Rhinolophus simulator polyomavirus 3

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223YSI5|A0A223YSI5_9POLY Minor capsid protein VP2 OS=Rhinolophus simulator polyomavirus 3 OX=2029306 GN=VP3 PE=3 SV=1
MM1 pKa = 7.48APKK4 pKa = 9.99RR5 pKa = 11.84KK6 pKa = 9.71EE7 pKa = 4.15GEE9 pKa = 4.26CSKK12 pKa = 10.83SCPKK16 pKa = 10.32PSVVPRR22 pKa = 11.84LIVKK26 pKa = 10.26GGIEE30 pKa = 4.14VLSVKK35 pKa = 9.61TGPDD39 pKa = 2.85STTQIEE45 pKa = 4.84AYY47 pKa = 9.9LNPRR51 pKa = 11.84MGHH54 pKa = 5.88NLPTDD59 pKa = 3.23EE60 pKa = 5.22RR61 pKa = 11.84YY62 pKa = 10.39GYY64 pKa = 10.36SDD66 pKa = 3.56NVTVATSHH74 pKa = 6.74TDD76 pKa = 3.53DD77 pKa = 3.74NPKK80 pKa = 9.9IAEE83 pKa = 4.29LPTYY87 pKa = 9.88SAARR91 pKa = 11.84IALPMLNDD99 pKa = 4.69DD100 pKa = 4.4MTCSTLQMWEE110 pKa = 3.93AVSVKK115 pKa = 9.9TEE117 pKa = 3.91VVGASSLINGHH128 pKa = 5.5MFGKK132 pKa = 10.22RR133 pKa = 11.84VNNDD137 pKa = 2.98YY138 pKa = 11.48GIATPIEE145 pKa = 4.07GMNFHH150 pKa = 6.75MFAVGGEE157 pKa = 4.05PLEE160 pKa = 4.34LQAVVNNSRR169 pKa = 11.84TTWPAGTIGPKK180 pKa = 10.41GNSPKK185 pKa = 10.71LQVLDD190 pKa = 3.95PTAKK194 pKa = 10.61AKK196 pKa = 10.37LDD198 pKa = 3.49KK199 pKa = 11.2DD200 pKa = 3.44GAYY203 pKa = 9.58PIEE206 pKa = 4.6AWSPDD211 pKa = 3.13PSKK214 pKa = 11.56NEE216 pKa = 3.36NTRR219 pKa = 11.84YY220 pKa = 9.17YY221 pKa = 11.16GSYY224 pKa = 9.72TGGTTTPPVVQFTNTVTTVLLDD246 pKa = 3.84EE247 pKa = 5.12NGVGPLCKK255 pKa = 10.08GDD257 pKa = 4.05GLFLTAADD265 pKa = 3.01IVGFFTDD272 pKa = 2.94SSGYY276 pKa = 8.77QSYY279 pKa = 10.8RR280 pKa = 11.84GLPRR284 pKa = 11.84YY285 pKa = 10.0FNVQLRR291 pKa = 11.84KK292 pKa = 9.61RR293 pKa = 11.84VVKK296 pKa = 10.6NPYY299 pKa = 9.32PVTSLLSSLFCNLMPQITGQPMEE322 pKa = 4.75GNDD325 pKa = 3.84GQVEE329 pKa = 4.38EE330 pKa = 4.24VRR332 pKa = 11.84VYY334 pKa = 10.65QGLEE338 pKa = 4.21GVSGDD343 pKa = 3.8PDD345 pKa = 3.27MEE347 pKa = 4.37RR348 pKa = 11.84YY349 pKa = 8.96VDD351 pKa = 4.97KK352 pKa = 10.99FGQEE356 pKa = 3.75QTNIPGGACIRR367 pKa = 11.84PP368 pKa = 3.6

Molecular weight: 39.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223Z975|A0A223Z975_9POLY Small T antigen OS=Rhinolophus simulator polyomavirus 3 OX=2029306 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 6.23LLHH5 pKa = 6.73GSSGGMTSTEE15 pKa = 3.76NGMTSSVMRR24 pKa = 11.84PSAAVMKK31 pKa = 10.34RR32 pKa = 11.84SNLEE36 pKa = 3.49EE37 pKa = 4.54DD38 pKa = 3.62PAEE41 pKa = 4.0IALNMIVGSTLDD53 pKa = 3.69PLRR56 pKa = 11.84GPLLHH61 pKa = 7.04PLNPRR66 pKa = 11.84NKK68 pKa = 9.28KK69 pKa = 8.82PSMYY73 pKa = 10.48LRR75 pKa = 11.84IFLVSLIVSLVMLFIVIRR93 pKa = 11.84QFVHH97 pKa = 6.47FF98 pKa = 4.64

Molecular weight: 10.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1827

98

663

304.5

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.185 ± 1.377

2.189 ± 0.889

4.926 ± 0.528

6.897 ± 0.411

3.941 ± 0.755

6.568 ± 0.834

2.518 ± 0.403

4.324 ± 0.225

6.513 ± 1.74

9.852 ± 0.797

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.175 ± 0.522

4.488 ± 0.539

5.802 ± 0.606

3.612 ± 0.389

4.762 ± 0.997

6.24 ± 0.487

5.473 ± 0.671

6.623 ± 0.642

1.861 ± 0.486

4.05 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski