Coccidioides posadasii (strain RMSCC 757 / Silveira) (Valley fever fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Onygenales incertae sedis; Coccidioides; Coccidioides posadasii

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9CTF7|E9CTF7_COCPS Predicted protein OS=Coccidioides posadasii (strain RMSCC 757 / Silveira) OX=443226 GN=CPSG_00848 PE=4 SV=1
MM1 pKa = 7.76ASIQTRR7 pKa = 11.84TDD9 pKa = 3.14VAPTGTSAVTSAVPTACGATTYY31 pKa = 10.2DD32 pKa = 4.32IPTKK36 pKa = 10.53DD37 pKa = 3.26AACAVPGTEE46 pKa = 5.17HH47 pKa = 7.14KK48 pKa = 10.98DD49 pKa = 3.73SMEE52 pKa = 4.27KK53 pKa = 10.76CCDD56 pKa = 3.65APVVTYY62 pKa = 11.16NEE64 pKa = 4.46GCGMYY69 pKa = 10.13CLASGGTVGDD79 pKa = 4.12LVKK82 pKa = 10.84CLIGDD87 pKa = 4.79GIADD91 pKa = 3.57GKK93 pKa = 9.37VFCNKK98 pKa = 8.87EE99 pKa = 3.83MNATATTTPTPTGRR113 pKa = 11.84DD114 pKa = 3.53DD115 pKa = 6.59DD116 pKa = 5.66DD117 pKa = 5.7DD118 pKa = 6.8HH119 pKa = 9.25DD120 pKa = 6.63DD121 pKa = 5.97DD122 pKa = 7.11DD123 pKa = 6.29DD124 pKa = 4.68EE125 pKa = 4.58PTNTDD130 pKa = 3.0GSPASTSSSAALANLPPQTLSKK152 pKa = 10.03PAIGVLFTLFFSTFAGVVFAA172 pKa = 6.13

Molecular weight: 17.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9DGH1|E9DGH1_COCPS Uncharacterized protein OS=Coccidioides posadasii (strain RMSCC 757 / Silveira) OX=443226 GN=CPSG_08920 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84SKK4 pKa = 9.75MCHH7 pKa = 4.7QRR9 pKa = 11.84HH10 pKa = 5.81FPRR13 pKa = 11.84TAALMPSASAHH24 pKa = 5.79LRR26 pKa = 11.84LSLKK30 pKa = 10.16QSPAAWLRR38 pKa = 11.84TARR41 pKa = 11.84RR42 pKa = 11.84QNSKK46 pKa = 10.82NPFVTQIKK54 pKa = 10.26SVVLLASPISRR65 pKa = 11.84RR66 pKa = 11.84APSAQPEE73 pKa = 4.52LPSQAAAAALRR84 pKa = 11.84PQPTPLLRR92 pKa = 11.84PQAAPRR98 pKa = 11.84ALALPHH104 pKa = 6.33PQALHH109 pKa = 5.34PTPTRR114 pKa = 11.84ILQATTLLKK123 pKa = 10.22PRR125 pKa = 11.84PPLPTGLRR133 pKa = 11.84LLLAMPMVVPSLPSPLL149 pKa = 3.76

Molecular weight: 16.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10209

3

10212

4174551

17

6196

408.8

45.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.0 ± 0.022

1.345 ± 0.01

5.483 ± 0.016

6.253 ± 0.028

3.726 ± 0.015

6.602 ± 0.022

2.472 ± 0.013

5.073 ± 0.018

5.075 ± 0.021

8.97 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.009

3.748 ± 0.011

6.276 ± 0.03

4.032 ± 0.017

6.593 ± 0.023

8.635 ± 0.031

5.681 ± 0.014

5.844 ± 0.016

1.377 ± 0.008

2.666 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski