Streptomyces sp. Ag109_G2-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N0FNK4|A0A2N0FNK4_9ACTN XRE family transcriptional regulator OS=Streptomyces sp. Ag109_G2-1 OX=1938849 GN=BX280_6914 PE=4 SV=1
MM1 pKa = 6.4QQEE4 pKa = 4.51VPAGGSGGAGEE15 pKa = 4.59PLEE18 pKa = 4.2VWIDD22 pKa = 3.44QDD24 pKa = 3.99LCTGDD29 pKa = 4.65GICVQYY35 pKa = 10.96APEE38 pKa = 4.18VFEE41 pKa = 5.9LDD43 pKa = 3.05IDD45 pKa = 3.63GLAYY49 pKa = 10.45VKK51 pKa = 10.83SPEE54 pKa = 4.4DD55 pKa = 3.55EE56 pKa = 4.47LLVEE60 pKa = 5.2PGATTPVPLPLLRR73 pKa = 11.84DD74 pKa = 3.74VVDD77 pKa = 4.03SAKK80 pKa = 10.21EE81 pKa = 3.92CPGDD85 pKa = 4.18CIHH88 pKa = 6.36VRR90 pKa = 11.84RR91 pKa = 11.84VSDD94 pKa = 3.3RR95 pKa = 11.84VEE97 pKa = 4.16VYY99 pKa = 10.89GPDD102 pKa = 3.45AEE104 pKa = 4.31

Molecular weight: 11.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N0H2K0|A0A2N0H2K0_9ACTN Secreted protein OS=Streptomyces sp. Ag109_G2-1 OX=1938849 GN=BX280_5548 PE=4 SV=1
MM1 pKa = 6.82VASAATTAVVAPPVAASAVTTAADD25 pKa = 3.34VRR27 pKa = 11.84PAVASVAVTTAAATVRR43 pKa = 11.84RR44 pKa = 11.84TPAVMTSAVVSVAVAMTVVGTVRR67 pKa = 11.84RR68 pKa = 11.84TRR70 pKa = 11.84VVMTAVATVPLTRR83 pKa = 11.84VVMTSVVVSVAVAMTVAATARR104 pKa = 11.84PSVVTTGAATVRR116 pKa = 11.84SRR118 pKa = 11.84RR119 pKa = 11.84VVTTTVVVSVAVVTTVVGTVRR140 pKa = 11.84RR141 pKa = 11.84TRR143 pKa = 11.84VVMTVGTVPLTRR155 pKa = 11.84VVMTSVVVSVAVVTTVVATVRR176 pKa = 11.84RR177 pKa = 11.84TRR179 pKa = 11.84VVMTAVATVRR189 pKa = 11.84PTPVVTTSVVVSVAVVTTAVGTVRR213 pKa = 11.84PTPVVMTSAVVSVATVRR230 pKa = 11.84PSVVTTGVASVPPSVVMTVGTVLPTRR256 pKa = 11.84VVTMSVAVSVAVVTTVAATVRR277 pKa = 11.84RR278 pKa = 11.84TRR280 pKa = 11.84VVTTAVATVPPTPVVMTSVVGSVVATVPPSAVTSGAATVRR320 pKa = 11.84SRR322 pKa = 11.84RR323 pKa = 11.84VGTTTVAGVLPTRR336 pKa = 11.84VVMTSAAVSVAAVTTAVATVRR357 pKa = 11.84PTGVVTTVVGTVPRR371 pKa = 11.84TAAVTTGVTVRR382 pKa = 11.84PTRR385 pKa = 11.84VVTTGAASVVATVVAAMTAVVSVAVARR412 pKa = 11.84SGAAVSSVPTATGTRR427 pKa = 11.84SSGFRR432 pKa = 11.84STTTSPATRR441 pKa = 11.84STPMCARR448 pKa = 11.84SSS450 pKa = 3.42

Molecular weight: 44.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7108

0

7108

2357120

27

9406

331.6

35.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.476 ± 0.059

0.818 ± 0.008

5.609 ± 0.022

5.695 ± 0.027

2.658 ± 0.017

9.821 ± 0.036

2.274 ± 0.014

2.911 ± 0.025

2.066 ± 0.027

10.419 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.682 ± 0.012

1.667 ± 0.017

6.533 ± 0.033

2.715 ± 0.017

8.066 ± 0.036

4.887 ± 0.021

5.918 ± 0.029

8.225 ± 0.033

1.528 ± 0.013

2.032 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski