Raphidocelis subcapitata

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Sphaeropleales; Selenastraceae; Raphidocelis

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V0NJQ8|A0A2V0NJQ8_9CHLO Uncharacterized protein OS=Raphidocelis subcapitata OX=307507 GN=Rsub_00185 PE=4 SV=1
MM1 pKa = 8.11ADD3 pKa = 3.46PCSQQHH9 pKa = 6.69ADD11 pKa = 3.81RR12 pKa = 11.84EE13 pKa = 4.56AAGGPGTAAPAAAALDD29 pKa = 3.81AAAATDD35 pKa = 3.69QDD37 pKa = 3.7QLPRR41 pKa = 11.84SSVKK45 pKa = 9.79WFLNPVAAASSTQEE59 pKa = 3.63GDD61 pKa = 2.99EE62 pKa = 4.61DD63 pKa = 4.46GGGEE67 pKa = 4.27EE68 pKa = 5.05DD69 pKa = 5.12GEE71 pKa = 4.64DD72 pKa = 3.46GGAPRR77 pKa = 11.84DD78 pKa = 4.05NGSSAAGAGGTAAPSPTLSLPSEE101 pKa = 4.48CSAGVDD107 pKa = 3.31VDD109 pKa = 4.31ADD111 pKa = 3.83AGTGLAPCASEE122 pKa = 4.63SAASCGSPAASLGGSAASSAGCAPAYY148 pKa = 9.08CARR151 pKa = 11.84PGSGPEE157 pKa = 4.22DD158 pKa = 3.58ALEE161 pKa = 4.03LAGEE165 pKa = 4.48GLSPAASEE173 pKa = 4.33AVVTVASEE181 pKa = 4.05HH182 pKa = 6.03MGGCGSARR190 pKa = 11.84TVGGGSVGSTSSDD203 pKa = 3.21GSDD206 pKa = 4.36DD207 pKa = 3.36SATAHH212 pKa = 6.01GRR214 pKa = 11.84SSSFGCDD221 pKa = 2.69AAASAAGLLSPQTHH235 pKa = 5.29QAACDD240 pKa = 3.47EE241 pKa = 4.83TEE243 pKa = 4.33PQLQLAPAPDD253 pKa = 3.79ARR255 pKa = 11.84VCSAGEE261 pKa = 4.12GGASSAHH268 pKa = 5.98ASISGGSPGISGRR281 pKa = 11.84HH282 pKa = 5.36SDD284 pKa = 3.72SSATSGGGGDD294 pKa = 4.12CASSGSNSDD303 pKa = 3.4GGSGATVQVAGTAHH317 pKa = 7.87ALPPAAGAQPPMDD330 pKa = 4.48GADD333 pKa = 3.27ALL335 pKa = 4.13

Molecular weight: 30.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V0P142|A0A2V0P142_9CHLO Ubiquitin carboxyl-terminal hydrolase OS=Raphidocelis subcapitata OX=307507 GN=Rsub_06021 PE=3 SV=1
MM1 pKa = 6.88QAMLRR6 pKa = 11.84GVAFTSRR13 pKa = 11.84VGGAPVAARR22 pKa = 11.84RR23 pKa = 11.84PQAVALAPRR32 pKa = 11.84PTSLVAPSFTGGAFSGSILAPSRR55 pKa = 11.84PQGRR59 pKa = 11.84AALTVVAAGGRR70 pKa = 11.84SMGCTLQGSRR80 pKa = 11.84RR81 pKa = 11.84KK82 pKa = 9.82AKK84 pKa = 8.76RR85 pKa = 11.84TSGFRR90 pKa = 11.84ARR92 pKa = 11.84MRR94 pKa = 11.84SANGRR99 pKa = 11.84RR100 pKa = 11.84VLASRR105 pKa = 11.84RR106 pKa = 11.84KK107 pKa = 9.22RR108 pKa = 11.84GRR110 pKa = 11.84KK111 pKa = 8.79VLSGSSAPRR120 pKa = 11.84TGGKK124 pKa = 9.54

Molecular weight: 12.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13347

0

13347

7490027

56

7921

561.2

57.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

19.358 ± 0.057

1.43 ± 0.011

4.476 ± 0.013

5.288 ± 0.023

2.29 ± 0.01

11.612 ± 0.032

1.739 ± 0.009

1.962 ± 0.011

2.372 ± 0.017

9.071 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.5 ± 0.008

1.511 ± 0.009

7.477 ± 0.028

4.418 ± 0.031

7.391 ± 0.021

6.103 ± 0.019

3.541 ± 0.016

5.591 ± 0.016

1.336 ± 0.008

1.536 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski