Caulobacteraceae bacterium OTSz_A_272

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; unclassified Caulobacteraceae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3743 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1AKB8|A0A1B1AKB8_9CAUL FolB domain-containing protein OS=Caulobacteraceae bacterium OTSz_A_272 OX=1759059 GN=ATE48_14310 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84NWIIGAAALLAVAAPSVAAAQTGYY26 pKa = 11.2VGVNYY31 pKa = 10.6GNVDD35 pKa = 2.94AGGGVDD41 pKa = 3.46EE42 pKa = 5.75DD43 pKa = 5.39FYY45 pKa = 11.89GLDD48 pKa = 3.44GAVAFAGSGSIVFEE62 pKa = 3.67VDD64 pKa = 2.88AAVVDD69 pKa = 4.79SDD71 pKa = 4.98DD72 pKa = 3.43TDD74 pKa = 3.04TGYY77 pKa = 11.79GLTGHH82 pKa = 6.59VYY84 pKa = 10.52SRR86 pKa = 11.84NDD88 pKa = 2.74SHH90 pKa = 8.46LFGGFIGVADD100 pKa = 4.49SNDD103 pKa = 3.08STTWVGGLEE112 pKa = 3.87AAKK115 pKa = 10.72YY116 pKa = 5.31YY117 pKa = 9.75TNWTLAGAVFYY128 pKa = 11.24GNNDD132 pKa = 3.59DD133 pKa = 5.55ADD135 pKa = 3.64VDD137 pKa = 4.57GYY139 pKa = 10.98GLNVEE144 pKa = 4.35ARR146 pKa = 11.84AFVSDD151 pKa = 3.88NFRR154 pKa = 11.84LNANIGYY161 pKa = 10.47ANVDD165 pKa = 3.28AGAGDD170 pKa = 4.87DD171 pKa = 3.8DD172 pKa = 5.26AMIYY176 pKa = 10.54GVGGEE181 pKa = 4.18YY182 pKa = 10.34QFAAMPISIVAGYY195 pKa = 7.92STTDD199 pKa = 3.55FDD201 pKa = 5.37GGDD204 pKa = 3.61VDD206 pKa = 3.97TLSIGVRR213 pKa = 11.84YY214 pKa = 9.61NWGGTLRR221 pKa = 11.84DD222 pKa = 3.72RR223 pKa = 11.84DD224 pKa = 3.47RR225 pKa = 11.84SGASQASLTGVGSVFF240 pKa = 3.69

Molecular weight: 24.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1AJZ8|A0A1B1AJZ8_9CAUL MFS transporter OS=Caulobacteraceae bacterium OTSz_A_272 OX=1759059 GN=ATE48_13710 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 10.61LNLLHH7 pKa = 6.94RR8 pKa = 11.84WFGALVEE15 pKa = 4.54SDD17 pKa = 3.59DD18 pKa = 4.99HH19 pKa = 7.03GVTLLRR25 pKa = 11.84FLAVGGTATLVMMLLSISFSSGFGFPPQVAQGVAHH60 pKa = 7.41ALCIVPTYY68 pKa = 10.27LCQRR72 pKa = 11.84AITFRR77 pKa = 11.84SNVQHH82 pKa = 6.88RR83 pKa = 11.84RR84 pKa = 11.84GLLGYY89 pKa = 10.8LSMQLPLLGMGVALAWLLIGQMHH112 pKa = 7.08WPRR115 pKa = 11.84EE116 pKa = 3.88IGLATIAVIVGATSFLVQRR135 pKa = 11.84LVIFASKK142 pKa = 9.51SQKK145 pKa = 10.52

Molecular weight: 15.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3743

0

3743

1113520

53

2768

297.5

32.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.662 ± 0.065

0.804 ± 0.014

5.661 ± 0.034

5.892 ± 0.032

3.798 ± 0.023

8.47 ± 0.051

1.895 ± 0.019

5.029 ± 0.029

2.911 ± 0.048

9.582 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.02

2.821 ± 0.027

5.074 ± 0.034

3.367 ± 0.026

7.461 ± 0.044

5.358 ± 0.029

5.112 ± 0.039

7.121 ± 0.031

1.433 ± 0.017

2.204 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski