Streptococcus phage Javan214

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AVC0|A0A4D6AVC0_9CAUD Portal protein OS=Streptococcus phage Javan214 OX=2548050 GN=Javan214_0047 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.39KK3 pKa = 10.11LALSTKK9 pKa = 9.68YY10 pKa = 10.74INDD13 pKa = 3.68RR14 pKa = 11.84EE15 pKa = 3.94FWAYY19 pKa = 10.63GNWSDD24 pKa = 3.55STHH27 pKa = 6.33FRR29 pKa = 11.84GTEE32 pKa = 3.84CKK34 pKa = 9.75QGYY37 pKa = 7.51FLCSSWEE44 pKa = 4.08EE45 pKa = 4.03LWSLLYY51 pKa = 10.35YY52 pKa = 11.16VNGSNDD58 pKa = 3.13TAQYY62 pKa = 11.37NEE64 pKa = 4.1VEE66 pKa = 4.07DD67 pKa = 4.18TYY69 pKa = 10.97IYY71 pKa = 10.52SQGDD75 pKa = 3.3RR76 pKa = 11.84FDD78 pKa = 4.61GLEE81 pKa = 4.07LDD83 pKa = 5.79DD84 pKa = 3.92IVEE87 pKa = 4.72MYY89 pKa = 10.51TEE91 pKa = 5.07DD92 pKa = 5.7DD93 pKa = 3.9YY94 pKa = 12.15QEE96 pKa = 4.81LCDD99 pKa = 4.33NFMGNDD105 pKa = 3.17SVNRR109 pKa = 11.84HH110 pKa = 5.39IFFEE114 pKa = 4.26LTDD117 pKa = 3.93DD118 pKa = 4.69DD119 pKa = 5.42FRR121 pKa = 11.84ILKK124 pKa = 10.29DD125 pKa = 3.28DD126 pKa = 5.05LKK128 pKa = 11.09RR129 pKa = 11.84GWEE132 pKa = 3.83PDD134 pKa = 3.03LLQFINSHH142 pKa = 5.78KK143 pKa = 10.35EE144 pKa = 3.63RR145 pKa = 11.84EE146 pKa = 3.95NDD148 pKa = 3.04ILEE151 pKa = 4.31WVLLDD156 pKa = 4.37AEE158 pKa = 4.92NYY160 pKa = 7.31TINYY164 pKa = 9.27SFDD167 pKa = 3.31NEE169 pKa = 4.12RR170 pKa = 11.84CKK172 pKa = 11.11DD173 pKa = 3.96DD174 pKa = 3.7LCIWEE179 pKa = 4.5DD180 pKa = 3.24

Molecular weight: 21.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AUY8|A0A4D6AUY8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan214 OX=2548050 GN=Javan214_0052 PE=4 SV=1
MM1 pKa = 7.41QSRR4 pKa = 11.84KK5 pKa = 7.34TKK7 pKa = 10.34RR8 pKa = 11.84KK9 pKa = 9.45EE10 pKa = 3.76KK11 pKa = 9.75TLSNINDD18 pKa = 3.59TTKK21 pKa = 11.0LVIAVTSLVKK31 pKa = 10.68AIIKK35 pKa = 9.18LLKK38 pKa = 10.67VFFF41 pKa = 4.99

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12414

38

1498

185.3

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.597 ± 0.653

0.693 ± 0.125

6.815 ± 0.231

7.838 ± 0.539

4.334 ± 0.319

6.348 ± 0.425

1.176 ± 0.124

6.146 ± 0.312

8.7 ± 0.394

8.176 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.208

5.921 ± 0.264

2.505 ± 0.122

3.577 ± 0.209

3.818 ± 0.258

6.227 ± 0.327

6.589 ± 0.493

6.332 ± 0.247

1.377 ± 0.153

4.14 ± 0.315
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski