Mongoose feces-associated gemycircularvirus c

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus humas3; Human associated gemykibivirus 3

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0E3JHI1|A0A0E3JHI1_9VIRU Replication-associated protein OS=Mongoose feces-associated gemycircularvirus c OX=1634488 PE=3 SV=1
MM1 pKa = 7.83SSFRR5 pKa = 11.84FQARR9 pKa = 11.84YY10 pKa = 10.11GLFTYY15 pKa = 7.62SQCGQLRR22 pKa = 11.84PSAVVEE28 pKa = 4.27LFSSLSADD36 pKa = 3.53CIVGRR41 pKa = 11.84EE42 pKa = 3.97AHH44 pKa = 6.62GDD46 pKa = 3.59GGTHH50 pKa = 6.01LHH52 pKa = 6.52AFVDD56 pKa = 4.76FRR58 pKa = 11.84RR59 pKa = 11.84KK60 pKa = 9.82YY61 pKa = 8.38RR62 pKa = 11.84TRR64 pKa = 11.84DD65 pKa = 3.08VRR67 pKa = 11.84KK68 pKa = 8.76FDD70 pKa = 3.82VEE72 pKa = 4.29GFHH75 pKa = 7.04PNIISTIRR83 pKa = 11.84SPSGSWDD90 pKa = 3.46YY91 pKa = 10.31ATKK94 pKa = 10.88DD95 pKa = 3.14GDD97 pKa = 3.76VCGGSLRR104 pKa = 11.84RR105 pKa = 11.84PEE107 pKa = 4.3PSQGSNAEE115 pKa = 4.02RR116 pKa = 11.84QNAGWDD122 pKa = 3.55QLRR125 pKa = 11.84DD126 pKa = 3.16ASTRR130 pKa = 11.84EE131 pKa = 3.56EE132 pKa = 4.16FLNLRR137 pKa = 11.84QRR139 pKa = 11.84IFSAISSNHH148 pKa = 5.62SLHH151 pKa = 6.82SLHH154 pKa = 5.94TQIGSLEE161 pKa = 4.38WIDD164 pKa = 3.88HH165 pKa = 6.68LIVTLANTRR174 pKa = 11.84SILHH178 pKa = 5.87NMVTFGNGLGDD189 pKa = 3.53IYY191 pKa = 11.45VWGPSRR197 pKa = 11.84TGKK200 pKa = 8.59TMWARR205 pKa = 11.84SLGKK209 pKa = 9.22HH210 pKa = 5.95AYY212 pKa = 9.85FGGLFSLEE220 pKa = 4.68EE221 pKa = 4.14YY222 pKa = 10.35EE223 pKa = 4.64QDD225 pKa = 3.58PDD227 pKa = 3.07CSYY230 pKa = 11.93AVFDD234 pKa = 5.63DD235 pKa = 3.64IQGGFKK241 pKa = 10.46FFPGYY246 pKa = 10.3KK247 pKa = 9.24SWLGQQTEE255 pKa = 5.26FYY257 pKa = 9.95CTDD260 pKa = 3.35RR261 pKa = 11.84YY262 pKa = 10.36RR263 pKa = 11.84KK264 pKa = 9.29KK265 pKa = 10.77KK266 pKa = 9.59HH267 pKa = 5.74IAWGKK272 pKa = 7.54PCIWLSNNNPMGEE285 pKa = 4.28DD286 pKa = 3.9ADD288 pKa = 4.7HH289 pKa = 7.46DD290 pKa = 4.16WLEE293 pKa = 4.16KK294 pKa = 10.65NCIIVYY300 pKa = 10.33VGDD303 pKa = 3.49RR304 pKa = 11.84LYY306 pKa = 11.41

Molecular weight:
34.94 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0E3JHI1|A0A0E3JHI1_9VIRU Replication-associated protein OS=Mongoose feces-associated gemycircularvirus c OX=1634488 PE=3 SV=1
MM1 pKa = 7.72AYY3 pKa = 10.24ARR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84FRR9 pKa = 11.84KK10 pKa = 7.9RR11 pKa = 11.84TYY13 pKa = 10.19ARR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84YY18 pKa = 6.01PTSRR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 9.45PSRR27 pKa = 11.84RR28 pKa = 11.84SYY30 pKa = 9.57GRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SSGRR39 pKa = 11.84SVRR42 pKa = 11.84SIRR45 pKa = 11.84NIAARR50 pKa = 11.84KK51 pKa = 9.51CRR53 pKa = 11.84DD54 pKa = 2.87NMMGVPVSDD63 pKa = 3.91TNIAGPPAPVQLTAGTDD80 pKa = 3.45YY81 pKa = 11.62GFLFCPSARR90 pKa = 11.84RR91 pKa = 11.84VGSLDD96 pKa = 2.88GSLPRR101 pKa = 11.84SIHH104 pKa = 5.9ASASEE109 pKa = 3.93RR110 pKa = 11.84WKK112 pKa = 8.76TRR114 pKa = 11.84CYY116 pKa = 10.5IKK118 pKa = 10.68GLQEE122 pKa = 4.18TIEE125 pKa = 4.05VQPTDD130 pKa = 2.94GMGWLWRR137 pKa = 11.84RR138 pKa = 11.84IVFSCIGLLDD148 pKa = 4.05EE149 pKa = 4.99FPGVSVSVADD159 pKa = 3.68NVNGYY164 pKa = 9.09GRR166 pKa = 11.84ALWNVRR172 pKa = 11.84SGTAEE177 pKa = 3.85AQPLFTEE184 pKa = 4.38TANYY188 pKa = 9.96LFEE191 pKa = 4.28GTRR194 pKa = 11.84FRR196 pKa = 11.84DD197 pKa = 3.21WVNPFTAKK205 pKa = 10.34LDD207 pKa = 3.75RR208 pKa = 11.84SIVTVHH214 pKa = 7.01ADD216 pKa = 3.38TVRR219 pKa = 11.84TVQSNNPNGTYY230 pKa = 10.2KK231 pKa = 10.39VYY233 pKa = 10.75KK234 pKa = 9.7RR235 pKa = 11.84YY236 pKa = 10.34YY237 pKa = 8.05PINRR241 pKa = 11.84GIVYY245 pKa = 10.49ADD247 pKa = 4.05DD248 pKa = 3.63EE249 pKa = 4.81SGAGKK254 pKa = 10.6KK255 pKa = 9.98NDD257 pKa = 4.2AIYY260 pKa = 10.51AAGTTKK266 pKa = 10.7GNSGDD271 pKa = 4.48LFVLDD276 pKa = 4.06MFSAVGDD283 pKa = 4.07NEE285 pKa = 4.4GSTLNFGVHH294 pKa = 6.13ARR296 pKa = 11.84YY297 pKa = 8.98YY298 pKa = 7.44WHH300 pKa = 7.27EE301 pKa = 4.21GSGQQ305 pKa = 3.18

Molecular weight:
34.27 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

611

305

306

305.5

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.71 ± 0.983

1.8 ± 0.323

6.056 ± 0.535

3.928 ± 0.429

4.91 ± 0.645

9.493 ± 0.01

2.619 ± 0.864

4.583 ± 0.429

3.764 ± 0.104

6.219 ± 0.86

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.473 ± 0.11

4.583 ± 0.438

3.928 ± 0.438

2.946 ± 0.647

9.82 ± 1.528

8.674 ± 0.316

5.565 ± 0.656

5.728 ± 0.982

2.455 ± 0.322

4.746 ± 0.33

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski