Dufourea novaeangliae (Sweat bee)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Halictidae;

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11355 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A154PN07|A0A154PN07_DUFNO DUF4758 domain-containing protein OS=Dufourea novaeangliae OX=178035 GN=WN55_05931 PE=4 SV=1
MM1 pKa = 7.32MNFEE5 pKa = 4.67GEE7 pKa = 4.24QGSMRR12 pKa = 11.84SSSSRR17 pKa = 11.84NIICCLATARR27 pKa = 11.84YY28 pKa = 9.73NNISQAIQFWVYY40 pKa = 10.89SQLKK44 pKa = 8.94KK45 pKa = 10.6ARR47 pKa = 11.84NMDD50 pKa = 4.07DD51 pKa = 4.79LQDD54 pKa = 3.74QSDD57 pKa = 3.81HH58 pKa = 6.38GLIYY62 pKa = 10.47CRR64 pKa = 11.84YY65 pKa = 10.46DD66 pKa = 2.92NSTNNNTLMEE76 pKa = 4.19YY77 pKa = 10.94LEE79 pKa = 4.66LDD81 pKa = 3.5NRR83 pKa = 11.84TSDD86 pKa = 3.71PLNEE90 pKa = 4.66PEE92 pKa = 3.9VTMNLNEE99 pKa = 4.45GSVQLLLYY107 pKa = 9.62PLEE110 pKa = 4.22EE111 pKa = 4.88LEE113 pKa = 5.07EE114 pKa = 4.64DD115 pKa = 3.39MFDD118 pKa = 4.1YY119 pKa = 9.34EE120 pKa = 4.99TDD122 pKa = 3.23TSSQDD127 pKa = 3.33GNIEE131 pKa = 3.95SQEE134 pKa = 3.88QVVPSSQLSNNHH146 pKa = 6.75KK147 pKa = 10.57YY148 pKa = 10.34PEE150 pKa = 4.34EE151 pKa = 3.83LTISDD156 pKa = 5.38DD157 pKa = 4.35EE158 pKa = 5.58IDD160 pKa = 4.59DD161 pKa = 4.28GGTFEE166 pKa = 6.06DD167 pKa = 4.54EE168 pKa = 4.29PVSNLLLNDD177 pKa = 3.62NDD179 pKa = 4.58TDD181 pKa = 4.53DD182 pKa = 4.13SQPPP186 pKa = 3.32

Molecular weight: 21.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A154PQN9|A0A154PQN9_DUFNO 5-hydroxytryptamine receptor 2B OS=Dufourea novaeangliae OX=178035 GN=WN55_05217 PE=3 SV=1
MM1 pKa = 7.03HH2 pKa = 7.52RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84VRR7 pKa = 11.84RR8 pKa = 11.84IPTARR13 pKa = 11.84AAAFSRR19 pKa = 11.84QKK21 pKa = 10.65RR22 pKa = 11.84GGPSRR27 pKa = 11.84WWWKK31 pKa = 8.96YY32 pKa = 8.08KK33 pKa = 10.2ARR35 pKa = 11.84GVAGHH40 pKa = 5.72SQSVSGTPTPSRR52 pKa = 11.84SASLKK57 pKa = 6.97PTPII61 pKa = 4.53

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11355

0

11355

5528599

29

18781

486.9

54.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.063 ± 0.024

2.007 ± 0.046

5.426 ± 0.019

6.879 ± 0.044

3.479 ± 0.017

5.676 ± 0.031

2.498 ± 0.015

5.544 ± 0.024

6.358 ± 0.038

8.924 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.241 ± 0.011

4.98 ± 0.025

5.188 ± 0.033

4.248 ± 0.025

5.789 ± 0.025

8.082 ± 0.031

6.142 ± 0.028

6.281 ± 0.022

1.097 ± 0.009

3.087 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski