Staphylococcus virus IPLA5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rockefellervirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6TG15|I6TG15_9CAUD Uncharacterized protein OS=Staphylococcus virus IPLA5 OX=1197952 GN=IPLA5_0064 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 4.02NMKK5 pKa = 10.08FRR7 pKa = 11.84AWDD10 pKa = 3.33KK11 pKa = 11.31DD12 pKa = 3.3EE13 pKa = 5.23RR14 pKa = 11.84AMLDD18 pKa = 3.1VHH20 pKa = 7.07GINFDD25 pKa = 3.68AQGIWTNEE33 pKa = 4.2LIDD36 pKa = 5.29DD37 pKa = 4.33EE38 pKa = 5.32SDD40 pKa = 3.46GNFIFLDD47 pKa = 4.12DD48 pKa = 4.06VVLMQSTGLRR58 pKa = 11.84DD59 pKa = 3.4INGTEE64 pKa = 3.88IYY66 pKa = 10.21EE67 pKa = 4.22GDD69 pKa = 3.0IVRR72 pKa = 11.84YY73 pKa = 9.37NRR75 pKa = 11.84GISWSVEE82 pKa = 3.43KK83 pKa = 10.63FPYY86 pKa = 9.09VVKK89 pKa = 11.03NSMEE93 pKa = 4.06GFVFEE98 pKa = 4.78YY99 pKa = 11.21GLIQHH104 pKa = 6.3SLSKK108 pKa = 10.33KK109 pKa = 8.65IEE111 pKa = 4.04YY112 pKa = 8.39VTVIGNIYY120 pKa = 9.62EE121 pKa = 4.16NPEE124 pKa = 4.08LLEE127 pKa = 4.78DD128 pKa = 3.69NN129 pKa = 4.56

Molecular weight: 15.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6SML9|I6SML9_9CAUD BppU_N domain-containing protein OS=Staphylococcus virus IPLA5 OX=1197952 GN=IPLA5_0020 PE=4 SV=1
MM1 pKa = 7.54SLTPNEE7 pKa = 3.89WKK9 pKa = 10.31DD10 pKa = 3.28WVVGRR15 pKa = 11.84RR16 pKa = 11.84LALLDD21 pKa = 3.78EE22 pKa = 4.42QEE24 pKa = 4.32TLLFGAQANGLVQAGKK40 pKa = 9.58SLKK43 pKa = 10.18RR44 pKa = 11.84LQKK47 pKa = 8.59QLEE50 pKa = 3.97RR51 pKa = 11.84ARR53 pKa = 11.84YY54 pKa = 6.86EE55 pKa = 3.64VRR57 pKa = 11.84GQSEE61 pKa = 4.38EE62 pKa = 4.06YY63 pKa = 10.73EE64 pKa = 4.03RR65 pKa = 11.84MKK67 pKa = 10.59EE68 pKa = 4.04RR69 pKa = 11.84KK70 pKa = 8.73LAHH73 pKa = 6.28NKK75 pKa = 10.13RR76 pKa = 11.84IRR78 pKa = 11.84NVQKK82 pKa = 9.93QGTRR86 pKa = 11.84RR87 pKa = 11.84FMNSLRR93 pKa = 11.84NTSQKK98 pKa = 11.01GGG100 pKa = 3.49

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13137

46

1034

199.0

22.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.465 ± 0.489

0.502 ± 0.099

6.348 ± 0.345

7.102 ± 0.631

3.692 ± 0.184

6.143 ± 0.38

1.713 ± 0.163

7.323 ± 0.243

9.249 ± 0.476

7.62 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.166

6.851 ± 0.314

2.725 ± 0.192

4.194 ± 0.208

4.324 ± 0.222

6.211 ± 0.316

6.326 ± 0.231

5.975 ± 0.25

1.302 ± 0.161

4.308 ± 0.355
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski