Amino acid dipepetide frequency for Microbacterium sp. T32

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.639AlaAla: 21.639 ± 0.204	0.647AlaCys: 0.647 ± 0.028	8.786AlaAsp: 8.786 ± 0.107	8.345AlaGlu: 8.345 ± 0.124	4.024AlaPhe: 4.024 ± 0.071	12.018AlaGly: 12.018 ± 0.126	2.645AlaHis: 2.645 ± 0.053	5.728AlaIle: 5.728 ± 0.088	2.475AlaLys: 2.475 ± 0.07	14.668AlaLeu: 14.668 ± 0.168	2.633AlaMet: 2.633 ± 0.047	2.197AlaAsn: 2.197 ± 0.054	7.031AlaPro: 7.031 ± 0.13	3.643AlaGln: 3.643 ± 0.06	10.107AlaArg: 10.107 ± 0.116	7.563AlaSer: 7.563 ± 0.092	7.678AlaThr: 7.678 ± 0.104	12.502AlaVal: 12.502 ± 0.133	2.061AlaTrp: 2.061 ± 0.056	2.446AlaTyr: 2.446 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.63CysAla: 0.63 ± 0.025	0.047CysCys: 0.047 ± 0.007	0.279CysAsp: 0.279 ± 0.016	0.234CysGlu: 0.234 ± 0.014	0.169CysPhe: 0.169 ± 0.013	0.52CysGly: 0.52 ± 0.025	0.11CysHis: 0.11 ± 0.012	0.171CysIle: 0.171 ± 0.014	0.053CysLys: 0.053 ± 0.008	0.408CysLeu: 0.408 ± 0.02	0.063CysMet: 0.063 ± 0.008	0.089CysAsn: 0.089 ± 0.009	0.254CysPro: 0.254 ± 0.018	0.127CysGln: 0.127 ± 0.012	0.304CysArg: 0.304 ± 0.019	0.27CysSer: 0.27 ± 0.018	0.278CysThr: 0.278 ± 0.018	0.402CysVal: 0.402 ± 0.021	0.052CysTrp: 0.052 ± 0.007	0.091CysTyr: 0.091 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	10.283AspAla: 10.283 ± 0.123	0.218AspCys: 0.218 ± 0.015	4.82AspAsp: 4.82 ± 0.079	4.128AspGlu: 4.128 ± 0.073	1.722AspPhe: 1.722 ± 0.048	6.277AspGly: 6.277 ± 0.09	1.198AspHis: 1.198 ± 0.038	2.386AspIle: 2.386 ± 0.051	0.975AspLys: 0.975 ± 0.036	6.724AspLeu: 6.724 ± 0.076	0.823AspMet: 0.823 ± 0.028	0.938AspAsn: 0.938 ± 0.033	4.37AspPro: 4.37 ± 0.077	1.49AspGln: 1.49 ± 0.043	4.814AspArg: 4.814 ± 0.082	2.307AspSer: 2.307 ± 0.057	3.149AspThr: 3.149 ± 0.051	5.855AspVal: 5.855 ± 0.075	0.949AspTrp: 0.949 ± 0.03	1.2AspTyr: 1.2 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.019GluAla: 7.019 ± 0.101	0.208GluCys: 0.208 ± 0.014	2.609GluAsp: 2.609 ± 0.058	2.793GluGlu: 2.793 ± 0.07	1.642GluPhe: 1.642 ± 0.046	4.061GluGly: 4.061 ± 0.068	1.268GluHis: 1.268 ± 0.036	2.885GluIle: 2.885 ± 0.051	1.352GluLys: 1.352 ± 0.046	5.825GluLeu: 5.825 ± 0.082	0.87GluMet: 0.87 ± 0.03	1.256GluAsn: 1.256 ± 0.036	2.908GluPro: 2.908 ± 0.067	1.88GluGln: 1.88 ± 0.044	5.364GluArg: 5.364 ± 0.088	2.588GluSer: 2.588 ± 0.055	3.091GluThr: 3.091 ± 0.061	4.598GluVal: 4.598 ± 0.082	0.952GluTrp: 0.952 ± 0.032	1.159GluTyr: 1.159 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.558PheAla: 4.558 ± 0.071	0.15PheCys: 0.15 ± 0.012	2.454PheAsp: 2.454 ± 0.055	1.538PheGlu: 1.538 ± 0.046	1.095PhePhe: 1.095 ± 0.035	3.32PheGly: 3.32 ± 0.059	0.569PheHis: 0.569 ± 0.025	0.997PheIle: 0.997 ± 0.036	0.408PheLys: 0.408 ± 0.023	3.023PheLeu: 3.023 ± 0.059	0.372PheMet: 0.372 ± 0.02	0.562PheAsn: 0.562 ± 0.024	1.455PhePro: 1.455 ± 0.036	0.742PheGln: 0.742 ± 0.025	1.925PheArg: 1.925 ± 0.042	1.797PheSer: 1.797 ± 0.046	2.119PheThr: 2.119 ± 0.044	3.052PheVal: 3.052 ± 0.065	0.507PheTrp: 0.507 ± 0.024	0.658PheTyr: 0.658 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	10.776GlyAla: 10.776 ± 0.12	0.516GlyCys: 0.516 ± 0.023	5.318GlyAsp: 5.318 ± 0.078	4.752GlyGlu: 4.752 ± 0.075	3.261GlyPhe: 3.261 ± 0.058	7.73GlyGly: 7.73 ± 0.131	1.74GlyHis: 1.74 ± 0.043	4.908GlyIle: 4.908 ± 0.075	2.035GlyLys: 2.035 ± 0.05	8.739GlyLeu: 8.739 ± 0.098	1.805GlyMet: 1.805 ± 0.047	1.679GlyAsn: 1.679 ± 0.054	4.039GlyPro: 4.039 ± 0.062	2.353GlyGln: 2.353 ± 0.05	6.6GlyArg: 6.6 ± 0.085	5.318GlySer: 5.318 ± 0.074	5.447GlyThr: 5.447 ± 0.092	8.247GlyVal: 8.247 ± 0.099	1.633GlyTrp: 1.633 ± 0.044	2.133GlyTyr: 2.133 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.37HisAla: 2.37 ± 0.054	0.097HisCys: 0.097 ± 0.011	1.329HisAsp: 1.329 ± 0.04	1.073HisGlu: 1.073 ± 0.03	0.53HisPhe: 0.53 ± 0.021	1.899HisGly: 1.899 ± 0.05	0.495HisHis: 0.495 ± 0.021	0.683HisIle: 0.683 ± 0.027	0.251HisLys: 0.251 ± 0.017	2.024HisLeu: 2.024 ± 0.05	0.297HisMet: 0.297 ± 0.018	0.314HisAsn: 0.314 ± 0.02	1.513HisPro: 1.513 ± 0.036	0.422HisGln: 0.422 ± 0.017	1.621HisArg: 1.621 ± 0.045	0.87HisSer: 0.87 ± 0.033	0.964HisThr: 0.964 ± 0.036	1.808HisVal: 1.808 ± 0.043	0.291HisTrp: 0.291 ± 0.018	0.377HisTyr: 0.377 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.864IleAla: 6.864 ± 0.083	0.205IleCys: 0.205 ± 0.013	3.472IleAsp: 3.472 ± 0.052	2.657IleGlu: 2.657 ± 0.061	1.162IlePhe: 1.162 ± 0.041	4.397IleGly: 4.397 ± 0.072	0.699IleHis: 0.699 ± 0.027	1.54IleIle: 1.54 ± 0.056	0.668IleLys: 0.668 ± 0.029	3.618IleLeu: 3.618 ± 0.062	0.493IleMet: 0.493 ± 0.021	0.821IleAsn: 0.821 ± 0.028	2.459IlePro: 2.459 ± 0.049	0.976IleGln: 0.976 ± 0.029	2.775IleArg: 2.775 ± 0.051	2.074IleSer: 2.074 ± 0.052	2.567IleThr: 2.567 ± 0.055	4.653IleVal: 4.653 ± 0.078	0.46IleTrp: 0.46 ± 0.022	0.703IleTyr: 0.703 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	2.233LysAla: 2.233 ± 0.059	0.061LysCys: 0.061 ± 0.009	1.121LysAsp: 1.121 ± 0.036	0.88LysGlu: 0.88 ± 0.036	0.47LysPhe: 0.47 ± 0.025	1.463LysGly: 1.463 ± 0.05	0.355LysHis: 0.355 ± 0.021	0.89LysIle: 0.89 ± 0.031	0.737LysLys: 0.737 ± 0.033	1.722LysLeu: 1.722 ± 0.045	0.335LysMet: 0.335 ± 0.018	0.533LysAsn: 0.533 ± 0.028	1.05LysPro: 1.05 ± 0.038	0.601LysGln: 0.601 ± 0.027	1.366LysArg: 1.366 ± 0.044	0.971LysSer: 0.971 ± 0.034	1.238LysThr: 1.238 ± 0.04	1.596LysVal: 1.596 ± 0.041	0.235LysTrp: 0.235 ± 0.016	0.439LysTyr: 0.439 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.867LeuAla: 14.867 ± 0.151	0.43LeuCys: 0.43 ± 0.021	6.852LeuAsp: 6.852 ± 0.085	5.013LeuGlu: 5.013 ± 0.081	2.868LeuPhe: 2.868 ± 0.066	9.066LeuGly: 9.066 ± 0.127	1.854LeuHis: 1.854 ± 0.047	4.104LeuIle: 4.104 ± 0.082	1.534LeuLys: 1.534 ± 0.048	10.07LeuLeu: 10.07 ± 0.148	1.421LeuMet: 1.421 ± 0.041	1.658LeuAsn: 1.658 ± 0.04	5.782LeuPro: 5.782 ± 0.081	2.541LeuGln: 2.541 ± 0.056	7.974LeuArg: 7.974 ± 0.106	5.934LeuSer: 5.934 ± 0.079	6.394LeuThr: 6.394 ± 0.069	9.374LeuVal: 9.374 ± 0.112	1.299LeuTrp: 1.299 ± 0.041	1.599LeuTyr: 1.599 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	1.901MetAla: 1.901 ± 0.05	0.1MetCys: 0.1 ± 0.011	0.835MetAsp: 0.835 ± 0.033	0.546MetGlu: 0.546 ± 0.025	0.465MetPhe: 0.465 ± 0.025	1.205MetGly: 1.205 ± 0.032	0.262MetHis: 0.262 ± 0.015	0.807MetIle: 0.807 ± 0.026	0.41MetLys: 0.41 ± 0.021	1.642MetLeu: 1.642 ± 0.044	0.27MetMet: 0.27 ± 0.019	0.448MetAsn: 0.448 ± 0.021	1.11MetPro: 1.11 ± 0.036	0.484MetGln: 0.484 ± 0.024	1.235MetArg: 1.235 ± 0.038	1.444MetSer: 1.444 ± 0.034	1.649MetThr: 1.649 ± 0.044	1.185MetVal: 1.185 ± 0.035	0.193MetTrp: 0.193 ± 0.015	0.265MetTyr: 0.265 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.497AsnAla: 2.497 ± 0.052	0.104AsnCys: 0.104 ± 0.011	1.189AsnAsp: 1.189 ± 0.036	0.848AsnGlu: 0.848 ± 0.027	0.594AsnPhe: 0.594 ± 0.025	1.872AsnGly: 1.872 ± 0.045	0.329AsnHis: 0.329 ± 0.019	0.75AsnIle: 0.75 ± 0.027	0.354AsnLys: 0.354 ± 0.022	1.778AsnLeu: 1.778 ± 0.046	0.279AsnMet: 0.279 ± 0.018	0.41AsnAsn: 0.41 ± 0.021	1.532AsnPro: 1.532 ± 0.044	0.462AsnGln: 0.462 ± 0.021	1.206AsnArg: 1.206 ± 0.038	0.888AsnSer: 0.888 ± 0.034	1.101AsnThr: 1.101 ± 0.038	1.692AsnVal: 1.692 ± 0.046	0.26AsnTrp: 0.26 ± 0.018	0.44AsnTyr: 0.44 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.772ProAla: 7.772 ± 0.108	0.178ProCys: 0.178 ± 0.014	4.253ProAsp: 4.253 ± 0.069	3.717ProGlu: 3.717 ± 0.059	1.864ProPhe: 1.864 ± 0.039	5.221ProGly: 5.221 ± 0.089	1.113ProHis: 1.113 ± 0.031	2.011ProIle: 2.011 ± 0.051	0.948ProLys: 0.948 ± 0.037	5.093ProLeu: 5.093 ± 0.074	0.787ProMet: 0.787 ± 0.032	0.971ProAsn: 0.971 ± 0.029	2.438ProPro: 2.438 ± 0.066	1.388ProGln: 1.388 ± 0.04	3.855ProArg: 3.855 ± 0.069	3.235ProSer: 3.235 ± 0.058	3.709ProThr: 3.709 ± 0.081	5.158ProVal: 5.158 ± 0.08	0.951ProTrp: 0.951 ± 0.032	1.009ProTyr: 1.009 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.267GlnAla: 3.267 ± 0.06	0.1GlnCys: 0.1 ± 0.01	1.206GlnAsp: 1.206 ± 0.039	1.127GlnGlu: 1.127 ± 0.034	0.764GlnPhe: 0.764 ± 0.026	1.952GlnGly: 1.952 ± 0.053	0.541GlnHis: 0.541 ± 0.022	1.276GlnIle: 1.276 ± 0.04	0.67GlnLys: 0.67 ± 0.028	2.881GlnLeu: 2.881 ± 0.061	0.463GlnMet: 0.463 ± 0.026	0.657GlnAsn: 0.657 ± 0.029	1.484GlnPro: 1.484 ± 0.045	1.067GlnGln: 1.067 ± 0.035	2.38GlnArg: 2.38 ± 0.052	1.373GlnSer: 1.373 ± 0.039	1.569GlnThr: 1.569 ± 0.043	2.112GlnVal: 2.112 ± 0.047	0.501GlnTrp: 0.501 ± 0.023	0.616GlnTyr: 0.616 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	10.102ArgAla: 10.102 ± 0.135	0.29ArgCys: 0.29 ± 0.017	4.612ArgAsp: 4.612 ± 0.09	4.555ArgGlu: 4.555 ± 0.07	2.513ArgPhe: 2.513 ± 0.057	5.971ArgGly: 5.971 ± 0.084	1.567ArgHis: 1.567 ± 0.04	3.53ArgIle: 3.53 ± 0.057	1.281ArgLys: 1.281 ± 0.045	7.61ArgLeu: 7.61 ± 0.108	1.61ArgMet: 1.61 ± 0.037	1.319ArgAsn: 1.319 ± 0.039	3.974ArgPro: 3.974 ± 0.066	1.887ArgGln: 1.887 ± 0.047	7.293ArgArg: 7.293 ± 0.134	4.111ArgSer: 4.111 ± 0.076	4.444ArgThr: 4.444 ± 0.065	6.72ArgVal: 6.72 ± 0.098	1.329ArgTrp: 1.329 ± 0.035	1.56ArgTyr: 1.56 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.218SerAla: 7.218 ± 0.091	0.26SerCys: 0.26 ± 0.017	3.267SerAsp: 3.267 ± 0.061	2.57SerGlu: 2.57 ± 0.056	1.919SerPhe: 1.919 ± 0.046	5.624SerGly: 5.624 ± 0.083	0.957SerHis: 0.957 ± 0.033	2.354SerIle: 2.354 ± 0.049	0.947SerLys: 0.947 ± 0.032	5.261SerLeu: 5.261 ± 0.08	1.083SerMet: 1.083 ± 0.033	0.994SerAsn: 0.994 ± 0.035	3.209SerPro: 3.209 ± 0.058	1.271SerGln: 1.271 ± 0.041	3.802SerArg: 3.802 ± 0.066	3.303SerSer: 3.303 ± 0.069	3.655SerThr: 3.655 ± 0.057	4.906SerVal: 4.906 ± 0.073	0.9SerTrp: 0.9 ± 0.029	1.109SerTyr: 1.109 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	7.927ThrAla: 7.927 ± 0.109	0.248ThrCys: 0.248 ± 0.015	3.776ThrAsp: 3.776 ± 0.064	2.847ThrGlu: 2.847 ± 0.059	1.982ThrPhe: 1.982 ± 0.051	5.811ThrGly: 5.811 ± 0.089	1.129ThrHis: 1.129 ± 0.037	2.735ThrIle: 2.735 ± 0.06	1.091ThrLys: 1.091 ± 0.033	6.215ThrLeu: 6.215 ± 0.076	0.907ThrMet: 0.907 ± 0.034	1.118ThrAsn: 1.118 ± 0.04	4.393ThrPro: 4.393 ± 0.08	1.395ThrGln: 1.395 ± 0.038	3.933ThrArg: 3.933 ± 0.05	3.368ThrSer: 3.368 ± 0.065	3.887ThrThr: 3.887 ± 0.081	6.178ThrVal: 6.178 ± 0.091	0.898ThrTrp: 0.898 ± 0.033	1.279ThrTyr: 1.279 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	12.729ValAla: 12.729 ± 0.124	0.501ValCys: 0.501 ± 0.024	6.22ValAsp: 6.22 ± 0.088	4.994ValGlu: 4.994 ± 0.077	2.997ValPhe: 2.997 ± 0.055	7.475ValGly: 7.475 ± 0.102	1.773ValHis: 1.773 ± 0.047	4.09ValIle: 4.09 ± 0.067	1.487ValLys: 1.487 ± 0.044	9.71ValLeu: 9.71 ± 0.121	1.332ValMet: 1.332 ± 0.032	1.713ValAsn: 1.713 ± 0.04	4.976ValPro: 4.976 ± 0.077	2.219ValGln: 2.219 ± 0.052	6.746ValArg: 6.746 ± 0.093	5.199ValSer: 5.199 ± 0.072	5.952ValThr: 5.952 ± 0.095	9.549ValVal: 9.549 ± 0.129	1.259ValTrp: 1.259 ± 0.041	1.538ValTyr: 1.538 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.692TrpAla: 1.692 ± 0.04	0.08TrpCys: 0.08 ± 0.009	0.838TrpAsp: 0.838 ± 0.029	0.66TrpGlu: 0.66 ± 0.026	0.574TrpPhe: 0.574 ± 0.025	1.252TrpGly: 1.252 ± 0.038	0.364TrpHis: 0.364 ± 0.02	0.755TrpIle: 0.755 ± 0.028	0.304TrpLys: 0.304 ± 0.016	1.591TrpLeu: 1.591 ± 0.043	0.355TrpMet: 0.355 ± 0.018	0.456TrpAsn: 0.456 ± 0.021	0.725TrpPro: 0.725 ± 0.027	0.509TrpGln: 0.509 ± 0.022	1.317TrpArg: 1.317 ± 0.041	0.95TrpSer: 0.95 ± 0.034	1.044TrpThr: 1.044 ± 0.037	1.236TrpVal: 1.236 ± 0.035	0.358TrpTrp: 0.358 ± 0.022	0.307TrpTyr: 0.307 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.494TyrAla: 2.494 ± 0.055	0.111TyrCys: 0.111 ± 0.01	1.389TyrAsp: 1.389 ± 0.044	1.05TyrGlu: 1.05 ± 0.035	0.687TyrPhe: 0.687 ± 0.024	1.895TyrGly: 1.895 ± 0.048	0.258TyrHis: 0.258 ± 0.015	0.661TyrIle: 0.661 ± 0.025	0.314TyrLys: 0.314 ± 0.018	2.033TyrLeu: 2.033 ± 0.052	0.259TyrMet: 0.259 ± 0.018	0.455TyrAsn: 0.455 ± 0.023	0.967TyrPro: 0.967 ± 0.032	0.549TyrGln: 0.549 ± 0.026	1.662TyrArg: 1.662 ± 0.038	1.046TyrSer: 1.046 ± 0.033	1.183TyrThr: 1.183 ± 0.035	1.635TyrVal: 1.635 ± 0.039	0.278TyrTrp: 0.278 ± 0.016	0.44TyrTyr: 0.44 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3267 proteins (1023341 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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