Novosphingobium sp. MBES04

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4731 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S6WWR3|A0A0S6WWR3_9SPHN Regulatory protein RecX OS=Novosphingobium sp. MBES04 OX=1206458 GN=MBENS4_2861 PE=3 SV=1
MM1 pKa = 7.72LLGCSALALAGCGADD16 pKa = 5.41DD17 pKa = 4.13IASPGAGSVVVNNGGGTPTPTPTPTTGIIEE47 pKa = 4.36AAAEE51 pKa = 4.28CPTFNATGGLSNDD64 pKa = 3.69GTIEE68 pKa = 4.21DD69 pKa = 4.57PNGNSWRR76 pKa = 11.84ICTLPALVDD85 pKa = 4.01ASSSLPNEE93 pKa = 3.93AGVLYY98 pKa = 10.16RR99 pKa = 11.84INGRR103 pKa = 11.84VDD105 pKa = 3.16VGCDD109 pKa = 2.96GGFSVPSSGSPYY121 pKa = 7.9TTTTASCLNAGISSLTSDD139 pKa = 3.55TSVEE143 pKa = 3.97LTIDD147 pKa = 3.41PGVIVYY153 pKa = 10.44AEE155 pKa = 4.05NAADD159 pKa = 4.04PAWLAVNRR167 pKa = 11.84GNTIQANGTASSPIVFTSRR186 pKa = 11.84QNVVGSATDD195 pKa = 3.06SSDD198 pKa = 3.66RR199 pKa = 11.84QWGGIVLLGRR209 pKa = 11.84GIITDD214 pKa = 4.03CTAGGSVATDD224 pKa = 3.09DD225 pKa = 4.28CQRR228 pKa = 11.84EE229 pKa = 4.36TEE231 pKa = 4.49GAATPATFGGRR242 pKa = 11.84NNGYY246 pKa = 9.0SAGSMRR252 pKa = 11.84YY253 pKa = 7.87VQIRR257 pKa = 11.84YY258 pKa = 9.22SGYY261 pKa = 10.37NLAPDD266 pKa = 4.93AEE268 pKa = 4.54LQSLTGGGLGTGTTLDD284 pKa = 3.91YY285 pKa = 10.8IQTVNSSDD293 pKa = 3.81DD294 pKa = 3.4GSEE297 pKa = 4.15FFGGAVNMKK306 pKa = 10.27HH307 pKa = 5.88YY308 pKa = 10.43IAVNADD314 pKa = 3.88DD315 pKa = 6.0DD316 pKa = 5.17SLDD319 pKa = 3.46TDD321 pKa = 3.86TGLKK325 pKa = 10.94GNFQYY330 pKa = 11.62LLLLQRR336 pKa = 11.84AGAGDD341 pKa = 3.97AFFEE345 pKa = 4.49IDD347 pKa = 3.75SNNNINSDD355 pKa = 3.47PADD358 pKa = 3.89RR359 pKa = 11.84QRR361 pKa = 11.84STFANFTAIQSGNRR375 pKa = 11.84PDD377 pKa = 4.19NSDD380 pKa = 3.27EE381 pKa = 3.94ASILVRR387 pKa = 11.84GDD389 pKa = 3.03ADD391 pKa = 3.49INFVNGIINTPSNEE405 pKa = 4.26CIRR408 pKa = 11.84VDD410 pKa = 3.07GRR412 pKa = 11.84TATGSATFTANSVVMTCGDD431 pKa = 3.63TGPFLSTGSQYY442 pKa = 11.43SGTNTAANMFNAGTNNNASITSTLTSTFVNGSNEE476 pKa = 4.35SGVVAYY482 pKa = 10.51ANIASFSSFFDD493 pKa = 3.54VVDD496 pKa = 4.92DD497 pKa = 4.21IGAVRR502 pKa = 11.84DD503 pKa = 5.18ANDD506 pKa = 2.47TWYY509 pKa = 10.88RR510 pKa = 11.84GWTCDD515 pKa = 4.03NATADD520 pKa = 3.84FGTGSLCTGLPVAA533 pKa = 5.41

Molecular weight: 54.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S6WW08|A0A0S6WW08_9SPHN Pyrimidine monooxygenase RutA OS=Novosphingobium sp. MBES04 OX=1206458 GN=rutA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4728

3

4731

1456332

26

1928

307.8

33.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.005 ± 0.059

0.859 ± 0.012

5.809 ± 0.027

6.056 ± 0.033

3.558 ± 0.026

8.888 ± 0.035

2.035 ± 0.017

4.674 ± 0.025

2.891 ± 0.026

9.965 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.483 ± 0.018

2.508 ± 0.021

5.299 ± 0.027

3.167 ± 0.02

7.175 ± 0.04

5.398 ± 0.03

5.444 ± 0.026

7.032 ± 0.031

1.453 ± 0.015

2.301 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski