Ngaingan hapavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Hapavirus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3GGL5|D3GGL5_9RHAB Matrix protein OS=Ngaingan hapavirus OX=1972623 GN=M PE=4 SV=1
MM1 pKa = 7.16EE2 pKa = 4.51TLKK5 pKa = 10.08TFAVMAINTNEE16 pKa = 3.87SDD18 pKa = 4.82DD19 pKa = 3.79VDD21 pKa = 3.71GLIRR25 pKa = 11.84MMLRR29 pKa = 11.84KK30 pKa = 9.49NRR32 pKa = 11.84YY33 pKa = 7.9KK34 pKa = 10.44VKK36 pKa = 9.17GWNRR40 pKa = 11.84ISKK43 pKa = 10.28LVGEE47 pKa = 4.69WCLQSLEE54 pKa = 4.37DD55 pKa = 3.79VGDD58 pKa = 3.78GNMVGTFSASIDD70 pKa = 3.71HH71 pKa = 7.11PISIQFLEE79 pKa = 4.35HH80 pKa = 6.57KK81 pKa = 10.12RR82 pKa = 11.84LEE84 pKa = 4.01EE85 pKa = 4.0TFYY88 pKa = 11.14SIYY91 pKa = 10.1DD92 pKa = 3.44TGTCRR97 pKa = 11.84IEE99 pKa = 3.99LFYY102 pKa = 10.94KK103 pKa = 10.19IIRR106 pKa = 11.84WLDD109 pKa = 3.47EE110 pKa = 4.19SPEE113 pKa = 3.89SHH115 pKa = 6.3QRR117 pKa = 11.84IEE119 pKa = 4.34NFWVDD124 pKa = 2.8NHH126 pKa = 6.53EE127 pKa = 4.14YY128 pKa = 10.84HH129 pKa = 6.85NVSTLQTAEE138 pKa = 4.28MLNDD142 pKa = 4.64LIIGG146 pKa = 3.96

Molecular weight: 17.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3GGL3|D3GGL3_9RHAB Isoform of D3GGL2 Uncharacterized protein U1/U2 OS=Ngaingan hapavirus OX=1972623 GN=U1/U2 PE=4 SV=1
MM1 pKa = 6.92SCKK4 pKa = 8.93MHH6 pKa = 6.63MEE8 pKa = 4.84HH9 pKa = 6.64IWFAKK14 pKa = 7.06TTIKK18 pKa = 11.13DD19 pKa = 3.8MIQKK23 pKa = 9.97NLTRR27 pKa = 11.84QIQSHH32 pKa = 5.37QLPHH36 pKa = 6.46PKK38 pKa = 9.77PRR40 pKa = 11.84VQHH43 pKa = 6.41LGNGLRR49 pKa = 11.84CWKK52 pKa = 9.19MALRR56 pKa = 11.84SSS58 pKa = 3.68

Molecular weight: 6.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

4

15

5254

58

2105

350.3

40.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.864 ± 0.724

2.037 ± 0.186

5.577 ± 0.336

5.938 ± 0.304

4.34 ± 0.405

5.52 ± 0.38

2.969 ± 0.195

8.375 ± 0.668

7.156 ± 0.335

9.364 ± 0.514

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.259

5.101 ± 0.23

4.397 ± 0.411

3.255 ± 0.221

3.959 ± 0.281

8.413 ± 0.332

5.824 ± 0.54

5.139 ± 0.429

1.96 ± 0.161

4.359 ± 0.447
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski