKa = 10.31KK71 pKa = 10.28SSAEE75 pKa = 3.71GVARR79 pKa = 11.84RR80 pKa = 11.84RR81 pKa = 11.84EE82 pKa = 3.69LRR84 pKa = 11.84ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.79RR89 pKa = 11.84PPEE92 pKa = 3.6RR93 pKa = 11.84HH94 pKa = 5.7PGRR97 pKa = 11.84RR98 pKa = 3.38

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

16103

25

838

166.0

18.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.83 ± 0.378

0.869 ± 0.105

6.322 ± 0.222

7.079 ± 0.329

3.521 ± 0.19

8.21 ± 0.432

2.155 ± 0.185

5.018 ± 0.16

4.813 ± 0.217

8.023 ± 0.302

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.614 ± 0.165

3.242 ± 0.169

5.31 ± 0.24

3.596 ± 0.219

6.583 ± 0.413

5.043 ± 0.21

5.844 ± 0.239

7.061 ± 0.196

1.969 ± 0.145

2.9 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski