Amino acid dipepetide frequency for Alicyclobacillus macrosporangiidus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.995AlaAla: 13.995 ± 0.186	1.163AlaCys: 1.163 ± 0.038	5.812AlaAsp: 5.812 ± 0.083	7.016AlaGlu: 7.016 ± 0.095	3.657AlaPhe: 3.657 ± 0.06	10.268AlaGly: 10.268 ± 0.139	2.315AlaHis: 2.315 ± 0.053	5.221AlaIle: 5.221 ± 0.078	3.374AlaLys: 3.374 ± 0.067	11.769AlaLeu: 11.769 ± 0.125	2.904AlaMet: 2.904 ± 0.054	2.373AlaAsn: 2.373 ± 0.048	4.454AlaPro: 4.454 ± 0.071	4.056AlaGln: 4.056 ± 0.079	7.628AlaArg: 7.628 ± 0.106	5.374AlaSer: 5.374 ± 0.078	4.666AlaThr: 4.666 ± 0.07	9.542AlaVal: 9.542 ± 0.122	1.881AlaTrp: 1.881 ± 0.048	2.91AlaTyr: 2.91 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.011CysAla: 1.011 ± 0.03	0.128CysCys: 0.128 ± 0.014	0.543CysAsp: 0.543 ± 0.024	0.51CysGlu: 0.51 ± 0.018	0.304CysPhe: 0.304 ± 0.021	1.099CysGly: 1.099 ± 0.035	0.252CysHis: 0.252 ± 0.016	0.445CysIle: 0.445 ± 0.021	0.248CysLys: 0.248 ± 0.015	0.87CysLeu: 0.87 ± 0.026	0.179CysMet: 0.179 ± 0.013	0.219CysAsn: 0.219 ± 0.015	0.553CysPro: 0.553 ± 0.025	0.288CysGln: 0.288 ± 0.018	0.739CysArg: 0.739 ± 0.025	0.438CysSer: 0.438 ± 0.022	0.548CysThr: 0.548 ± 0.021	0.719CysVal: 0.719 ± 0.028	0.113CysTrp: 0.113 ± 0.01	0.199CysTyr: 0.199 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	5.884AspAla: 5.884 ± 0.09	0.373AspCys: 0.373 ± 0.02	2.562AspAsp: 2.562 ± 0.06	3.737AspGlu: 3.737 ± 0.066	1.655AspPhe: 1.655 ± 0.041	4.834AspGly: 4.834 ± 0.079	1.182AspHis: 1.182 ± 0.031	2.593AspIle: 2.593 ± 0.051	1.515AspLys: 1.515 ± 0.043	5.174AspLeu: 5.174 ± 0.08	1.066AspMet: 1.066 ± 0.03	1.016AspAsn: 1.016 ± 0.034	3.327AspPro: 3.327 ± 0.052	1.545AspGln: 1.545 ± 0.05	3.687AspArg: 3.687 ± 0.06	1.903AspSer: 1.903 ± 0.045	2.474AspThr: 2.474 ± 0.048	4.91AspVal: 4.91 ± 0.071	0.914AspTrp: 0.914 ± 0.029	1.237AspTyr: 1.237 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	7.349GluAla: 7.349 ± 0.105	0.366GluCys: 0.366 ± 0.018	2.942GluAsp: 2.942 ± 0.06	4.167GluGlu: 4.167 ± 0.09	1.696GluPhe: 1.696 ± 0.041	4.008GluGly: 4.008 ± 0.062	1.475GluHis: 1.475 ± 0.034	2.972GluIle: 2.972 ± 0.057	1.939GluLys: 1.939 ± 0.052	5.498GluLeu: 5.498 ± 0.082	1.581GluMet: 1.581 ± 0.046	1.359GluAsn: 1.359 ± 0.037	2.563GluPro: 2.563 ± 0.053	2.682GluGln: 2.682 ± 0.056	5.821GluArg: 5.821 ± 0.092	2.492GluSer: 2.492 ± 0.056	2.978GluThr: 2.978 ± 0.058	4.969GluVal: 4.969 ± 0.073	0.996GluTrp: 0.996 ± 0.033	1.381GluTyr: 1.381 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.688PheAla: 3.688 ± 0.066	0.396PheCys: 0.396 ± 0.019	1.993PheAsp: 1.993 ± 0.042	1.776PheGlu: 1.776 ± 0.04	1.317PhePhe: 1.317 ± 0.034	3.321PheGly: 3.321 ± 0.063	0.908PheHis: 0.908 ± 0.028	1.662PheIle: 1.662 ± 0.043	0.753PheLys: 0.753 ± 0.03	3.556PheLeu: 3.556 ± 0.063	0.632PheMet: 0.632 ± 0.02	0.868PheAsn: 0.868 ± 0.029	1.616PhePro: 1.616 ± 0.043	1.225PheGln: 1.225 ± 0.038	2.281PheArg: 2.281 ± 0.05	1.952PheSer: 1.952 ± 0.044	2.097PheThr: 2.097 ± 0.045	3.051PheVal: 3.051 ± 0.062	0.523PheTrp: 0.523 ± 0.024	0.934PheTyr: 0.934 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	8.19GlyAla: 8.19 ± 0.119	0.947GlyCys: 0.947 ± 0.035	3.958GlyAsp: 3.958 ± 0.064	4.403GlyGlu: 4.403 ± 0.065	3.149GlyPhe: 3.149 ± 0.057	7.14GlyGly: 7.14 ± 0.108	1.878GlyHis: 1.878 ± 0.04	4.547GlyIle: 4.547 ± 0.07	2.919GlyLys: 2.919 ± 0.048	8.281GlyLeu: 8.281 ± 0.098	2.494GlyMet: 2.494 ± 0.052	1.941GlyAsn: 1.941 ± 0.054	3.43GlyPro: 3.43 ± 0.065	3.196GlyGln: 3.196 ± 0.064	6.16GlyArg: 6.16 ± 0.08	4.23GlySer: 4.23 ± 0.067	4.78GlyThr: 4.78 ± 0.081	7.616GlyVal: 7.616 ± 0.086	1.66GlyTrp: 1.66 ± 0.045	2.65GlyTyr: 2.65 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	2.362HisAla: 2.362 ± 0.045	0.234HisCys: 0.234 ± 0.014	1.094HisAsp: 1.094 ± 0.031	1.252HisGlu: 1.252 ± 0.034	0.846HisPhe: 0.846 ± 0.033	2.055HisGly: 2.055 ± 0.047	0.795HisHis: 0.795 ± 0.033	1.056HisIle: 1.056 ± 0.028	0.551HisLys: 0.551 ± 0.023	2.682HisLeu: 2.682 ± 0.051	0.514HisMet: 0.514 ± 0.023	0.528HisAsn: 0.528 ± 0.021	1.933HisPro: 1.933 ± 0.04	0.861HisGln: 0.861 ± 0.032	2.015HisArg: 2.015 ± 0.044	0.919HisSer: 0.919 ± 0.03	1.275HisThr: 1.275 ± 0.035	2.079HisVal: 2.079 ± 0.048	0.432HisTrp: 0.432 ± 0.02	0.636HisTyr: 0.636 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.429IleAla: 5.429 ± 0.072	0.473IleCys: 0.473 ± 0.022	2.696IleAsp: 2.696 ± 0.059	2.775IleGlu: 2.775 ± 0.053	1.242IlePhe: 1.242 ± 0.038	4.449IleGly: 4.449 ± 0.072	1.321IleHis: 1.321 ± 0.035	1.875IleIle: 1.875 ± 0.053	0.979IleLys: 0.979 ± 0.034	4.711IleLeu: 4.711 ± 0.076	0.704IleMet: 0.704 ± 0.026	1.133IleAsn: 1.133 ± 0.035	2.914IlePro: 2.914 ± 0.053	1.927IleGln: 1.927 ± 0.048	3.909IleArg: 3.909 ± 0.061	2.29IleSer: 2.29 ± 0.048	2.579IleThr: 2.579 ± 0.052	3.988IleVal: 3.988 ± 0.073	0.578IleTrp: 0.578 ± 0.023	1.088IleTyr: 1.088 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.035LysAla: 3.035 ± 0.059	0.165LysCys: 0.165 ± 0.014	1.53LysAsp: 1.53 ± 0.043	1.843LysGlu: 1.843 ± 0.048	0.838LysPhe: 0.838 ± 0.029	2.083LysGly: 2.083 ± 0.057	0.677LysHis: 0.677 ± 0.026	1.335LysIle: 1.335 ± 0.037	1.158LysLys: 1.158 ± 0.048	2.708LysLeu: 2.708 ± 0.06	0.795LysMet: 0.795 ± 0.028	0.783LysAsn: 0.783 ± 0.028	1.712LysPro: 1.712 ± 0.042	1.236LysGln: 1.236 ± 0.037	2.471LysArg: 2.471 ± 0.052	1.583LysSer: 1.583 ± 0.041	1.749LysThr: 1.749 ± 0.042	2.493LysVal: 2.493 ± 0.052	0.441LysTrp: 0.441 ± 0.019	0.805LysTyr: 0.805 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	12.223LeuAla: 12.223 ± 0.123	1.097LeuCys: 1.097 ± 0.032	5.076LeuAsp: 5.076 ± 0.085	5.88LeuGlu: 5.88 ± 0.102	3.614LeuPhe: 3.614 ± 0.068	7.919LeuGly: 7.919 ± 0.094	2.438LeuHis: 2.438 ± 0.043	4.128LeuIle: 4.128 ± 0.073	2.855LeuLys: 2.855 ± 0.06	9.938LeuLeu: 9.938 ± 0.136	2.306LeuMet: 2.306 ± 0.052	2.483LeuAsn: 2.483 ± 0.043	5.372LeuPro: 5.372 ± 0.075	4.018LeuGln: 4.018 ± 0.077	7.81LeuArg: 7.81 ± 0.083	5.67LeuSer: 5.67 ± 0.083	5.671LeuThr: 5.671 ± 0.084	8.219LeuVal: 8.219 ± 0.094	1.556LeuTrp: 1.556 ± 0.042	2.734LeuTyr: 2.734 ± 0.05	0.0LeuXaa: 0.0 ± 0.0
Met	2.941MetAla: 2.941 ± 0.055	0.183MetCys: 0.183 ± 0.013	1.431MetAsp: 1.431 ± 0.038	1.467MetGlu: 1.467 ± 0.038	0.77MetPhe: 0.77 ± 0.028	1.845MetGly: 1.845 ± 0.038	0.551MetHis: 0.551 ± 0.024	1.033MetIle: 1.033 ± 0.03	0.958MetLys: 0.958 ± 0.03	2.166MetLeu: 2.166 ± 0.049	0.719MetMet: 0.719 ± 0.028	0.802MetAsn: 0.802 ± 0.03	1.205MetPro: 1.205 ± 0.035	1.002MetGln: 1.002 ± 0.032	1.804MetArg: 1.804 ± 0.041	1.421MetSer: 1.421 ± 0.035	1.444MetThr: 1.444 ± 0.039	1.973MetVal: 1.973 ± 0.043	0.313MetTrp: 0.313 ± 0.019	0.589MetTyr: 0.589 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.581AsnAla: 2.581 ± 0.054	0.194AsnCys: 0.194 ± 0.015	1.062AsnAsp: 1.062 ± 0.037	1.195AsnGlu: 1.195 ± 0.033	0.797AsnPhe: 0.797 ± 0.027	2.199AsnGly: 2.199 ± 0.054	0.577AsnHis: 0.577 ± 0.022	1.229AsnIle: 1.229 ± 0.037	0.687AsnLys: 0.687 ± 0.027	2.508AsnLeu: 2.508 ± 0.053	0.53AsnMet: 0.53 ± 0.021	0.611AsnAsn: 0.611 ± 0.03	1.929AsnPro: 1.929 ± 0.046	1.054AsnGln: 1.054 ± 0.039	1.798AsnArg: 1.798 ± 0.044	1.019AsnSer: 1.019 ± 0.042	1.403AsnThr: 1.403 ± 0.039	2.028AsnVal: 2.028 ± 0.046	0.402AsnTrp: 0.402 ± 0.02	0.602AsnTyr: 0.602 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	5.616ProAla: 5.616 ± 0.094	0.377ProCys: 0.377 ± 0.021	3.545ProAsp: 3.545 ± 0.061	3.811ProGlu: 3.811 ± 0.056	1.878ProPhe: 1.878 ± 0.035	4.966ProGly: 4.966 ± 0.075	1.237ProHis: 1.237 ± 0.033	1.985ProIle: 1.985 ± 0.041	1.543ProLys: 1.543 ± 0.043	4.672ProLeu: 4.672 ± 0.069	1.167ProMet: 1.167 ± 0.031	1.443ProAsn: 1.443 ± 0.039	2.335ProPro: 2.335 ± 0.05	1.702ProGln: 1.702 ± 0.04	2.974ProArg: 2.974 ± 0.06	2.754ProSer: 2.754 ± 0.062	2.214ProThr: 2.214 ± 0.044	5.127ProVal: 5.127 ± 0.073	0.857ProTrp: 0.857 ± 0.036	1.508ProTyr: 1.508 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	4.914GlnAla: 4.914 ± 0.089	0.243GlnCys: 0.243 ± 0.016	1.649GlnAsp: 1.649 ± 0.039	2.196GlnGlu: 2.196 ± 0.048	1.207GlnPhe: 1.207 ± 0.033	2.678GlnGly: 2.678 ± 0.061	0.906GlnHis: 0.906 ± 0.031	1.706GlnIle: 1.706 ± 0.043	1.166GlnLys: 1.166 ± 0.039	3.529GlnLeu: 3.529 ± 0.068	1.099GlnMet: 1.099 ± 0.036	0.959GlnAsn: 0.959 ± 0.037	1.957GlnPro: 1.957 ± 0.05	1.765GlnGln: 1.765 ± 0.053	2.99GlnArg: 2.99 ± 0.057	1.905GlnSer: 1.905 ± 0.048	2.142GlnThr: 2.142 ± 0.058	3.648GlnVal: 3.648 ± 0.063	0.636GlnTrp: 0.636 ± 0.026	1.019GlnTyr: 1.019 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	7.383ArgAla: 7.383 ± 0.089	0.694ArgCys: 0.694 ± 0.03	3.759ArgAsp: 3.759 ± 0.061	4.813ArgGlu: 4.813 ± 0.077	2.837ArgPhe: 2.837 ± 0.046	5.022ArgGly: 5.022 ± 0.076	1.962ArgHis: 1.962 ± 0.04	3.844ArgIle: 3.844 ± 0.066	2.342ArgLys: 2.342 ± 0.05	8.184ArgLeu: 8.184 ± 0.107	2.267ArgMet: 2.267 ± 0.044	1.683ArgAsn: 1.683 ± 0.037	3.844ArgPro: 3.844 ± 0.068	3.176ArgGln: 3.176 ± 0.06	7.055ArgArg: 7.055 ± 0.107	3.176ArgSer: 3.176 ± 0.057	3.927ArgThr: 3.927 ± 0.06	6.408ArgVal: 6.408 ± 0.083	1.527ArgTrp: 1.527 ± 0.046	2.179ArgTyr: 2.179 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	5.262SerAla: 5.262 ± 0.075	0.42SerCys: 0.42 ± 0.022	2.45SerAsp: 2.45 ± 0.047	2.481SerGlu: 2.481 ± 0.051	1.884SerPhe: 1.884 ± 0.042	5.041SerGly: 5.041 ± 0.092	1.085SerHis: 1.085 ± 0.028	2.389SerIle: 2.389 ± 0.048	1.377SerLys: 1.377 ± 0.029	4.888SerLeu: 4.888 ± 0.072	1.295SerMet: 1.295 ± 0.039	1.277SerAsn: 1.277 ± 0.042	2.686SerPro: 2.686 ± 0.06	1.73SerGln: 1.73 ± 0.049	3.346SerArg: 3.346 ± 0.056	2.732SerSer: 2.732 ± 0.073	2.555SerThr: 2.555 ± 0.056	4.393SerVal: 4.393 ± 0.07	0.72SerTrp: 0.72 ± 0.026	1.355SerTyr: 1.355 ± 0.039	0.0SerXaa: 0.0 ± 0.0
Thr	5.593ThrAla: 5.593 ± 0.085	0.465ThrCys: 0.465 ± 0.023	2.633ThrAsp: 2.633 ± 0.059	2.672ThrGlu: 2.672 ± 0.054	1.93ThrPhe: 1.93 ± 0.05	5.294ThrGly: 5.294 ± 0.072	1.226ThrHis: 1.226 ± 0.035	2.475ThrIle: 2.475 ± 0.052	1.384ThrLys: 1.384 ± 0.04	5.645ThrLeu: 5.645 ± 0.064	1.093ThrMet: 1.093 ± 0.032	1.322ThrAsn: 1.322 ± 0.045	3.136ThrPro: 3.136 ± 0.06	1.659ThrGln: 1.659 ± 0.043	3.203ThrArg: 3.203 ± 0.05	2.708ThrSer: 2.708 ± 0.061	2.774ThrThr: 2.774 ± 0.062	5.293ThrVal: 5.293 ± 0.087	0.892ThrTrp: 0.892 ± 0.035	1.536ThrTyr: 1.536 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	8.924ValAla: 8.924 ± 0.103	1.021ValCys: 1.021 ± 0.03	4.613ValAsp: 4.613 ± 0.063	4.879ValGlu: 4.879 ± 0.075	3.293ValPhe: 3.293 ± 0.062	5.866ValGly: 5.866 ± 0.086	2.25ValHis: 2.25 ± 0.049	4.532ValIle: 4.532 ± 0.074	2.527ValLys: 2.527 ± 0.053	9.271ValLeu: 9.271 ± 0.099	2.162ValMet: 2.162 ± 0.042	2.312ValAsn: 2.312 ± 0.04	4.565ValPro: 4.565 ± 0.061	3.413ValGln: 3.413 ± 0.06	6.733ValArg: 6.733 ± 0.086	4.779ValSer: 4.779 ± 0.071	5.096ValThr: 5.096 ± 0.08	8.076ValVal: 8.076 ± 0.1	1.473ValTrp: 1.473 ± 0.04	2.553ValTyr: 2.553 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	1.449TrpAla: 1.449 ± 0.038	0.188TrpCys: 0.188 ± 0.014	0.726TrpAsp: 0.726 ± 0.027	0.767TrpGlu: 0.767 ± 0.028	0.664TrpPhe: 0.664 ± 0.025	1.011TrpGly: 1.011 ± 0.038	0.376TrpHis: 0.376 ± 0.019	0.829TrpIle: 0.829 ± 0.03	0.488TrpLys: 0.488 ± 0.023	2.055TrpLeu: 2.055 ± 0.056	0.6TrpMet: 0.6 ± 0.027	0.523TrpAsn: 0.523 ± 0.021	0.779TrpPro: 0.779 ± 0.029	0.685TrpGln: 0.685 ± 0.025	1.415TrpArg: 1.415 ± 0.04	0.856TrpSer: 0.856 ± 0.031	1.023TrpThr: 1.023 ± 0.039	1.443TrpVal: 1.443 ± 0.044	0.411TrpTrp: 0.411 ± 0.021	0.438TrpTyr: 0.438 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.552TyrAla: 2.552 ± 0.049	0.276TyrCys: 0.276 ± 0.018	1.517TyrAsp: 1.517 ± 0.041	1.594TyrGlu: 1.594 ± 0.04	0.969TyrPhe: 0.969 ± 0.03	2.471TyrGly: 2.471 ± 0.047	0.71TyrHis: 0.71 ± 0.025	1.205TyrIle: 1.205 ± 0.036	0.677TyrLys: 0.677 ± 0.03	2.9TyrLeu: 2.9 ± 0.049	0.507TyrMet: 0.507 ± 0.022	0.77TyrAsn: 0.77 ± 0.031	1.404TyrPro: 1.404 ± 0.037	1.094TyrGln: 1.094 ± 0.038	2.244TyrArg: 2.244 ± 0.048	1.164TyrSer: 1.164 ± 0.032	1.534TyrThr: 1.534 ± 0.039	2.362TyrVal: 2.362 ± 0.048	0.401TyrTrp: 0.401 ± 0.02	0.805TyrTyr: 0.805 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3795 proteins (1092489 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---