Tortoise microvirus 96

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W7P8|A0A4P8W7P8_9VIRU DNA pilot protein OS=Tortoise microvirus 96 OX=2583205 PE=4 SV=1
MM1 pKa = 8.18PINICPTARR10 pKa = 11.84EE11 pKa = 3.96PQVLDD16 pKa = 3.98FEE18 pKa = 5.3DD19 pKa = 4.39SVVEE23 pKa = 3.9PDD25 pKa = 3.14EE26 pKa = 4.69AMSVTDD32 pKa = 4.08IIRR35 pKa = 11.84RR36 pKa = 11.84HH37 pKa = 6.14LSGQPLSVPGDD48 pKa = 3.54YY49 pKa = 11.05DD50 pKa = 3.59GTDD53 pKa = 3.15VGDD56 pKa = 4.14NEE58 pKa = 5.93LDD60 pKa = 4.04DD61 pKa = 5.83SDD63 pKa = 6.53DD64 pKa = 3.79EE65 pKa = 4.35MFDD68 pKa = 5.08AISQRR73 pKa = 11.84IDD75 pKa = 3.43AMDD78 pKa = 3.57LTEE81 pKa = 4.71IDD83 pKa = 3.96EE84 pKa = 4.33MRR86 pKa = 11.84SMLNNRR92 pKa = 11.84AASIRR97 pKa = 11.84AQLKK101 pKa = 8.49AQEE104 pKa = 4.27DD105 pKa = 3.97AALKK109 pKa = 9.62QQIKK113 pKa = 11.2DD114 pKa = 3.61EE115 pKa = 4.25MEE117 pKa = 4.64KK118 pKa = 10.78DD119 pKa = 3.87SQSDD123 pKa = 3.59PLDD126 pKa = 3.84NSHH129 pKa = 7.38PAA131 pKa = 3.14

Molecular weight: 14.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FW21|A0A4V1FW21_9VIRU Uncharacterized protein OS=Tortoise microvirus 96 OX=2583205 PE=4 SV=1
MM1 pKa = 7.85LINNMLNFKK10 pKa = 8.04TFKK13 pKa = 9.95LWLIVVVVAAAAVAAFVVTAFRR35 pKa = 11.84VEE37 pKa = 3.89ASVCNKK43 pKa = 9.55QLIIMNCLSPIRR55 pKa = 11.84IKK57 pKa = 10.87NPKK60 pKa = 7.11PTYY63 pKa = 8.33YY64 pKa = 10.39SPRR67 pKa = 11.84NFIDD71 pKa = 4.14VPCGKK76 pKa = 9.81CVHH79 pKa = 6.22CLKK82 pKa = 10.58NKK84 pKa = 9.67RR85 pKa = 11.84RR86 pKa = 11.84EE87 pKa = 3.91LSFRR91 pKa = 11.84IYY93 pKa = 11.1NEE95 pKa = 3.66LRR97 pKa = 11.84DD98 pKa = 3.83AEE100 pKa = 4.25SAYY103 pKa = 10.49FVTLTYY109 pKa = 7.79DD110 pKa = 3.5TPNLQFNLHH119 pKa = 6.41SGLPILVKK127 pKa = 10.3RR128 pKa = 11.84DD129 pKa = 3.21VQLFLKK135 pKa = 10.43RR136 pKa = 11.84LRR138 pKa = 11.84KK139 pKa = 9.72DD140 pKa = 3.42LSSKK144 pKa = 10.95GLDD147 pKa = 3.42HH148 pKa = 7.56KK149 pKa = 10.66NLKK152 pKa = 8.86YY153 pKa = 10.72FCVSEE158 pKa = 4.43FGDD161 pKa = 3.5EE162 pKa = 4.23FMRR165 pKa = 11.84PHH167 pKa = 6.69YY168 pKa = 10.85HH169 pKa = 6.54MILFNLPIQDD179 pKa = 3.88IPVTTAKK186 pKa = 9.9RR187 pKa = 11.84CYY189 pKa = 9.88YY190 pKa = 9.83PVIKK194 pKa = 10.36QYY196 pKa = 11.31LDD198 pKa = 4.5SIWQLGFTDD207 pKa = 4.11VGCVTLASCNYY218 pKa = 8.02CAKK221 pKa = 9.92YY222 pKa = 9.49VCKK225 pKa = 10.53GLFNLDD231 pKa = 3.19EE232 pKa = 4.73KK233 pKa = 10.96PWVNEE238 pKa = 3.39YY239 pKa = 7.2WTLRR243 pKa = 11.84SQGIGLNFVDD253 pKa = 4.43KK254 pKa = 11.02YY255 pKa = 11.27GNRR258 pKa = 11.84FLKK261 pKa = 10.5SGKK264 pKa = 8.17TFTTWHH270 pKa = 6.2SFPIKK275 pKa = 10.26LPRR278 pKa = 11.84YY279 pKa = 8.03YY280 pKa = 10.06INKK283 pKa = 8.96IFYY286 pKa = 11.01NNDD289 pKa = 2.82SDD291 pKa = 5.6NFHH294 pKa = 4.95QHH296 pKa = 5.82CRR298 pKa = 11.84LSSKK302 pKa = 9.79AWNYY306 pKa = 10.98NNTAGNDD313 pKa = 3.3AYY315 pKa = 11.37SYY317 pKa = 10.7FLRR320 pKa = 11.84ASNRR324 pKa = 11.84QFEE327 pKa = 4.22NAAILFGQYY336 pKa = 7.44QHH338 pKa = 6.44ATVNNVLRR346 pKa = 11.84ASGGFRR352 pKa = 11.84ITNVRR357 pKa = 3.32

Molecular weight: 41.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1481

112

593

296.2

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.617 ± 1.169

1.215 ± 0.584

5.807 ± 1.718

4.186 ± 0.724

5.739 ± 0.929

5.874 ± 0.873

2.026 ± 0.569

5.672 ± 0.503

4.727 ± 0.73

8.778 ± 0.755

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.576

6.955 ± 1.466

4.929 ± 0.915

5.199 ± 1.134

5.537 ± 0.252

7.427 ± 0.88

4.862 ± 0.58

5.604 ± 0.916

1.148 ± 0.3

5.132 ± 0.688
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski