Halioglobus sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Halieaceae; Halioglobus

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L7DX27|A0A3L7DX27_9GAMM Dihydroxy-acid dehydratase OS=Halioglobus sediminis OX=2283635 GN=ilvD PE=3 SV=1
MM1 pKa = 7.43AVALTLLLAIAACGGGGGGGGGFKK25 pKa = 10.82GDD27 pKa = 3.72GGSGGDD33 pKa = 4.36ADD35 pKa = 4.01NDD37 pKa = 4.15TYY39 pKa = 10.99FIQLALRR46 pKa = 11.84DD47 pKa = 4.21SEE49 pKa = 4.97GEE51 pKa = 3.76PSSIVTSNSPGTLQVLVTEE70 pKa = 5.14KK71 pKa = 10.49NAKK74 pKa = 9.31GDD76 pKa = 3.86PVPDD80 pKa = 4.08VIVTASTDD88 pKa = 3.36SGLLDD93 pKa = 4.01PASGNALTNAEE104 pKa = 4.17GAVTFTVRR112 pKa = 11.84AGQDD116 pKa = 2.77RR117 pKa = 11.84GAGTIVVSVEE127 pKa = 3.91DD128 pKa = 3.62PAGVVIEE135 pKa = 4.49EE136 pKa = 4.49SVNFQIGIGDD146 pKa = 3.79LRR148 pKa = 11.84LGHH151 pKa = 6.47LQGSSFFDD159 pKa = 3.49AEE161 pKa = 4.06IGITPEE167 pKa = 3.83GPIAARR173 pKa = 11.84GEE175 pKa = 4.68AILSLAIVDD184 pKa = 4.2PKK186 pKa = 10.97GLPIGTAEE194 pKa = 4.15SIKK197 pKa = 10.68INSLCLDD204 pKa = 3.63IGDD207 pKa = 4.83ASLSPEE213 pKa = 3.9NPIPVVDD220 pKa = 4.02GRR222 pKa = 11.84VEE224 pKa = 4.04VTYY227 pKa = 9.25TAAGCQGMDD236 pKa = 3.27EE237 pKa = 4.44LTAEE241 pKa = 4.47VIGVGAQAFGTIEE254 pKa = 3.91ISDD257 pKa = 4.28TIANGLTFVSAEE269 pKa = 3.88PNLIVLRR276 pKa = 11.84GTGGGPTRR284 pKa = 11.84QEE286 pKa = 3.7KK287 pKa = 11.01SEE289 pKa = 4.1VTFQAVDD296 pKa = 3.42ANAQPLEE303 pKa = 4.22GVDD306 pKa = 3.65VVFSLTTDD314 pKa = 3.01VGGLSFSPQSATTGADD330 pKa = 3.32GTASTVVSSGDD341 pKa = 3.16VATVVRR347 pKa = 11.84VVATAEE353 pKa = 4.0ADD355 pKa = 3.66DD356 pKa = 4.78LGEE359 pKa = 4.07VSAVSDD365 pKa = 3.83VLTVSTGLPDD375 pKa = 3.37QNSISLSVDD384 pKa = 2.87GNFVVEE390 pKa = 4.51EE391 pKa = 4.41GMTKK395 pKa = 10.58DD396 pKa = 3.08GVTRR400 pKa = 11.84QLTVRR405 pKa = 11.84MADD408 pKa = 3.12KK409 pKa = 10.75FNNPVPDD416 pKa = 3.34GTAAVFTTEE425 pKa = 3.69YY426 pKa = 11.08GAIQSSCEE434 pKa = 4.12TVGGQCSVTWNSQAPRR450 pKa = 11.84VPTLNEE456 pKa = 3.77NQDD459 pKa = 3.76LVVTIFDD466 pKa = 4.45PGYY469 pKa = 10.57DD470 pKa = 3.81CPSHH474 pKa = 6.5NGSSGPCPDD483 pKa = 4.36DD484 pKa = 4.67LGMIRR489 pKa = 11.84GGRR492 pKa = 11.84STILVTAIGEE502 pKa = 4.1EE503 pKa = 4.24SFIDD507 pKa = 3.76SNANGIYY514 pKa = 10.28DD515 pKa = 3.56EE516 pKa = 4.83GEE518 pKa = 3.76RR519 pKa = 11.84FANQSEE525 pKa = 4.24AFLDD529 pKa = 3.56INEE532 pKa = 4.4NGFYY536 pKa = 10.6DD537 pKa = 4.82QSTTACNNNPDD548 pKa = 4.03TLTCKK553 pKa = 10.4AGSEE557 pKa = 4.43EE558 pKa = 4.27IFTDD562 pKa = 4.83FNSNGVFDD570 pKa = 5.81ANGDD574 pKa = 3.97DD575 pKa = 4.33EE576 pKa = 6.05LNGYY580 pKa = 8.71PDD582 pKa = 3.91EE583 pKa = 4.83GVQALYY589 pKa = 11.03NGLLCPKK596 pKa = 9.49EE597 pKa = 3.95GDD599 pKa = 3.3GVYY602 pKa = 10.44CSRR605 pKa = 11.84EE606 pKa = 3.89LLNVNDD612 pKa = 4.26SLVLILSTDD621 pKa = 3.99PNWDD625 pKa = 2.89IALYY629 pKa = 10.53RR630 pKa = 11.84GVTPANSTTYY640 pKa = 10.98NGGTYY645 pKa = 7.97TAYY648 pKa = 9.91ISDD651 pKa = 4.55YY652 pKa = 10.58YY653 pKa = 10.8NSKK656 pKa = 8.5PTGGSTVSIEE666 pKa = 3.85ASGDD670 pKa = 3.61CEE672 pKa = 4.05ITGKK676 pKa = 10.69EE677 pKa = 4.08SFEE680 pKa = 4.42VPNTTAPGAFAVSFSQGGVGEE701 pKa = 4.25EE702 pKa = 3.97AGEE705 pKa = 4.3VTVTLSPNGGGPDD718 pKa = 3.78YY719 pKa = 11.23SEE721 pKa = 4.9SWPCTPEE728 pKa = 4.04PEE730 pKa = 4.26PVEE733 pKa = 4.89PEE735 pKa = 3.87PCDD738 pKa = 3.75PNVEE742 pKa = 4.16LCAGGG747 pKa = 3.36

Molecular weight: 76.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L7DXU2|A0A3L7DXU2_9GAMM GTP 3' 8-cyclase OS=Halioglobus sediminis OX=2283635 GN=moaA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.87RR14 pKa = 11.84THH16 pKa = 5.99GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.47NGRR28 pKa = 11.84AVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3770

0

3770

1298764

29

4582

344.5

37.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.081 ± 0.052

1.06 ± 0.015

5.944 ± 0.038

6.322 ± 0.036

3.569 ± 0.025

8.156 ± 0.035

2.149 ± 0.017

4.904 ± 0.028

2.966 ± 0.03

10.8 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.02

3.017 ± 0.023

4.776 ± 0.026

4.144 ± 0.024

6.691 ± 0.036

5.897 ± 0.024

4.786 ± 0.027

7.078 ± 0.032

1.4 ± 0.019

2.822 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski