Simkania negevensis (strain ATCC VR-1471 / Z)
Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Parachlamydiales; Simkaniaceae; Simkania; Simkania negevensis
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Virtual 2D-PAGE plot for 2516 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F8L3J4|F8L3J4_SIMNZ Uncharacterized protein OS=Simkania negevensis (strain ATCC VR-1471 / Z) OX=331113 GN=SNE_A19760 PE=4 SV=1
MM1 pKa = 7.68WALCDD6 pKa = 3.7TTMKK10 pKa = 10.61AQLSTGQVMNSNALIANALSVLMDD34 pKa = 4.76ALSQAYY40 pKa = 10.17VGDD43 pKa = 3.99PNAPFTPDD51 pKa = 3.32EE52 pKa = 5.15DD53 pKa = 4.02PTQGSISGHH62 pKa = 4.41TLSWYY67 pKa = 9.3VDD69 pKa = 3.31EE70 pKa = 4.5MQYY73 pKa = 11.44LAGKK77 pKa = 9.4AGKK80 pKa = 8.46DD81 pKa = 3.2TDD83 pKa = 3.57QKK85 pKa = 11.57LSFEE89 pKa = 4.24MSFVQQTYY97 pKa = 10.86SLTNTSMQEE106 pKa = 3.95NVNVLNNSTQAEE118 pKa = 4.41SQQTQQDD125 pKa = 3.35NSALQGLITLASTGNGSATYY145 pKa = 9.91AANLMQQTMAA155 pKa = 4.63
MM1 pKa = 7.68WALCDD6 pKa = 3.7TTMKK10 pKa = 10.61AQLSTGQVMNSNALIANALSVLMDD34 pKa = 4.76ALSQAYY40 pKa = 10.17VGDD43 pKa = 3.99PNAPFTPDD51 pKa = 3.32EE52 pKa = 5.15DD53 pKa = 4.02PTQGSISGHH62 pKa = 4.41TLSWYY67 pKa = 9.3VDD69 pKa = 3.31EE70 pKa = 4.5MQYY73 pKa = 11.44LAGKK77 pKa = 9.4AGKK80 pKa = 8.46DD81 pKa = 3.2TDD83 pKa = 3.57QKK85 pKa = 11.57LSFEE89 pKa = 4.24MSFVQQTYY97 pKa = 10.86SLTNTSMQEE106 pKa = 3.95NVNVLNNSTQAEE118 pKa = 4.41SQQTQQDD125 pKa = 3.35NSALQGLITLASTGNGSATYY145 pKa = 9.91AANLMQQTMAA155 pKa = 4.63
Molecular weight: 16.66 kDa
Isoelectric point according different methods:
Protein with the highest isoelectric point:
>tr|F8L7Y9|F8L7Y9_SIMNZ Guanine deaminase OS=Simkania negevensis (strain ATCC VR-1471 / Z) OX=331113 GN=gDA PE=4 SV=1
MM1 pKa = 6.88PAKK4 pKa = 10.22RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.36KK9 pKa = 10.22AASSKK14 pKa = 8.9KK15 pKa = 4.49TTRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 8.04ATKK24 pKa = 8.52TAAKK28 pKa = 10.14KK29 pKa = 8.83RR30 pKa = 11.84TTRR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 6.1TAKK38 pKa = 10.14RR39 pKa = 11.84KK40 pKa = 4.96TAKK43 pKa = 10.29RR44 pKa = 11.84KK45 pKa = 4.86TAKK48 pKa = 10.29RR49 pKa = 11.84KK50 pKa = 4.86TAKK53 pKa = 10.29RR54 pKa = 11.84KK55 pKa = 4.9TAKK58 pKa = 10.06RR59 pKa = 11.84KK60 pKa = 7.62SAKK63 pKa = 9.65RR64 pKa = 11.84KK65 pKa = 8.1SARR68 pKa = 11.84KK69 pKa = 3.71TTRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 9.75KK75 pKa = 10.58AATAPAAATAAAAPKK90 pKa = 9.61KK91 pKa = 9.67RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84KK97 pKa = 9.16KK98 pKa = 10.04AAAKK102 pKa = 10.11
MM1 pKa = 6.88PAKK4 pKa = 10.22RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.36KK9 pKa = 10.22AASSKK14 pKa = 8.9KK15 pKa = 4.49TTRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 8.04ATKK24 pKa = 8.52TAAKK28 pKa = 10.14KK29 pKa = 8.83RR30 pKa = 11.84TTRR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 6.1TAKK38 pKa = 10.14RR39 pKa = 11.84KK40 pKa = 4.96TAKK43 pKa = 10.29RR44 pKa = 11.84KK45 pKa = 4.86TAKK48 pKa = 10.29RR49 pKa = 11.84KK50 pKa = 4.86TAKK53 pKa = 10.29RR54 pKa = 11.84KK55 pKa = 4.9TAKK58 pKa = 10.06RR59 pKa = 11.84KK60 pKa = 7.62SAKK63 pKa = 9.65RR64 pKa = 11.84KK65 pKa = 8.1SARR68 pKa = 11.84KK69 pKa = 3.71TTRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 9.75KK75 pKa = 10.58AATAPAAATAAAAPKK90 pKa = 9.61KK91 pKa = 9.67RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84RR96 pKa = 11.84KK97 pKa = 9.16KK98 pKa = 10.04AAAKK102 pKa = 10.11
Molecular weight: 11.59 kDa
Isoelectric point according different methods:
Peptides (in silico digests for buttom-up proteomics)
Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.| Try ESI |
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| ChTry ESI |
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| ArgC ESI |
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| LysN ESI |
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| TryLysC ESI |
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| Try MALDI |
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| ChTry MALDI |
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| ArgC MALDI |
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| LysN MALDI |
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| TryLysC MALDI |
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| Try LTQ |
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| ChTry LTQ |
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| ArgC LTQ |
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| LysN LTQ |
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| TryLysC LTQ |
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| Try MSlow |
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| ChTry MSlow |
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| ArgC MSlow |
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| LysN MSlow |
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| TryLysC MSlow |
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| Try MShigh |
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| ChTry MShigh |
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| ArgC MShigh |
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| LysN MShigh |
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| TryLysC MShigh |
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General Statistics
Number of major isoforms |
Number of additional isoforms |
Number of all proteins |
Number of amino acids |
Min. Seq. Length |
Max. Seq. Length |
Avg. Seq. Length |
Avg. Mol. Weight |
|---|---|---|---|---|---|---|---|
0 |
795211 |
16 |
4703 |
316.1 |
35.7 |
Amino acid frequency
Ala |
Cys |
Asp |
Glu |
Phe |
Gly |
His |
Ile |
Lys |
Leu |
|---|---|---|---|---|---|---|---|---|---|
6.81 ± 0.049 | 1.123 ± 0.015 |
4.822 ± 0.033 | 7.032 ± 0.067 |
5.322 ± 0.051 | 6.361 ± 0.097 |
2.521 ± 0.024 | 6.856 ± 0.043 |
6.888 ± 0.058 | 10.753 ± 0.061 |
Met |
Asn |
Gln |
Pro |
Arg |
Ser |
Thr |
Val |
Trp |
Tyr |
|---|---|---|---|---|---|---|---|---|---|
2.308 ± 0.024 | 3.894 ± 0.048 |
4.359 ± 0.034 | 4.028 ± 0.033 |
4.42 ± 0.045 | 6.682 ± 0.048 |
5.38 ± 0.072 | 5.834 ± 0.044 |
1.24 ± 0.021 | 3.361 ± 0.035 |
Most of the basic statistics you can see at this page can be downloaded from this CSV file
See this proteome in:
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here
| Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 | Contact: Lukasz P. Kozlowski |