Prevotella melaninogenica (strain ATCC 25845 / DSM 7089 / JCM 6325 / VPI 2381 / B282) (Bacteroides melaninogenicus)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella melaninogenica

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2288 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9RTK6|D9RTK6_PREMB 30S ribosomal protein S10 OS=Prevotella melaninogenica (strain ATCC 25845 / DSM 7089 / JCM 6325 / VPI 2381 / B282) OX=553174 GN=rpsJ PE=3 SV=1
MM1 pKa = 7.92KK2 pKa = 10.69YY3 pKa = 10.22QITCDD8 pKa = 3.14NCGTQFIVEE17 pKa = 4.22AEE19 pKa = 3.99EE20 pKa = 4.55GQTIEE25 pKa = 4.62CTCPHH30 pKa = 6.43CHH32 pKa = 5.73GVMEE36 pKa = 4.37VTLPLVSAGQQYY48 pKa = 7.41EE49 pKa = 4.12QPTGFTSQAQQVQGEE64 pKa = 4.44THH66 pKa = 6.77KK67 pKa = 10.83KK68 pKa = 10.07DD69 pKa = 3.38SNAILWGVVIGLLLLAGGVGAYY91 pKa = 7.39FTFGSSSPEE100 pKa = 3.95TPVTDD105 pKa = 5.17SIPNDD110 pKa = 3.9TIPYY114 pKa = 7.19EE115 pKa = 4.2SPIQVEE121 pKa = 4.16QTPSVDD127 pKa = 3.66TVATAPAEE135 pKa = 4.13PEE137 pKa = 4.06QKK139 pKa = 10.39EE140 pKa = 4.17EE141 pKa = 4.2EE142 pKa = 4.35QVEE145 pKa = 4.23EE146 pKa = 4.17QPEE149 pKa = 4.09EE150 pKa = 4.18GADD153 pKa = 3.76TIAVPGHH160 pKa = 6.98DD161 pKa = 3.62EE162 pKa = 3.81

Molecular weight: 17.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9RWJ4|D9RWJ4_PREMB Abi-like protein OS=Prevotella melaninogenica (strain ATCC 25845 / DSM 7089 / JCM 6325 / VPI 2381 / B282) OX=553174 GN=HMPREF0659_A7033 PE=4 SV=1
MM1 pKa = 8.27RR2 pKa = 11.84YY3 pKa = 9.78RR4 pKa = 11.84YY5 pKa = 8.36TEE7 pKa = 4.34GGDD10 pKa = 3.03VRR12 pKa = 11.84YY13 pKa = 9.03FFKK16 pKa = 10.9SIVIATKK23 pKa = 10.14CNRR26 pKa = 11.84LRR28 pKa = 11.84VSGMRR33 pKa = 11.84NDD35 pKa = 3.56EE36 pKa = 3.91VLGRR40 pKa = 11.84LNRR43 pKa = 11.84FKK45 pKa = 11.29DD46 pKa = 3.59NLIMSSCLNPKK57 pKa = 9.37NRR59 pKa = 11.84QLL61 pKa = 3.69

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2288

0

2288

839578

37

3011

366.9

41.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.168 ± 0.05

1.145 ± 0.018

5.76 ± 0.034

6.236 ± 0.053

4.448 ± 0.031

6.731 ± 0.049

2.012 ± 0.02

6.722 ± 0.05

6.937 ± 0.041

8.949 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.024

5.277 ± 0.041

3.595 ± 0.022

3.69 ± 0.031

4.624 ± 0.039

6.121 ± 0.043

5.745 ± 0.043

6.488 ± 0.037

1.213 ± 0.019

4.412 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski