Amino acid dipepetide frequency for Bacillus sp. TS-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.312AlaAla: 4.312 ± 0.075	0.547AlaCys: 0.547 ± 0.024	2.935AlaAsp: 2.935 ± 0.052	4.22AlaGlu: 4.22 ± 0.063	3.066AlaPhe: 3.066 ± 0.057	4.234AlaGly: 4.234 ± 0.076	1.254AlaHis: 1.254 ± 0.033	5.54AlaIle: 5.54 ± 0.081	3.999AlaLys: 3.999 ± 0.063	6.628AlaLeu: 6.628 ± 0.083	1.651AlaMet: 1.651 ± 0.031	2.754AlaAsn: 2.754 ± 0.055	1.844AlaPro: 1.844 ± 0.043	2.198AlaGln: 2.198 ± 0.045	2.238AlaArg: 2.238 ± 0.047	3.894AlaSer: 3.894 ± 0.056	3.34AlaThr: 3.34 ± 0.049	4.282AlaVal: 4.282 ± 0.066	0.562AlaTrp: 0.562 ± 0.027	2.045AlaTyr: 2.045 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.325CysAla: 0.325 ± 0.017	0.083CysCys: 0.083 ± 0.008	0.333CysAsp: 0.333 ± 0.019	0.416CysGlu: 0.416 ± 0.018	0.313CysPhe: 0.313 ± 0.014	0.525CysGly: 0.525 ± 0.025	0.223CysHis: 0.223 ± 0.014	0.462CysIle: 0.462 ± 0.019	0.329CysLys: 0.329 ± 0.017	0.676CysLeu: 0.676 ± 0.026	0.158CysMet: 0.158 ± 0.011	0.263CysAsn: 0.263 ± 0.016	0.267CysPro: 0.267 ± 0.016	0.29CysGln: 0.29 ± 0.015	0.225CysArg: 0.225 ± 0.013	0.465CysSer: 0.465 ± 0.02	0.332CysThr: 0.332 ± 0.016	0.36CysVal: 0.36 ± 0.02	0.054CysTrp: 0.054 ± 0.007	0.262CysTyr: 0.262 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	2.726AspAla: 2.726 ± 0.055	0.343AspCys: 0.343 ± 0.015	2.629AspAsp: 2.629 ± 0.053	4.658AspGlu: 4.658 ± 0.082	2.727AspPhe: 2.727 ± 0.046	3.331AspGly: 3.331 ± 0.056	1.197AspHis: 1.197 ± 0.034	4.209AspIle: 4.209 ± 0.059	2.718AspLys: 2.718 ± 0.054	5.033AspLeu: 5.033 ± 0.064	1.314AspMet: 1.314 ± 0.033	1.725AspAsn: 1.725 ± 0.048	1.814AspPro: 1.814 ± 0.046	2.223AspGln: 2.223 ± 0.048	1.954AspArg: 1.954 ± 0.039	3.005AspSer: 3.005 ± 0.056	2.331AspThr: 2.331 ± 0.043	3.472AspVal: 3.472 ± 0.052	0.704AspTrp: 0.704 ± 0.028	2.484AspTyr: 2.484 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	5.339GluAla: 5.339 ± 0.079	0.357GluCys: 0.357 ± 0.016	4.042GluAsp: 4.042 ± 0.064	8.362GluGlu: 8.362 ± 0.111	2.664GluPhe: 2.664 ± 0.054	4.578GluGly: 4.578 ± 0.073	1.803GluHis: 1.803 ± 0.042	6.044GluIle: 6.044 ± 0.075	6.705GluLys: 6.705 ± 0.097	7.746GluLeu: 7.746 ± 0.1	2.469GluMet: 2.469 ± 0.047	4.335GluAsn: 4.335 ± 0.067	2.215GluPro: 2.215 ± 0.052	4.196GluGln: 4.196 ± 0.065	3.741GluArg: 3.741 ± 0.061	4.201GluSer: 4.201 ± 0.067	3.716GluThr: 3.716 ± 0.063	5.464GluVal: 5.464 ± 0.07	0.956GluTrp: 0.956 ± 0.031	2.448GluTyr: 2.448 ± 0.057	0.0GluXaa: 0.0 ± 0.0
Phe	2.771PheAla: 2.771 ± 0.052	0.32PheCys: 0.32 ± 0.016	2.428PheAsp: 2.428 ± 0.049	3.223PheGlu: 3.223 ± 0.048	2.51PhePhe: 2.51 ± 0.056	3.066PheGly: 3.066 ± 0.063	1.046PheHis: 1.046 ± 0.03	4.445PheIle: 4.445 ± 0.078	2.521PheLys: 2.521 ± 0.052	4.902PheLeu: 4.902 ± 0.077	1.172PheMet: 1.172 ± 0.033	2.093PheAsn: 2.093 ± 0.04	1.596PhePro: 1.596 ± 0.04	1.832PheGln: 1.832 ± 0.041	1.465PheArg: 1.465 ± 0.036	3.643PheSer: 3.643 ± 0.06	2.528PheThr: 2.528 ± 0.046	3.026PheVal: 3.026 ± 0.051	0.518PheTrp: 0.518 ± 0.022	1.876PheTyr: 1.876 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.282GlyAla: 4.282 ± 0.078	0.537GlyCys: 0.537 ± 0.024	3.037GlyAsp: 3.037 ± 0.063	4.755GlyGlu: 4.755 ± 0.068	3.291GlyPhe: 3.291 ± 0.056	4.287GlyGly: 4.287 ± 0.079	1.372GlyHis: 1.372 ± 0.036	5.364GlyIle: 5.364 ± 0.07	4.156GlyLys: 4.156 ± 0.063	6.168GlyLeu: 6.168 ± 0.088	1.829GlyMet: 1.829 ± 0.046	2.637GlyAsn: 2.637 ± 0.048	1.731GlyPro: 1.731 ± 0.05	2.318GlyGln: 2.318 ± 0.044	2.318GlyArg: 2.318 ± 0.054	3.736GlySer: 3.736 ± 0.06	3.506GlyThr: 3.506 ± 0.06	4.53GlyVal: 4.53 ± 0.071	0.757GlyTrp: 0.757 ± 0.027	2.732GlyTyr: 2.732 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.172HisAla: 1.172 ± 0.031	0.19HisCys: 0.19 ± 0.013	1.088HisAsp: 1.088 ± 0.031	1.508HisGlu: 1.508 ± 0.034	1.309HisPhe: 1.309 ± 0.034	1.247HisGly: 1.247 ± 0.037	0.781HisHis: 0.781 ± 0.028	1.675HisIle: 1.675 ± 0.036	1.062HisLys: 1.062 ± 0.03	2.471HisLeu: 2.471 ± 0.053	0.475HisMet: 0.475 ± 0.02	0.894HisAsn: 0.894 ± 0.03	1.007HisPro: 1.007 ± 0.033	1.087HisGln: 1.087 ± 0.032	0.855HisArg: 0.855 ± 0.027	1.629HisSer: 1.629 ± 0.037	1.085HisThr: 1.085 ± 0.029	1.34HisVal: 1.34 ± 0.035	0.255HisTrp: 0.255 ± 0.015	1.131HisTyr: 1.131 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.457IleAla: 5.457 ± 0.081	0.596IleCys: 0.596 ± 0.024	4.619IleAsp: 4.619 ± 0.074	6.834IleGlu: 6.834 ± 0.085	3.583IlePhe: 3.583 ± 0.068	5.754IleGly: 5.754 ± 0.079	1.927IleHis: 1.927 ± 0.04	6.527IleIle: 6.527 ± 0.089	4.999IleLys: 4.999 ± 0.074	7.548IleLeu: 7.548 ± 0.094	1.907IleMet: 1.907 ± 0.042	3.58IleAsn: 3.58 ± 0.058	3.31IlePro: 3.31 ± 0.064	3.223IleGln: 3.223 ± 0.058	3.108IleArg: 3.108 ± 0.057	5.605IleSer: 5.605 ± 0.087	4.394IleThr: 4.394 ± 0.063	5.288IleVal: 5.288 ± 0.068	0.782IleTrp: 0.782 ± 0.026	2.676IleTyr: 2.676 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.044LysAla: 4.044 ± 0.067	0.281LysCys: 0.281 ± 0.015	3.316LysAsp: 3.316 ± 0.054	7.183LysGlu: 7.183 ± 0.097	1.675LysPhe: 1.675 ± 0.042	4.192LysGly: 4.192 ± 0.068	1.423LysHis: 1.423 ± 0.039	4.652LysIle: 4.652 ± 0.066	6.279LysLys: 6.279 ± 0.095	5.607LysLeu: 5.607 ± 0.08	2.101LysMet: 2.101 ± 0.04	3.546LysAsn: 3.546 ± 0.056	2.122LysPro: 2.122 ± 0.043	3.265LysGln: 3.265 ± 0.061	3.276LysArg: 3.276 ± 0.054	3.664LysSer: 3.664 ± 0.051	3.185LysThr: 3.185 ± 0.049	4.523LysVal: 4.523 ± 0.065	0.885LysTrp: 0.885 ± 0.031	2.015LysTyr: 2.015 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	6.636LeuAla: 6.636 ± 0.084	0.62LeuCys: 0.62 ± 0.023	4.99LeuAsp: 4.99 ± 0.066	7.705LeuGlu: 7.705 ± 0.102	5.204LeuPhe: 5.204 ± 0.08	5.856LeuGly: 5.856 ± 0.078	1.95LeuHis: 1.95 ± 0.042	7.969LeuIle: 7.969 ± 0.098	6.954LeuLys: 6.954 ± 0.095	10.62LeuLeu: 10.62 ± 0.11	2.577LeuMet: 2.577 ± 0.047	4.854LeuAsn: 4.854 ± 0.063	3.79LeuPro: 3.79 ± 0.066	3.832LeuGln: 3.832 ± 0.054	3.374LeuArg: 3.374 ± 0.052	7.308LeuSer: 7.308 ± 0.084	5.701LeuThr: 5.701 ± 0.074	5.988LeuVal: 5.988 ± 0.076	0.836LeuTrp: 0.836 ± 0.03	3.328LeuTyr: 3.328 ± 0.058	0.0LeuXaa: 0.0 ± 0.0
Met	1.781MetAla: 1.781 ± 0.042	0.116MetCys: 0.116 ± 0.01	1.397MetAsp: 1.397 ± 0.033	1.862MetGlu: 1.862 ± 0.04	1.016MetPhe: 1.016 ± 0.029	1.504MetGly: 1.504 ± 0.042	0.37MetHis: 0.37 ± 0.017	2.36MetIle: 2.36 ± 0.047	2.441MetLys: 2.441 ± 0.042	2.481MetLeu: 2.481 ± 0.051	0.846MetMet: 0.846 ± 0.03	1.83MetAsn: 1.83 ± 0.04	0.865MetPro: 0.865 ± 0.028	0.789MetGln: 0.789 ± 0.027	1.033MetArg: 1.033 ± 0.03	1.827MetSer: 1.827 ± 0.039	1.672MetThr: 1.672 ± 0.04	1.819MetVal: 1.819 ± 0.035	0.197MetTrp: 0.197 ± 0.014	0.701MetTyr: 0.701 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.363AsnAla: 2.363 ± 0.048	0.266AsnCys: 0.266 ± 0.017	2.552AsnAsp: 2.552 ± 0.047	4.495AsnGlu: 4.495 ± 0.071	1.669AsnPhe: 1.669 ± 0.035	3.396AsnGly: 3.396 ± 0.068	1.436AsnHis: 1.436 ± 0.035	3.447AsnIle: 3.447 ± 0.058	3.056AsnLys: 3.056 ± 0.056	3.962AsnLeu: 3.962 ± 0.067	1.197AsnMet: 1.197 ± 0.03	2.141AsnAsn: 2.141 ± 0.058	2.215AsnPro: 2.215 ± 0.048	2.913AsnGln: 2.913 ± 0.058	2.104AsnArg: 2.104 ± 0.042	2.752AsnSer: 2.752 ± 0.056	2.209AsnThr: 2.209 ± 0.051	2.92AsnVal: 2.92 ± 0.054	0.654AsnTrp: 0.654 ± 0.024	1.701AsnTyr: 1.701 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	1.904ProAla: 1.904 ± 0.043	0.178ProCys: 0.178 ± 0.013	1.782ProAsp: 1.782 ± 0.036	2.795ProGlu: 2.795 ± 0.063	2.045ProPhe: 2.045 ± 0.039	1.972ProGly: 1.972 ± 0.056	0.807ProHis: 0.807 ± 0.029	2.991ProIle: 2.991 ± 0.048	2.002ProLys: 2.002 ± 0.041	3.621ProLeu: 3.621 ± 0.065	0.754ProMet: 0.754 ± 0.027	1.815ProAsn: 1.815 ± 0.038	0.955ProPro: 0.955 ± 0.027	1.129ProGln: 1.129 ± 0.031	0.949ProArg: 0.949 ± 0.026	2.349ProSer: 2.349 ± 0.042	2.009ProThr: 2.009 ± 0.044	2.367ProVal: 2.367 ± 0.055	0.369ProTrp: 0.369 ± 0.02	1.399ProTyr: 1.399 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	2.579GlnAla: 2.579 ± 0.05	0.189GlnCys: 0.189 ± 0.012	1.786GlnAsp: 1.786 ± 0.036	3.268GlnGlu: 3.268 ± 0.058	1.928GlnPhe: 1.928 ± 0.037	2.112GlnGly: 2.112 ± 0.044	0.855GlnHis: 0.855 ± 0.028	3.259GlnIle: 3.259 ± 0.049	3.015GlnLys: 3.015 ± 0.053	4.919GlnLeu: 4.919 ± 0.066	1.226GlnMet: 1.226 ± 0.036	2.011GlnAsn: 2.011 ± 0.043	1.221GlnPro: 1.221 ± 0.036	1.93GlnGln: 1.93 ± 0.048	1.526GlnArg: 1.526 ± 0.036	2.795GlnSer: 2.795 ± 0.054	2.193GlnThr: 2.193 ± 0.051	2.613GlnVal: 2.613 ± 0.05	0.495GlnTrp: 0.495 ± 0.023	1.569GlnTyr: 1.569 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.26ArgAla: 2.26 ± 0.051	0.212ArgCys: 0.212 ± 0.014	1.937ArgAsp: 1.937 ± 0.044	3.038ArgGlu: 3.038 ± 0.056	1.894ArgPhe: 1.894 ± 0.036	2.172ArgGly: 2.172 ± 0.047	0.748ArgHis: 0.748 ± 0.028	3.008ArgIle: 3.008 ± 0.055	2.858ArgLys: 2.858 ± 0.055	3.995ArgLeu: 3.995 ± 0.058	1.189ArgMet: 1.189 ± 0.031	1.965ArgAsn: 1.965 ± 0.049	1.132ArgPro: 1.132 ± 0.034	1.44ArgGln: 1.44 ± 0.034	1.616ArgArg: 1.616 ± 0.041	2.209ArgSer: 2.209 ± 0.045	1.953ArgThr: 1.953 ± 0.047	2.462ArgVal: 2.462 ± 0.044	0.433ArgTrp: 0.433 ± 0.017	1.597ArgTyr: 1.597 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.43SerAla: 3.43 ± 0.052	0.363SerCys: 0.363 ± 0.018	3.029SerAsp: 3.029 ± 0.051	4.658SerGlu: 4.658 ± 0.061	3.781SerPhe: 3.781 ± 0.064	4.152SerGly: 4.152 ± 0.062	1.389SerHis: 1.389 ± 0.037	5.846SerIle: 5.846 ± 0.077	4.071SerLys: 4.071 ± 0.063	7.073SerLeu: 7.073 ± 0.088	1.787SerMet: 1.787 ± 0.04	3.17SerAsn: 3.17 ± 0.061	2.079SerPro: 2.079 ± 0.041	2.435SerGln: 2.435 ± 0.048	2.148SerArg: 2.148 ± 0.039	4.535SerSer: 4.535 ± 0.079	3.355SerThr: 3.355 ± 0.055	4.037SerVal: 4.037 ± 0.052	0.716SerTrp: 0.716 ± 0.027	2.55SerTyr: 2.55 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	3.248ThrAla: 3.248 ± 0.046	0.314ThrCys: 0.314 ± 0.017	2.647ThrAsp: 2.647 ± 0.048	3.667ThrGlu: 3.667 ± 0.061	2.764ThrPhe: 2.764 ± 0.049	3.637ThrGly: 3.637 ± 0.069	1.04ThrHis: 1.04 ± 0.032	4.921ThrIle: 4.921 ± 0.059	3.222ThrLys: 3.222 ± 0.061	5.29ThrLeu: 5.29 ± 0.075	1.25ThrMet: 1.25 ± 0.034	2.659ThrAsn: 2.659 ± 0.055	2.112ThrPro: 2.112 ± 0.046	1.517ThrGln: 1.517 ± 0.032	1.651ThrArg: 1.651 ± 0.037	3.326ThrSer: 3.326 ± 0.059	2.864ThrThr: 2.864 ± 0.056	3.913ThrVal: 3.913 ± 0.068	0.54ThrTrp: 0.54 ± 0.024	1.981ThrTyr: 1.981 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	4.43ValAla: 4.43 ± 0.058	0.494ValCys: 0.494 ± 0.022	3.531ValAsp: 3.531 ± 0.051	5.132ValGlu: 5.132 ± 0.076	2.989ValPhe: 2.989 ± 0.058	4.355ValGly: 4.355 ± 0.067	1.368ValHis: 1.368 ± 0.035	5.55ValIle: 5.55 ± 0.076	4.097ValLys: 4.097 ± 0.065	6.329ValLeu: 6.329 ± 0.079	1.751ValMet: 1.751 ± 0.041	3.03ValAsn: 3.03 ± 0.049	2.235ValPro: 2.235 ± 0.05	2.38ValGln: 2.38 ± 0.042	2.457ValArg: 2.457 ± 0.045	4.427ValSer: 4.427 ± 0.054	3.962ValThr: 3.962 ± 0.061	4.384ValVal: 4.384 ± 0.067	0.6ValTrp: 0.6 ± 0.02	2.152ValTyr: 2.152 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.574TrpAla: 0.574 ± 0.025	0.083TrpCys: 0.083 ± 0.009	0.527TrpAsp: 0.527 ± 0.023	0.738TrpGlu: 0.738 ± 0.023	0.563TrpPhe: 0.563 ± 0.025	0.651TrpGly: 0.651 ± 0.022	0.227TrpHis: 0.227 ± 0.014	0.851TrpIle: 0.851 ± 0.025	0.797TrpLys: 0.797 ± 0.024	1.222TrpLeu: 1.222 ± 0.036	0.361TrpMet: 0.361 ± 0.018	0.633TrpAsn: 0.633 ± 0.024	0.287TrpPro: 0.287 ± 0.017	0.45TrpGln: 0.45 ± 0.021	0.441TrpArg: 0.441 ± 0.021	0.773TrpSer: 0.773 ± 0.027	0.524TrpThr: 0.524 ± 0.021	0.635TrpVal: 0.635 ± 0.027	0.146TrpTrp: 0.146 ± 0.011	0.39TrpTyr: 0.39 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.909TyrAla: 1.909 ± 0.042	0.273TyrCys: 0.273 ± 0.015	1.995TyrAsp: 1.995 ± 0.05	2.864TyrGlu: 2.864 ± 0.049	1.989TyrPhe: 1.989 ± 0.038	2.322TyrGly: 2.322 ± 0.042	1.044TyrHis: 1.044 ± 0.035	2.597TyrIle: 2.597 ± 0.043	1.952TyrLys: 1.952 ± 0.04	3.801TyrLeu: 3.801 ± 0.056	0.895TyrMet: 0.895 ± 0.025	1.515TyrAsn: 1.515 ± 0.039	1.397TyrPro: 1.397 ± 0.034	2.04TyrGln: 2.04 ± 0.047	1.638TyrArg: 1.638 ± 0.041	2.459TyrSer: 2.459 ± 0.049	1.703TyrThr: 1.703 ± 0.04	2.23TyrVal: 2.23 ± 0.041	0.415TyrTrp: 0.415 ± 0.02	1.609TyrTyr: 1.609 ± 0.048	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4084 proteins (1205149 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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