Amino acid dipepetide frequency for Acinetobacter phage AbTJ

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.362AlaAla: 5.362 ± 0.792
0.604AlaCys: 0.604 ± 0.217
4.304AlaAsp: 4.304 ± 0.58
4.531AlaGlu: 4.531 ± 0.599
2.19AlaPhe: 2.19 ± 0.427
4.984AlaGly: 4.984 ± 0.724
1.586AlaHis: 1.586 ± 0.408
5.588AlaIle: 5.588 ± 0.746
5.664AlaLys: 5.664 ± 0.815
6.948AlaLeu: 6.948 ± 0.674
2.266AlaMet: 2.266 ± 0.357
4.229AlaAsn: 4.229 ± 0.549
2.19AlaPro: 2.19 ± 0.31
2.945AlaGln: 2.945 ± 0.472
3.172AlaArg: 3.172 ± 0.442
3.7AlaSer: 3.7 ± 0.553
4.304AlaThr: 4.304 ± 0.585
5.513AlaVal: 5.513 ± 0.621
0.906AlaTrp: 0.906 ± 0.25
2.19AlaTyr: 2.19 ± 0.492
0.0AlaXaa: 0.0 ± 0.0
Cys
0.453CysAla: 0.453 ± 0.196
0.227CysCys: 0.227 ± 0.116
0.906CysAsp: 0.906 ± 0.291
0.68CysGlu: 0.68 ± 0.253
0.68CysPhe: 0.68 ± 0.186
0.831CysGly: 0.831 ± 0.262
0.0CysHis: 0.0 ± 0.0
0.906CysIle: 0.906 ± 0.256
1.057CysLys: 1.057 ± 0.283
0.755CysLeu: 0.755 ± 0.238
0.076CysMet: 0.076 ± 0.062
0.604CysAsn: 0.604 ± 0.188
0.378CysPro: 0.378 ± 0.153
0.076CysGln: 0.076 ± 0.082
0.831CysArg: 0.831 ± 0.192
0.529CysSer: 0.529 ± 0.195
0.831CysThr: 0.831 ± 0.28
0.302CysVal: 0.302 ± 0.127
0.076CysTrp: 0.076 ± 0.071
0.378CysTyr: 0.378 ± 0.156
0.0CysXaa: 0.0 ± 0.0
Asp
4.078AspAla: 4.078 ± 0.567
0.906AspCys: 0.906 ± 0.252
3.398AspAsp: 3.398 ± 0.705
4.153AspGlu: 4.153 ± 0.542
2.417AspPhe: 2.417 ± 0.367
5.06AspGly: 5.06 ± 0.708
1.133AspHis: 1.133 ± 0.261
4.002AspIle: 4.002 ± 0.655
3.172AspLys: 3.172 ± 0.378
5.211AspLeu: 5.211 ± 0.645
1.51AspMet: 1.51 ± 0.296
2.719AspAsn: 2.719 ± 0.374
2.266AspPro: 2.266 ± 0.384
2.794AspGln: 2.794 ± 0.519
2.417AspArg: 2.417 ± 0.393
4.153AspSer: 4.153 ± 0.672
3.096AspThr: 3.096 ± 0.388
4.909AspVal: 4.909 ± 0.631
0.831AspTrp: 0.831 ± 0.22
1.963AspTyr: 1.963 ± 0.341
0.0AspXaa: 0.0 ± 0.0
Glu
4.682GluAla: 4.682 ± 0.565
0.529GluCys: 0.529 ± 0.221
4.229GluAsp: 4.229 ± 0.792
4.909GluGlu: 4.909 ± 0.76
3.247GluPhe: 3.247 ± 0.478
3.776GluGly: 3.776 ± 0.51
0.831GluHis: 0.831 ± 0.27
5.06GluIle: 5.06 ± 0.67
4.682GluLys: 4.682 ± 0.54
6.872GluLeu: 6.872 ± 0.648
1.51GluMet: 1.51 ± 0.386
3.096GluAsn: 3.096 ± 0.463
1.737GluPro: 1.737 ± 0.496
3.096GluGln: 3.096 ± 0.607
2.643GluArg: 2.643 ± 0.369
4.002GluSer: 4.002 ± 0.579
3.398GluThr: 3.398 ± 0.425
4.304GluVal: 4.304 ± 0.541
1.208GluTrp: 1.208 ± 0.385
2.19GluTyr: 2.19 ± 0.399
0.0GluXaa: 0.0 ± 0.0
Phe
3.625PheAla: 3.625 ± 0.435
0.529PheCys: 0.529 ± 0.215
2.945PheAsp: 2.945 ± 0.54
2.266PheGlu: 2.266 ± 0.474
1.359PhePhe: 1.359 ± 0.357
2.87PheGly: 2.87 ± 0.418
0.68PheHis: 0.68 ± 0.226
3.172PheIle: 3.172 ± 0.487
2.945PheLys: 2.945 ± 0.383
2.492PheLeu: 2.492 ± 0.434
0.604PheMet: 0.604 ± 0.157
2.568PheAsn: 2.568 ± 0.464
1.284PhePro: 1.284 ± 0.257
1.359PheGln: 1.359 ± 0.298
1.586PheArg: 1.586 ± 0.326
2.794PheSer: 2.794 ± 0.474
2.114PheThr: 2.114 ± 0.383
2.341PheVal: 2.341 ± 0.507
0.529PheTrp: 0.529 ± 0.176
2.114PheTyr: 2.114 ± 0.412
0.0PheXaa: 0.0 ± 0.0
Gly
4.833GlyAla: 4.833 ± 0.63
0.378GlyCys: 0.378 ± 0.152
4.531GlyAsp: 4.531 ± 0.67
4.304GlyGlu: 4.304 ± 0.469
3.549GlyPhe: 3.549 ± 0.555
5.286GlyGly: 5.286 ± 0.705
0.755GlyHis: 0.755 ± 0.24
4.229GlyIle: 4.229 ± 0.651
4.38GlyLys: 4.38 ± 0.664
5.135GlyLeu: 5.135 ± 0.584
1.963GlyMet: 1.963 ± 0.419
2.719GlyAsn: 2.719 ± 0.389
1.51GlyPro: 1.51 ± 0.308
2.87GlyGln: 2.87 ± 0.511
3.247GlyArg: 3.247 ± 0.511
3.927GlySer: 3.927 ± 0.5
3.7GlyThr: 3.7 ± 0.642
4.909GlyVal: 4.909 ± 0.66
1.51GlyTrp: 1.51 ± 0.336
2.794GlyTyr: 2.794 ± 0.415
0.0GlyXaa: 0.0 ± 0.0
His
0.831HisAla: 0.831 ± 0.302
0.302HisCys: 0.302 ± 0.154
0.831HisAsp: 0.831 ± 0.236
1.661HisGlu: 1.661 ± 0.339
0.453HisPhe: 0.453 ± 0.184
0.755HisGly: 0.755 ± 0.236
0.302HisHis: 0.302 ± 0.135
1.661HisIle: 1.661 ± 0.328
1.435HisLys: 1.435 ± 0.322
1.737HisLeu: 1.737 ± 0.329
0.453HisMet: 0.453 ± 0.252
0.529HisAsn: 0.529 ± 0.197
0.529HisPro: 0.529 ± 0.199
0.529HisGln: 0.529 ± 0.15
0.604HisArg: 0.604 ± 0.183
1.057HisSer: 1.057 ± 0.281
0.68HisThr: 0.68 ± 0.219
0.755HisVal: 0.755 ± 0.244
0.227HisTrp: 0.227 ± 0.136
0.831HisTyr: 0.831 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
5.211IleAla: 5.211 ± 0.577
0.831IleCys: 0.831 ± 0.284
4.984IleAsp: 4.984 ± 0.535
4.607IleGlu: 4.607 ± 0.64
2.039IlePhe: 2.039 ± 0.299
4.38IleGly: 4.38 ± 0.604
1.208IleHis: 1.208 ± 0.274
3.474IleIle: 3.474 ± 0.523
4.531IleLys: 4.531 ± 0.52
4.304IleLeu: 4.304 ± 0.557
0.755IleMet: 0.755 ± 0.221
4.758IleAsn: 4.758 ± 0.641
2.568IlePro: 2.568 ± 0.515
2.945IleGln: 2.945 ± 0.417
2.87IleArg: 2.87 ± 0.479
4.607IleSer: 4.607 ± 0.459
4.758IleThr: 4.758 ± 0.596
3.851IleVal: 3.851 ± 0.502
0.831IleTrp: 0.831 ± 0.247
1.963IleTyr: 1.963 ± 0.353
0.0IleXaa: 0.0 ± 0.0
Lys
5.815LysAla: 5.815 ± 0.751
0.831LysCys: 0.831 ± 0.305
3.851LysAsp: 3.851 ± 0.534
5.135LysGlu: 5.135 ± 0.719
2.568LysPhe: 2.568 ± 0.387
4.229LysGly: 4.229 ± 0.58
1.435LysHis: 1.435 ± 0.408
4.078LysIle: 4.078 ± 0.562
5.513LysLys: 5.513 ± 0.798
6.797LysLeu: 6.797 ± 0.698
1.359LysMet: 1.359 ± 0.327
3.474LysAsn: 3.474 ± 0.587
2.719LysPro: 2.719 ± 0.571
4.078LysGln: 4.078 ± 0.463
3.776LysArg: 3.776 ± 0.651
4.984LysSer: 4.984 ± 0.689
4.078LysThr: 4.078 ± 0.706
4.758LysVal: 4.758 ± 0.621
1.359LysTrp: 1.359 ± 0.275
1.661LysTyr: 1.661 ± 0.286
0.0LysXaa: 0.0 ± 0.0
Leu
6.192LeuAla: 6.192 ± 0.976
0.453LeuCys: 0.453 ± 0.179
5.664LeuAsp: 5.664 ± 0.635
4.531LeuGlu: 4.531 ± 0.597
2.417LeuPhe: 2.417 ± 0.347
5.135LeuGly: 5.135 ± 0.522
1.435LeuHis: 1.435 ± 0.334
5.362LeuIle: 5.362 ± 0.645
8.609LeuLys: 8.609 ± 0.94
7.325LeuLeu: 7.325 ± 0.968
2.266LeuMet: 2.266 ± 0.391
5.815LeuAsn: 5.815 ± 0.568
3.247LeuPro: 3.247 ± 0.473
3.323LeuGln: 3.323 ± 0.486
4.304LeuArg: 4.304 ± 0.456
5.815LeuSer: 5.815 ± 0.884
5.362LeuThr: 5.362 ± 0.611
5.286LeuVal: 5.286 ± 0.692
0.831LeuTrp: 0.831 ± 0.317
3.021LeuTyr: 3.021 ± 0.41
0.0LeuXaa: 0.0 ± 0.0
Met
1.888MetAla: 1.888 ± 0.356
0.378MetCys: 0.378 ± 0.142
1.359MetAsp: 1.359 ± 0.308
1.057MetGlu: 1.057 ± 0.287
0.453MetPhe: 0.453 ± 0.173
1.284MetGly: 1.284 ± 0.293
0.529MetHis: 0.529 ± 0.197
1.51MetIle: 1.51 ± 0.341
1.737MetLys: 1.737 ± 0.305
1.586MetLeu: 1.586 ± 0.308
0.378MetMet: 0.378 ± 0.144
1.284MetAsn: 1.284 ± 0.284
1.586MetPro: 1.586 ± 0.316
0.68MetGln: 0.68 ± 0.236
1.359MetArg: 1.359 ± 0.276
1.963MetSer: 1.963 ± 0.307
2.039MetThr: 2.039 ± 0.385
1.208MetVal: 1.208 ± 0.309
0.076MetTrp: 0.076 ± 0.083
0.982MetTyr: 0.982 ± 0.273
0.0MetXaa: 0.0 ± 0.0
Asn
4.38AsnAla: 4.38 ± 0.586
1.208AsnCys: 1.208 ± 0.329
2.417AsnAsp: 2.417 ± 0.531
3.549AsnGlu: 3.549 ± 0.45
2.266AsnPhe: 2.266 ± 0.377
4.909AsnGly: 4.909 ± 0.562
1.435AsnHis: 1.435 ± 0.299
3.172AsnIle: 3.172 ± 0.485
3.398AsnLys: 3.398 ± 0.429
3.7AsnLeu: 3.7 ± 0.441
1.057AsnMet: 1.057 ± 0.264
3.474AsnAsn: 3.474 ± 0.507
2.266AsnPro: 2.266 ± 0.361
2.492AsnGln: 2.492 ± 0.547
2.19AsnArg: 2.19 ± 0.382
3.172AsnSer: 3.172 ± 0.479
4.002AsnThr: 4.002 ± 0.477
2.19AsnVal: 2.19 ± 0.298
0.906AsnTrp: 0.906 ± 0.28
1.586AsnTyr: 1.586 ± 0.314
0.0AsnXaa: 0.0 ± 0.0
Pro
3.021ProAla: 3.021 ± 0.465
0.151ProCys: 0.151 ± 0.108
3.096ProAsp: 3.096 ± 0.511
2.945ProGlu: 2.945 ± 0.498
1.208ProPhe: 1.208 ± 0.271
1.435ProGly: 1.435 ± 0.275
0.68ProHis: 0.68 ± 0.207
2.568ProIle: 2.568 ± 0.374
2.719ProLys: 2.719 ± 0.449
3.323ProLeu: 3.323 ± 0.658
0.529ProMet: 0.529 ± 0.203
1.359ProAsn: 1.359 ± 0.296
1.51ProPro: 1.51 ± 0.29
1.888ProGln: 1.888 ± 0.461
1.133ProArg: 1.133 ± 0.267
2.719ProSer: 2.719 ± 0.501
2.643ProThr: 2.643 ± 0.535
3.096ProVal: 3.096 ± 0.568
0.453ProTrp: 0.453 ± 0.209
0.906ProTyr: 0.906 ± 0.229
0.0ProXaa: 0.0 ± 0.0
Gln
3.021GlnAla: 3.021 ± 0.408
0.302GlnCys: 0.302 ± 0.156
1.208GlnAsp: 1.208 ± 0.257
3.096GlnGlu: 3.096 ± 0.525
2.039GlnPhe: 2.039 ± 0.375
2.039GlnGly: 2.039 ± 0.417
0.302GlnHis: 0.302 ± 0.14
3.323GlnIle: 3.323 ± 0.554
3.172GlnLys: 3.172 ± 0.472
4.531GlnLeu: 4.531 ± 0.585
0.906GlnMet: 0.906 ± 0.227
2.492GlnAsn: 2.492 ± 0.357
1.661GlnPro: 1.661 ± 0.366
2.266GlnGln: 2.266 ± 0.42
1.661GlnArg: 1.661 ± 0.309
3.927GlnSer: 3.927 ± 0.519
2.643GlnThr: 2.643 ± 0.392
2.417GlnVal: 2.417 ± 0.357
0.831GlnTrp: 0.831 ± 0.199
1.812GlnTyr: 1.812 ± 0.399
0.0GlnXaa: 0.0 ± 0.0
Arg
2.945ArgAla: 2.945 ± 0.396
0.755ArgCys: 0.755 ± 0.233
3.172ArgAsp: 3.172 ± 0.487
2.87ArgGlu: 2.87 ± 0.405
2.19ArgPhe: 2.19 ± 0.382
2.794ArgGly: 2.794 ± 0.55
0.529ArgHis: 0.529 ± 0.165
3.096ArgIle: 3.096 ± 0.43
2.794ArgLys: 2.794 ± 0.417
4.229ArgLeu: 4.229 ± 0.577
1.284ArgMet: 1.284 ± 0.334
2.568ArgAsn: 2.568 ± 0.403
1.435ArgPro: 1.435 ± 0.308
2.266ArgGln: 2.266 ± 0.368
2.341ArgArg: 2.341 ± 0.407
2.568ArgSer: 2.568 ± 0.392
2.643ArgThr: 2.643 ± 0.393
2.039ArgVal: 2.039 ± 0.305
0.68ArgTrp: 0.68 ± 0.208
1.586ArgTyr: 1.586 ± 0.449
0.0ArgXaa: 0.0 ± 0.0
Ser
3.7SerAla: 3.7 ± 0.529
0.302SerCys: 0.302 ± 0.147
3.549SerAsp: 3.549 ± 0.497
4.531SerGlu: 4.531 ± 0.744
4.153SerPhe: 4.153 ± 0.545
4.531SerGly: 4.531 ± 0.683
1.057SerHis: 1.057 ± 0.305
3.323SerIle: 3.323 ± 0.484
5.06SerLys: 5.06 ± 0.666
5.966SerLeu: 5.966 ± 0.655
1.963SerMet: 1.963 ± 0.375
3.021SerAsn: 3.021 ± 0.602
2.87SerPro: 2.87 ± 0.36
2.719SerGln: 2.719 ± 0.448
3.021SerArg: 3.021 ± 0.556
4.38SerSer: 4.38 ± 0.692
4.229SerThr: 4.229 ± 0.512
4.984SerVal: 4.984 ± 0.603
0.755SerTrp: 0.755 ± 0.237
1.963SerTyr: 1.963 ± 0.391
0.0SerXaa: 0.0 ± 0.0
Thr
4.833ThrAla: 4.833 ± 0.672
0.529ThrCys: 0.529 ± 0.196
3.776ThrAsp: 3.776 ± 0.483
3.474ThrGlu: 3.474 ± 0.49
2.492ThrPhe: 2.492 ± 0.542
4.153ThrGly: 4.153 ± 0.666
0.906ThrHis: 0.906 ± 0.257
4.078ThrIle: 4.078 ± 0.442
4.531ThrLys: 4.531 ± 0.734
5.588ThrLeu: 5.588 ± 0.741
1.586ThrMet: 1.586 ± 0.334
2.643ThrAsn: 2.643 ± 0.389
4.078ThrPro: 4.078 ± 0.453
2.417ThrGln: 2.417 ± 0.37
2.039ThrArg: 2.039 ± 0.344
2.719ThrSer: 2.719 ± 0.441
4.002ThrThr: 4.002 ± 0.524
4.456ThrVal: 4.456 ± 0.574
0.906ThrTrp: 0.906 ± 0.245
2.417ThrTyr: 2.417 ± 0.396
0.0ThrXaa: 0.0 ± 0.0
Val
4.833ValAla: 4.833 ± 0.666
0.529ValCys: 0.529 ± 0.207
3.851ValAsp: 3.851 ± 0.533
5.211ValGlu: 5.211 ± 0.657
2.417ValPhe: 2.417 ± 0.526
4.002ValGly: 4.002 ± 0.572
0.982ValHis: 0.982 ± 0.336
3.549ValIle: 3.549 ± 0.495
4.456ValLys: 4.456 ± 0.546
5.513ValLeu: 5.513 ± 0.508
1.888ValMet: 1.888 ± 0.453
3.398ValAsn: 3.398 ± 0.565
2.417ValPro: 2.417 ± 0.458
2.492ValGln: 2.492 ± 0.356
2.945ValArg: 2.945 ± 0.462
5.739ValSer: 5.739 ± 0.656
4.078ValThr: 4.078 ± 0.53
4.531ValVal: 4.531 ± 0.59
0.453ValTrp: 0.453 ± 0.191
1.586ValTyr: 1.586 ± 0.399
0.0ValXaa: 0.0 ± 0.0
Trp
1.133TrpAla: 1.133 ± 0.277
0.302TrpCys: 0.302 ± 0.156
0.378TrpAsp: 0.378 ± 0.182
0.529TrpGlu: 0.529 ± 0.185
0.604TrpPhe: 0.604 ± 0.181
0.982TrpGly: 0.982 ± 0.242
0.0TrpHis: 0.0 ± 0.0
0.831TrpIle: 0.831 ± 0.217
0.68TrpLys: 0.68 ± 0.184
1.359TrpLeu: 1.359 ± 0.287
0.227TrpMet: 0.227 ± 0.134
1.057TrpAsn: 1.057 ± 0.307
0.378TrpPro: 0.378 ± 0.159
0.68TrpGln: 0.68 ± 0.278
0.755TrpArg: 0.755 ± 0.239
1.208TrpSer: 1.208 ± 0.298
0.906TrpThr: 0.906 ± 0.275
1.208TrpVal: 1.208 ± 0.211
0.151TrpTrp: 0.151 ± 0.097
0.453TrpTyr: 0.453 ± 0.166
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.341TyrAla: 2.341 ± 0.436
0.529TyrCys: 0.529 ± 0.189
1.737TyrAsp: 1.737 ± 0.412
1.963TyrGlu: 1.963 ± 0.381
1.435TyrPhe: 1.435 ± 0.307
3.096TyrGly: 3.096 ± 0.56
0.378TyrHis: 0.378 ± 0.177
2.492TyrIle: 2.492 ± 0.439
2.039TyrLys: 2.039 ± 0.339
3.021TyrLeu: 3.021 ± 0.456
0.68TyrMet: 0.68 ± 0.228
1.963TyrAsn: 1.963 ± 0.36
0.755TyrPro: 0.755 ± 0.216
1.737TyrGln: 1.737 ± 0.39
1.888TyrArg: 1.888 ± 0.331
2.114TyrSer: 2.114 ± 0.457
2.114TyrThr: 2.114 ± 0.364
1.812TyrVal: 1.812 ± 0.345
0.302TyrTrp: 0.302 ± 0.155
1.133TyrTyr: 1.133 ± 0.293
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski