Amino acid dipepetide frequency for Staphylococcus phage SpaA1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.613AlaAla: 5.613 ± 1.53
0.16AlaCys: 0.16 ± 0.103
3.688AlaAsp: 3.688 ± 0.539
5.532AlaGlu: 5.532 ± 0.593
2.165AlaPhe: 2.165 ± 0.376
3.608AlaGly: 3.608 ± 0.577
0.722AlaHis: 0.722 ± 0.229
4.41AlaIle: 4.41 ± 0.606
5.212AlaLys: 5.212 ± 0.524
5.212AlaLeu: 5.212 ± 0.79
1.924AlaMet: 1.924 ± 0.304
3.287AlaAsn: 3.287 ± 0.625
1.523AlaPro: 1.523 ± 0.259
2.245AlaGln: 2.245 ± 0.533
3.768AlaArg: 3.768 ± 0.496
3.287AlaSer: 3.287 ± 0.481
3.448AlaThr: 3.448 ± 0.503
3.768AlaVal: 3.768 ± 0.628
1.042AlaTrp: 1.042 ± 0.226
1.604AlaTyr: 1.604 ± 0.41
0.0AlaXaa: 0.0 ± 0.0
Cys
0.401CysAla: 0.401 ± 0.201
0.16CysCys: 0.16 ± 0.114
0.882CysAsp: 0.882 ± 0.36
0.722CysGlu: 0.722 ± 0.228
0.561CysPhe: 0.561 ± 0.229
0.481CysGly: 0.481 ± 0.223
0.321CysHis: 0.321 ± 0.175
0.241CysIle: 0.241 ± 0.126
0.401CysLys: 0.401 ± 0.188
0.321CysLeu: 0.321 ± 0.199
0.561CysMet: 0.561 ± 0.233
0.481CysAsn: 0.481 ± 0.259
0.16CysPro: 0.16 ± 0.109
0.241CysGln: 0.241 ± 0.12
0.802CysArg: 0.802 ± 0.251
0.401CysSer: 0.401 ± 0.184
0.481CysThr: 0.481 ± 0.172
0.321CysVal: 0.321 ± 0.158
0.0CysTrp: 0.0 ± 0.0
0.241CysTyr: 0.241 ± 0.162
0.0CysXaa: 0.0 ± 0.0
Asp
3.207AspAla: 3.207 ± 0.57
0.401AspCys: 0.401 ± 0.179
3.368AspAsp: 3.368 ± 0.548
5.372AspGlu: 5.372 ± 0.751
2.325AspPhe: 2.325 ± 0.413
5.292AspGly: 5.292 ± 0.727
1.123AspHis: 1.123 ± 0.296
4.65AspIle: 4.65 ± 0.612
4.25AspLys: 4.25 ± 0.567
4.33AspLeu: 4.33 ± 0.608
2.486AspMet: 2.486 ± 0.427
2.325AspAsn: 2.325 ± 0.4
1.844AspPro: 1.844 ± 0.363
1.604AspGln: 1.604 ± 0.441
2.245AspArg: 2.245 ± 0.368
2.245AspSer: 2.245 ± 0.368
3.287AspThr: 3.287 ± 0.484
3.768AspVal: 3.768 ± 0.522
1.123AspTrp: 1.123 ± 0.312
2.165AspTyr: 2.165 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
6.094GluAla: 6.094 ± 0.719
0.641GluCys: 0.641 ± 0.23
3.849GluAsp: 3.849 ± 0.462
7.216GluGlu: 7.216 ± 0.963
3.768GluPhe: 3.768 ± 0.577
4.33GluGly: 4.33 ± 0.455
1.844GluHis: 1.844 ± 0.446
6.495GluIle: 6.495 ± 0.782
8.258GluLys: 8.258 ± 0.861
7.136GluLeu: 7.136 ± 0.649
2.806GluMet: 2.806 ± 0.732
4.009GluAsn: 4.009 ± 0.494
1.924GluPro: 1.924 ± 0.457
3.608GluGln: 3.608 ± 0.657
4.169GluArg: 4.169 ± 0.582
4.57GluSer: 4.57 ± 0.634
4.25GluThr: 4.25 ± 0.561
5.051GluVal: 5.051 ± 0.584
1.684GluTrp: 1.684 ± 0.289
3.047GluTyr: 3.047 ± 0.637
0.0GluXaa: 0.0 ± 0.0
Phe
2.004PheAla: 2.004 ± 0.385
0.401PheCys: 0.401 ± 0.175
2.325PheAsp: 2.325 ± 0.409
3.047PheGlu: 3.047 ± 0.497
1.764PhePhe: 1.764 ± 0.47
4.089PheGly: 4.089 ± 0.502
0.561PheHis: 0.561 ± 0.208
3.929PheIle: 3.929 ± 0.531
4.25PheLys: 4.25 ± 0.55
3.047PheLeu: 3.047 ± 0.577
1.042PheMet: 1.042 ± 0.275
2.245PheAsn: 2.245 ± 0.418
0.882PhePro: 0.882 ± 0.309
1.523PheGln: 1.523 ± 0.307
1.844PheArg: 1.844 ± 0.369
2.405PheSer: 2.405 ± 0.447
2.486PheThr: 2.486 ± 0.457
2.325PheVal: 2.325 ± 0.376
0.561PheTrp: 0.561 ± 0.198
1.523PheTyr: 1.523 ± 0.349
0.0PheXaa: 0.0 ± 0.0
Gly
3.929GlyAla: 3.929 ± 0.905
0.08GlyCys: 0.08 ± 0.071
3.207GlyAsp: 3.207 ± 0.509
4.25GlyGlu: 4.25 ± 0.562
2.004GlyPhe: 2.004 ± 0.462
2.566GlyGly: 2.566 ± 0.418
0.722GlyHis: 0.722 ± 0.254
4.65GlyIle: 4.65 ± 0.776
6.495GlyLys: 6.495 ± 0.759
5.452GlyLeu: 5.452 ± 0.626
2.165GlyMet: 2.165 ± 0.293
2.405GlyAsn: 2.405 ± 0.36
0.722GlyPro: 0.722 ± 0.247
1.604GlyGln: 1.604 ± 0.372
2.806GlyArg: 2.806 ± 0.44
3.528GlySer: 3.528 ± 0.495
3.287GlyThr: 3.287 ± 0.469
4.65GlyVal: 4.65 ± 0.872
1.283GlyTrp: 1.283 ± 0.362
3.448GlyTyr: 3.448 ± 0.567
0.0GlyXaa: 0.0 ± 0.0
His
0.882HisAla: 0.882 ± 0.257
0.401HisCys: 0.401 ± 0.175
0.722HisAsp: 0.722 ± 0.201
1.042HisGlu: 1.042 ± 0.253
0.802HisPhe: 0.802 ± 0.272
1.042HisGly: 1.042 ± 0.292
0.241HisHis: 0.241 ± 0.133
0.722HisIle: 0.722 ± 0.17
1.924HisLys: 1.924 ± 0.446
1.764HisLeu: 1.764 ± 0.401
0.321HisMet: 0.321 ± 0.15
0.962HisAsn: 0.962 ± 0.299
0.0HisPro: 0.0 ± 0.0
0.882HisGln: 0.882 ± 0.276
0.481HisArg: 0.481 ± 0.225
1.523HisSer: 1.523 ± 0.384
1.443HisThr: 1.443 ± 0.301
1.443HisVal: 1.443 ± 0.232
0.16HisTrp: 0.16 ± 0.089
0.641HisTyr: 0.641 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
5.693IleAla: 5.693 ± 0.765
0.802IleCys: 0.802 ± 0.221
4.33IleAsp: 4.33 ± 0.582
6.254IleGlu: 6.254 ± 0.615
2.646IlePhe: 2.646 ± 0.432
3.368IleGly: 3.368 ± 0.529
1.283IleHis: 1.283 ± 0.357
5.212IleIle: 5.212 ± 0.63
6.976IleLys: 6.976 ± 0.766
4.57IleLeu: 4.57 ± 0.554
1.764IleMet: 1.764 ± 0.39
5.613IleAsn: 5.613 ± 0.854
2.486IlePro: 2.486 ± 0.43
3.368IleGln: 3.368 ± 0.569
2.886IleArg: 2.886 ± 0.427
4.49IleSer: 4.49 ± 0.529
4.25IleThr: 4.25 ± 0.551
4.169IleVal: 4.169 ± 0.656
1.042IleTrp: 1.042 ± 0.233
1.924IleTyr: 1.924 ± 0.347
0.0IleXaa: 0.0 ± 0.0
Lys
5.452LysAla: 5.452 ± 0.553
0.722LysCys: 0.722 ± 0.277
5.212LysAsp: 5.212 ± 0.876
10.423LysGlu: 10.423 ± 0.991
2.806LysPhe: 2.806 ± 0.401
4.009LysGly: 4.009 ± 0.74
1.604LysHis: 1.604 ± 0.294
6.334LysIle: 6.334 ± 0.646
8.419LysLys: 8.419 ± 1.005
6.976LysLeu: 6.976 ± 0.691
2.967LysMet: 2.967 ± 0.504
4.33LysAsn: 4.33 ± 0.666
3.047LysPro: 3.047 ± 0.417
5.532LysGln: 5.532 ± 0.658
4.971LysArg: 4.971 ± 0.698
4.891LysSer: 4.891 ± 0.691
5.532LysThr: 5.532 ± 0.638
6.735LysVal: 6.735 ± 0.585
1.042LysTrp: 1.042 ± 0.312
3.207LysTyr: 3.207 ± 0.533
0.0LysXaa: 0.0 ± 0.0
Leu
4.891LeuAla: 4.891 ± 0.7
0.962LeuCys: 0.962 ± 0.33
5.212LeuAsp: 5.212 ± 0.565
6.655LeuGlu: 6.655 ± 0.552
2.566LeuPhe: 2.566 ± 0.433
4.971LeuGly: 4.971 ± 0.715
1.764LeuHis: 1.764 ± 0.469
5.613LeuIle: 5.613 ± 0.664
8.018LeuLys: 8.018 ± 0.806
5.051LeuLeu: 5.051 ± 0.802
1.443LeuMet: 1.443 ± 0.34
4.65LeuAsn: 4.65 ± 0.612
2.566LeuPro: 2.566 ± 0.361
3.608LeuGln: 3.608 ± 0.459
3.047LeuArg: 3.047 ± 0.339
5.773LeuSer: 5.773 ± 0.703
3.768LeuThr: 3.768 ± 0.474
4.891LeuVal: 4.891 ± 0.66
0.641LeuTrp: 0.641 ± 0.252
2.646LeuTyr: 2.646 ± 0.449
0.0LeuXaa: 0.0 ± 0.0
Met
1.363MetAla: 1.363 ± 0.386
0.16MetCys: 0.16 ± 0.112
1.604MetAsp: 1.604 ± 0.443
2.245MetGlu: 2.245 ± 0.396
2.004MetPhe: 2.004 ± 0.592
1.604MetGly: 1.604 ± 0.323
0.321MetHis: 0.321 ± 0.197
1.924MetIle: 1.924 ± 0.42
4.57MetLys: 4.57 ± 0.655
2.325MetLeu: 2.325 ± 0.51
0.641MetMet: 0.641 ± 0.202
2.245MetAsn: 2.245 ± 0.459
0.802MetPro: 0.802 ± 0.296
1.042MetGln: 1.042 ± 0.285
1.203MetArg: 1.203 ± 0.325
1.604MetSer: 1.604 ± 0.33
2.004MetThr: 2.004 ± 0.384
1.363MetVal: 1.363 ± 0.303
0.321MetTrp: 0.321 ± 0.156
0.722MetTyr: 0.722 ± 0.318
0.0MetXaa: 0.0 ± 0.0
Asn
3.287AsnAla: 3.287 ± 0.653
0.321AsnCys: 0.321 ± 0.217
2.886AsnAsp: 2.886 ± 0.461
5.051AsnGlu: 5.051 ± 0.582
1.764AsnPhe: 1.764 ± 0.449
4.33AsnGly: 4.33 ± 0.751
1.042AsnHis: 1.042 ± 0.306
3.287AsnIle: 3.287 ± 0.432
4.811AsnLys: 4.811 ± 0.649
4.891AsnLeu: 4.891 ± 0.67
1.283AsnMet: 1.283 ± 0.302
2.405AsnAsn: 2.405 ± 0.457
1.844AsnPro: 1.844 ± 0.287
1.764AsnGln: 1.764 ± 0.33
2.566AsnArg: 2.566 ± 0.46
2.085AsnSer: 2.085 ± 0.45
3.207AsnThr: 3.207 ± 0.532
3.368AsnVal: 3.368 ± 0.49
0.641AsnTrp: 0.641 ± 0.23
1.924AsnTyr: 1.924 ± 0.436
0.0AsnXaa: 0.0 ± 0.0
Pro
1.604ProAla: 1.604 ± 0.269
0.241ProCys: 0.241 ± 0.168
1.604ProAsp: 1.604 ± 0.432
1.844ProGlu: 1.844 ± 0.435
1.363ProPhe: 1.363 ± 0.328
1.203ProGly: 1.203 ± 0.406
0.802ProHis: 0.802 ± 0.213
2.245ProIle: 2.245 ± 0.496
2.405ProLys: 2.405 ± 0.531
2.245ProLeu: 2.245 ± 0.427
0.802ProMet: 0.802 ± 0.205
1.684ProAsn: 1.684 ± 0.343
0.962ProPro: 0.962 ± 0.329
0.962ProGln: 0.962 ± 0.275
0.401ProArg: 0.401 ± 0.162
1.844ProSer: 1.844 ± 0.284
2.245ProThr: 2.245 ± 0.45
2.085ProVal: 2.085 ± 0.504
0.401ProTrp: 0.401 ± 0.166
1.604ProTyr: 1.604 ± 0.386
0.0ProXaa: 0.0 ± 0.0
Gln
2.726GlnAla: 2.726 ± 0.425
0.08GlnCys: 0.08 ± 0.074
1.523GlnAsp: 1.523 ± 0.327
3.849GlnGlu: 3.849 ± 0.526
1.924GlnPhe: 1.924 ± 0.417
2.566GlnGly: 2.566 ± 0.423
0.722GlnHis: 0.722 ± 0.442
2.967GlnIle: 2.967 ± 0.448
3.929GlnLys: 3.929 ± 0.438
3.127GlnLeu: 3.127 ± 0.54
1.604GlnMet: 1.604 ± 0.356
2.004GlnAsn: 2.004 ± 0.471
1.363GlnPro: 1.363 ± 0.367
2.486GlnGln: 2.486 ± 0.475
1.844GlnArg: 1.844 ± 0.295
2.085GlnSer: 2.085 ± 0.326
2.165GlnThr: 2.165 ± 0.445
1.924GlnVal: 1.924 ± 0.402
0.321GlnTrp: 0.321 ± 0.161
1.924GlnTyr: 1.924 ± 0.326
0.0GlnXaa: 0.0 ± 0.0
Arg
2.085ArgAla: 2.085 ± 0.373
0.561ArgCys: 0.561 ± 0.232
2.726ArgAsp: 2.726 ± 0.462
3.127ArgGlu: 3.127 ± 0.46
2.405ArgPhe: 2.405 ± 0.495
3.127ArgGly: 3.127 ± 0.474
1.042ArgHis: 1.042 ± 0.259
3.528ArgIle: 3.528 ± 0.578
4.33ArgLys: 4.33 ± 0.678
4.089ArgLeu: 4.089 ± 0.559
1.684ArgMet: 1.684 ± 0.344
2.085ArgAsn: 2.085 ± 0.432
1.042ArgPro: 1.042 ± 0.263
1.443ArgGln: 1.443 ± 0.312
1.684ArgArg: 1.684 ± 0.375
1.844ArgSer: 1.844 ± 0.317
2.085ArgThr: 2.085 ± 0.497
3.287ArgVal: 3.287 ± 0.566
0.481ArgTrp: 0.481 ± 0.171
1.684ArgTyr: 1.684 ± 0.523
0.0ArgXaa: 0.0 ± 0.0
Ser
2.967SerAla: 2.967 ± 0.488
0.401SerCys: 0.401 ± 0.157
4.009SerAsp: 4.009 ± 0.501
3.849SerGlu: 3.849 ± 0.444
3.448SerPhe: 3.448 ± 0.52
2.486SerGly: 2.486 ± 0.541
0.561SerHis: 0.561 ± 0.216
4.811SerIle: 4.811 ± 0.688
4.811SerLys: 4.811 ± 0.575
4.169SerLeu: 4.169 ± 0.472
1.764SerMet: 1.764 ± 0.322
3.528SerAsn: 3.528 ± 0.471
1.604SerPro: 1.604 ± 0.28
2.165SerGln: 2.165 ± 0.51
2.405SerArg: 2.405 ± 0.478
3.127SerSer: 3.127 ± 0.564
2.165SerThr: 2.165 ± 0.441
3.849SerVal: 3.849 ± 0.832
0.241SerTrp: 0.241 ± 0.144
2.085SerTyr: 2.085 ± 0.447
0.0SerXaa: 0.0 ± 0.0
Thr
3.287ThrAla: 3.287 ± 0.451
0.241ThrCys: 0.241 ± 0.125
2.967ThrAsp: 2.967 ± 0.567
4.089ThrGlu: 4.089 ± 0.602
2.967ThrPhe: 2.967 ± 0.412
3.368ThrGly: 3.368 ± 0.567
0.802ThrHis: 0.802 ± 0.269
4.65ThrIle: 4.65 ± 0.603
6.013ThrLys: 6.013 ± 0.792
4.971ThrLeu: 4.971 ± 0.787
1.523ThrMet: 1.523 ± 0.344
2.726ThrAsn: 2.726 ± 0.547
2.004ThrPro: 2.004 ± 0.443
2.085ThrGln: 2.085 ± 0.287
1.924ThrArg: 1.924 ± 0.393
1.924ThrSer: 1.924 ± 0.378
2.886ThrThr: 2.886 ± 0.593
4.41ThrVal: 4.41 ± 0.526
0.722ThrTrp: 0.722 ± 0.256
2.085ThrTyr: 2.085 ± 0.496
0.0ThrXaa: 0.0 ± 0.0
Val
4.089ValAla: 4.089 ± 0.511
0.561ValCys: 0.561 ± 0.185
4.089ValAsp: 4.089 ± 0.532
6.254ValGlu: 6.254 ± 0.798
2.405ValPhe: 2.405 ± 0.561
3.768ValGly: 3.768 ± 0.572
1.042ValHis: 1.042 ± 0.26
4.169ValIle: 4.169 ± 0.527
5.292ValLys: 5.292 ± 0.616
4.25ValLeu: 4.25 ± 0.488
1.684ValMet: 1.684 ± 0.349
3.287ValAsn: 3.287 ± 0.393
2.165ValPro: 2.165 ± 0.402
2.967ValGln: 2.967 ± 0.429
3.047ValArg: 3.047 ± 0.58
4.41ValSer: 4.41 ± 0.638
3.929ValThr: 3.929 ± 0.667
4.009ValVal: 4.009 ± 0.599
0.722ValTrp: 0.722 ± 0.247
2.806ValTyr: 2.806 ± 0.454
0.0ValXaa: 0.0 ± 0.0
Trp
0.802TrpAla: 0.802 ± 0.263
0.321TrpCys: 0.321 ± 0.173
1.283TrpAsp: 1.283 ± 0.319
1.283TrpGlu: 1.283 ± 0.316
0.641TrpPhe: 0.641 ± 0.243
0.802TrpGly: 0.802 ± 0.249
0.241TrpHis: 0.241 ± 0.189
0.802TrpIle: 0.802 ± 0.23
1.042TrpLys: 1.042 ± 0.265
1.123TrpLeu: 1.123 ± 0.288
0.321TrpMet: 0.321 ± 0.182
0.802TrpAsn: 0.802 ± 0.207
0.321TrpPro: 0.321 ± 0.171
0.641TrpGln: 0.641 ± 0.223
0.561TrpArg: 0.561 ± 0.207
0.561TrpSer: 0.561 ± 0.203
0.641TrpThr: 0.641 ± 0.198
0.641TrpVal: 0.641 ± 0.259
0.08TrpTrp: 0.08 ± 0.074
0.321TrpTyr: 0.321 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.844TyrAla: 1.844 ± 0.348
0.561TyrCys: 0.561 ± 0.24
1.924TyrAsp: 1.924 ± 0.437
2.646TyrGlu: 2.646 ± 0.44
1.924TyrPhe: 1.924 ± 0.449
2.245TyrGly: 2.245 ± 0.423
0.641TyrHis: 0.641 ± 0.235
2.486TyrIle: 2.486 ± 0.44
2.806TyrLys: 2.806 ± 0.46
3.688TyrLeu: 3.688 ± 0.736
1.203TyrMet: 1.203 ± 0.323
1.764TyrAsn: 1.764 ± 0.389
1.283TyrPro: 1.283 ± 0.4
1.363TyrGln: 1.363 ± 0.332
1.684TyrArg: 1.684 ± 0.368
1.844TyrSer: 1.844 ± 0.318
2.085TyrThr: 2.085 ± 0.464
2.886TyrVal: 2.886 ± 0.534
0.722TyrTrp: 0.722 ± 0.212
1.764TyrTyr: 1.764 ± 0.474
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12473 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski