Amino acid dipepetide frequency for Acinetobacter phage phiAB6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.831AlaAla: 7.831 ± 1.033
0.495AlaCys: 0.495 ± 0.245
5.441AlaAsp: 5.441 ± 0.904
5.193AlaGlu: 5.193 ± 0.568
2.473AlaPhe: 2.473 ± 0.386
6.924AlaGly: 6.924 ± 0.528
1.649AlaHis: 1.649 ± 0.391
5.028AlaIle: 5.028 ± 0.79
5.193AlaLys: 5.193 ± 0.546
8.985AlaLeu: 8.985 ± 0.761
3.05AlaMet: 3.05 ± 0.506
3.957AlaAsn: 3.957 ± 0.498
2.391AlaPro: 2.391 ± 0.559
4.616AlaGln: 4.616 ± 0.679
2.308AlaArg: 2.308 ± 0.514
3.874AlaSer: 3.874 ± 0.613
4.781AlaThr: 4.781 ± 0.562
5.111AlaVal: 5.111 ± 0.714
0.824AlaTrp: 0.824 ± 0.281
3.215AlaTyr: 3.215 ± 0.428
0.0AlaXaa: 0.0 ± 0.0
Cys
0.659CysAla: 0.659 ± 0.246
0.165CysCys: 0.165 ± 0.115
1.237CysAsp: 1.237 ± 0.361
0.412CysGlu: 0.412 ± 0.228
0.495CysPhe: 0.495 ± 0.189
1.401CysGly: 1.401 ± 0.388
0.33CysHis: 0.33 ± 0.148
1.072CysIle: 1.072 ± 0.385
0.082CysLys: 0.082 ± 0.097
1.072CysLeu: 1.072 ± 0.38
0.0CysMet: 0.0 ± 0.0
0.247CysAsn: 0.247 ± 0.159
0.33CysPro: 0.33 ± 0.16
0.165CysGln: 0.165 ± 0.107
0.247CysArg: 0.247 ± 0.158
0.495CysSer: 0.495 ± 0.203
0.495CysThr: 0.495 ± 0.174
0.989CysVal: 0.989 ± 0.38
0.082CysTrp: 0.082 ± 0.079
0.659CysTyr: 0.659 ± 0.235
0.0CysXaa: 0.0 ± 0.0
Asp
5.358AspAla: 5.358 ± 0.582
1.072AspCys: 1.072 ± 0.346
3.05AspAsp: 3.05 ± 0.576
4.122AspGlu: 4.122 ± 0.557
2.391AspPhe: 2.391 ± 0.34
3.792AspGly: 3.792 ± 0.575
1.484AspHis: 1.484 ± 0.367
4.781AspIle: 4.781 ± 0.619
4.451AspLys: 4.451 ± 0.562
4.781AspLeu: 4.781 ± 0.665
1.649AspMet: 1.649 ± 0.365
3.297AspAsn: 3.297 ± 0.431
1.814AspPro: 1.814 ± 0.357
1.978AspGln: 1.978 ± 0.388
3.132AspArg: 3.132 ± 0.606
3.71AspSer: 3.71 ± 0.5
3.545AspThr: 3.545 ± 0.582
3.957AspVal: 3.957 ± 0.617
1.814AspTrp: 1.814 ± 0.392
2.473AspTyr: 2.473 ± 0.618
0.0AspXaa: 0.0 ± 0.0
Glu
5.935GluAla: 5.935 ± 0.614
0.33GluCys: 0.33 ± 0.155
4.781GluAsp: 4.781 ± 0.54
3.545GluGlu: 3.545 ± 0.608
1.896GluPhe: 1.896 ± 0.455
3.792GluGly: 3.792 ± 0.562
1.566GluHis: 1.566 ± 0.301
3.297GluIle: 3.297 ± 0.727
2.143GluLys: 2.143 ± 0.547
6.43GluLeu: 6.43 ± 0.871
1.814GluMet: 1.814 ± 0.321
2.555GluAsn: 2.555 ± 0.476
2.391GluPro: 2.391 ± 0.408
3.297GluGln: 3.297 ± 0.659
2.638GluArg: 2.638 ± 0.492
2.391GluSer: 2.391 ± 0.424
2.226GluThr: 2.226 ± 0.418
4.122GluVal: 4.122 ± 0.525
1.401GluTrp: 1.401 ± 0.326
3.545GluTyr: 3.545 ± 0.53
0.0GluXaa: 0.0 ± 0.0
Phe
2.391PheAla: 2.391 ± 0.568
0.495PheCys: 0.495 ± 0.225
2.391PheAsp: 2.391 ± 0.511
2.638PheGlu: 2.638 ± 0.375
0.989PhePhe: 0.989 ± 0.304
2.72PheGly: 2.72 ± 0.567
0.577PheHis: 0.577 ± 0.203
1.814PheIle: 1.814 ± 0.455
2.473PheLys: 2.473 ± 0.391
2.803PheLeu: 2.803 ± 0.509
0.742PheMet: 0.742 ± 0.197
2.308PheAsn: 2.308 ± 0.594
1.319PhePro: 1.319 ± 0.302
1.649PheGln: 1.649 ± 0.342
0.989PheArg: 0.989 ± 0.288
2.72PheSer: 2.72 ± 0.352
2.308PheThr: 2.308 ± 0.423
1.731PheVal: 1.731 ± 0.429
0.412PheTrp: 0.412 ± 0.201
0.824PheTyr: 0.824 ± 0.325
0.0PheXaa: 0.0 ± 0.0
Gly
5.688GlyAla: 5.688 ± 0.942
0.907GlyCys: 0.907 ± 0.357
3.792GlyAsp: 3.792 ± 0.556
3.132GlyGlu: 3.132 ± 0.619
2.226GlyPhe: 2.226 ± 0.457
5.276GlyGly: 5.276 ± 1.05
0.989GlyHis: 0.989 ± 0.291
4.864GlyIle: 4.864 ± 0.506
6.842GlyLys: 6.842 ± 0.674
5.358GlyLeu: 5.358 ± 0.783
2.885GlyMet: 2.885 ± 0.706
3.792GlyAsn: 3.792 ± 0.727
0.824GlyPro: 0.824 ± 0.304
2.968GlyGln: 2.968 ± 0.585
3.627GlyArg: 3.627 ± 0.4
5.605GlySer: 5.605 ± 0.904
5.853GlyThr: 5.853 ± 0.944
5.358GlyVal: 5.358 ± 0.765
1.401GlyTrp: 1.401 ± 0.349
2.638GlyTyr: 2.638 ± 0.451
0.0GlyXaa: 0.0 ± 0.0
His
1.566HisAla: 1.566 ± 0.39
0.412HisCys: 0.412 ± 0.184
1.566HisAsp: 1.566 ± 0.426
0.824HisGlu: 0.824 ± 0.286
1.154HisPhe: 1.154 ± 0.333
2.143HisGly: 2.143 ± 0.467
0.247HisHis: 0.247 ± 0.126
1.649HisIle: 1.649 ± 0.306
1.484HisLys: 1.484 ± 0.53
1.566HisLeu: 1.566 ± 0.312
0.247HisMet: 0.247 ± 0.147
1.319HisAsn: 1.319 ± 0.348
0.989HisPro: 0.989 ± 0.225
1.072HisGln: 1.072 ± 0.301
0.659HisArg: 0.659 ± 0.203
0.907HisSer: 0.907 ± 0.224
0.247HisThr: 0.247 ± 0.204
1.649HisVal: 1.649 ± 0.269
0.577HisTrp: 0.577 ± 0.201
0.824HisTyr: 0.824 ± 0.289
0.0HisXaa: 0.0 ± 0.0
Ile
4.781IleAla: 4.781 ± 0.6
0.33IleCys: 0.33 ± 0.157
5.193IleAsp: 5.193 ± 0.676
3.71IleGlu: 3.71 ± 0.525
1.401IlePhe: 1.401 ± 0.413
3.132IleGly: 3.132 ± 0.6
1.401IleHis: 1.401 ± 0.352
3.38IleIle: 3.38 ± 0.565
4.534IleLys: 4.534 ± 0.662
3.132IleLeu: 3.132 ± 0.521
1.484IleMet: 1.484 ± 0.362
3.297IleAsn: 3.297 ± 0.366
2.226IlePro: 2.226 ± 0.496
2.72IleGln: 2.72 ± 0.409
3.132IleArg: 3.132 ± 0.614
2.885IleSer: 2.885 ± 0.467
3.297IleThr: 3.297 ± 0.539
3.215IleVal: 3.215 ± 0.638
0.577IleTrp: 0.577 ± 0.215
2.473IleTyr: 2.473 ± 0.362
0.0IleXaa: 0.0 ± 0.0
Lys
5.688LysAla: 5.688 ± 0.824
0.495LysCys: 0.495 ± 0.237
4.039LysAsp: 4.039 ± 0.584
5.523LysGlu: 5.523 ± 0.701
2.143LysPhe: 2.143 ± 0.356
5.853LysGly: 5.853 ± 0.577
1.896LysHis: 1.896 ± 0.447
2.803LysIle: 2.803 ± 0.474
2.803LysLys: 2.803 ± 0.581
5.441LysLeu: 5.441 ± 0.39
1.731LysMet: 1.731 ± 0.306
2.473LysAsn: 2.473 ± 0.335
2.72LysPro: 2.72 ± 0.628
2.803LysGln: 2.803 ± 0.517
3.792LysArg: 3.792 ± 0.69
3.957LysSer: 3.957 ± 0.602
4.287LysThr: 4.287 ± 0.591
4.781LysVal: 4.781 ± 0.728
0.824LysTrp: 0.824 ± 0.24
2.391LysTyr: 2.391 ± 0.398
0.0LysXaa: 0.0 ± 0.0
Leu
6.76LeuAla: 6.76 ± 0.747
0.824LeuCys: 0.824 ± 0.255
5.935LeuAsp: 5.935 ± 0.483
5.276LeuGlu: 5.276 ± 0.865
2.226LeuPhe: 2.226 ± 0.461
4.946LeuGly: 4.946 ± 0.684
1.649LeuHis: 1.649 ± 0.437
4.122LeuIle: 4.122 ± 0.568
6.265LeuLys: 6.265 ± 0.736
7.914LeuLeu: 7.914 ± 0.971
1.484LeuMet: 1.484 ± 0.295
4.369LeuAsn: 4.369 ± 0.556
3.05LeuPro: 3.05 ± 0.48
3.71LeuGln: 3.71 ± 0.477
4.534LeuArg: 4.534 ± 0.594
5.358LeuSer: 5.358 ± 0.573
4.946LeuThr: 4.946 ± 0.755
5.605LeuVal: 5.605 ± 0.596
0.659LeuTrp: 0.659 ± 0.198
2.968LeuTyr: 2.968 ± 0.384
0.0LeuXaa: 0.0 ± 0.0
Met
2.391MetAla: 2.391 ± 0.559
0.247MetCys: 0.247 ± 0.152
1.978MetAsp: 1.978 ± 0.445
1.072MetGlu: 1.072 ± 0.231
0.989MetPhe: 0.989 ± 0.237
1.319MetGly: 1.319 ± 0.232
0.577MetHis: 0.577 ± 0.166
1.484MetIle: 1.484 ± 0.359
1.814MetLys: 1.814 ± 0.388
2.555MetLeu: 2.555 ± 0.371
0.33MetMet: 0.33 ± 0.129
1.401MetAsn: 1.401 ± 0.388
0.989MetPro: 0.989 ± 0.318
1.566MetGln: 1.566 ± 0.328
1.896MetArg: 1.896 ± 0.392
2.061MetSer: 2.061 ± 0.398
1.814MetThr: 1.814 ± 0.332
0.659MetVal: 0.659 ± 0.219
0.165MetTrp: 0.165 ± 0.138
1.566MetTyr: 1.566 ± 0.306
0.0MetXaa: 0.0 ± 0.0
Asn
4.946AsnAla: 4.946 ± 0.927
0.495AsnCys: 0.495 ± 0.177
1.814AsnAsp: 1.814 ± 0.42
1.896AsnGlu: 1.896 ± 0.373
1.978AsnPhe: 1.978 ± 0.403
4.946AsnGly: 4.946 ± 0.786
0.989AsnHis: 0.989 ± 0.29
3.215AsnIle: 3.215 ± 0.48
2.968AsnLys: 2.968 ± 0.481
3.874AsnLeu: 3.874 ± 0.484
1.649AsnMet: 1.649 ± 0.302
1.896AsnAsn: 1.896 ± 0.436
3.215AsnPro: 3.215 ± 0.535
2.143AsnGln: 2.143 ± 0.495
2.638AsnArg: 2.638 ± 0.557
2.885AsnSer: 2.885 ± 0.462
3.05AsnThr: 3.05 ± 0.457
2.638AsnVal: 2.638 ± 0.39
0.495AsnTrp: 0.495 ± 0.186
2.72AsnTyr: 2.72 ± 0.348
0.0AsnXaa: 0.0 ± 0.0
Pro
2.638ProAla: 2.638 ± 0.604
0.165ProCys: 0.165 ± 0.13
2.473ProAsp: 2.473 ± 0.48
3.38ProGlu: 3.38 ± 0.464
1.154ProPhe: 1.154 ± 0.292
0.0ProGly: 0.0 ± 0.0
0.577ProHis: 0.577 ± 0.178
2.473ProIle: 2.473 ± 0.459
3.05ProLys: 3.05 ± 0.544
2.308ProLeu: 2.308 ± 0.381
0.989ProMet: 0.989 ± 0.313
2.391ProAsn: 2.391 ± 0.387
0.742ProPro: 0.742 ± 0.192
1.731ProGln: 1.731 ± 0.518
1.072ProArg: 1.072 ± 0.306
1.896ProSer: 1.896 ± 0.503
3.297ProThr: 3.297 ± 0.465
2.638ProVal: 2.638 ± 0.527
0.082ProTrp: 0.082 ± 0.084
1.978ProTyr: 1.978 ± 0.324
0.0ProXaa: 0.0 ± 0.0
Gln
4.039GlnAla: 4.039 ± 0.605
0.412GlnCys: 0.412 ± 0.229
1.649GlnAsp: 1.649 ± 0.288
3.05GlnGlu: 3.05 ± 0.515
2.391GlnPhe: 2.391 ± 0.453
3.957GlnGly: 3.957 ± 0.986
1.731GlnHis: 1.731 ± 0.365
2.308GlnIle: 2.308 ± 0.355
2.555GlnLys: 2.555 ± 0.568
4.534GlnLeu: 4.534 ± 0.681
0.989GlnMet: 0.989 ± 0.328
1.814GlnAsn: 1.814 ± 0.449
1.319GlnPro: 1.319 ± 0.32
2.803GlnGln: 2.803 ± 0.535
2.391GlnArg: 2.391 ± 0.415
1.896GlnSer: 1.896 ± 0.301
1.978GlnThr: 1.978 ± 0.423
4.122GlnVal: 4.122 ± 0.599
0.495GlnTrp: 0.495 ± 0.186
2.803GlnTyr: 2.803 ± 0.459
0.0GlnXaa: 0.0 ± 0.0
Arg
3.545ArgAla: 3.545 ± 0.543
0.577ArgCys: 0.577 ± 0.22
2.308ArgAsp: 2.308 ± 0.388
2.885ArgGlu: 2.885 ± 0.62
1.814ArgPhe: 1.814 ± 0.311
3.38ArgGly: 3.38 ± 0.523
0.824ArgHis: 0.824 ± 0.24
2.968ArgIle: 2.968 ± 0.377
3.38ArgLys: 3.38 ± 0.628
4.451ArgLeu: 4.451 ± 0.847
1.814ArgMet: 1.814 ± 0.421
3.38ArgAsn: 3.38 ± 0.656
1.154ArgPro: 1.154 ± 0.319
1.814ArgGln: 1.814 ± 0.422
3.132ArgArg: 3.132 ± 0.668
2.638ArgSer: 2.638 ± 0.426
2.143ArgThr: 2.143 ± 0.384
3.545ArgVal: 3.545 ± 0.494
0.495ArgTrp: 0.495 ± 0.167
1.978ArgTyr: 1.978 ± 0.335
0.0ArgXaa: 0.0 ± 0.0
Ser
5.276SerAla: 5.276 ± 0.675
0.659SerCys: 0.659 ± 0.225
3.627SerAsp: 3.627 ± 0.559
3.297SerGlu: 3.297 ± 0.577
2.72SerPhe: 2.72 ± 0.558
4.287SerGly: 4.287 ± 0.484
1.154SerHis: 1.154 ± 0.306
3.132SerIle: 3.132 ± 0.594
4.534SerLys: 4.534 ± 0.574
3.297SerLeu: 3.297 ± 0.413
1.566SerMet: 1.566 ± 0.329
2.473SerAsn: 2.473 ± 0.57
1.896SerPro: 1.896 ± 0.431
2.391SerGln: 2.391 ± 0.618
2.555SerArg: 2.555 ± 0.474
3.627SerSer: 3.627 ± 0.712
5.193SerThr: 5.193 ± 0.914
2.968SerVal: 2.968 ± 0.518
0.742SerTrp: 0.742 ± 0.292
2.473SerTyr: 2.473 ± 0.438
0.0SerXaa: 0.0 ± 0.0
Thr
4.781ThrAla: 4.781 ± 0.796
0.412ThrCys: 0.412 ± 0.152
3.215ThrAsp: 3.215 ± 0.477
3.215ThrGlu: 3.215 ± 0.425
1.896ThrPhe: 1.896 ± 0.308
7.089ThrGly: 7.089 ± 0.777
0.989ThrHis: 0.989 ± 0.34
2.555ThrIle: 2.555 ± 0.44
3.38ThrLys: 3.38 ± 0.415
4.781ThrLeu: 4.781 ± 0.53
1.319ThrMet: 1.319 ± 0.344
1.896ThrAsn: 1.896 ± 0.439
2.638ThrPro: 2.638 ± 0.478
3.957ThrGln: 3.957 ± 0.727
2.885ThrArg: 2.885 ± 0.53
3.215ThrSer: 3.215 ± 0.63
4.122ThrThr: 4.122 ± 0.674
5.028ThrVal: 5.028 ± 0.934
0.495ThrTrp: 0.495 ± 0.182
2.803ThrTyr: 2.803 ± 0.42
0.0ThrXaa: 0.0 ± 0.0
Val
5.605ValAla: 5.605 ± 0.485
0.742ValCys: 0.742 ± 0.273
4.369ValAsp: 4.369 ± 0.694
3.874ValGlu: 3.874 ± 0.623
2.061ValPhe: 2.061 ± 0.395
5.193ValGly: 5.193 ± 0.54
1.731ValHis: 1.731 ± 0.46
2.968ValIle: 2.968 ± 0.519
4.616ValLys: 4.616 ± 0.585
3.627ValLeu: 3.627 ± 0.535
1.401ValMet: 1.401 ± 0.35
3.957ValAsn: 3.957 ± 0.575
2.968ValPro: 2.968 ± 0.463
3.05ValGln: 3.05 ± 0.526
3.627ValArg: 3.627 ± 0.534
4.616ValSer: 4.616 ± 0.758
4.204ValThr: 4.204 ± 0.667
5.193ValVal: 5.193 ± 0.618
0.495ValTrp: 0.495 ± 0.208
2.391ValTyr: 2.391 ± 0.395
0.0ValXaa: 0.0 ± 0.0
Trp
0.659TrpAla: 0.659 ± 0.221
0.495TrpCys: 0.495 ± 0.194
0.742TrpAsp: 0.742 ± 0.258
0.907TrpGlu: 0.907 ± 0.278
0.742TrpPhe: 0.742 ± 0.252
0.824TrpGly: 0.824 ± 0.233
0.082TrpHis: 0.082 ± 0.079
0.412TrpIle: 0.412 ± 0.151
1.154TrpLys: 1.154 ± 0.22
1.401TrpLeu: 1.401 ± 0.284
0.33TrpMet: 0.33 ± 0.151
0.824TrpAsn: 0.824 ± 0.263
0.0TrpPro: 0.0 ± 0.0
0.33TrpGln: 0.33 ± 0.171
0.659TrpArg: 0.659 ± 0.175
0.577TrpSer: 0.577 ± 0.182
0.824TrpThr: 0.824 ± 0.349
0.824TrpVal: 0.824 ± 0.289
0.33TrpTrp: 0.33 ± 0.16
0.495TrpTyr: 0.495 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.803TyrAla: 2.803 ± 0.458
0.907TyrCys: 0.907 ± 0.319
3.05TyrAsp: 3.05 ± 0.414
2.143TyrGlu: 2.143 ± 0.539
1.154TyrPhe: 1.154 ± 0.281
3.05TyrGly: 3.05 ± 0.476
0.659TyrHis: 0.659 ± 0.237
2.061TyrIle: 2.061 ± 0.455
2.72TyrLys: 2.72 ± 0.505
4.287TyrLeu: 4.287 ± 0.518
1.319TyrMet: 1.319 ± 0.33
2.803TyrAsn: 2.803 ± 0.553
2.061TyrPro: 2.061 ± 0.352
2.473TyrGln: 2.473 ± 0.496
2.308TyrArg: 2.308 ± 0.401
2.555TyrSer: 2.555 ± 0.391
2.061TyrThr: 2.061 ± 0.443
2.555TyrVal: 2.555 ± 0.461
0.247TyrTrp: 0.247 ± 0.122
1.649TyrTyr: 1.649 ± 0.39
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (12132 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski