Amino acid dipepetide frequency for Rheinheimera sp. F8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.759AlaAla: 12.759 ± 0.157	1.057AlaCys: 1.057 ± 0.036	6.548AlaAsp: 6.548 ± 0.084	6.86AlaGlu: 6.86 ± 0.09	3.516AlaPhe: 3.516 ± 0.068	8.278AlaGly: 8.278 ± 0.092	1.805AlaHis: 1.805 ± 0.046	5.172AlaIle: 5.172 ± 0.077	4.352AlaLys: 4.352 ± 0.076	13.076AlaLeu: 13.076 ± 0.156	2.631AlaMet: 2.631 ± 0.059	3.465AlaAsn: 3.465 ± 0.063	3.973AlaPro: 3.973 ± 0.069	5.755AlaGln: 5.755 ± 0.092	5.039AlaArg: 5.039 ± 0.074	5.595AlaSer: 5.595 ± 0.087	5.193AlaThr: 5.193 ± 0.086	7.563AlaVal: 7.563 ± 0.076	1.146AlaTrp: 1.146 ± 0.035	2.437AlaTyr: 2.437 ± 0.052	0.003AlaXaa: 0.003 ± 0.002
Cys	0.963CysAla: 0.963 ± 0.033	0.198CysCys: 0.198 ± 0.016	0.567CysAsp: 0.567 ± 0.026	0.402CysGlu: 0.402 ± 0.02	0.444CysPhe: 0.444 ± 0.024	0.86CysGly: 0.86 ± 0.033	0.293CysHis: 0.293 ± 0.016	0.466CysIle: 0.466 ± 0.023	0.317CysLys: 0.317 ± 0.018	1.004CysLeu: 1.004 ± 0.031	0.168CysMet: 0.168 ± 0.013	0.289CysAsn: 0.289 ± 0.015	0.493CysPro: 0.493 ± 0.02	0.68CysGln: 0.68 ± 0.031	0.601CysArg: 0.601 ± 0.026	0.731CysSer: 0.731 ± 0.029	0.436CysThr: 0.436 ± 0.02	0.537CysVal: 0.537 ± 0.023	0.222CysTrp: 0.222 ± 0.015	0.395CysTyr: 0.395 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	5.377AspAla: 5.377 ± 0.071	0.497AspCys: 0.497 ± 0.02	2.66AspAsp: 2.66 ± 0.06	2.97AspGlu: 2.97 ± 0.065	2.374AspPhe: 2.374 ± 0.046	3.996AspGly: 3.996 ± 0.066	1.045AspHis: 1.045 ± 0.035	3.144AspIle: 3.144 ± 0.065	2.515AspLys: 2.515 ± 0.053	5.847AspLeu: 5.847 ± 0.079	1.198AspMet: 1.198 ± 0.039	1.923AspAsn: 1.923 ± 0.043	2.343AspPro: 2.343 ± 0.045	2.483AspGln: 2.483 ± 0.053	2.284AspArg: 2.284 ± 0.049	3.013AspSer: 3.013 ± 0.062	2.427AspThr: 2.427 ± 0.05	3.596AspVal: 3.596 ± 0.072	1.016AspTrp: 1.016 ± 0.029	1.92AspTyr: 1.92 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	4.827GluAla: 4.827 ± 0.088	0.325GluCys: 0.325 ± 0.018	2.028GluAsp: 2.028 ± 0.049	2.1GluGlu: 2.1 ± 0.063	2.216GluPhe: 2.216 ± 0.051	2.518GluGly: 2.518 ± 0.055	1.293GluHis: 1.293 ± 0.038	2.825GluIle: 2.825 ± 0.053	2.394GluLys: 2.394 ± 0.048	7.163GluLeu: 7.163 ± 0.091	1.223GluMet: 1.223 ± 0.04	1.587GluAsn: 1.587 ± 0.039	2.123GluPro: 2.123 ± 0.053	4.376GluGln: 4.376 ± 0.078	3.02GluArg: 3.02 ± 0.055	2.284GluSer: 2.284 ± 0.054	2.514GluThr: 2.514 ± 0.054	3.498GluVal: 3.498 ± 0.076	0.588GluTrp: 0.588 ± 0.025	1.303GluTyr: 1.303 ± 0.036	0.002GluXaa: 0.002 ± 0.001
Phe	4.413PheAla: 4.413 ± 0.079	0.574PheCys: 0.574 ± 0.024	2.612PheAsp: 2.612 ± 0.055	1.834PheGlu: 1.834 ± 0.04	1.546PhePhe: 1.546 ± 0.042	3.199PheGly: 3.199 ± 0.058	0.8PheHis: 0.8 ± 0.028	2.172PheIle: 2.172 ± 0.049	1.572PheLys: 1.572 ± 0.041	3.341PheLeu: 3.341 ± 0.066	0.866PheMet: 0.866 ± 0.03	1.627PheAsn: 1.627 ± 0.04	1.396PhePro: 1.396 ± 0.04	1.498PheGln: 1.498 ± 0.039	1.998PheArg: 1.998 ± 0.049	3.195PheSer: 3.195 ± 0.057	2.002PheThr: 2.002 ± 0.044	2.676PheVal: 2.676 ± 0.057	0.616PheTrp: 0.616 ± 0.027	1.399PheTyr: 1.399 ± 0.04	0.002PheXaa: 0.002 ± 0.001
Gly	6.133GlyAla: 6.133 ± 0.101	0.793GlyCys: 0.793 ± 0.029	3.461GlyAsp: 3.461 ± 0.06	3.52GlyGlu: 3.52 ± 0.063	3.35GlyPhe: 3.35 ± 0.063	4.651GlyGly: 4.651 ± 0.093	1.557GlyHis: 1.557 ± 0.037	3.958GlyIle: 3.958 ± 0.068	3.582GlyLys: 3.582 ± 0.062	7.555GlyLeu: 7.555 ± 0.094	1.832GlyMet: 1.832 ± 0.048	2.257GlyAsn: 2.257 ± 0.054	2.106GlyPro: 2.106 ± 0.047	3.78GlyGln: 3.78 ± 0.066	3.559GlyArg: 3.559 ± 0.061	4.171GlySer: 4.171 ± 0.08	3.539GlyThr: 3.539 ± 0.076	4.903GlyVal: 4.903 ± 0.077	1.18GlyTrp: 1.18 ± 0.039	2.486GlyTyr: 2.486 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.885HisAla: 1.885 ± 0.047	0.318HisCys: 0.318 ± 0.016	0.968HisAsp: 0.968 ± 0.027	0.952HisGlu: 0.952 ± 0.033	1.057HisPhe: 1.057 ± 0.035	1.682HisGly: 1.682 ± 0.038	0.645HisHis: 0.645 ± 0.029	1.076HisIle: 1.076 ± 0.032	0.858HisLys: 0.858 ± 0.03	2.478HisLeu: 2.478 ± 0.05	0.415HisMet: 0.415 ± 0.018	0.743HisAsn: 0.743 ± 0.026	1.213HisPro: 1.213 ± 0.035	1.685HisGln: 1.685 ± 0.047	1.082HisArg: 1.082 ± 0.035	1.533HisSer: 1.533 ± 0.043	0.952HisThr: 0.952 ± 0.034	1.09HisVal: 1.09 ± 0.028	0.549HisTrp: 0.549 ± 0.025	0.854HisTyr: 0.854 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.545IleAla: 5.545 ± 0.076	0.618IleCys: 0.618 ± 0.024	3.243IleAsp: 3.243 ± 0.055	3.175IleGlu: 3.175 ± 0.058	1.637IlePhe: 1.637 ± 0.042	3.866IleGly: 3.866 ± 0.072	0.945IleHis: 0.945 ± 0.03	2.134IleIle: 2.134 ± 0.056	2.232IleLys: 2.232 ± 0.053	4.265IleLeu: 4.265 ± 0.079	0.838IleMet: 0.838 ± 0.035	2.068IleAsn: 2.068 ± 0.05	2.159IlePro: 2.159 ± 0.053	2.028IleGln: 2.028 ± 0.04	2.921IleArg: 2.921 ± 0.049	3.872IleSer: 3.872 ± 0.06	2.567IleThr: 2.567 ± 0.059	3.086IleVal: 3.086 ± 0.064	0.604IleTrp: 0.604 ± 0.026	1.392IleTyr: 1.392 ± 0.037	0.001IleXaa: 0.001 ± 0.001
Lys	4.802LysAla: 4.802 ± 0.08	0.218LysCys: 0.218 ± 0.017	2.154LysAsp: 2.154 ± 0.048	1.779LysGlu: 1.779 ± 0.056	1.395LysPhe: 1.395 ± 0.034	2.423LysGly: 2.423 ± 0.06	0.921LysHis: 0.921 ± 0.03	2.187LysIle: 2.187 ± 0.054	2.036LysLys: 2.036 ± 0.056	4.754LysLeu: 4.754 ± 0.067	1.013LysMet: 1.013 ± 0.031	1.492LysAsn: 1.492 ± 0.037	2.245LysPro: 2.245 ± 0.048	2.549LysGln: 2.549 ± 0.058	2.04LysArg: 2.04 ± 0.049	2.221LysSer: 2.221 ± 0.047	2.664LysThr: 2.664 ± 0.052	3.025LysVal: 3.025 ± 0.064	0.362LysTrp: 0.362 ± 0.017	1.028LysTyr: 1.028 ± 0.033	0.001LysXaa: 0.001 ± 0.001
Leu	13.262LeuAla: 13.262 ± 0.156	1.414LeuCys: 1.414 ± 0.046	5.864LeuAsp: 5.864 ± 0.092	5.492LeuGlu: 5.492 ± 0.083	4.358LeuPhe: 4.358 ± 0.076	7.183LeuGly: 7.183 ± 0.09	2.541LeuHis: 2.541 ± 0.051	5.151LeuIle: 5.151 ± 0.076	4.929LeuLys: 4.929 ± 0.089	15.197LeuLeu: 15.197 ± 0.243	2.623LeuMet: 2.623 ± 0.049	4.175LeuAsn: 4.175 ± 0.061	6.227LeuPro: 6.227 ± 0.085	8.6LeuGln: 8.6 ± 0.16	5.715LeuArg: 5.715 ± 0.087	7.974LeuSer: 7.974 ± 0.103	6.982LeuThr: 6.982 ± 0.092	7.608LeuVal: 7.608 ± 0.108	1.635LeuTrp: 1.635 ± 0.051	3.031LeuTyr: 3.031 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	2.572MetAla: 2.572 ± 0.056	0.16MetCys: 0.16 ± 0.013	0.949MetAsp: 0.949 ± 0.035	0.877MetGlu: 0.877 ± 0.033	0.794MetPhe: 0.794 ± 0.027	1.229MetGly: 1.229 ± 0.042	0.453MetHis: 0.453 ± 0.022	0.941MetIle: 0.941 ± 0.032	0.964MetLys: 0.964 ± 0.037	3.061MetLeu: 3.061 ± 0.055	0.556MetMet: 0.556 ± 0.028	0.762MetAsn: 0.762 ± 0.026	1.257MetPro: 1.257 ± 0.035	1.604MetGln: 1.604 ± 0.036	1.073MetArg: 1.073 ± 0.035	1.489MetSer: 1.489 ± 0.037	1.385MetThr: 1.385 ± 0.035	1.403MetVal: 1.403 ± 0.043	0.189MetTrp: 0.189 ± 0.014	0.519MetTyr: 0.519 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.34AsnAla: 3.34 ± 0.063	0.367AsnCys: 0.367 ± 0.019	1.7AsnAsp: 1.7 ± 0.045	1.58AsnGlu: 1.58 ± 0.04	1.385AsnPhe: 1.385 ± 0.042	2.604AsnGly: 2.604 ± 0.06	0.724AsnHis: 0.724 ± 0.029	1.836AsnIle: 1.836 ± 0.044	1.492AsnLys: 1.492 ± 0.041	3.348AsnLeu: 3.348 ± 0.056	0.711AsnMet: 0.711 ± 0.026	1.312AsnAsn: 1.312 ± 0.046	1.962AsnPro: 1.962 ± 0.042	2.016AsnGln: 2.016 ± 0.051	1.755AsnArg: 1.755 ± 0.046	2.178AsnSer: 2.178 ± 0.054	1.825AsnThr: 1.825 ± 0.047	2.054AsnVal: 2.054 ± 0.049	0.543AsnTrp: 0.543 ± 0.024	1.08AsnTyr: 1.08 ± 0.032	0.001AsnXaa: 0.001 ± 0.001
Pro	5.961ProAla: 5.961 ± 0.102	0.348ProCys: 0.348 ± 0.018	3.029ProAsp: 3.029 ± 0.058	2.967ProGlu: 2.967 ± 0.066	1.627ProPhe: 1.627 ± 0.042	3.26ProGly: 3.26 ± 0.065	0.928ProHis: 0.928 ± 0.032	1.69ProIle: 1.69 ± 0.046	1.629ProLys: 1.629 ± 0.043	5.355ProLeu: 5.355 ± 0.086	0.956ProMet: 0.956 ± 0.032	1.269ProAsn: 1.269 ± 0.037	1.481ProPro: 1.481 ± 0.045	2.668ProGln: 2.668 ± 0.056	1.651ProArg: 1.651 ± 0.041	2.07ProSer: 2.07 ± 0.048	2.131ProThr: 2.131 ± 0.06	4.033ProVal: 4.033 ± 0.062	0.602ProTrp: 0.602 ± 0.025	1.275ProTyr: 1.275 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	6.585GlnAla: 6.585 ± 0.116	0.468GlnCys: 0.468 ± 0.023	2.386GlnAsp: 2.386 ± 0.054	1.918GlnGlu: 1.918 ± 0.046	2.357GlnPhe: 2.357 ± 0.047	3.57GlnGly: 3.57 ± 0.06	2.055GlnHis: 2.055 ± 0.057	3.235GlnIle: 3.235 ± 0.06	2.073GlnLys: 2.073 ± 0.048	9.641GlnLeu: 9.641 ± 0.172	1.301GlnMet: 1.301 ± 0.036	1.705GlnAsn: 1.705 ± 0.047	3.14GlnPro: 3.14 ± 0.063	8.327GlnGln: 8.327 ± 0.221	3.55GlnArg: 3.55 ± 0.063	3.633GlnSer: 3.633 ± 0.078	2.903GlnThr: 2.903 ± 0.054	3.994GlnVal: 3.994 ± 0.065	1.062GlnTrp: 1.062 ± 0.034	1.777GlnTyr: 1.777 ± 0.049	0.001GlnXaa: 0.001 ± 0.001
Arg	4.362ArgAla: 4.362 ± 0.072	0.475ArgCys: 0.475 ± 0.021	2.706ArgAsp: 2.706 ± 0.053	2.691ArgGlu: 2.691 ± 0.056	2.401ArgPhe: 2.401 ± 0.05	2.816ArgGly: 2.816 ± 0.058	1.402ArgHis: 1.402 ± 0.038	2.802ArgIle: 2.802 ± 0.058	1.989ArgLys: 1.989 ± 0.046	6.548ArgLeu: 6.548 ± 0.093	1.174ArgMet: 1.174 ± 0.033	1.691ArgAsn: 1.691 ± 0.039	1.983ArgPro: 1.983 ± 0.048	3.922ArgGln: 3.922 ± 0.073	2.955ArgArg: 2.955 ± 0.053	2.78ArgSer: 2.78 ± 0.049	2.28ArgThr: 2.28 ± 0.054	3.158ArgVal: 3.158 ± 0.052	0.879ArgTrp: 0.879 ± 0.034	1.903ArgTyr: 1.903 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	6.814SerAla: 6.814 ± 0.097	0.619SerCys: 0.619 ± 0.025	3.116SerAsp: 3.116 ± 0.058	2.902SerGlu: 2.902 ± 0.064	2.567SerPhe: 2.567 ± 0.055	5.093SerGly: 5.093 ± 0.076	1.326SerHis: 1.326 ± 0.036	2.686SerIle: 2.686 ± 0.051	2.507SerLys: 2.507 ± 0.054	6.657SerLeu: 6.657 ± 0.088	1.412SerMet: 1.412 ± 0.036	2.089SerAsn: 2.089 ± 0.058	2.546SerPro: 2.546 ± 0.052	3.244SerGln: 3.244 ± 0.062	3.307SerArg: 3.307 ± 0.055	3.89SerSer: 3.89 ± 0.083	2.923SerThr: 2.923 ± 0.052	4.104SerVal: 4.104 ± 0.067	1.035SerTrp: 1.035 ± 0.035	2.005SerTyr: 2.005 ± 0.048	0.001SerXaa: 0.001 ± 0.001
Thr	5.985ThrAla: 5.985 ± 0.081	0.369ThrCys: 0.369 ± 0.02	3.09ThrAsp: 3.09 ± 0.057	3.161ThrGlu: 3.161 ± 0.064	1.7ThrPhe: 1.7 ± 0.04	4.237ThrGly: 4.237 ± 0.065	0.917ThrHis: 0.917 ± 0.036	2.433ThrIle: 2.433 ± 0.054	1.686ThrLys: 1.686 ± 0.048	6.298ThrLeu: 6.298 ± 0.082	0.967ThrMet: 0.967 ± 0.035	1.603ThrAsn: 1.603 ± 0.043	2.995ThrPro: 2.995 ± 0.065	2.375ThrGln: 2.375 ± 0.051	2.05ThrArg: 2.05 ± 0.05	2.874ThrSer: 2.874 ± 0.066	2.822ThrThr: 2.822 ± 0.079	3.946ThrVal: 3.946 ± 0.09	0.463ThrTrp: 0.463 ± 0.025	1.222ThrTyr: 1.222 ± 0.037	0.0ThrXaa: 0.0 ± 0.0
Val	7.24ValAla: 7.24 ± 0.1	0.69ValCys: 0.69 ± 0.026	3.469ValAsp: 3.469 ± 0.056	3.413ValGlu: 3.413 ± 0.063	2.65ValPhe: 2.65 ± 0.055	4.172ValGly: 4.172 ± 0.075	1.27ValHis: 1.27 ± 0.036	3.534ValIle: 3.534 ± 0.06	2.873ValLys: 2.873 ± 0.068	8.297ValLeu: 8.297 ± 0.108	1.579ValMet: 1.579 ± 0.043	2.419ValAsn: 2.419 ± 0.057	3.067ValPro: 3.067 ± 0.061	3.451ValGln: 3.451 ± 0.063	3.518ValArg: 3.518 ± 0.065	4.641ValSer: 4.641 ± 0.078	3.763ValThr: 3.763 ± 0.081	4.951ValVal: 4.951 ± 0.097	0.855ValTrp: 0.855 ± 0.03	1.742ValTyr: 1.742 ± 0.046	0.002ValXaa: 0.002 ± 0.001
Trp	0.9TrpAla: 0.9 ± 0.033	0.188TrpCys: 0.188 ± 0.016	0.575TrpAsp: 0.575 ± 0.024	0.439TrpGlu: 0.439 ± 0.022	0.726TrpPhe: 0.726 ± 0.027	0.703TrpGly: 0.703 ± 0.029	0.433TrpHis: 0.433 ± 0.019	0.542TrpIle: 0.542 ± 0.025	0.307TrpLys: 0.307 ± 0.019	2.373TrpLeu: 2.373 ± 0.059	0.281TrpMet: 0.281 ± 0.016	0.376TrpAsn: 0.376 ± 0.02	0.74TrpPro: 0.74 ± 0.032	1.814TrpGln: 1.814 ± 0.061	0.976TrpArg: 0.976 ± 0.034	0.847TrpSer: 0.847 ± 0.031	0.603TrpThr: 0.603 ± 0.026	0.738TrpVal: 0.738 ± 0.027	0.279TrpTrp: 0.279 ± 0.018	0.454TrpTyr: 0.454 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.497TyrAla: 2.497 ± 0.053	0.369TyrCys: 0.369 ± 0.02	1.498TyrAsp: 1.498 ± 0.048	1.175TyrGlu: 1.175 ± 0.037	1.238TyrPhe: 1.238 ± 0.038	2.027TyrGly: 2.027 ± 0.052	0.682TyrHis: 0.682 ± 0.026	1.212TyrIle: 1.212 ± 0.035	1.005TyrLys: 1.005 ± 0.038	3.699TyrLeu: 3.699 ± 0.061	0.492TyrMet: 0.492 ± 0.023	0.961TyrAsn: 0.961 ± 0.032	1.481TyrPro: 1.481 ± 0.041	2.827TyrGln: 2.827 ± 0.051	1.823TyrArg: 1.823 ± 0.046	1.852TyrSer: 1.852 ± 0.048	1.284TyrThr: 1.284 ± 0.049	1.632TyrVal: 1.632 ± 0.04	0.469TyrTrp: 0.469 ± 0.025	0.914TyrTyr: 0.914 ± 0.037	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.002XaaTyr: 0.002 ± 0.001	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 2872 proteins (1005369 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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