Plasmodium knowlesi (strain H)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Plasmodium; Plasmodium (Plasmodium); Plasmodium knowlesi

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5339 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A679KWN9|A0A679KWN9_PLAKH Uncharacterized protein OS=Plasmodium knowlesi (strain H) OX=5851 GN=PKNH_0838400 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 4.35HH3 pKa = 6.41QPPRR7 pKa = 11.84RR8 pKa = 11.84NTNPFIDD15 pKa = 3.9STEE18 pKa = 3.97DD19 pKa = 3.45SINGSDD25 pKa = 3.9TPNVLPVDD33 pKa = 4.89LNSQDD38 pKa = 4.0FGINQWGVGVTEE50 pKa = 4.42GDD52 pKa = 3.76GFSSNVDD59 pKa = 3.23YY60 pKa = 11.93NMGLNSAANGSANGASNGATNGLPNVAPNAAPNAAPNPFANATPNPFANTTPNPFTNTTPNPFTNTTPNPFANTTPNPFANTTPNPFANTTPNPFTNTTPNPFTNTTPNAVPNYY174 pKa = 9.23DD175 pKa = 3.53FNYY178 pKa = 9.1GANSAAQFGQNFNPHH193 pKa = 6.3IGVPSHH199 pKa = 7.06DD200 pKa = 3.78QGNMTSYY207 pKa = 11.01LVDD210 pKa = 2.82STMFNFIQAVVLATTAFLSAVRR232 pKa = 11.84MNRR235 pKa = 11.84HH236 pKa = 4.98MVQPTITKK244 pKa = 10.29NEE246 pKa = 3.47FDD248 pKa = 3.42VAVFHH253 pKa = 6.83NFPTQEE259 pKa = 4.09TTPSGEE265 pKa = 4.12SEE267 pKa = 4.08KK268 pKa = 10.98EE269 pKa = 3.82EE270 pKa = 4.36EE271 pKa = 3.97ISYY274 pKa = 10.91SPLHH278 pKa = 6.51NEE280 pKa = 4.08EE281 pKa = 5.33NNDD284 pKa = 3.88QSDD287 pKa = 3.95TLPLHH292 pKa = 5.76THH294 pKa = 5.72TEE296 pKa = 4.08VHH298 pKa = 6.81PEE300 pKa = 3.96TNTEE304 pKa = 4.16TNTVTNAEE312 pKa = 4.46TNTVTNAEE320 pKa = 4.42TNAEE324 pKa = 4.51TNPEE328 pKa = 4.12IYY330 pKa = 9.97PLLGDD335 pKa = 3.07VDD337 pKa = 4.43YY338 pKa = 11.0EE339 pKa = 4.41HH340 pKa = 7.37EE341 pKa = 4.61PFLPDD346 pKa = 3.59EE347 pKa = 4.56SEE349 pKa = 4.05GDD351 pKa = 3.46IFDD354 pKa = 4.77EE355 pKa = 4.38EE356 pKa = 5.73AEE358 pKa = 4.28DD359 pKa = 4.5SPDD362 pKa = 3.24EE363 pKa = 4.41GEE365 pKa = 4.58YY366 pKa = 10.31EE367 pKa = 4.05VHH369 pKa = 6.31EE370 pKa = 4.6AEE372 pKa = 4.89IQNLMNISYY381 pKa = 9.38DD382 pKa = 3.48IPADD386 pKa = 3.92RR387 pKa = 11.84YY388 pKa = 8.7LTNPFDD394 pKa = 4.03EE395 pKa = 5.45LSNQAGASSGARR407 pKa = 11.84RR408 pKa = 11.84RR409 pKa = 11.84TYY411 pKa = 10.12QPPNYY416 pKa = 10.04GEE418 pKa = 4.32VYY420 pKa = 10.38EE421 pKa = 4.68GAQFVDD427 pKa = 4.59KK428 pKa = 11.12YY429 pKa = 11.21QMSNVGPYY437 pKa = 10.2FINNGNRR444 pKa = 11.84SRR446 pKa = 11.84RR447 pKa = 11.84SGNSEE452 pKa = 4.0SNNYY456 pKa = 9.56GYY458 pKa = 11.46AHH460 pKa = 7.44APYY463 pKa = 10.74ADD465 pKa = 3.74IFRR468 pKa = 11.84HH469 pKa = 5.21PNYY472 pKa = 10.68ANMYY476 pKa = 8.33RR477 pKa = 11.84ATNYY481 pKa = 10.31VMDD484 pKa = 4.57SFLSGTNEE492 pKa = 3.65NTLEE496 pKa = 4.57GIRR499 pKa = 11.84DD500 pKa = 3.84SCSHH504 pKa = 6.43NSADD508 pKa = 4.64DD509 pKa = 3.67SGGANYY515 pKa = 10.48SGGANYY521 pKa = 10.45SGGANYY527 pKa = 10.45SGGANYY533 pKa = 10.67SGDD536 pKa = 3.89DD537 pKa = 3.94NNSGGANYY545 pKa = 10.42SGGANYY551 pKa = 10.62SGDD554 pKa = 3.86ANDD557 pKa = 4.37SGGANYY563 pKa = 10.64SGDD566 pKa = 3.86ANDD569 pKa = 4.37SGGANYY575 pKa = 10.64SGDD578 pKa = 3.86ANDD581 pKa = 4.37SGGANYY587 pKa = 10.64SGDD590 pKa = 3.87ANDD593 pKa = 5.45SGDD596 pKa = 3.7ANNSGGANVSSGSANIGEE614 pKa = 4.16QNIDD618 pKa = 3.05NGYY621 pKa = 10.4DD622 pKa = 3.38DD623 pKa = 5.74SEE625 pKa = 4.44FAHH628 pKa = 6.9TYY630 pKa = 9.28QGNNYY635 pKa = 8.98EE636 pKa = 4.19ITDD639 pKa = 3.87EE640 pKa = 4.95GEE642 pKa = 4.02VADD645 pKa = 4.09PFEE648 pKa = 5.08NVGIDD653 pKa = 3.04NGFDD657 pKa = 3.0FGSYY661 pKa = 9.67EE662 pKa = 4.24NEE664 pKa = 4.02DD665 pKa = 3.88ANGGEE670 pKa = 4.41ATTTTTGSTSQGHH683 pKa = 5.22TVDD686 pKa = 4.91ADD688 pKa = 4.15GITNGDD694 pKa = 3.62VEE696 pKa = 4.88IDD698 pKa = 3.34EE699 pKa = 4.88GDD701 pKa = 3.4NGFGNTEE708 pKa = 3.53KK709 pKa = 10.79HH710 pKa = 5.88EE711 pKa = 4.43PSSEE715 pKa = 3.94KK716 pKa = 10.3TFLTEE721 pKa = 3.88PVKK724 pKa = 10.16NTPTQNDD731 pKa = 4.73TINDD735 pKa = 3.68DD736 pKa = 3.78HH737 pKa = 7.35PSLEE741 pKa = 4.37EE742 pKa = 3.81INVEE746 pKa = 3.96EE747 pKa = 4.35KK748 pKa = 10.78QNSEE752 pKa = 3.99VSEE755 pKa = 4.05QSQPMGHH762 pKa = 7.45PSNDD766 pKa = 3.48DD767 pKa = 3.24DD768 pKa = 6.54DD769 pKa = 5.05EE770 pKa = 5.16FSEE773 pKa = 4.49YY774 pKa = 10.4EE775 pKa = 4.12KK776 pKa = 11.18YY777 pKa = 11.08VYY779 pKa = 8.73TQSGRR784 pKa = 11.84EE785 pKa = 3.91DD786 pKa = 3.09QKK788 pKa = 11.58NYY790 pKa = 9.5NNIMGSYY797 pKa = 8.55LKK799 pKa = 10.26KK800 pKa = 9.85YY801 pKa = 10.47KK802 pKa = 10.13KK803 pKa = 10.29VSEE806 pKa = 4.04NGEE809 pKa = 3.93NEE811 pKa = 3.88KK812 pKa = 11.19AEE814 pKa = 4.21NGVGSDD820 pKa = 3.18AFSPQDD826 pKa = 3.71EE827 pKa = 4.71EE828 pKa = 5.82SPDD831 pKa = 3.41DD832 pKa = 3.75TFPGEE837 pKa = 4.22EE838 pKa = 4.72KK839 pKa = 10.95FNDD842 pKa = 3.77DD843 pKa = 3.64

Molecular weight: 90.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384LD56|A0A384LD56_PLAKH HSP40 subfamily A putative OS=Plasmodium knowlesi (strain H) OX=5851 GN=PKNH_0424600 PE=4 SV=1
MM1 pKa = 7.28AHH3 pKa = 6.31GASRR7 pKa = 11.84YY8 pKa = 8.75KK9 pKa = 10.48KK10 pKa = 10.3SRR12 pKa = 11.84AKK14 pKa = 9.88MRR16 pKa = 11.84WKK18 pKa = 9.16WKK20 pKa = 9.29KK21 pKa = 9.72KK22 pKa = 6.67RR23 pKa = 11.84TRR25 pKa = 11.84RR26 pKa = 11.84LQKK29 pKa = 9.69KK30 pKa = 7.67RR31 pKa = 11.84RR32 pKa = 11.84KK33 pKa = 8.24MRR35 pKa = 11.84QRR37 pKa = 11.84SRR39 pKa = 3.22

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5339

0

5339

3890322

35

10549

728.7

83.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.921 ± 0.023

1.973 ± 0.019

5.778 ± 0.023

7.725 ± 0.039

4.305 ± 0.029

5.644 ± 0.038

2.565 ± 0.015

6.439 ± 0.034

9.698 ± 0.04

8.106 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.012

8.284 ± 0.044

3.032 ± 0.021

3.246 ± 0.015

4.477 ± 0.029

7.763 ± 0.029

4.739 ± 0.022

5.115 ± 0.02

0.688 ± 0.01

4.177 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski