Imperata yellow mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5WYM8|B5WYM8_9VIRU Capsid protein OS=Imperata yellow mottle virus OX=524023 PE=3 SV=1
MM1 pKa = 7.92PLTAVGRR8 pKa = 11.84SDD10 pKa = 3.13CRR12 pKa = 11.84FRR14 pKa = 11.84EE15 pKa = 4.93GTQKK19 pKa = 10.75PPGPRR24 pKa = 11.84VSAAVRR30 pKa = 11.84VFPEE34 pKa = 4.01LADD37 pKa = 3.87YY38 pKa = 10.15AWPARR43 pKa = 11.84GSEE46 pKa = 4.38AEE48 pKa = 4.19LEE50 pKa = 4.32SLRR53 pKa = 11.84VQASRR58 pKa = 11.84FKK60 pKa = 10.32RR61 pKa = 11.84TLPPPSLQEE70 pKa = 3.99AVNALQGRR78 pKa = 11.84YY79 pKa = 9.15PNSQVRR85 pKa = 11.84KK86 pKa = 9.6CFRR89 pKa = 11.84QEE91 pKa = 3.36PWDD94 pKa = 3.95FQALCEE100 pKa = 4.01EE101 pKa = 4.55VQRR104 pKa = 11.84ICQTGEE110 pKa = 3.87LNGTSSPGVPLANIEE125 pKa = 4.19NQNAAVLNLAPEE137 pKa = 4.53LVHH140 pKa = 6.71LAVAEE145 pKa = 4.06RR146 pKa = 11.84LVALAAVDD154 pKa = 3.88PRR156 pKa = 11.84QHH158 pKa = 5.41NWTPKK163 pKa = 9.52EE164 pKa = 3.76LVQRR168 pKa = 11.84GLVDD172 pKa = 3.62PVRR175 pKa = 11.84LFVKK179 pKa = 10.12QEE181 pKa = 3.57PHH183 pKa = 4.42TMRR186 pKa = 11.84KK187 pKa = 8.0IRR189 pKa = 11.84EE190 pKa = 3.7RR191 pKa = 11.84RR192 pKa = 11.84FRR194 pKa = 11.84LISSVSLVDD203 pKa = 3.53QLVEE207 pKa = 4.17RR208 pKa = 11.84MLFGPQNATEE218 pKa = 3.6ISMWHH223 pKa = 6.65LCPSKK228 pKa = 10.38PGMGMSTPSQVEE240 pKa = 4.18MLWKK244 pKa = 10.5DD245 pKa = 3.58VAHH248 pKa = 6.47KK249 pKa = 10.46HH250 pKa = 5.59SLHH253 pKa = 5.74QAAEE257 pKa = 4.0ADD259 pKa = 3.05ISAFDD264 pKa = 3.67WSVQDD269 pKa = 3.38WEE271 pKa = 4.52LWADD275 pKa = 3.73LAIRR279 pKa = 11.84LQLGSFPDD287 pKa = 3.67LMRR290 pKa = 11.84RR291 pKa = 11.84AAISRR296 pKa = 11.84FYY298 pKa = 11.3CFMNSVFQLSDD309 pKa = 3.11GTLIEE314 pKa = 4.14QNLPGLMKK322 pKa = 10.49SGSYY326 pKa = 8.59CTSSTNSRR334 pKa = 11.84IRR336 pKa = 11.84CLMAEE341 pKa = 4.85LIGSPWCIAMGDD353 pKa = 3.8DD354 pKa = 4.68SVEE357 pKa = 3.99GWVEE361 pKa = 4.15GAQEE365 pKa = 3.91KK366 pKa = 10.09YY367 pKa = 10.39AALGHH372 pKa = 5.17TCKK375 pKa = 10.4EE376 pKa = 4.28YY377 pKa = 10.75VACPSKK383 pKa = 10.5QGRR386 pKa = 11.84LGKK389 pKa = 10.11EE390 pKa = 3.29LLSFNFCSHH399 pKa = 6.38EE400 pKa = 4.01FTNPGLPRR408 pKa = 11.84AEE410 pKa = 4.04LLTWAKK416 pKa = 10.1CLYY419 pKa = 10.38RR420 pKa = 11.84FLSSNRR426 pKa = 11.84EE427 pKa = 4.01TVDD430 pKa = 3.96DD431 pKa = 4.67LWVEE435 pKa = 4.52LNTSRR440 pKa = 11.84QWGRR444 pKa = 11.84IQKK447 pKa = 8.37YY448 pKa = 8.9LAGIGEE454 pKa = 4.27VSQLNSADD462 pKa = 3.77GEE464 pKa = 4.35IKK466 pKa = 10.72AEE468 pKa = 4.33EE469 pKa = 4.13IQEE472 pKa = 4.18DD473 pKa = 3.81AAGCGGQEE481 pKa = 3.9PAASAFEE488 pKa = 4.04EE489 pKa = 4.23ARR491 pKa = 11.84EE492 pKa = 3.94IWIEE496 pKa = 3.86PTPAQQWDD504 pKa = 4.2NNIGPHH510 pKa = 5.68GYY512 pKa = 9.2EE513 pKa = 3.73WDD515 pKa = 3.66WPFSEE520 pKa = 5.26SWSPWGRR527 pKa = 11.84DD528 pKa = 3.16HH529 pKa = 7.69SS530 pKa = 4.09

Molecular weight: 59.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5WYM8|B5WYM8_9VIRU Capsid protein OS=Imperata yellow mottle virus OX=524023 PE=3 SV=1
MM1 pKa = 7.31GRR3 pKa = 11.84SKK5 pKa = 10.63QKK7 pKa = 9.9KK8 pKa = 7.56SKK10 pKa = 10.45RR11 pKa = 11.84MPQAAAVKK19 pKa = 8.83NQQPAPSRR27 pKa = 11.84RR28 pKa = 11.84QGRR31 pKa = 11.84SGSSQRR37 pKa = 11.84QPSNGITTSAPMATSGIGPSRR58 pKa = 11.84SLGPLGGAITVEE70 pKa = 4.42EE71 pKa = 4.56VEE73 pKa = 4.31HH74 pKa = 6.83LGAFTRR80 pKa = 11.84GTTAAGQTRR89 pKa = 11.84AYY91 pKa = 10.8LCMPSSLIRR100 pKa = 11.84LSNIARR106 pKa = 11.84CFARR110 pKa = 11.84WRR112 pKa = 11.84PLQWSVFYY120 pKa = 10.85VPEE123 pKa = 4.58VGTQTNGAIQMAYY136 pKa = 10.21LYY138 pKa = 10.09DD139 pKa = 4.12YY140 pKa = 11.13GDD142 pKa = 4.2SLPTTTGQISACSGFLTAAVWCGAAGAQLLSGKK175 pKa = 10.13APRR178 pKa = 11.84SQNLVIAHH186 pKa = 5.7MDD188 pKa = 3.43CRR190 pKa = 11.84RR191 pKa = 11.84SEE193 pKa = 3.75WMRR196 pKa = 11.84VVDD199 pKa = 5.32GLTDD203 pKa = 3.41TDD205 pKa = 4.03PAHH208 pKa = 6.39VVNTYY213 pKa = 10.26LPARR217 pKa = 11.84AVVRR221 pKa = 11.84SSLLPSTEE229 pKa = 3.93DD230 pKa = 3.2TPGQLYY236 pKa = 9.44VRR238 pKa = 11.84YY239 pKa = 9.99RR240 pKa = 11.84IMLRR244 pKa = 11.84DD245 pKa = 3.52AVAPGLNDD253 pKa = 3.53ASSSAPSVDD262 pKa = 3.76QEE264 pKa = 4.43RR265 pKa = 11.84KK266 pKa = 9.36GNAAVVSS273 pKa = 4.07

Molecular weight: 29.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1614

213

598

403.5

44.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.993 ± 1.142

2.23 ± 0.357

4.337 ± 0.319

6.134 ± 1.031

2.85 ± 0.424

7.249 ± 0.642

2.292 ± 0.406

3.408 ± 0.27

3.47 ± 0.583

8.488 ± 0.775

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.107 ± 0.316

3.346 ± 0.153

6.629 ± 0.241

4.833 ± 0.523

7.621 ± 0.794

10.223 ± 0.926

5.328 ± 0.765

6.629 ± 0.281

2.292 ± 0.485

2.54 ± 0.431
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski