Nocardia cerradoensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Nocardia

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8308 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A231HE19|A0A231HE19_9NOCA Uncharacterized protein OS=Nocardia cerradoensis OX=85688 GN=B7C42_00221 PE=4 SV=1
MM1 pKa = 8.03CDD3 pKa = 3.02RR4 pKa = 11.84TPRR7 pKa = 11.84QGPRR11 pKa = 11.84SPGFAIAIVAVEE23 pKa = 3.97VVVRR27 pKa = 11.84GRR29 pKa = 11.84FSPGTPPLFRR39 pKa = 11.84GDD41 pKa = 3.61SSPTLTGPSVRR52 pKa = 11.84SVCPMVGLTKK62 pKa = 10.58SLALPTSAQPGGDD75 pKa = 3.33TPGWSGVVGVSVFGIGPVIPGWPSWSVSGGADD107 pKa = 3.26GSGGTGAGGGAGVCDD122 pKa = 3.9WGGADD127 pKa = 4.36ADD129 pKa = 4.11GSGDD133 pKa = 3.63CGAGGGVGVGDD144 pKa = 3.58WSGGANVDD152 pKa = 4.46GPGDD156 pKa = 3.73CAAGGGVGVGDD167 pKa = 3.54WGGGADD173 pKa = 3.74ADD175 pKa = 4.41GSGEE179 pKa = 4.15CGAGMSEE186 pKa = 4.24GGAAGAAGAGVTGGAACGSDD206 pKa = 3.1VGGAGGGTEE215 pKa = 4.62LSGPDD220 pKa = 3.55GAGGCVGSGEE230 pKa = 4.25VGAEE234 pKa = 3.92EE235 pKa = 4.08VGGAGVGAGPGSCVGAPGAGADD257 pKa = 3.92EE258 pKa = 5.13LGAGSPGVGAGCVWGGAGAGLAGAGAGAGAGAGAGAGAGGDD299 pKa = 3.71CGVAGVPGTVAEE311 pKa = 4.37LGAWSGVAGSDD322 pKa = 3.58SDD324 pKa = 4.74DD325 pKa = 3.79GGAVGLWSACGRR337 pKa = 11.84SPFGRR342 pKa = 11.84AGALGRR348 pKa = 11.84GPAGSACAGGSGSAGTAVPAAEE370 pKa = 4.01

Molecular weight: 32.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A231H4K3|A0A231H4K3_9NOCA DUF4189 domain-containing protein OS=Nocardia cerradoensis OX=85688 GN=B7C42_04514 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8308

0

8308

2632396

29

14660

316.9

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.493 ± 0.037

0.79 ± 0.009

6.28 ± 0.025

5.401 ± 0.022

2.898 ± 0.016

8.927 ± 0.035

2.342 ± 0.014

4.137 ± 0.017

1.849 ± 0.02

9.773 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.889 ± 0.011

1.981 ± 0.014

5.985 ± 0.028

2.886 ± 0.018

7.994 ± 0.032

5.247 ± 0.019

6.144 ± 0.024

8.428 ± 0.031

1.478 ± 0.012

2.075 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski