Thioclava arenosa

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Thioclava

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A4CK52|A0A2A4CK52_9RHOB Proline iminopeptidase OS=Thioclava arenosa OX=1795308 GN=pip PE=3 SV=1
MM1 pKa = 7.22IVAAVAGCSGSAVIKK16 pKa = 10.98SGDD19 pKa = 3.55DD20 pKa = 3.13TGGGDD25 pKa = 4.94IITLPGDD32 pKa = 3.6TTSDD36 pKa = 3.38GSVSRR41 pKa = 11.84YY42 pKa = 8.49EE43 pKa = 4.32PEE45 pKa = 4.26DD46 pKa = 3.47AASGSGYY53 pKa = 10.25AQAVEE58 pKa = 4.17VRR60 pKa = 11.84DD61 pKa = 3.98NGDD64 pKa = 3.05GTTTFLVDD72 pKa = 3.27NLAFDD77 pKa = 3.78GAEE80 pKa = 3.98YY81 pKa = 11.05SEE83 pKa = 4.39GTVVSSLGRR92 pKa = 11.84TGINAGPFSVYY103 pKa = 9.9EE104 pKa = 4.27GPEE107 pKa = 3.93FEE109 pKa = 5.02PDD111 pKa = 3.64FVTGTAIAQLTHH123 pKa = 6.32RR124 pKa = 11.84AIYY127 pKa = 10.21AKK129 pKa = 9.13STSGNSEE136 pKa = 3.72FAIVRR141 pKa = 11.84TGSYY145 pKa = 8.82TGYY148 pKa = 10.76GFGGFLYY155 pKa = 10.61SRR157 pKa = 11.84TGSVTLPATATGTGISRR174 pKa = 11.84QASYY178 pKa = 11.05SGDD181 pKa = 3.47YY182 pKa = 10.89AGLIDD187 pKa = 3.79YY188 pKa = 10.02NGRR191 pKa = 11.84SGLDD195 pKa = 3.43YY196 pKa = 10.7VTGTASIAIDD206 pKa = 3.75FADD209 pKa = 4.51FDD211 pKa = 5.01DD212 pKa = 4.93GAAIYY217 pKa = 10.39GSISDD222 pKa = 4.14RR223 pKa = 11.84RR224 pKa = 11.84MFDD227 pKa = 2.66IDD229 pKa = 4.67GNDD232 pKa = 2.98ISQDD236 pKa = 3.33YY237 pKa = 10.87LDD239 pKa = 4.78RR240 pKa = 11.84LNEE243 pKa = 4.03EE244 pKa = 4.17LDD246 pKa = 3.76YY247 pKa = 10.73PAGSGYY253 pKa = 9.43TSLPTLVFDD262 pKa = 4.79IGPGVLADD270 pKa = 3.49NGEE273 pKa = 4.18IEE275 pKa = 4.41GTLGVDD281 pKa = 3.34VLTAGGVVQEE291 pKa = 4.44LVSGKK296 pKa = 10.15YY297 pKa = 9.87YY298 pKa = 10.99GVISGDD304 pKa = 3.35VTAGTDD310 pKa = 3.36EE311 pKa = 4.3VVGVIVVTGDD321 pKa = 3.1DD322 pKa = 3.58PGYY325 pKa = 10.86DD326 pKa = 3.15GVTMRR331 pKa = 11.84EE332 pKa = 3.6TGGFIVYY339 pKa = 9.79RR340 pKa = 11.84QQ341 pKa = 3.16

Molecular weight: 35.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A4CP57|A0A2A4CP57_9RHOB Periplasmic nitrate reductase OS=Thioclava arenosa OX=1795308 GN=napA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.42GGQKK29 pKa = 9.85VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2673

0

2673

840682

37

2492

314.5

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.208 ± 0.079

0.857 ± 0.015

5.514 ± 0.036

6.477 ± 0.047

3.685 ± 0.026

8.878 ± 0.048

1.971 ± 0.02

5.074 ± 0.03

3.198 ± 0.041

10.401 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.744 ± 0.023

2.334 ± 0.023

5.326 ± 0.041

2.991 ± 0.023

6.942 ± 0.048

4.783 ± 0.032

5.032 ± 0.035

7.088 ± 0.037

1.372 ± 0.021

2.125 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski