Amino acid dipepetide frequency for Clostridiales bacterium KA00134

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.095AlaAla: 4.095 ± 0.118	0.749AlaCys: 0.749 ± 0.042	3.424AlaAsp: 3.424 ± 0.09	3.915AlaGlu: 3.915 ± 0.094	2.817AlaPhe: 2.817 ± 0.088	4.382AlaGly: 4.382 ± 0.114	0.849AlaHis: 0.849 ± 0.041	5.598AlaIle: 5.598 ± 0.115	5.963AlaLys: 5.963 ± 0.123	6.588AlaLeu: 6.588 ± 0.122	1.792AlaMet: 1.792 ± 0.065	2.949AlaAsn: 2.949 ± 0.088	1.632AlaPro: 1.632 ± 0.067	1.919AlaGln: 1.919 ± 0.073	2.601AlaArg: 2.601 ± 0.066	3.985AlaSer: 3.985 ± 0.081	2.866AlaThr: 2.866 ± 0.093	3.501AlaVal: 3.501 ± 0.098	0.416AlaTrp: 0.416 ± 0.031	2.588AlaTyr: 2.588 ± 0.069	0.0AlaXaa: 0.0 ± 0.0
Cys	0.531CysAla: 0.531 ± 0.034	0.089CysCys: 0.089 ± 0.013	0.614CysAsp: 0.614 ± 0.041	0.616CysGlu: 0.616 ± 0.037	0.403CysPhe: 0.403 ± 0.028	0.904CysGly: 0.904 ± 0.043	0.257CysHis: 0.257 ± 0.027	0.709CysIle: 0.709 ± 0.042	0.777CysLys: 0.777 ± 0.047	0.957CysLeu: 0.957 ± 0.044	0.238CysMet: 0.238 ± 0.022	0.376CysAsn: 0.376 ± 0.027	0.45CysPro: 0.45 ± 0.036	0.244CysGln: 0.244 ± 0.019	0.284CysArg: 0.284 ± 0.023	0.52CysSer: 0.52 ± 0.032	0.299CysThr: 0.299 ± 0.023	0.543CysVal: 0.543 ± 0.037	0.053CysTrp: 0.053 ± 0.009	0.306CysTyr: 0.306 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	3.305AspAla: 3.305 ± 0.088	0.497AspCys: 0.497 ± 0.029	3.178AspAsp: 3.178 ± 0.092	5.263AspGlu: 5.263 ± 0.116	3.756AspPhe: 3.756 ± 0.1	4.042AspGly: 4.042 ± 0.103	0.749AspHis: 0.749 ± 0.042	5.36AspIle: 5.36 ± 0.101	6.377AspLys: 6.377 ± 0.137	6.689AspLeu: 6.689 ± 0.135	1.615AspMet: 1.615 ± 0.066	2.443AspAsn: 2.443 ± 0.078	1.864AspPro: 1.864 ± 0.074	1.471AspGln: 1.471 ± 0.064	2.276AspArg: 2.276 ± 0.074	3.312AspSer: 3.312 ± 0.093	2.475AspThr: 2.475 ± 0.083	3.422AspVal: 3.422 ± 0.092	0.386AspTrp: 0.386 ± 0.031	2.951AspTyr: 2.951 ± 0.076	0.0AspXaa: 0.0 ± 0.0
Glu	4.785GluAla: 4.785 ± 0.121	0.484GluCys: 0.484 ± 0.031	5.275GluAsp: 5.275 ± 0.111	7.01GluGlu: 7.01 ± 0.15	3.106GluPhe: 3.106 ± 0.081	4.335GluGly: 4.335 ± 0.105	0.771GluHis: 0.771 ± 0.037	7.454GluIle: 7.454 ± 0.164	9.188GluLys: 9.188 ± 0.144	6.392GluLeu: 6.392 ± 0.131	2.042GluMet: 2.042 ± 0.062	5.621GluAsn: 5.621 ± 0.116	1.437GluPro: 1.437 ± 0.061	1.535GluGln: 1.535 ± 0.053	2.775GluArg: 2.775 ± 0.09	3.632GluSer: 3.632 ± 0.094	2.651GluThr: 2.651 ± 0.085	4.896GluVal: 4.896 ± 0.113	0.352GluTrp: 0.352 ± 0.03	2.552GluTyr: 2.552 ± 0.069	0.0GluXaa: 0.0 ± 0.0
Phe	2.821PheAla: 2.821 ± 0.081	0.49PheCys: 0.49 ± 0.033	3.01PheAsp: 3.01 ± 0.082	3.531PheGlu: 3.531 ± 0.087	2.308PhePhe: 2.308 ± 0.096	2.777PheGly: 2.777 ± 0.079	0.539PheHis: 0.539 ± 0.035	4.059PheIle: 4.059 ± 0.109	4.248PheLys: 4.248 ± 0.088	4.69PheLeu: 4.69 ± 0.123	1.202PheMet: 1.202 ± 0.049	2.127PheAsn: 2.127 ± 0.066	1.246PhePro: 1.246 ± 0.049	1.059PheGln: 1.059 ± 0.043	1.616PheArg: 1.616 ± 0.05	3.409PheSer: 3.409 ± 0.086	2.376PheThr: 2.376 ± 0.076	2.874PheVal: 2.874 ± 0.081	0.321PheTrp: 0.321 ± 0.024	2.11PheTyr: 2.11 ± 0.076	0.0PheXaa: 0.0 ± 0.0
Gly	4.318GlyAla: 4.318 ± 0.103	0.628GlyCys: 0.628 ± 0.041	3.39GlyAsp: 3.39 ± 0.078	4.276GlyGlu: 4.276 ± 0.086	3.104GlyPhe: 3.104 ± 0.071	4.441GlyGly: 4.441 ± 0.11	1.078GlyHis: 1.078 ± 0.048	5.887GlyIle: 5.887 ± 0.128	6.309GlyLys: 6.309 ± 0.126	6.524GlyLeu: 6.524 ± 0.132	1.66GlyMet: 1.66 ± 0.058	3.008GlyAsn: 3.008 ± 0.085	1.378GlyPro: 1.378 ± 0.059	2.038GlyGln: 2.038 ± 0.069	2.658GlyArg: 2.658 ± 0.069	4.012GlySer: 4.012 ± 0.107	2.889GlyThr: 2.889 ± 0.098	4.002GlyVal: 4.002 ± 0.1	0.467GlyTrp: 0.467 ± 0.034	3.049GlyTyr: 3.049 ± 0.092	0.0GlyXaa: 0.0 ± 0.0
His	0.662HisAla: 0.662 ± 0.038	0.132HisCys: 0.132 ± 0.015	0.751HisAsp: 0.751 ± 0.041	0.858HisGlu: 0.858 ± 0.037	0.711HisPhe: 0.711 ± 0.035	0.885HisGly: 0.885 ± 0.043	0.248HisHis: 0.248 ± 0.024	1.089HisIle: 1.089 ± 0.04	1.019HisLys: 1.019 ± 0.048	1.252HisLeu: 1.252 ± 0.058	0.359HisMet: 0.359 ± 0.027	0.618HisAsn: 0.618 ± 0.032	0.611HisPro: 0.611 ± 0.034	0.367HisGln: 0.367 ± 0.024	0.58HisArg: 0.58 ± 0.038	0.79HisSer: 0.79 ± 0.044	0.597HisThr: 0.597 ± 0.034	0.671HisVal: 0.671 ± 0.035	0.104HisTrp: 0.104 ± 0.013	0.537HisTyr: 0.537 ± 0.036	0.0HisXaa: 0.0 ± 0.0
Ile	5.465IleAla: 5.465 ± 0.135	1.027IleCys: 1.027 ± 0.048	5.29IleAsp: 5.29 ± 0.102	6.551IleGlu: 6.551 ± 0.111	4.573IlePhe: 4.573 ± 0.116	5.431IleGly: 5.431 ± 0.122	1.027IleHis: 1.027 ± 0.05	7.297IleIle: 7.297 ± 0.155	8.169IleLys: 8.169 ± 0.134	9.014IleLeu: 9.014 ± 0.169	2.051IleMet: 2.051 ± 0.072	4.528IleAsn: 4.528 ± 0.099	2.696IlePro: 2.696 ± 0.072	1.906IleGln: 1.906 ± 0.059	3.45IleArg: 3.45 ± 0.092	6.632IleSer: 6.632 ± 0.131	3.652IleThr: 3.652 ± 0.103	5.021IleVal: 5.021 ± 0.106	0.484IleTrp: 0.484 ± 0.035	3.545IleTyr: 3.545 ± 0.087	0.0IleXaa: 0.0 ± 0.0
Lys	5.997LysAla: 5.997 ± 0.144	0.577LysCys: 0.577 ± 0.037	7.428LysAsp: 7.428 ± 0.138	8.902LysGlu: 8.902 ± 0.16	3.446LysPhe: 3.446 ± 0.088	5.212LysGly: 5.212 ± 0.105	1.042LysHis: 1.042 ± 0.047	8.74LysIle: 8.74 ± 0.132	9.783LysLys: 9.783 ± 0.149	7.969LysLeu: 7.969 ± 0.13	2.497LysMet: 2.497 ± 0.064	6.859LysAsn: 6.859 ± 0.121	2.218LysPro: 2.218 ± 0.079	2.059LysGln: 2.059 ± 0.066	3.794LysArg: 3.794 ± 0.103	5.902LysSer: 5.902 ± 0.12	5.152LysThr: 5.152 ± 0.106	5.388LysVal: 5.388 ± 0.129	0.599LysTrp: 0.599 ± 0.036	3.501LysTyr: 3.501 ± 0.079	0.0LysXaa: 0.0 ± 0.0
Leu	6.602LeuAla: 6.602 ± 0.134	0.855LeuCys: 0.855 ± 0.04	6.343LeuAsp: 6.343 ± 0.13	7.494LeuGlu: 7.494 ± 0.158	3.877LeuPhe: 3.877 ± 0.096	6.768LeuGly: 6.768 ± 0.125	1.059LeuHis: 1.059 ± 0.05	8.122LeuIle: 8.122 ± 0.183	9.566LeuLys: 9.566 ± 0.167	8.233LeuLeu: 8.233 ± 0.172	2.444LeuMet: 2.444 ± 0.073	4.923LeuAsn: 4.923 ± 0.113	3.093LeuPro: 3.093 ± 0.09	2.335LeuGln: 2.335 ± 0.071	3.603LeuArg: 3.603 ± 0.08	6.887LeuSer: 6.887 ± 0.137	4.394LeuThr: 4.394 ± 0.101	5.724LeuVal: 5.724 ± 0.116	0.518LeuTrp: 0.518 ± 0.033	3.013LeuTyr: 3.013 ± 0.079	0.0LeuXaa: 0.0 ± 0.0
Met	2.163MetAla: 2.163 ± 0.069	0.217MetCys: 0.217 ± 0.021	1.667MetAsp: 1.667 ± 0.061	1.951MetGlu: 1.951 ± 0.07	0.841MetPhe: 0.841 ± 0.04	1.949MetGly: 1.949 ± 0.064	0.278MetHis: 0.278 ± 0.024	2.093MetIle: 2.093 ± 0.079	2.528MetLys: 2.528 ± 0.066	2.068MetLeu: 2.068 ± 0.074	0.671MetMet: 0.671 ± 0.042	1.244MetAsn: 1.244 ± 0.05	0.934MetPro: 0.934 ± 0.048	0.785MetGln: 0.785 ± 0.035	0.976MetArg: 0.976 ± 0.043	1.511MetSer: 1.511 ± 0.046	1.301MetThr: 1.301 ± 0.045	1.488MetVal: 1.488 ± 0.056	0.136MetTrp: 0.136 ± 0.015	0.667MetTyr: 0.667 ± 0.036	0.0MetXaa: 0.0 ± 0.0
Asn	2.826AsnAla: 2.826 ± 0.073	0.399AsnCys: 0.399 ± 0.034	2.433AsnAsp: 2.433 ± 0.073	3.501AsnGlu: 3.501 ± 0.093	2.93AsnPhe: 2.93 ± 0.09	3.095AsnGly: 3.095 ± 0.111	0.705AsnHis: 0.705 ± 0.038	4.972AsnIle: 4.972 ± 0.092	5.093AsnLys: 5.093 ± 0.121	6.036AsnLeu: 6.036 ± 0.125	1.42AsnMet: 1.42 ± 0.047	2.495AsnAsn: 2.495 ± 0.091	2.365AsnPro: 2.365 ± 0.086	1.49AsnGln: 1.49 ± 0.061	1.951AsnArg: 1.951 ± 0.063	2.887AsnSer: 2.887 ± 0.097	2.293AsnThr: 2.293 ± 0.074	2.807AsnVal: 2.807 ± 0.072	0.355AsnTrp: 0.355 ± 0.027	2.227AsnTyr: 2.227 ± 0.085	0.0AsnXaa: 0.0 ± 0.0
Pro	1.7ProAla: 1.7 ± 0.063	0.244ProCys: 0.244 ± 0.022	1.83ProAsp: 1.83 ± 0.066	2.282ProGlu: 2.282 ± 0.071	1.361ProPhe: 1.361 ± 0.049	1.96ProGly: 1.96 ± 0.071	0.467ProHis: 0.467 ± 0.034	2.567ProIle: 2.567 ± 0.073	2.637ProLys: 2.637 ± 0.073	2.613ProLeu: 2.613 ± 0.074	0.69ProMet: 0.69 ± 0.037	1.478ProAsn: 1.478 ± 0.058	0.681ProPro: 0.681 ± 0.046	0.907ProGln: 0.907 ± 0.044	0.976ProArg: 0.976 ± 0.043	2.002ProSer: 2.002 ± 0.056	1.586ProThr: 1.586 ± 0.068	1.894ProVal: 1.894 ± 0.074	0.214ProTrp: 0.214 ± 0.02	1.212ProTyr: 1.212 ± 0.048	0.0ProXaa: 0.0 ± 0.0
Gln	2.013GlnAla: 2.013 ± 0.077	0.13GlnCys: 0.13 ± 0.015	1.632GlnAsp: 1.632 ± 0.063	2.21GlnGlu: 2.21 ± 0.075	0.834GlnPhe: 0.834 ± 0.04	1.63GlnGly: 1.63 ± 0.065	0.246GlnHis: 0.246 ± 0.021	2.543GlnIle: 2.543 ± 0.074	2.482GlnLys: 2.482 ± 0.081	1.701GlnLeu: 1.701 ± 0.057	0.728GlnMet: 0.728 ± 0.045	1.49GlnAsn: 1.49 ± 0.055	0.48GlnPro: 0.48 ± 0.034	0.49GlnGln: 0.49 ± 0.034	1.269GlnArg: 1.269 ± 0.048	1.287GlnSer: 1.287 ± 0.052	1.161GlnThr: 1.161 ± 0.054	1.87GlnVal: 1.87 ± 0.056	0.181GlnTrp: 0.181 ± 0.019	0.754GlnTyr: 0.754 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.592ArgAla: 2.592 ± 0.082	0.365ArgCys: 0.365 ± 0.03	2.322ArgAsp: 2.322 ± 0.071	3.293ArgGlu: 3.293 ± 0.097	1.662ArgPhe: 1.662 ± 0.054	2.471ArgGly: 2.471 ± 0.073	0.556ArgHis: 0.556 ± 0.031	3.418ArgIle: 3.418 ± 0.088	3.203ArgLys: 3.203 ± 0.077	3.719ArgLeu: 3.719 ± 0.084	0.977ArgMet: 0.977 ± 0.048	1.945ArgAsn: 1.945 ± 0.056	1.318ArgPro: 1.318 ± 0.053	1.272ArgGln: 1.272 ± 0.046	1.855ArgArg: 1.855 ± 0.071	2.172ArgSer: 2.172 ± 0.068	1.69ArgThr: 1.69 ± 0.057	2.603ArgVal: 2.603 ± 0.062	0.236ArgTrp: 0.236 ± 0.023	1.399ArgTyr: 1.399 ± 0.053	0.0ArgXaa: 0.0 ± 0.0
Ser	3.516SerAla: 3.516 ± 0.099	0.626SerCys: 0.626 ± 0.034	3.45SerAsp: 3.45 ± 0.088	3.83SerGlu: 3.83 ± 0.1	3.49SerPhe: 3.49 ± 0.084	4.516SerGly: 4.516 ± 0.103	0.945SerHis: 0.945 ± 0.045	5.352SerIle: 5.352 ± 0.111	6.023SerLys: 6.023 ± 0.125	7.074SerLeu: 7.074 ± 0.132	1.552SerMet: 1.552 ± 0.058	2.857SerAsn: 2.857 ± 0.076	1.86SerPro: 1.86 ± 0.063	1.754SerGln: 1.754 ± 0.068	2.384SerArg: 2.384 ± 0.073	4.331SerSer: 4.331 ± 0.115	2.669SerThr: 2.669 ± 0.068	3.397SerVal: 3.397 ± 0.09	0.42SerTrp: 0.42 ± 0.026	3.15SerTyr: 3.15 ± 0.083	0.0SerXaa: 0.0 ± 0.0
Thr	2.87ThrAla: 2.87 ± 0.093	0.522ThrCys: 0.522 ± 0.031	2.651ThrAsp: 2.651 ± 0.101	2.711ThrGlu: 2.711 ± 0.068	2.184ThrPhe: 2.184 ± 0.082	3.301ThrGly: 3.301 ± 0.101	0.667ThrHis: 0.667 ± 0.032	3.953ThrIle: 3.953 ± 0.1	3.734ThrLys: 3.734 ± 0.092	4.208ThrLeu: 4.208 ± 0.093	0.996ThrMet: 0.996 ± 0.046	2.095ThrAsn: 2.095 ± 0.069	1.722ThrPro: 1.722 ± 0.093	1.117ThrGln: 1.117 ± 0.047	1.868ThrArg: 1.868 ± 0.068	3.216ThrSer: 3.216 ± 0.081	2.15ThrThr: 2.15 ± 0.103	2.938ThrVal: 2.938 ± 0.098	0.352ThrTrp: 0.352 ± 0.029	1.855ThrTyr: 1.855 ± 0.062	0.0ThrXaa: 0.0 ± 0.0
Val	3.806ValAla: 3.806 ± 0.101	0.694ValCys: 0.694 ± 0.043	3.728ValAsp: 3.728 ± 0.093	4.632ValGlu: 4.632 ± 0.112	3.102ValPhe: 3.102 ± 0.09	4.029ValGly: 4.029 ± 0.104	0.762ValHis: 0.762 ± 0.039	4.791ValIle: 4.791 ± 0.102	5.322ValLys: 5.322 ± 0.119	5.828ValLeu: 5.828 ± 0.115	1.401ValMet: 1.401 ± 0.055	2.945ValAsn: 2.945 ± 0.079	1.898ValPro: 1.898 ± 0.06	1.185ValGln: 1.185 ± 0.051	2.291ValArg: 2.291 ± 0.072	3.917ValSer: 3.917 ± 0.096	2.728ValThr: 2.728 ± 0.113	3.755ValVal: 3.755 ± 0.104	0.374ValTrp: 0.374 ± 0.026	2.223ValTyr: 2.223 ± 0.083	0.0ValXaa: 0.0 ± 0.0
Trp	0.459TrpAla: 0.459 ± 0.036	0.042TrpCys: 0.042 ± 0.009	0.414TrpAsp: 0.414 ± 0.029	0.435TrpGlu: 0.435 ± 0.03	0.221TrpPhe: 0.221 ± 0.022	0.444TrpGly: 0.444 ± 0.04	0.1TrpHis: 0.1 ± 0.013	0.539TrpIle: 0.539 ± 0.037	0.51TrpLys: 0.51 ± 0.036	0.51TrpLeu: 0.51 ± 0.034	0.153TrpMet: 0.153 ± 0.019	0.355TrpAsn: 0.355 ± 0.029	0.153TrpPro: 0.153 ± 0.018	0.208TrpGln: 0.208 ± 0.017	0.253TrpArg: 0.253 ± 0.021	0.405TrpSer: 0.405 ± 0.032	0.371TrpThr: 0.371 ± 0.028	0.367TrpVal: 0.367 ± 0.029	0.062TrpTrp: 0.062 ± 0.012	0.284TrpTyr: 0.284 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.104TyrAla: 2.104 ± 0.064	0.405TyrCys: 0.405 ± 0.034	2.601TyrAsp: 2.601 ± 0.083	3.029TyrGlu: 3.029 ± 0.081	2.085TyrPhe: 2.085 ± 0.057	2.543TyrGly: 2.543 ± 0.06	0.497TyrHis: 0.497 ± 0.028	3.267TyrIle: 3.267 ± 0.081	4.136TyrLys: 4.136 ± 0.097	3.787TyrLeu: 3.787 ± 0.112	0.976TyrMet: 0.976 ± 0.042	1.926TyrAsn: 1.926 ± 0.073	1.267TyrPro: 1.267 ± 0.052	0.991TyrGln: 0.991 ± 0.044	1.607TyrArg: 1.607 ± 0.054	2.39TyrSer: 2.39 ± 0.089	1.809TyrThr: 1.809 ± 0.068	2.25TyrVal: 2.25 ± 0.07	0.255TyrTrp: 0.255 ± 0.024	1.811TyrTyr: 1.811 ± 0.064	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1672 proteins (528957 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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