Apis mellifera associated microvirus 61

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5U3|A0A3Q8U5U3_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 61 OX=2494793 PE=4 SV=1
MM1 pKa = 7.18YY2 pKa = 9.62GQSNMHH8 pKa = 5.41VQGPQINQFNNIAPEE23 pKa = 3.94PVPRR27 pKa = 11.84SMFKK31 pKa = 10.32RR32 pKa = 11.84DD33 pKa = 3.3FTRR36 pKa = 11.84RR37 pKa = 11.84STFDD41 pKa = 3.48PDD43 pKa = 3.49YY44 pKa = 10.65LVPIFLDD51 pKa = 4.35EE52 pKa = 4.36ILPGDD57 pKa = 4.0EE58 pKa = 5.06VNLKK62 pKa = 9.21MNAFVRR68 pKa = 11.84MSTPIKK74 pKa = 10.17PLMDD78 pKa = 3.79NLYY81 pKa = 11.01LEE83 pKa = 4.77TFWFFVPNRR92 pKa = 11.84LVWDD96 pKa = 3.44NWEE99 pKa = 4.25KK100 pKa = 10.89FQGQQTNPGDD110 pKa = 4.02SVDD113 pKa = 3.67YY114 pKa = 8.84TIPVLDD120 pKa = 4.26DD121 pKa = 3.37TVAPLSTTGFPVEE134 pKa = 4.48SIYY137 pKa = 10.8DD138 pKa = 3.81YY139 pKa = 11.19FGLPVLVTGIEE150 pKa = 4.64GISCLFNRR158 pKa = 11.84AYY160 pKa = 10.79DD161 pKa = 4.46LIWNEE166 pKa = 3.65WFRR169 pKa = 11.84DD170 pKa = 3.58EE171 pKa = 5.2NLQNSLTVPTGDD183 pKa = 5.25GPDD186 pKa = 3.84DD187 pKa = 3.91PANYY191 pKa = 9.14TLRR194 pKa = 11.84KK195 pKa = 8.2RR196 pKa = 11.84NKK198 pKa = 8.71RR199 pKa = 11.84KK200 pKa = 10.43DD201 pKa = 3.62YY202 pKa = 7.86FTSMLPWPQKK212 pKa = 10.17GDD214 pKa = 3.16AVMIPLAGSAPVISNGQVPKK234 pKa = 10.78LQATSSGNFGNLANSNTIGLTLSSPSGSIGNEE266 pKa = 3.48DD267 pKa = 3.41LRR269 pKa = 11.84FYY271 pKa = 7.67TTNTGLEE278 pKa = 3.97ADD280 pKa = 4.43LDD282 pKa = 4.23AVSALSINVLRR293 pKa = 11.84QSALYY298 pKa = 9.65QQILEE303 pKa = 4.13LDD305 pKa = 3.37ARR307 pKa = 11.84GGTRR311 pKa = 11.84YY312 pKa = 9.89VEE314 pKa = 4.27SVYY317 pKa = 11.12SRR319 pKa = 11.84FGVILPDD326 pKa = 3.83FRR328 pKa = 11.84VQRR331 pKa = 11.84PEE333 pKa = 4.11LLGQGSTRR341 pKa = 11.84INIYY345 pKa = 10.26AVPQTAPSTDD355 pKa = 4.16DD356 pKa = 3.61SPQGNLAAFGTAQISGNHH374 pKa = 5.09GCRR377 pKa = 11.84YY378 pKa = 9.82AATEE382 pKa = 3.79HH383 pKa = 6.22GMLIGLANVRR393 pKa = 11.84ADD395 pKa = 3.35LTYY398 pKa = 10.67QQGVPKK404 pKa = 10.37KK405 pKa = 9.02FLRR408 pKa = 11.84STRR411 pKa = 11.84LDD413 pKa = 3.58FYY415 pKa = 11.56EE416 pKa = 4.36PLQNGLGEE424 pKa = 3.88QSVLNKK430 pKa = 9.93EE431 pKa = 4.98LYY433 pKa = 10.3AQGTSADD440 pKa = 3.96EE441 pKa = 4.41DD442 pKa = 3.85VAGYY446 pKa = 7.16QEE448 pKa = 5.0RR449 pKa = 11.84FAEE452 pKa = 4.15YY453 pKa = 9.56RR454 pKa = 11.84WFNSEE459 pKa = 3.61VTGKK463 pKa = 9.66FRR465 pKa = 11.84SVAPTTLDD473 pKa = 3.04VWHH476 pKa = 7.24LSQEE480 pKa = 4.39FGSLPLLNSSFITEE494 pKa = 4.68DD495 pKa = 3.27VPMDD499 pKa = 3.46RR500 pKa = 11.84VLATGSTEE508 pKa = 3.88PAFLYY513 pKa = 9.92DD514 pKa = 2.93AHH516 pKa = 7.44YY517 pKa = 10.54SYY519 pKa = 9.76THH521 pKa = 5.65VRR523 pKa = 11.84PMPIRR528 pKa = 11.84SNPGIRR534 pKa = 11.84RR535 pKa = 11.84LL536 pKa = 3.55

Molecular weight: 59.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UU75|A0A3S8UU75_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 61 OX=2494793 PE=4 SV=1
MM1 pKa = 7.23CTRR4 pKa = 11.84EE5 pKa = 3.44ITAYY9 pKa = 10.1QNPSGGRR16 pKa = 11.84PIFGWEE22 pKa = 4.09GKK24 pKa = 10.47KK25 pKa = 10.47NGLPEE30 pKa = 4.65LKK32 pKa = 10.01LPCGKK37 pKa = 10.38CPEE40 pKa = 4.36CCKK43 pKa = 10.63DD44 pKa = 4.42YY45 pKa = 9.19YY46 pKa = 10.63TSWATRR52 pKa = 11.84GSRR55 pKa = 11.84EE56 pKa = 3.78LALWDD61 pKa = 3.33STVFITLTYY70 pKa = 10.22SDD72 pKa = 4.06EE73 pKa = 4.41HH74 pKa = 7.04LPPDD78 pKa = 3.38NSLNKK83 pKa = 9.86KK84 pKa = 9.86DD85 pKa = 3.52VQDD88 pKa = 4.49FIKK91 pKa = 10.4RR92 pKa = 11.84VKK94 pKa = 10.46KK95 pKa = 10.22RR96 pKa = 11.84FKK98 pKa = 9.49STKK101 pKa = 9.07EE102 pKa = 3.54NPIRR106 pKa = 11.84QIYY109 pKa = 8.75CGEE112 pKa = 4.02YY113 pKa = 10.16GEE115 pKa = 4.43KK116 pKa = 9.45TKK118 pKa = 10.66RR119 pKa = 11.84PHH121 pKa = 4.64YY122 pKa = 10.11HH123 pKa = 7.34AILFNCDD130 pKa = 3.08FADD133 pKa = 3.94KK134 pKa = 9.97KK135 pKa = 8.98PHH137 pKa = 5.53YY138 pKa = 8.51MSPQGHH144 pKa = 5.03QVYY147 pKa = 8.62TSQTLNNLWKK157 pKa = 10.88NGFAEE162 pKa = 4.81FGYY165 pKa = 10.3AQPGSIAYY173 pKa = 9.3LFKK176 pKa = 11.16YY177 pKa = 9.53VLKK180 pKa = 10.64KK181 pKa = 10.33KK182 pKa = 9.66SRR184 pKa = 11.84KK185 pKa = 8.82EE186 pKa = 3.68KK187 pKa = 9.35EE188 pKa = 3.65QPLIIEE194 pKa = 4.51RR195 pKa = 11.84DD196 pKa = 3.51GVTYY200 pKa = 10.25EE201 pKa = 4.05VAHH204 pKa = 6.02EE205 pKa = 4.42FIEE208 pKa = 4.36ASRR211 pKa = 11.84NPGIGAHH218 pKa = 5.6MRR220 pKa = 11.84NSDD223 pKa = 4.31SIKK226 pKa = 10.48KK227 pKa = 10.51GYY229 pKa = 8.67LTVNGVKK236 pKa = 10.27KK237 pKa = 10.52KK238 pKa = 10.08LSKK241 pKa = 11.08YY242 pKa = 8.01YY243 pKa = 10.8LEE245 pKa = 4.31WLRR248 pKa = 11.84KK249 pKa = 9.28NDD251 pKa = 4.07PDD253 pKa = 3.95TFDD256 pKa = 4.85NISNMKK262 pKa = 9.84FDD264 pKa = 5.24FMCKK268 pKa = 9.74LPKK271 pKa = 9.9EE272 pKa = 4.03SRR274 pKa = 11.84LRR276 pKa = 11.84KK277 pKa = 7.57EE278 pKa = 4.43QKK280 pKa = 10.03EE281 pKa = 4.07KK282 pKa = 10.79AQKK285 pKa = 10.87KK286 pKa = 8.08LTDD289 pKa = 3.65TKK291 pKa = 11.23KK292 pKa = 10.9KK293 pKa = 10.52LL294 pKa = 3.39

Molecular weight: 34.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1253

77

536

250.6

28.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.704 ± 1.39

0.878 ± 0.526

6.464 ± 0.821

5.347 ± 0.506

4.469 ± 0.618

7.103 ± 0.398

1.596 ± 0.299

5.108 ± 0.614

8.14 ± 2.769

7.901 ± 0.856

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.995 ± 0.127

5.427 ± 0.453

6.145 ± 0.762

5.347 ± 0.959

4.549 ± 0.764

7.023 ± 0.513

6.145 ± 0.359

4.868 ± 0.861

1.117 ± 0.303

3.671 ± 0.71
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski