Cyanophage S-TIM4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Thaumasvirus; Synechococcus virus STIM4

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 235 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AWT1|A0A345AWT1_9CAUD Uncharacterized protein OS=Cyanophage S-TIM4 OX=1048189 GN=ORF_228 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 5.01IALVVLTTIGAFILGITVSWLAKK25 pKa = 10.34GYY27 pKa = 10.55VEE29 pKa = 6.23DD30 pKa = 4.57YY31 pKa = 10.93IEE33 pKa = 4.03NAAYY37 pKa = 9.89AKK39 pKa = 10.38SVTHH43 pKa = 6.92PEE45 pKa = 4.05MFDD48 pKa = 3.25EE49 pKa = 5.33DD50 pKa = 3.88GDD52 pKa = 4.28MIHH55 pKa = 7.67DD56 pKa = 3.76EE57 pKa = 4.97LIYY60 pKa = 10.32IRR62 pKa = 11.84PDD64 pKa = 3.16YY65 pKa = 10.9RR66 pKa = 11.84FLQHH70 pKa = 7.06EE71 pKa = 5.11DD72 pKa = 4.49DD73 pKa = 6.27DD74 pKa = 7.15DD75 pKa = 7.3DD76 pKa = 7.1DD77 pKa = 4.67DD78 pKa = 4.3

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AWH3|A0A345AWH3_9CAUD Uncharacterized protein OS=Cyanophage S-TIM4 OX=1048189 GN=ORF_120 PE=4 SV=1
MM1 pKa = 7.37FFASHH6 pKa = 6.79PSVYY10 pKa = 9.52TLPGTWEE17 pKa = 3.87PQPDD21 pKa = 3.78VLYY24 pKa = 11.14NPTLLISLGSTAFAIGAIVSILAVRR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 9.61RR52 pKa = 11.84KK53 pKa = 9.87RR54 pKa = 11.84II55 pKa = 3.51

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

235

0

235

56598

26

6603

240.8

26.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.002 ± 0.222

0.875 ± 0.09

6.587 ± 0.125

5.861 ± 0.319

4.339 ± 0.113

7.774 ± 0.342

1.615 ± 0.124

6.244 ± 0.172

6.207 ± 0.429

7.122 ± 0.155

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.039 ± 0.203

6.0 ± 0.183

3.884 ± 0.14

3.587 ± 0.093

3.921 ± 0.177

6.945 ± 0.222

7.937 ± 0.529

6.631 ± 0.203

1.124 ± 0.085

4.308 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski