Mycobacterium phage Lucyedi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514U3U8|A0A514U3U8_9CAUD Uncharacterized protein OS=Mycobacterium phage Lucyedi OX=2593349 GN=91 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.36PVKK6 pKa = 10.63EE7 pKa = 4.24FAEE10 pKa = 4.43VWSDD14 pKa = 3.22GNLAYY19 pKa = 9.92EE20 pKa = 4.18VGPTLSCTEE29 pKa = 4.04AEE31 pKa = 4.37ALASLLLGIGKK42 pKa = 9.05PEE44 pKa = 4.41AAEE47 pKa = 3.77AWINSHH53 pKa = 6.9ASSDD57 pKa = 3.74DD58 pKa = 3.64CGDD61 pKa = 3.45IHH63 pKa = 6.29CTCSDD68 pKa = 3.62CHH70 pKa = 7.22PEE72 pKa = 4.0VTISTEE78 pKa = 3.98SPKK81 pKa = 11.08

Molecular weight: 8.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514U3E9|A0A514U3E9_9CAUD Ribonucleoside-triphosphate reductase (thioredoxin) OS=Mycobacterium phage Lucyedi OX=2593349 GN=52 PE=3 SV=1
MM1 pKa = 8.16SEE3 pKa = 3.89EE4 pKa = 4.15DD5 pKa = 3.66SRR7 pKa = 11.84TVSAKK12 pKa = 10.35HH13 pKa = 4.78RR14 pKa = 11.84MCRR17 pKa = 11.84VFQHH21 pKa = 5.9AWDD24 pKa = 3.84YY25 pKa = 7.77TTVKK29 pKa = 10.37RR30 pKa = 11.84DD31 pKa = 3.21GANYY35 pKa = 10.06LQGLVCIRR43 pKa = 11.84CGTEE47 pKa = 3.04RR48 pKa = 11.84FMKK51 pKa = 9.96IDD53 pKa = 3.32ARR55 pKa = 11.84TGEE58 pKa = 4.38AGGSKK63 pKa = 10.18YY64 pKa = 10.54KK65 pKa = 10.92YY66 pKa = 10.04ADD68 pKa = 3.62GYY70 pKa = 10.21LFKK73 pKa = 11.09GGGALTVGEE82 pKa = 4.29RR83 pKa = 11.84AALRR87 pKa = 11.84LIEE90 pKa = 4.29VSGHH94 pKa = 4.75LPRR97 pKa = 11.84RR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84KK101 pKa = 9.42SS102 pKa = 3.14

Molecular weight: 11.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

16437

34

1020

174.9

19.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.57 ± 0.327

0.925 ± 0.118

6.583 ± 0.149

6.856 ± 0.255

3.346 ± 0.144

8.122 ± 0.435

1.995 ± 0.178

5.056 ± 0.178

4.934 ± 0.282

7.903 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.143

3.34 ± 0.169

5.548 ± 0.278

3.389 ± 0.206

6.364 ± 0.291

5.95 ± 0.174

5.895 ± 0.211

7.209 ± 0.272

1.989 ± 0.116

2.713 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski