Amino acid dipepetide frequency for Erythrobacter aureus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.667AlaAla: 15.667 ± 0.181	1.116AlaCys: 1.116 ± 0.033	7.114AlaAsp: 7.114 ± 0.079	8.334AlaGlu: 8.334 ± 0.12	4.179AlaPhe: 4.179 ± 0.076	10.014AlaGly: 10.014 ± 0.13	2.238AlaHis: 2.238 ± 0.052	6.867AlaIle: 6.867 ± 0.085	4.582AlaLys: 4.582 ± 0.076	12.832AlaLeu: 12.832 ± 0.135	3.847AlaMet: 3.847 ± 0.058	3.194AlaAsn: 3.194 ± 0.056	5.343AlaPro: 5.343 ± 0.074	4.242AlaGln: 4.242 ± 0.064	8.953AlaArg: 8.953 ± 0.116	6.631AlaSer: 6.631 ± 0.091	5.691AlaThr: 5.691 ± 0.084	7.635AlaVal: 7.635 ± 0.103	1.48AlaTrp: 1.48 ± 0.035	2.537AlaTyr: 2.537 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.035CysAla: 1.035 ± 0.038	0.087CysCys: 0.087 ± 0.01	0.627CysAsp: 0.627 ± 0.032	0.542CysGlu: 0.542 ± 0.022	0.296CysPhe: 0.296 ± 0.018	0.861CysGly: 0.861 ± 0.028	0.218CysHis: 0.218 ± 0.013	0.403CysIle: 0.403 ± 0.02	0.231CysLys: 0.231 ± 0.014	0.712CysLeu: 0.712 ± 0.027	0.162CysMet: 0.162 ± 0.012	0.237CysAsn: 0.237 ± 0.017	0.433CysPro: 0.433 ± 0.022	0.245CysGln: 0.245 ± 0.017	0.551CysArg: 0.551 ± 0.02	0.53CysSer: 0.53 ± 0.026	0.441CysThr: 0.441 ± 0.02	0.593CysVal: 0.593 ± 0.027	0.121CysTrp: 0.121 ± 0.011	0.189CysTyr: 0.189 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.317AspAla: 7.317 ± 0.071	0.573AspCys: 0.573 ± 0.025	3.795AspAsp: 3.795 ± 0.072	4.513AspGlu: 4.513 ± 0.074	2.422AspPhe: 2.422 ± 0.055	5.911AspGly: 5.911 ± 0.085	1.359AspHis: 1.359 ± 0.043	3.268AspIle: 3.268 ± 0.059	2.014AspLys: 2.014 ± 0.051	6.026AspLeu: 6.026 ± 0.086	1.612AspMet: 1.612 ± 0.043	1.59AspAsn: 1.59 ± 0.04	3.793AspPro: 3.793 ± 0.07	1.789AspGln: 1.789 ± 0.047	4.908AspArg: 4.908 ± 0.077	2.531AspSer: 2.531 ± 0.049	3.02AspThr: 3.02 ± 0.057	3.866AspVal: 3.866 ± 0.07	1.177AspTrp: 1.177 ± 0.035	1.646AspTyr: 1.646 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	8.452GluAla: 8.452 ± 0.121	0.44GluCys: 0.44 ± 0.022	3.947GluAsp: 3.947 ± 0.077	4.57GluGlu: 4.57 ± 0.095	2.149GluPhe: 2.149 ± 0.042	5.311GluGly: 5.311 ± 0.079	1.361GluHis: 1.361 ± 0.036	3.534GluIle: 3.534 ± 0.062	2.63GluLys: 2.63 ± 0.062	5.988GluLeu: 5.988 ± 0.094	1.778GluMet: 1.778 ± 0.044	1.995GluAsn: 1.995 ± 0.045	3.033GluPro: 3.033 ± 0.063	2.495GluGln: 2.495 ± 0.051	5.516GluArg: 5.516 ± 0.084	2.74GluSer: 2.74 ± 0.057	3.644GluThr: 3.644 ± 0.062	4.148GluVal: 4.148 ± 0.063	0.941GluTrp: 0.941 ± 0.034	1.381GluTyr: 1.381 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	4.841PheAla: 4.841 ± 0.072	0.357PheCys: 0.357 ± 0.018	2.943PheAsp: 2.943 ± 0.064	2.51PheGlu: 2.51 ± 0.052	1.352PhePhe: 1.352 ± 0.042	3.608PheGly: 3.608 ± 0.065	0.68PheHis: 0.68 ± 0.025	1.53PheIle: 1.53 ± 0.041	0.93PheLys: 0.93 ± 0.028	3.109PheLeu: 3.109 ± 0.058	0.791PheMet: 0.791 ± 0.029	0.974PheAsn: 0.974 ± 0.039	1.514PhePro: 1.514 ± 0.039	0.924PheGln: 0.924 ± 0.031	2.2PheArg: 2.2 ± 0.05	2.123PheSer: 2.123 ± 0.048	1.955PheThr: 1.955 ± 0.05	2.674PheVal: 2.674 ± 0.054	0.525PheTrp: 0.525 ± 0.025	0.959PheTyr: 0.959 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	9.086GlyAla: 9.086 ± 0.102	0.82GlyCys: 0.82 ± 0.028	5.162GlyAsp: 5.162 ± 0.079	5.872GlyGlu: 5.872 ± 0.08	3.547GlyPhe: 3.547 ± 0.064	7.706GlyGly: 7.706 ± 0.134	1.847GlyHis: 1.847 ± 0.048	4.483GlyIle: 4.483 ± 0.072	3.546GlyLys: 3.546 ± 0.06	8.223GlyLeu: 8.223 ± 0.103	2.415GlyMet: 2.415 ± 0.052	2.344GlyAsn: 2.344 ± 0.06	3.467GlyPro: 3.467 ± 0.068	2.882GlyGln: 2.882 ± 0.054	5.91GlyArg: 5.91 ± 0.078	5.004GlySer: 5.004 ± 0.079	4.641GlyThr: 4.641 ± 0.082	5.706GlyVal: 5.706 ± 0.075	1.514GlyTrp: 1.514 ± 0.045	2.361GlyTyr: 2.361 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.251HisAla: 2.251 ± 0.056	0.263HisCys: 0.263 ± 0.018	1.262HisAsp: 1.262 ± 0.036	1.189HisGlu: 1.189 ± 0.037	0.869HisPhe: 0.869 ± 0.034	1.966HisGly: 1.966 ± 0.054	0.54HisHis: 0.54 ± 0.026	0.959HisIle: 0.959 ± 0.033	0.531HisLys: 0.531 ± 0.023	1.881HisLeu: 1.881 ± 0.045	0.452HisMet: 0.452 ± 0.023	0.496HisAsn: 0.496 ± 0.022	1.316HisPro: 1.316 ± 0.041	0.548HisGln: 0.548 ± 0.024	1.485HisArg: 1.485 ± 0.039	1.03HisSer: 1.03 ± 0.037	0.813HisThr: 0.813 ± 0.029	1.361HisVal: 1.361 ± 0.038	0.34HisTrp: 0.34 ± 0.018	0.567HisTyr: 0.567 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	7.761IleAla: 7.761 ± 0.1	0.522IleCys: 0.522 ± 0.024	4.034IleAsp: 4.034 ± 0.06	4.076IleGlu: 4.076 ± 0.069	1.635IlePhe: 1.635 ± 0.048	5.052IleGly: 5.052 ± 0.086	0.967IleHis: 0.967 ± 0.036	2.087IleIle: 2.087 ± 0.051	1.291IleLys: 1.291 ± 0.04	4.062IleLeu: 4.062 ± 0.065	0.975IleMet: 0.975 ± 0.035	1.258IleAsn: 1.258 ± 0.036	2.406IlePro: 2.406 ± 0.049	1.126IleGln: 1.126 ± 0.035	3.154IleArg: 3.154 ± 0.057	2.843IleSer: 2.843 ± 0.05	2.457IleThr: 2.457 ± 0.057	3.98IleVal: 3.98 ± 0.068	0.621IleTrp: 0.621 ± 0.028	1.013IleTyr: 1.013 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	4.273LysAla: 4.273 ± 0.071	0.196LysCys: 0.196 ± 0.015	1.847LysAsp: 1.847 ± 0.049	1.578LysGlu: 1.578 ± 0.043	1.01LysPhe: 1.01 ± 0.031	2.691LysGly: 2.691 ± 0.058	0.686LysHis: 0.686 ± 0.025	1.618LysIle: 1.618 ± 0.047	1.481LysLys: 1.481 ± 0.047	3.461LysLeu: 3.461 ± 0.065	0.817LysMet: 0.817 ± 0.027	0.853LysAsn: 0.853 ± 0.029	2.091LysPro: 2.091 ± 0.049	1.076LysGln: 1.076 ± 0.034	2.607LysArg: 2.607 ± 0.054	1.786LysSer: 1.786 ± 0.046	1.85LysThr: 1.85 ± 0.044	2.326LysVal: 2.326 ± 0.054	0.453LysTrp: 0.453 ± 0.021	0.691LysTyr: 0.691 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	13.637LeuAla: 13.637 ± 0.147	0.737LeuCys: 0.737 ± 0.027	6.437LeuAsp: 6.437 ± 0.094	5.811LeuGlu: 5.811 ± 0.08	3.521LeuPhe: 3.521 ± 0.067	8.039LeuGly: 8.039 ± 0.095	1.744LeuHis: 1.744 ± 0.043	4.431LeuIle: 4.431 ± 0.064	3.012LeuLys: 3.012 ± 0.067	8.907LeuLeu: 8.907 ± 0.129	2.05LeuMet: 2.05 ± 0.047	2.246LeuAsn: 2.246 ± 0.053	5.284LeuPro: 5.284 ± 0.072	2.389LeuGln: 2.389 ± 0.051	6.558LeuArg: 6.558 ± 0.094	5.853LeuSer: 5.853 ± 0.071	5.179LeuThr: 5.179 ± 0.072	7.219LeuVal: 7.219 ± 0.087	1.137LeuTrp: 1.137 ± 0.034	1.935LeuTyr: 1.935 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	3.257MetAla: 3.257 ± 0.06	0.181MetCys: 0.181 ± 0.013	1.275MetAsp: 1.275 ± 0.04	1.359MetGlu: 1.359 ± 0.037	0.652MetPhe: 0.652 ± 0.024	2.06MetGly: 2.06 ± 0.048	0.494MetHis: 0.494 ± 0.021	1.314MetIle: 1.314 ± 0.036	1.012MetLys: 1.012 ± 0.032	2.559MetLeu: 2.559 ± 0.046	0.681MetMet: 0.681 ± 0.028	0.78MetAsn: 0.78 ± 0.03	1.431MetPro: 1.431 ± 0.044	0.852MetGln: 0.852 ± 0.028	1.955MetArg: 1.955 ± 0.046	1.533MetSer: 1.533 ± 0.039	1.695MetThr: 1.695 ± 0.036	1.635MetVal: 1.635 ± 0.038	0.257MetTrp: 0.257 ± 0.016	0.304MetTyr: 0.304 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.246AsnAla: 3.246 ± 0.062	0.278AsnCys: 0.278 ± 0.017	1.494AsnAsp: 1.494 ± 0.043	1.427AsnGlu: 1.427 ± 0.04	1.008AsnPhe: 1.008 ± 0.034	2.458AsnGly: 2.458 ± 0.054	0.533AsnHis: 0.533 ± 0.022	1.352AsnIle: 1.352 ± 0.038	0.672AsnLys: 0.672 ± 0.026	2.493AsnLeu: 2.493 ± 0.056	0.608AsnMet: 0.608 ± 0.024	0.653AsnAsn: 0.653 ± 0.028	1.915AsnPro: 1.915 ± 0.044	0.805AsnGln: 0.805 ± 0.029	1.951AsnArg: 1.951 ± 0.051	1.383AsnSer: 1.383 ± 0.041	1.27AsnThr: 1.27 ± 0.039	1.77AsnVal: 1.77 ± 0.049	0.424AsnTrp: 0.424 ± 0.023	0.667AsnTyr: 0.667 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	5.711ProAla: 5.711 ± 0.085	0.379ProCys: 0.379 ± 0.018	3.848ProAsp: 3.848 ± 0.059	4.142ProGlu: 4.142 ± 0.067	1.915ProPhe: 1.915 ± 0.046	4.39ProGly: 4.39 ± 0.065	1.051ProHis: 1.051 ± 0.033	2.442ProIle: 2.442 ± 0.048	1.664ProLys: 1.664 ± 0.043	4.5ProLeu: 4.5 ± 0.067	1.213ProMet: 1.213 ± 0.034	1.311ProAsn: 1.311 ± 0.035	2.34ProPro: 2.34 ± 0.061	1.723ProGln: 1.723 ± 0.041	2.953ProArg: 2.953 ± 0.061	2.731ProSer: 2.731 ± 0.057	2.637ProThr: 2.637 ± 0.059	3.995ProVal: 3.995 ± 0.065	0.605ProTrp: 0.605 ± 0.025	1.08ProTyr: 1.08 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.87GlnAla: 3.87 ± 0.066	0.219GlnCys: 0.219 ± 0.013	1.536GlnAsp: 1.536 ± 0.043	1.656GlnGlu: 1.656 ± 0.041	1.16GlnPhe: 1.16 ± 0.037	2.443GlnGly: 2.443 ± 0.051	0.609GlnHis: 0.609 ± 0.024	1.759GlnIle: 1.759 ± 0.047	0.939GlnLys: 0.939 ± 0.028	3.108GlnLeu: 3.108 ± 0.062	0.857GlnMet: 0.857 ± 0.025	0.797GlnAsn: 0.797 ± 0.034	1.732GlnPro: 1.732 ± 0.046	1.144GlnGln: 1.144 ± 0.039	2.497GlnArg: 2.497 ± 0.052	1.758GlnSer: 1.758 ± 0.046	1.634GlnThr: 1.634 ± 0.045	2.274GlnVal: 2.274 ± 0.047	0.424GlnTrp: 0.424 ± 0.022	0.654GlnTyr: 0.654 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.948ArgAla: 7.948 ± 0.101	0.524ArgCys: 0.524 ± 0.025	4.385ArgAsp: 4.385 ± 0.063	5.034ArgGlu: 5.034 ± 0.077	3.006ArgPhe: 3.006 ± 0.057	5.087ArgGly: 5.087 ± 0.082	1.596ArgHis: 1.596 ± 0.039	4.148ArgIle: 4.148 ± 0.068	2.537ArgLys: 2.537 ± 0.056	7.595ArgLeu: 7.595 ± 0.103	2.051ArgMet: 2.051 ± 0.044	1.943ArgAsn: 1.943 ± 0.039	3.258ArgPro: 3.258 ± 0.058	2.517ArgGln: 2.517 ± 0.055	5.302ArgArg: 5.302 ± 0.092	3.674ArgSer: 3.674 ± 0.065	3.295ArgThr: 3.295 ± 0.051	4.57ArgVal: 4.57 ± 0.066	1.147ArgTrp: 1.147 ± 0.032	1.81ArgTyr: 1.81 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	6.156SerAla: 6.156 ± 0.095	0.479SerCys: 0.479 ± 0.026	3.444SerAsp: 3.444 ± 0.072	3.348SerGlu: 3.348 ± 0.06	2.166SerPhe: 2.166 ± 0.056	5.438SerGly: 5.438 ± 0.081	1.035SerHis: 1.035 ± 0.031	2.749SerIle: 2.749 ± 0.057	1.634SerLys: 1.634 ± 0.044	5.148SerLeu: 5.148 ± 0.082	1.346SerMet: 1.346 ± 0.038	1.498SerAsn: 1.498 ± 0.043	2.849SerPro: 2.849 ± 0.055	1.665SerGln: 1.665 ± 0.043	3.72SerArg: 3.72 ± 0.062	2.938SerSer: 2.938 ± 0.068	2.726SerThr: 2.726 ± 0.067	3.611SerVal: 3.611 ± 0.062	0.765SerTrp: 0.765 ± 0.029	1.446SerTyr: 1.446 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	5.858ThrAla: 5.858 ± 0.082	0.424ThrCys: 0.424 ± 0.022	3.039ThrAsp: 3.039 ± 0.063	2.822ThrGlu: 2.822 ± 0.058	1.877ThrPhe: 1.877 ± 0.046	5.084ThrGly: 5.084 ± 0.084	1.011ThrHis: 1.011 ± 0.031	3.025ThrIle: 3.025 ± 0.056	1.568ThrLys: 1.568 ± 0.043	5.296ThrLeu: 5.296 ± 0.075	1.226ThrMet: 1.226 ± 0.032	1.323ThrAsn: 1.323 ± 0.046	3.07ThrPro: 3.07 ± 0.058	1.46ThrGln: 1.46 ± 0.036	3.411ThrArg: 3.411 ± 0.065	2.854ThrSer: 2.854 ± 0.069	2.536ThrThr: 2.536 ± 0.053	3.843ThrVal: 3.843 ± 0.08	0.611ThrTrp: 0.611 ± 0.026	1.261ThrTyr: 1.261 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	8.325ValAla: 8.325 ± 0.104	0.545ValCys: 0.545 ± 0.022	4.43ValAsp: 4.43 ± 0.063	5.134ValGlu: 5.134 ± 0.069	2.322ValPhe: 2.322 ± 0.059	5.368ValGly: 5.368 ± 0.072	1.266ValHis: 1.266 ± 0.031	3.667ValIle: 3.667 ± 0.064	2.028ValLys: 2.028 ± 0.055	6.447ValLeu: 6.447 ± 0.09	1.631ValMet: 1.631 ± 0.04	1.857ValAsn: 1.857 ± 0.051	3.667ValPro: 3.667 ± 0.055	1.894ValGln: 1.894 ± 0.045	4.613ValArg: 4.613 ± 0.068	3.993ValSer: 3.993 ± 0.076	4.21ValThr: 4.21 ± 0.073	5.016ValVal: 5.016 ± 0.078	0.846ValTrp: 0.846 ± 0.028	1.288ValTyr: 1.288 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.308TrpAla: 1.308 ± 0.035	0.127TrpCys: 0.127 ± 0.012	0.777TrpAsp: 0.777 ± 0.029	0.732TrpGlu: 0.732 ± 0.027	0.557TrpPhe: 0.557 ± 0.022	0.927TrpGly: 0.927 ± 0.035	0.373TrpHis: 0.373 ± 0.018	0.732TrpIle: 0.732 ± 0.028	0.525TrpLys: 0.525 ± 0.024	1.647TrpLeu: 1.647 ± 0.044	0.361TrpMet: 0.361 ± 0.019	0.446TrpAsn: 0.446 ± 0.024	0.634TrpPro: 0.634 ± 0.026	0.613TrpGln: 0.613 ± 0.029	1.238TrpArg: 1.238 ± 0.037	0.894TrpSer: 0.894 ± 0.034	0.773TrpThr: 0.773 ± 0.031	0.813TrpVal: 0.813 ± 0.03	0.258TrpTrp: 0.258 ± 0.02	0.275TrpTyr: 0.275 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.495TyrAla: 2.495 ± 0.05	0.248TyrCys: 0.248 ± 0.017	1.734TyrAsp: 1.734 ± 0.046	1.405TyrGlu: 1.405 ± 0.043	0.854TyrPhe: 0.854 ± 0.032	2.121TyrGly: 2.121 ± 0.047	0.513TyrHis: 0.513 ± 0.023	0.951TyrIle: 0.951 ± 0.034	0.564TyrLys: 0.564 ± 0.025	2.133TyrLeu: 2.133 ± 0.049	0.412TyrMet: 0.412 ± 0.022	0.612TyrAsn: 0.612 ± 0.029	1.054TyrPro: 1.054 ± 0.033	0.679TyrGln: 0.679 ± 0.023	1.948TyrArg: 1.948 ± 0.052	1.333TyrSer: 1.333 ± 0.041	1.133TyrThr: 1.133 ± 0.036	1.512TyrVal: 1.512 ± 0.041	0.362TyrTrp: 0.362 ± 0.018	0.614TyrTyr: 0.614 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3245 proteins (978490 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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