Paracoccus tibetensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3733 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5HMT8|A0A1G5HMT8_9RHOB GTPase G3E family OS=Paracoccus tibetensis OX=336292 GN=SAMN05660710_02198 PE=3 SV=1
MM1 pKa = 7.85LEE3 pKa = 3.98RR4 pKa = 11.84LALATAVAAFPLMAYY19 pKa = 9.6AQTAEE24 pKa = 4.46TEE26 pKa = 4.35TPPDD30 pKa = 3.14AEE32 pKa = 4.43AAVEE36 pKa = 4.39TPVEE40 pKa = 4.07EE41 pKa = 4.47TPAADD46 pKa = 3.65AAATEE51 pKa = 4.21ATTDD55 pKa = 4.01DD56 pKa = 3.83VATDD60 pKa = 3.78DD61 pKa = 4.63EE62 pKa = 4.49ATEE65 pKa = 5.02DD66 pKa = 3.56EE67 pKa = 4.75AAPADD72 pKa = 4.25GSDD75 pKa = 3.13EE76 pKa = 3.97AAAEE80 pKa = 4.2GEE82 pKa = 4.31PAAEE86 pKa = 4.27AAAEE90 pKa = 4.23DD91 pKa = 4.08ADD93 pKa = 4.29AGAFTLPEE101 pKa = 4.13LGYY104 pKa = 10.71AYY106 pKa = 10.1DD107 pKa = 3.77ALEE110 pKa = 4.15PVIDD114 pKa = 4.01AQTMEE119 pKa = 4.3LHH121 pKa = 6.69HH122 pKa = 6.6SRR124 pKa = 11.84HH125 pKa = 5.7HH126 pKa = 4.77QTFVDD131 pKa = 3.75NLNDD135 pKa = 3.56AVEE138 pKa = 4.41NGVIPADD145 pKa = 3.56IEE147 pKa = 4.0IEE149 pKa = 4.32EE150 pKa = 4.33ILASASTYY158 pKa = 9.89PNQVRR163 pKa = 11.84NSAGGHH169 pKa = 4.73WNHH172 pKa = 5.66TFFWEE177 pKa = 3.77IMAPEE182 pKa = 4.7GEE184 pKa = 4.2RR185 pKa = 11.84GEE187 pKa = 4.22MSDD190 pKa = 4.25ALSEE194 pKa = 4.94AITAVYY200 pKa = 10.11GSEE203 pKa = 4.17EE204 pKa = 4.02EE205 pKa = 4.46FRR207 pKa = 11.84TAFNDD212 pKa = 2.97AGAARR217 pKa = 11.84FGSGWVWLIVNDD229 pKa = 4.12ANQLEE234 pKa = 4.88ITTTPNQDD242 pKa = 3.41NPLMDD247 pKa = 4.26AAEE250 pKa = 4.23VQGTPIIGNDD260 pKa = 3.27VWEE263 pKa = 4.08HH264 pKa = 6.11AYY266 pKa = 10.28YY267 pKa = 11.05LNYY270 pKa = 9.41QNRR273 pKa = 11.84RR274 pKa = 11.84AEE276 pKa = 4.0YY277 pKa = 9.4LQNWWDD283 pKa = 3.57VVNWDD288 pKa = 3.99EE289 pKa = 4.47VSARR293 pKa = 11.84YY294 pKa = 7.96EE295 pKa = 3.75AALAEE300 pKa = 4.3

Molecular weight: 32.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5J786|A0A1G5J786_9RHOB 30S ribosomal protein S14 OS=Paracoccus tibetensis OX=336292 GN=rpsN PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.39GGRR28 pKa = 11.84LVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3733

0

3733

1171507

18

2132

313.8

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.857 ± 0.069

0.808 ± 0.013

5.617 ± 0.032

5.761 ± 0.038

3.423 ± 0.025

8.89 ± 0.042

2.039 ± 0.022

4.94 ± 0.032

2.247 ± 0.031

10.636 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.74 ± 0.019

2.191 ± 0.021

5.609 ± 0.036

3.286 ± 0.027

7.681 ± 0.047

4.776 ± 0.027

5.098 ± 0.025

7.022 ± 0.034

1.458 ± 0.016

1.92 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski