Amino acid dipepetide frequency for Gordonia phage Huffy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.085AlaAla: 17.085 ± 1.579
0.851AlaCys: 0.851 ± 0.26
8.365AlaAsp: 8.365 ± 0.793
9.287AlaGlu: 9.287 ± 1.098
3.757AlaPhe: 3.757 ± 0.652
14.816AlaGly: 14.816 ± 1.634
2.339AlaHis: 2.339 ± 0.485
4.112AlaIle: 4.112 ± 0.591
6.238AlaLys: 6.238 ± 0.828
9.712AlaLeu: 9.712 ± 0.84
2.836AlaMet: 2.836 ± 0.521
3.474AlaAsn: 3.474 ± 0.517
4.324AlaPro: 4.324 ± 0.669
3.615AlaGln: 3.615 ± 0.495
9.145AlaArg: 9.145 ± 0.893
5.742AlaSer: 5.742 ± 0.729
6.947AlaThr: 6.947 ± 0.665
8.365AlaVal: 8.365 ± 1.028
2.127AlaTrp: 2.127 ± 0.338
2.907AlaTyr: 2.907 ± 0.415
0.0AlaXaa: 0.0 ± 0.0
Cys
0.78CysAla: 0.78 ± 0.235
0.071CysCys: 0.071 ± 0.083
0.567CysAsp: 0.567 ± 0.178
0.496CysGlu: 0.496 ± 0.207
0.071CysPhe: 0.071 ± 0.069
1.347CysGly: 1.347 ± 0.34
0.284CysHis: 0.284 ± 0.144
0.284CysIle: 0.284 ± 0.138
0.354CysLys: 0.354 ± 0.134
0.354CysLeu: 0.354 ± 0.149
0.142CysMet: 0.142 ± 0.098
0.354CysAsn: 0.354 ± 0.146
0.354CysPro: 0.354 ± 0.189
0.213CysGln: 0.213 ± 0.12
0.709CysArg: 0.709 ± 0.227
0.567CysSer: 0.567 ± 0.176
0.071CysThr: 0.071 ± 0.083
0.496CysVal: 0.496 ± 0.191
0.284CysTrp: 0.284 ± 0.132
0.213CysTyr: 0.213 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
9.145AspAla: 9.145 ± 0.841
0.922AspCys: 0.922 ± 0.218
4.537AspAsp: 4.537 ± 0.538
6.451AspGlu: 6.451 ± 0.853
1.772AspPhe: 1.772 ± 0.343
6.238AspGly: 6.238 ± 0.685
1.276AspHis: 1.276 ± 0.34
1.205AspIle: 1.205 ± 0.222
1.56AspLys: 1.56 ± 0.345
5.175AspLeu: 5.175 ± 0.791
1.418AspMet: 1.418 ± 0.306
1.063AspAsn: 1.063 ± 0.211
4.254AspPro: 4.254 ± 0.496
3.119AspGln: 3.119 ± 0.427
6.168AspArg: 6.168 ± 0.673
3.615AspSer: 3.615 ± 0.496
2.907AspThr: 2.907 ± 0.42
5.955AspVal: 5.955 ± 0.61
0.992AspTrp: 0.992 ± 0.234
1.489AspTyr: 1.489 ± 0.321
0.0AspXaa: 0.0 ± 0.0
Glu
8.082GluAla: 8.082 ± 0.77
0.567GluCys: 0.567 ± 0.238
3.97GluAsp: 3.97 ± 0.683
2.481GluGlu: 2.481 ± 0.378
2.339GluPhe: 2.339 ± 0.449
5.459GluGly: 5.459 ± 0.59
1.772GluHis: 1.772 ± 0.375
1.489GluIle: 1.489 ± 0.343
1.843GluLys: 1.843 ± 0.3
6.806GluLeu: 6.806 ± 0.815
1.489GluMet: 1.489 ± 0.329
1.631GluAsn: 1.631 ± 0.39
3.048GluPro: 3.048 ± 0.496
2.41GluGln: 2.41 ± 0.465
5.671GluArg: 5.671 ± 0.729
4.041GluSer: 4.041 ± 0.736
3.403GluThr: 3.403 ± 0.577
5.742GluVal: 5.742 ± 0.833
1.843GluTrp: 1.843 ± 0.348
1.914GluTyr: 1.914 ± 0.349
0.0GluXaa: 0.0 ± 0.0
Phe
4.183PheAla: 4.183 ± 0.617
0.213PheCys: 0.213 ± 0.114
1.56PheAsp: 1.56 ± 0.302
3.048PheGlu: 3.048 ± 0.46
0.567PhePhe: 0.567 ± 0.21
2.552PheGly: 2.552 ± 0.475
0.567PheHis: 0.567 ± 0.18
0.78PheIle: 0.78 ± 0.226
0.922PheLys: 0.922 ± 0.27
1.631PheLeu: 1.631 ± 0.322
0.992PheMet: 0.992 ± 0.266
0.496PheAsn: 0.496 ± 0.155
0.78PhePro: 0.78 ± 0.287
1.063PheGln: 1.063 ± 0.263
2.765PheArg: 2.765 ± 0.424
1.701PheSer: 1.701 ± 0.304
2.552PheThr: 2.552 ± 0.442
2.127PheVal: 2.127 ± 0.454
0.425PheTrp: 0.425 ± 0.157
0.425PheTyr: 0.425 ± 0.199
0.0PheXaa: 0.0 ± 0.0
Gly
9.287GlyAla: 9.287 ± 1.247
0.567GlyCys: 0.567 ± 0.213
7.869GlyAsp: 7.869 ± 0.655
5.955GlyGlu: 5.955 ± 0.402
2.977GlyPhe: 2.977 ± 0.535
10.988GlyGly: 10.988 ± 1.277
1.701GlyHis: 1.701 ± 0.389
3.403GlyIle: 3.403 ± 0.501
4.608GlyLys: 4.608 ± 0.623
5.671GlyLeu: 5.671 ± 0.719
2.977GlyMet: 2.977 ± 0.428
2.694GlyAsn: 2.694 ± 0.483
4.395GlyPro: 4.395 ± 0.842
3.545GlyGln: 3.545 ± 0.536
6.877GlyArg: 6.877 ± 0.536
5.246GlySer: 5.246 ± 0.581
4.821GlyThr: 4.821 ± 0.519
7.16GlyVal: 7.16 ± 1.066
2.056GlyTrp: 2.056 ± 0.498
2.481GlyTyr: 2.481 ± 0.479
0.0GlyXaa: 0.0 ± 0.0
His
2.481HisAla: 2.481 ± 0.499
0.142HisCys: 0.142 ± 0.104
1.347HisAsp: 1.347 ± 0.407
1.063HisGlu: 1.063 ± 0.316
0.567HisPhe: 0.567 ± 0.177
1.985HisGly: 1.985 ± 0.427
0.425HisHis: 0.425 ± 0.179
0.567HisIle: 0.567 ± 0.234
0.638HisLys: 0.638 ± 0.214
1.276HisLeu: 1.276 ± 0.281
0.284HisMet: 0.284 ± 0.124
0.567HisAsn: 0.567 ± 0.179
0.922HisPro: 0.922 ± 0.287
0.638HisGln: 0.638 ± 0.244
1.205HisArg: 1.205 ± 0.284
0.851HisSer: 0.851 ± 0.226
0.992HisThr: 0.992 ± 0.243
2.269HisVal: 2.269 ± 0.468
0.638HisTrp: 0.638 ± 0.224
0.496HisTyr: 0.496 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
5.671IleAla: 5.671 ± 0.715
0.213IleCys: 0.213 ± 0.137
2.339IleAsp: 2.339 ± 0.389
3.545IleGlu: 3.545 ± 0.615
0.567IlePhe: 0.567 ± 0.18
3.19IleGly: 3.19 ± 0.499
0.567IleHis: 0.567 ± 0.207
0.992IleIle: 0.992 ± 0.227
0.354IleLys: 0.354 ± 0.139
2.127IleLeu: 2.127 ± 0.387
0.709IleMet: 0.709 ± 0.214
0.354IleAsn: 0.354 ± 0.133
2.765IlePro: 2.765 ± 0.402
1.276IleGln: 1.276 ± 0.314
1.914IleArg: 1.914 ± 0.433
1.56IleSer: 1.56 ± 0.352
2.056IleThr: 2.056 ± 0.372
2.552IleVal: 2.552 ± 0.431
0.284IleTrp: 0.284 ± 0.165
0.567IleTyr: 0.567 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
4.821LysAla: 4.821 ± 0.643
0.354LysCys: 0.354 ± 0.138
2.056LysAsp: 2.056 ± 0.387
0.922LysGlu: 0.922 ± 0.331
1.205LysPhe: 1.205 ± 0.353
3.474LysGly: 3.474 ± 0.401
0.567LysHis: 0.567 ± 0.183
0.851LysIle: 0.851 ± 0.245
0.567LysLys: 0.567 ± 0.241
3.19LysLeu: 3.19 ± 0.427
0.496LysMet: 0.496 ± 0.172
0.992LysAsn: 0.992 ± 0.297
2.269LysPro: 2.269 ± 0.425
0.78LysGln: 0.78 ± 0.257
4.183LysArg: 4.183 ± 0.597
2.269LysSer: 2.269 ± 0.418
1.56LysThr: 1.56 ± 0.359
3.261LysVal: 3.261 ± 0.474
0.567LysTrp: 0.567 ± 0.244
0.922LysTyr: 0.922 ± 0.293
0.0LysXaa: 0.0 ± 0.0
Leu
10.846LeuAla: 10.846 ± 0.898
0.922LeuCys: 0.922 ± 0.251
6.522LeuAsp: 6.522 ± 0.801
3.474LeuGlu: 3.474 ± 0.561
1.914LeuPhe: 1.914 ± 0.36
6.593LeuGly: 6.593 ± 0.773
1.347LeuHis: 1.347 ± 0.315
2.623LeuIle: 2.623 ± 0.309
2.907LeuLys: 2.907 ± 0.553
4.183LeuLeu: 4.183 ± 0.614
1.843LeuMet: 1.843 ± 0.367
1.914LeuAsn: 1.914 ± 0.536
3.757LeuPro: 3.757 ± 0.58
1.418LeuGln: 1.418 ± 0.307
5.955LeuArg: 5.955 ± 0.776
5.033LeuSer: 5.033 ± 0.592
4.962LeuThr: 4.962 ± 0.538
4.821LeuVal: 4.821 ± 0.532
1.489LeuTrp: 1.489 ± 0.34
2.41LeuTyr: 2.41 ± 0.441
0.0LeuXaa: 0.0 ± 0.0
Met
3.048MetAla: 3.048 ± 0.567
0.142MetCys: 0.142 ± 0.086
1.347MetAsp: 1.347 ± 0.331
0.78MetGlu: 0.78 ± 0.196
0.567MetPhe: 0.567 ± 0.178
1.56MetGly: 1.56 ± 0.298
0.425MetHis: 0.425 ± 0.161
0.922MetIle: 0.922 ± 0.294
0.851MetLys: 0.851 ± 0.303
1.56MetLeu: 1.56 ± 0.394
0.496MetMet: 0.496 ± 0.166
1.347MetAsn: 1.347 ± 0.324
0.922MetPro: 0.922 ± 0.247
0.638MetGln: 0.638 ± 0.205
1.914MetArg: 1.914 ± 0.336
1.701MetSer: 1.701 ± 0.336
1.631MetThr: 1.631 ± 0.39
1.914MetVal: 1.914 ± 0.374
0.78MetTrp: 0.78 ± 0.216
0.425MetTyr: 0.425 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
2.694AsnAla: 2.694 ± 0.448
0.071AsnCys: 0.071 ± 0.071
1.56AsnAsp: 1.56 ± 0.358
1.701AsnGlu: 1.701 ± 0.288
0.992AsnPhe: 0.992 ± 0.27
3.757AsnGly: 3.757 ± 0.514
0.638AsnHis: 0.638 ± 0.271
0.922AsnIle: 0.922 ± 0.341
0.638AsnLys: 0.638 ± 0.213
1.914AsnLeu: 1.914 ± 0.441
0.638AsnMet: 0.638 ± 0.178
0.354AsnAsn: 0.354 ± 0.194
2.694AsnPro: 2.694 ± 0.463
0.851AsnGln: 0.851 ± 0.241
1.985AsnArg: 1.985 ± 0.34
1.205AsnSer: 1.205 ± 0.324
1.701AsnThr: 1.701 ± 0.46
2.339AsnVal: 2.339 ± 0.335
0.284AsnTrp: 0.284 ± 0.138
0.638AsnTyr: 0.638 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
7.018ProAla: 7.018 ± 0.858
0.496ProCys: 0.496 ± 0.185
3.828ProAsp: 3.828 ± 0.589
4.041ProGlu: 4.041 ± 0.529
1.489ProPhe: 1.489 ± 0.343
4.112ProGly: 4.112 ± 0.504
1.205ProHis: 1.205 ± 0.291
2.552ProIle: 2.552 ± 0.422
1.56ProLys: 1.56 ± 0.359
2.836ProLeu: 2.836 ± 0.517
1.063ProMet: 1.063 ± 0.278
1.134ProAsn: 1.134 ± 0.337
1.631ProPro: 1.631 ± 0.393
1.418ProGln: 1.418 ± 0.34
2.765ProArg: 2.765 ± 0.494
1.631ProSer: 1.631 ± 0.338
2.339ProThr: 2.339 ± 0.397
4.254ProVal: 4.254 ± 0.471
1.134ProTrp: 1.134 ± 0.284
1.134ProTyr: 1.134 ± 0.249
0.0ProXaa: 0.0 ± 0.0
Gln
3.403GlnAla: 3.403 ± 0.49
0.071GlnCys: 0.071 ± 0.085
1.489GlnAsp: 1.489 ± 0.386
0.78GlnGlu: 0.78 ± 0.21
0.851GlnPhe: 0.851 ± 0.248
2.198GlnGly: 2.198 ± 0.355
0.496GlnHis: 0.496 ± 0.166
0.992GlnIle: 0.992 ± 0.255
0.78GlnLys: 0.78 ± 0.278
3.403GlnLeu: 3.403 ± 0.449
0.638GlnMet: 0.638 ± 0.215
0.992GlnAsn: 0.992 ± 0.221
1.631GlnPro: 1.631 ± 0.313
1.347GlnGln: 1.347 ± 0.356
3.19GlnArg: 3.19 ± 0.533
2.056GlnSer: 2.056 ± 0.387
1.985GlnThr: 1.985 ± 0.457
2.269GlnVal: 2.269 ± 0.387
0.78GlnTrp: 0.78 ± 0.215
0.922GlnTyr: 0.922 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
10.067ArgAla: 10.067 ± 0.845
0.638ArgCys: 0.638 ± 0.217
5.317ArgAsp: 5.317 ± 0.677
5.884ArgGlu: 5.884 ± 0.711
2.907ArgPhe: 2.907 ± 0.34
6.309ArgGly: 6.309 ± 0.869
1.701ArgHis: 1.701 ± 0.406
3.686ArgIle: 3.686 ± 0.395
3.19ArgLys: 3.19 ± 0.432
5.459ArgLeu: 5.459 ± 0.727
1.843ArgMet: 1.843 ± 0.334
2.552ArgAsn: 2.552 ± 0.378
3.332ArgPro: 3.332 ± 0.509
1.56ArgGln: 1.56 ± 0.252
7.585ArgArg: 7.585 ± 0.842
2.977ArgSer: 2.977 ± 0.489
4.395ArgThr: 4.395 ± 0.462
6.593ArgVal: 6.593 ± 0.883
1.134ArgTrp: 1.134 ± 0.309
2.339ArgTyr: 2.339 ± 0.407
0.0ArgXaa: 0.0 ± 0.0
Ser
7.373SerAla: 7.373 ± 0.966
0.496SerCys: 0.496 ± 0.198
4.75SerAsp: 4.75 ± 0.67
3.19SerGlu: 3.19 ± 0.498
1.134SerPhe: 1.134 ± 0.25
5.246SerGly: 5.246 ± 0.714
0.638SerHis: 0.638 ± 0.204
1.914SerIle: 1.914 ± 0.318
1.418SerLys: 1.418 ± 0.323
4.254SerLeu: 4.254 ± 0.53
1.063SerMet: 1.063 ± 0.24
1.701SerAsn: 1.701 ± 0.319
1.914SerPro: 1.914 ± 0.335
1.276SerGln: 1.276 ± 0.239
3.332SerArg: 3.332 ± 0.454
2.907SerSer: 2.907 ± 0.462
2.623SerThr: 2.623 ± 0.448
4.395SerVal: 4.395 ± 0.554
1.347SerTrp: 1.347 ± 0.414
0.992SerTyr: 0.992 ± 0.299
0.0SerXaa: 0.0 ± 0.0
Thr
6.168ThrAla: 6.168 ± 0.678
0.425ThrCys: 0.425 ± 0.217
3.474ThrAsp: 3.474 ± 0.54
3.332ThrGlu: 3.332 ± 0.529
2.056ThrPhe: 2.056 ± 0.387
4.395ThrGly: 4.395 ± 0.562
0.922ThrHis: 0.922 ± 0.287
2.694ThrIle: 2.694 ± 0.475
1.56ThrLys: 1.56 ± 0.279
4.537ThrLeu: 4.537 ± 0.486
1.134ThrMet: 1.134 ± 0.312
2.056ThrAsn: 2.056 ± 0.491
3.403ThrPro: 3.403 ± 0.486
1.063ThrGln: 1.063 ± 0.268
3.686ThrArg: 3.686 ± 0.474
2.056ThrSer: 2.056 ± 0.433
3.615ThrThr: 3.615 ± 0.791
6.38ThrVal: 6.38 ± 0.616
1.347ThrTrp: 1.347 ± 0.3
1.772ThrTyr: 1.772 ± 0.403
0.0ThrXaa: 0.0 ± 0.0
Val
9.783ValAla: 9.783 ± 0.722
0.425ValCys: 0.425 ± 0.208
5.246ValAsp: 5.246 ± 0.66
6.522ValGlu: 6.522 ± 0.718
2.127ValPhe: 2.127 ± 0.465
6.38ValGly: 6.38 ± 0.684
1.631ValHis: 1.631 ± 0.376
2.623ValIle: 2.623 ± 0.408
3.686ValLys: 3.686 ± 0.736
6.735ValLeu: 6.735 ± 0.749
1.914ValMet: 1.914 ± 0.295
2.41ValAsn: 2.41 ± 0.381
3.686ValPro: 3.686 ± 0.463
2.198ValGln: 2.198 ± 0.382
5.6ValArg: 5.6 ± 0.611
4.324ValSer: 4.324 ± 0.493
5.175ValThr: 5.175 ± 0.662
7.373ValVal: 7.373 ± 0.836
1.843ValTrp: 1.843 ± 0.3
1.914ValTyr: 1.914 ± 0.395
0.0ValXaa: 0.0 ± 0.0
Trp
1.772TrpAla: 1.772 ± 0.329
0.142TrpCys: 0.142 ± 0.1
1.56TrpAsp: 1.56 ± 0.354
1.418TrpGlu: 1.418 ± 0.297
0.496TrpPhe: 0.496 ± 0.152
1.631TrpGly: 1.631 ± 0.339
0.425TrpHis: 0.425 ± 0.175
0.567TrpIle: 0.567 ± 0.181
0.851TrpLys: 0.851 ± 0.263
2.339TrpLeu: 2.339 ± 0.322
0.284TrpMet: 0.284 ± 0.132
0.638TrpAsn: 0.638 ± 0.234
0.78TrpPro: 0.78 ± 0.219
0.922TrpGln: 0.922 ± 0.245
1.985TrpArg: 1.985 ± 0.445
1.205TrpSer: 1.205 ± 0.273
1.631TrpThr: 1.631 ± 0.313
0.922TrpVal: 0.922 ± 0.256
0.213TrpTrp: 0.213 ± 0.113
0.496TrpTyr: 0.496 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.41TyrAla: 2.41 ± 0.471
0.284TyrCys: 0.284 ± 0.139
1.56TyrAsp: 1.56 ± 0.37
1.631TyrGlu: 1.631 ± 0.332
0.992TyrPhe: 0.992 ± 0.216
3.048TyrGly: 3.048 ± 0.418
0.425TyrHis: 0.425 ± 0.149
0.425TyrIle: 0.425 ± 0.173
0.78TyrLys: 0.78 ± 0.271
1.772TyrLeu: 1.772 ± 0.346
0.638TyrMet: 0.638 ± 0.215
0.851TyrAsn: 0.851 ± 0.201
0.78TyrPro: 0.78 ± 0.229
0.922TyrGln: 0.922 ± 0.206
2.836TyrArg: 2.836 ± 0.56
1.276TyrSer: 1.276 ± 0.318
0.709TyrThr: 0.709 ± 0.198
2.41TyrVal: 2.41 ± 0.427
0.709TyrTrp: 0.709 ± 0.245
0.567TyrTyr: 0.567 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (14107 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski