Pseudoalteromonas piscicida

Taxonomy: cellular organisms; Bacteria

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4134 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F4QCQ3|A0A0F4QCQ3_PSEO7 XRE family transcriptional regulator OS=Pseudoalteromonas piscicida OX=43662 GN=CWB94_14295 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.35KK3 pKa = 9.96HH4 pKa = 6.13FLAAALSLACLAPAAHH20 pKa = 7.38ADD22 pKa = 3.94TVLGLYY28 pKa = 9.94VGAEE32 pKa = 3.98GWDD35 pKa = 3.79TEE37 pKa = 4.75TEE39 pKa = 4.13GSFAEE44 pKa = 4.39KK45 pKa = 11.17GNMQTFNFDD54 pKa = 3.95DD55 pKa = 3.65EE56 pKa = 5.08TFTSFYY62 pKa = 10.95AALEE66 pKa = 4.29HH67 pKa = 6.5PVPLVPNIKK76 pKa = 10.09LRR78 pKa = 11.84YY79 pKa = 8.15TEE81 pKa = 5.37LEE83 pKa = 4.21LNGQTTLTEE92 pKa = 4.12SFDD95 pKa = 4.0FGGQVYY101 pKa = 7.78TVGTDD106 pKa = 2.83ATTNADD112 pKa = 4.38LNHH115 pKa = 6.9IDD117 pKa = 3.26YY118 pKa = 9.35TLYY121 pKa = 11.37YY122 pKa = 10.08EE123 pKa = 5.18VLDD126 pKa = 4.24NDD128 pKa = 5.3LLTLDD133 pKa = 4.35FGVNFKK139 pKa = 10.91QFDD142 pKa = 3.73GSIMVTGTANGQTRR156 pKa = 11.84TEE158 pKa = 4.03EE159 pKa = 4.17VEE161 pKa = 4.12FSGFVPLGYY170 pKa = 10.5LRR172 pKa = 11.84GEE174 pKa = 4.14VGLPLTGLSVFAEE187 pKa = 4.69GSMLAIDD194 pKa = 4.25DD195 pKa = 5.02SKK197 pKa = 11.23IQDD200 pKa = 3.72YY201 pKa = 10.98QIGIAYY207 pKa = 8.88EE208 pKa = 4.86FIDD211 pKa = 4.1NMAVDD216 pKa = 3.99VAVKK220 pKa = 9.76VGYY223 pKa = 10.29RR224 pKa = 11.84SMVLEE229 pKa = 5.26LDD231 pKa = 5.19DD232 pKa = 5.66IDD234 pKa = 6.59DD235 pKa = 4.39IYY237 pKa = 11.42TDD239 pKa = 4.74LDD241 pKa = 3.62VSGPFAGIQLHH252 pKa = 5.95FF253 pKa = 4.53

Molecular weight: 27.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F4QCV0|A0A0F4QCV0_PSEO7 DeoR faimly transcriptional regulator OS=Pseudoalteromonas piscicida OX=43662 GN=CWB94_13985 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 8.96VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4134

0

4134

1440159

44

4018

348.4

38.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.949 ± 0.034

1.011 ± 0.012

5.46 ± 0.027

6.353 ± 0.038

4.261 ± 0.024

6.404 ± 0.038

2.322 ± 0.02

6.123 ± 0.029

5.474 ± 0.034

10.617 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.016

4.364 ± 0.025

3.759 ± 0.021

5.02 ± 0.034

4.202 ± 0.022

6.747 ± 0.036

5.414 ± 0.03

6.831 ± 0.028

1.167 ± 0.014

3.236 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski