Vibrio phage CHOED

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8FWV4|W8FWV4_9CAUD Uncharacterized protein OS=Vibrio phage CHOED OX=1458716 GN=CHOED_066 PE=4 SV=1
MM1 pKa = 7.11NKK3 pKa = 9.97AYY5 pKa = 9.64KK6 pKa = 10.41FSDD9 pKa = 3.16IDD11 pKa = 3.81SEE13 pKa = 4.76GMVVYY18 pKa = 10.46SSPKK22 pKa = 9.71LEE24 pKa = 5.0AGLGYY29 pKa = 9.92PKK31 pKa = 10.78GLVEE35 pKa = 5.06HH36 pKa = 6.87MGAHH40 pKa = 6.53FDD42 pKa = 5.01EE43 pKa = 5.98IDD45 pKa = 3.58LDD47 pKa = 4.11SPEE50 pKa = 4.21FTLEE54 pKa = 3.98GYY56 pKa = 8.46EE57 pKa = 4.0VHH59 pKa = 7.18IGQCDD64 pKa = 3.47MFEE67 pKa = 4.21MMFGNIQTIPCTAKK81 pKa = 10.11VYY83 pKa = 10.69RR84 pKa = 11.84LGKK87 pKa = 9.35PEE89 pKa = 5.05GYY91 pKa = 9.49PDD93 pKa = 4.02EE94 pKa = 6.47DD95 pKa = 4.33DD96 pKa = 4.6LFLVLSDD103 pKa = 3.78MGDD106 pKa = 3.24NTGVSFVGEE115 pKa = 4.7DD116 pKa = 3.52FLKK119 pKa = 10.87SYY121 pKa = 11.3DD122 pKa = 3.94PDD124 pKa = 3.66TGFTDD129 pKa = 3.16VTMIPNGQLGQFFADD144 pKa = 3.19WAEE147 pKa = 4.31IYY149 pKa = 10.7

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8FP51|W8FP51_9CAUD 5-3 exonuclease OS=Vibrio phage CHOED OX=1458716 GN=CHOED_034 PE=4 SV=1
MM1 pKa = 7.7LKK3 pKa = 9.41TKK5 pKa = 10.75LSYY8 pKa = 9.51TQISPDD14 pKa = 3.34GQMVWVLDD22 pKa = 4.13TLNHH26 pKa = 5.69ATGMMCYY33 pKa = 10.28YY34 pKa = 9.24STRR37 pKa = 11.84PTARR41 pKa = 11.84QIRR44 pKa = 11.84RR45 pKa = 11.84QQKK48 pKa = 10.07AVTKK52 pKa = 9.6NPWGNIVKK60 pKa = 10.15HH61 pKa = 4.86YY62 pKa = 10.25DD63 pKa = 2.64IRR65 pKa = 11.84TGKK68 pKa = 9.81FRR70 pKa = 4.53

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

19089

43

903

209.8

23.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.052 ± 0.42

0.891 ± 0.126

6.03 ± 0.212

6.58 ± 0.308

3.379 ± 0.143

7.826 ± 0.455

1.87 ± 0.155

5.537 ± 0.191

5.956 ± 0.201

7.984 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.023 ± 0.161

4.537 ± 0.215

4.102 ± 0.177

4.343 ± 0.315

4.275 ± 0.178

6.203 ± 0.31

6.36 ± 0.289

7.172 ± 0.231

1.304 ± 0.098

3.578 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski