Nitrosomonas sp. (strain Is79A3)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; unclassified Nitrosomonas

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8GLX1|F8GLX1_NITSI Uncharacterized protein OS=Nitrosomonas sp. (strain Is79A3) OX=261292 GN=Nit79A3_3319 PE=4 SV=1
MM1 pKa = 7.13TFHH4 pKa = 6.71VFHH7 pKa = 7.66INEE10 pKa = 4.28VYY12 pKa = 10.95SNANGTVQFIEE23 pKa = 4.53FSGDD27 pKa = 3.81ANDD30 pKa = 3.44QDD32 pKa = 3.74EE33 pKa = 4.75WAGHH37 pKa = 6.59TITSTNGVTTNTYY50 pKa = 8.46TIPTNLPSEE59 pKa = 4.18ATLNKK64 pKa = 8.68TVLVATQGFADD75 pKa = 4.68LGIVTPDD82 pKa = 3.34YY83 pKa = 10.68IIPNGFLFTTSGIVNFPGMVGGKK106 pKa = 7.64ITYY109 pKa = 7.58ATLPVDD115 pKa = 5.1GIKK118 pKa = 10.52SLNPDD123 pKa = 3.06SSSGINSPQNFAGDD137 pKa = 3.72TGTVPSNVFSGTDD150 pKa = 3.47GPDD153 pKa = 3.28NLVGTSGDD161 pKa = 3.96DD162 pKa = 4.07FILGADD168 pKa = 4.08GQDD171 pKa = 3.42TLNGTGGNDD180 pKa = 3.48TLSGGLGVDD189 pKa = 3.26TAIYY193 pKa = 10.24NSNRR197 pKa = 11.84ANYY200 pKa = 9.67SILGTSTGLSISGPDD215 pKa = 3.63GNDD218 pKa = 2.85TVSGIEE224 pKa = 3.89RR225 pKa = 11.84FQFTDD230 pKa = 2.98KK231 pKa = 11.25NIAVDD236 pKa = 4.45LNAGQAASNTVRR248 pKa = 11.84IIGAAFDD255 pKa = 3.91APTIDD260 pKa = 3.45EE261 pKa = 4.6HH262 pKa = 6.88PDD264 pKa = 3.29YY265 pKa = 11.53VGIGLNLFDD274 pKa = 4.77SGQSILEE281 pKa = 4.12VAQLVIGVLGNPSNGAFVDD300 pKa = 3.96KK301 pKa = 10.98VFQNVVGAAPSAADD315 pKa = 3.07HH316 pKa = 6.77DD317 pKa = 5.04FYY319 pKa = 11.88VGLLQGSGGSYY330 pKa = 8.66TQAEE334 pKa = 4.78LLVLAANSDD343 pKa = 3.99VNATNIDD350 pKa = 4.01LVGLQQSGVVFAA362 pKa = 5.6

Molecular weight: 37.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8GHV1|F8GHV1_NITSI Transport permease protein OS=Nitrosomonas sp. (strain Is79A3) OX=261292 GN=Nit79A3_0435 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.43RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MKK23 pKa = 9.13TRR25 pKa = 11.84AGAAVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.96GRR39 pKa = 11.84ARR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3273

0

3273

1023771

31

3212

312.8

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.97 ± 0.047

1.013 ± 0.014

5.315 ± 0.032

6.019 ± 0.042

4.036 ± 0.028

6.836 ± 0.037

2.415 ± 0.021

7.001 ± 0.036

5.136 ± 0.049

10.466 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.02

4.279 ± 0.034

4.276 ± 0.028

4.35 ± 0.031

5.315 ± 0.044

6.22 ± 0.031

5.437 ± 0.041

6.324 ± 0.033

1.301 ± 0.017

2.875 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski