Neospora caninum (strain Liverpool)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Sarcocystidae; Neospora; Neospora caninum

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6933 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0VPG0|F0VPG0_NEOCL Uncharacterized protein OS=Neospora caninum (strain Liverpool) OX=572307 GN=BN1204_060310 PE=4 SV=1
MM1 pKa = 7.76GEE3 pKa = 4.01AQTDD7 pKa = 3.5YY8 pKa = 10.71SLQYY12 pKa = 10.13RR13 pKa = 11.84GVPYY17 pKa = 10.54VPASLPPEE25 pKa = 4.52LPSSLQYY32 pKa = 11.34DD33 pKa = 4.12SLTLSDD39 pKa = 6.03FSQQPHH45 pKa = 6.16GFGGSAGEE53 pKa = 3.96NSGLNTPQNIDD64 pKa = 3.2LGDD67 pKa = 4.23RR68 pKa = 11.84EE69 pKa = 5.65DD70 pKa = 3.83EE71 pKa = 4.36PQPLRR76 pKa = 4.14

Molecular weight: 8.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0VQU0|F0VQU0_NEOCL Putative endonuclease/exonuclease/phosphatase domain-containing protein OS=Neospora caninum (strain Liverpool) OX=572307 GN=NCLIV_065140 PE=4 SV=1
SS1 pKa = 7.2LDD3 pKa = 2.85AWYY6 pKa = 8.88PPARR10 pKa = 11.84RR11 pKa = 11.84VSTKK15 pKa = 9.59PCRR18 pKa = 11.84ARR20 pKa = 11.84PGDD23 pKa = 4.01PTWLGAEE30 pKa = 4.41SPQLLASRR38 pKa = 11.84PPKK41 pKa = 9.72RR42 pKa = 11.84AEE44 pKa = 3.76NEE46 pKa = 3.96KK47 pKa = 10.25TGDD50 pKa = 3.65EE51 pKa = 4.29TGGTQLTKK59 pKa = 10.77AKK61 pKa = 9.77KK62 pKa = 9.03SKK64 pKa = 9.77KK65 pKa = 10.04AKK67 pKa = 9.25STTQMRR73 pKa = 11.84KK74 pKa = 8.84KK75 pKa = 10.29RR76 pKa = 11.84KK77 pKa = 7.02TGVRR81 pKa = 11.84AEE83 pKa = 4.41GTRR86 pKa = 11.84SLALSRR92 pKa = 11.84RR93 pKa = 11.84EE94 pKa = 3.79RR95 pKa = 11.84RR96 pKa = 11.84NTRR99 pKa = 11.84SVQRR103 pKa = 11.84GRR105 pKa = 11.84RR106 pKa = 11.84GRR108 pKa = 11.84MRR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84MRR114 pKa = 11.84RR115 pKa = 11.84SRR117 pKa = 11.84VRR119 pKa = 11.84RR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84MRR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84MRR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84MRR135 pKa = 11.84RR136 pKa = 11.84RR137 pKa = 11.84RR138 pKa = 11.84MRR140 pKa = 11.84RR141 pKa = 11.84RR142 pKa = 11.84MRR144 pKa = 11.84RR145 pKa = 11.84RR146 pKa = 11.84MRR148 pKa = 11.84RR149 pKa = 11.84SRR151 pKa = 11.84VRR153 pKa = 11.84RR154 pKa = 11.84RR155 pKa = 11.84RR156 pKa = 11.84MRR158 pKa = 11.84RR159 pKa = 11.84RR160 pKa = 11.84RR161 pKa = 11.84RR162 pKa = 11.84MRR164 pKa = 11.84RR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84MRR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 11.84RR172 pKa = 11.84MRR174 pKa = 11.84RR175 pKa = 11.84RR176 pKa = 11.84RR177 pKa = 11.84MRR179 pKa = 11.84RR180 pKa = 11.84RR181 pKa = 11.84RR182 pKa = 11.84MRR184 pKa = 11.84RR185 pKa = 11.84SRR187 pKa = 11.84VRR189 pKa = 11.84RR190 pKa = 11.84RR191 pKa = 11.84RR192 pKa = 11.84IRR194 pKa = 11.84RR195 pKa = 11.84RR196 pKa = 11.84RR197 pKa = 11.84RR198 pKa = 11.84MRR200 pKa = 11.84RR201 pKa = 11.84RR202 pKa = 11.84RR203 pKa = 11.84MRR205 pKa = 11.84RR206 pKa = 11.84RR207 pKa = 11.84RR208 pKa = 11.84MRR210 pKa = 11.84RR211 pKa = 11.84RR212 pKa = 11.84RR213 pKa = 11.84RR214 pKa = 11.84RR215 pKa = 11.84RR216 pKa = 11.84RR217 pKa = 11.84QRR219 pKa = 11.84DD220 pKa = 2.89KK221 pKa = 11.66GLL223 pKa = 3.5

Molecular weight: 29.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6930

3

6933

5937456

23

16227

856.4

92.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.074 ± 0.038

1.889 ± 0.013

4.942 ± 0.014

7.678 ± 0.033

3.286 ± 0.018

8.016 ± 0.035

2.145 ± 0.01

2.324 ± 0.02

3.93 ± 0.025

8.874 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.342 ± 0.011

2.231 ± 0.014

6.76 ± 0.028

3.898 ± 0.017

8.226 ± 0.036

10.276 ± 0.041

4.898 ± 0.018

5.733 ± 0.021

1.014 ± 0.007

1.463 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski