Nerine virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2V0S0|Q2V0S0_9VIRU ORF1 protein OS=Nerine virus X OX=333348 PE=4 SV=1
MM1 pKa = 7.5EE2 pKa = 5.7NIINKK7 pKa = 8.91LQTRR11 pKa = 11.84FTRR14 pKa = 11.84TSLPISKK21 pKa = 9.66PIIIHH26 pKa = 5.93CCAGAGKK33 pKa = 7.33TTFIRR38 pKa = 11.84EE39 pKa = 4.2LLEE42 pKa = 3.95QFPEE46 pKa = 4.35VQAYY50 pKa = 6.81THH52 pKa = 5.73SQHH55 pKa = 6.61LAAEE59 pKa = 4.35KK60 pKa = 9.88TISGRR65 pKa = 11.84NFHH68 pKa = 6.82HH69 pKa = 6.9FSEE72 pKa = 4.55YY73 pKa = 9.67QPGYY77 pKa = 10.42LDD79 pKa = 5.39VIDD82 pKa = 5.35EE83 pKa = 4.35YY84 pKa = 11.62LAGPIPEE91 pKa = 4.28TCHH94 pKa = 6.21FCFADD99 pKa = 4.76PYY101 pKa = 10.75QYY103 pKa = 11.3NIDD106 pKa = 3.98ALPAHH111 pKa = 6.96FICNKK116 pKa = 9.37SYY118 pKa = 11.23RR119 pKa = 11.84FGTLTADD126 pKa = 3.67YY127 pKa = 9.97LNSLGYY133 pKa = 8.14QVRR136 pKa = 11.84SAGGSDD142 pKa = 3.77SIRR145 pKa = 11.84FIDD148 pKa = 3.88LAHH151 pKa = 7.06WEE153 pKa = 4.3PEE155 pKa = 4.83GICITQDD162 pKa = 3.06SDD164 pKa = 3.85ILNLLTRR171 pKa = 11.84HH172 pKa = 6.65GISAYY177 pKa = 10.19HH178 pKa = 6.1PCEE181 pKa = 3.81TLGLQFNCVDD191 pKa = 3.61YY192 pKa = 10.86LVPKK196 pKa = 10.55LPTPATADD204 pKa = 3.34ANTYY208 pKa = 10.17LALTRR213 pKa = 11.84HH214 pKa = 5.53SSRR217 pKa = 11.84LNVVSDD223 pKa = 3.93AADD226 pKa = 3.31SATT229 pKa = 3.56

Molecular weight: 25.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2V0R9|Q2V0R9_9VIRU Triple geneblock protein 1 OS=Nerine virus X OX=333348 PE=4 SV=1
MM1 pKa = 7.91PLTAPPDD8 pKa = 3.67YY9 pKa = 10.53THH11 pKa = 7.51ILPIAIVSIAVALSLYY27 pKa = 9.97TITRR31 pKa = 11.84NTLPHH36 pKa = 6.23TGDD39 pKa = 4.17NIHH42 pKa = 6.69HH43 pKa = 6.66FPHH46 pKa = 6.73GGRR49 pKa = 11.84YY50 pKa = 8.78RR51 pKa = 11.84DD52 pKa = 3.66GTKK55 pKa = 10.39SITYY59 pKa = 9.13CPPQRR64 pKa = 11.84NLSPFNSTGYY74 pKa = 10.48SIIPTALAILLPAAIYY90 pKa = 10.46LSSKK94 pKa = 9.81CFNSRR99 pKa = 11.84THH101 pKa = 6.05PHH103 pKa = 6.03SCSHH107 pKa = 6.31CQPNSATMRR116 pKa = 11.84GTSS119 pKa = 3.03

Molecular weight: 12.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2193

119

1504

438.6

49.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.664 ± 1.74

1.87 ± 0.499

4.514 ± 0.583

5.928 ± 1.336

3.694 ± 0.47

4.423 ± 0.225

4.15 ± 0.564

6.065 ± 0.76

5.016 ± 1.123

9.166 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.006 ± 0.387

4.606 ± 0.121

7.342 ± 0.729

4.514 ± 0.487

4.514 ± 0.271

7.068 ± 1.039

7.478 ± 0.568

4.651 ± 0.676

1.003 ± 0.258

3.329 ± 0.519
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski