Thermus phage TSP4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Oshimavirus; unclassified Oshimavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411CW89|A0A411CW89_9CAUD Uncharacterized protein OS=Thermus phage TSP4 OX=1298530 PE=4 SV=1
MM1 pKa = 6.59VQNTGWITCNMCGNPATLHH20 pKa = 5.53EE21 pKa = 4.63VVDD24 pKa = 4.27GTMLSSGHH32 pKa = 5.47ITCPSCGLYY41 pKa = 10.57VSWEE45 pKa = 4.07HH46 pKa = 6.59DD47 pKa = 3.69TVIHH51 pKa = 7.08AEE53 pKa = 4.22YY54 pKa = 10.33KK55 pKa = 11.03DD56 pKa = 4.16PDD58 pKa = 4.33PEE60 pKa = 4.13LADD63 pKa = 3.67YY64 pKa = 10.99LKK66 pKa = 10.5EE67 pKa = 4.06LSAKK71 pKa = 9.52EE72 pKa = 3.84PPAGEE77 pKa = 3.85FHH79 pKa = 6.87EE80 pKa = 5.16EE81 pKa = 3.76FWSDD85 pKa = 3.29VAEE88 pKa = 5.96LLDD91 pKa = 3.28AWYY94 pKa = 10.5ADD96 pKa = 3.33WYY98 pKa = 10.42KK99 pKa = 10.93AVSHH103 pKa = 6.46PTLITYY109 pKa = 10.2EE110 pKa = 4.22EE111 pKa = 4.21DD112 pKa = 3.32TGDD115 pKa = 3.57KK116 pKa = 10.7LILIRR121 pKa = 11.84NVPAWFGPEE130 pKa = 3.56YY131 pKa = 10.29EE132 pKa = 4.57IYY134 pKa = 11.01YY135 pKa = 9.87DD136 pKa = 3.59AQVADD141 pKa = 4.53KK142 pKa = 10.5LSAMLEE148 pKa = 4.21EE149 pKa = 4.23ARR151 pKa = 11.84KK152 pKa = 9.56RR153 pKa = 11.84PEE155 pKa = 3.81AEE157 pKa = 3.88VVLDD161 pKa = 3.88YY162 pKa = 11.65QEE164 pKa = 4.05GG165 pKa = 3.55

Molecular weight: 18.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411CW91|A0A411CW91_9CAUD Uncharacterized protein OS=Thermus phage TSP4 OX=1298530 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84VLLLAVLLLLSTFALTPSSKK22 pKa = 10.69SYY24 pKa = 10.41TKK26 pKa = 9.98IPKK29 pKa = 9.73FKK31 pKa = 10.92VRR33 pKa = 11.84VTATAYY39 pKa = 9.05TSLAHH44 pKa = 6.29LTDD47 pKa = 3.46STPFLTASMTRR58 pKa = 11.84TRR60 pKa = 11.84VEE62 pKa = 3.88YY63 pKa = 10.52RR64 pKa = 11.84NGVQWHH70 pKa = 5.02VVAVSRR76 pKa = 11.84DD77 pKa = 3.47LLTVFPYY84 pKa = 10.3GSKK87 pKa = 10.69VFLRR91 pKa = 11.84CPNRR95 pKa = 11.84LIYY98 pKa = 10.66GLVEE102 pKa = 4.11DD103 pKa = 4.49TMHH106 pKa = 5.78IRR108 pKa = 11.84WRR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84VDD114 pKa = 2.37IWFPDD119 pKa = 3.82LSQALRR125 pKa = 11.84WGKK128 pKa = 10.08KK129 pKa = 7.22EE130 pKa = 4.24CILEE134 pKa = 4.11GRR136 pKa = 4.21

Molecular weight: 15.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

26531

67

5029

270.7

30.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.974 ± 0.589

0.441 ± 0.1

5.047 ± 0.236

7.173 ± 0.462

3.826 ± 0.198

7.12 ± 0.218

1.813 ± 0.239

3.788 ± 0.186

4.968 ± 0.313

11.635 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.085

3.238 ± 0.181

5.201 ± 0.375

4.161 ± 0.305

6.479 ± 0.254

5.823 ± 0.195

4.907 ± 0.281

7.712 ± 0.369

1.519 ± 0.209

4.123 ± 0.278
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski