Aeromonas phage phiA8-29

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; Tedavirus; Aeromonas virus A8-29

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6DYA4|A0A1W6DYA4_9CAUD Uncharacterized protein OS=Aeromonas phage phiA8-29 OX=1978922 GN=phiA829_177 PE=4 SV=1
MM1 pKa = 7.7LPNQYY6 pKa = 10.29LQGGPAVGSAPDD18 pKa = 3.53TTIPSVIIQEE28 pKa = 4.24LVNAVDD34 pKa = 4.96DD35 pKa = 4.59LLPEE39 pKa = 4.49DD40 pKa = 4.1FEE42 pKa = 5.57LYY44 pKa = 10.3EE45 pKa = 4.22LEE47 pKa = 4.89GDD49 pKa = 3.36QSVYY53 pKa = 10.47FNYY56 pKa = 10.71AEE58 pKa = 4.29RR59 pKa = 11.84QDD61 pKa = 3.53EE62 pKa = 4.24ATAEE66 pKa = 3.95LRR68 pKa = 11.84YY69 pKa = 6.83TTARR73 pKa = 11.84EE74 pKa = 3.56KK75 pKa = 11.05GYY77 pKa = 9.02TEE79 pKa = 5.99DD80 pKa = 3.22IGPRR84 pKa = 11.84YY85 pKa = 9.59ALSIIVDD92 pKa = 4.11GPDD95 pKa = 3.76PDD97 pKa = 5.68NKK99 pKa = 8.35TTWFTLEE106 pKa = 3.92EE107 pKa = 4.12AVDD110 pKa = 3.83YY111 pKa = 10.62AVKK114 pKa = 10.37QIRR117 pKa = 11.84RR118 pKa = 11.84VLRR121 pKa = 11.84VAEE124 pKa = 4.13

Molecular weight: 13.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6DXS8|A0A1W6DXS8_9CAUD Putative tail fiber protein OS=Aeromonas phage phiA8-29 OX=1978922 GN=phiA829_185 PE=4 SV=1
MM1 pKa = 7.44SKK3 pKa = 9.67EE4 pKa = 4.08CKK6 pKa = 9.52MPSLFEE12 pKa = 4.28GDD14 pKa = 3.0IVQLDD19 pKa = 3.44EE20 pKa = 4.55AYY22 pKa = 10.57RR23 pKa = 11.84IRR25 pKa = 11.84VNAKK29 pKa = 8.84GEE31 pKa = 3.74KK32 pKa = 9.7RR33 pKa = 11.84KK34 pKa = 9.75IKK36 pKa = 9.72RR37 pKa = 11.84CPMGYY42 pKa = 9.96KK43 pKa = 10.06PGPDD47 pKa = 3.19GQSCVKK53 pKa = 10.38IGAQEE58 pKa = 4.06KK59 pKa = 9.78ISRR62 pKa = 11.84KK63 pKa = 9.59KK64 pKa = 10.23GAIRR68 pKa = 11.84GNRR71 pKa = 11.84KK72 pKa = 9.17KK73 pKa = 10.43VGQWKK78 pKa = 10.12RR79 pKa = 11.84IEE81 pKa = 4.13RR82 pKa = 11.84KK83 pKa = 9.26KK84 pKa = 10.33KK85 pKa = 9.35KK86 pKa = 10.48AMARR90 pKa = 11.84RR91 pKa = 11.84KK92 pKa = 10.05QYY94 pKa = 11.37GLL96 pKa = 3.44

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

185

0

185

45227

28

1580

244.5

27.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.642 ± 0.216

1.218 ± 0.083

5.892 ± 0.129

7.462 ± 0.188

4.681 ± 0.121

6.75 ± 0.161

1.775 ± 0.083

5.689 ± 0.118

7.025 ± 0.212

8.382 ± 0.141

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.084

4.584 ± 0.128

3.858 ± 0.112

3.653 ± 0.115

5.035 ± 0.152

6.938 ± 0.148

5.651 ± 0.22

7.308 ± 0.166

1.512 ± 0.075

3.252 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski