Cyberlindnera fabianii (Yeast) (Hansenula fabianii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota;

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5507 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061AVQ0|A0A061AVQ0_CYBFA Casein kinase II subunit beta OS=Cyberlindnera fabianii OX=36022 GN=BON22_3797 PE=3 SV=1
MM1 pKa = 7.52RR2 pKa = 11.84TTTTTNLLASLAWLATTVIAADD24 pKa = 4.15LTPVADD30 pKa = 4.38LFYY33 pKa = 11.02SRR35 pKa = 11.84TSDD38 pKa = 3.34FPLAVEE44 pKa = 4.73LKK46 pKa = 10.65VGSPQQKK53 pKa = 10.19LLAQLDD59 pKa = 4.13TQSRR63 pKa = 11.84DD64 pKa = 3.01ILLSTEE70 pKa = 3.75YY71 pKa = 10.69DD72 pKa = 3.33AVSSIFDD79 pKa = 3.63TNSFNFNADD88 pKa = 3.13SSSSFSTFSGTSWGSDD104 pKa = 2.94NIGFRR109 pKa = 11.84GSDD112 pKa = 2.86ATYY115 pKa = 10.44QDD117 pKa = 3.55VLFGLRR123 pKa = 11.84ASEE126 pKa = 4.32SVPSVFSLGHH136 pKa = 5.19PNIYY140 pKa = 10.38KK141 pKa = 10.07DD142 pKa = 4.25DD143 pKa = 4.39KK144 pKa = 11.39NSFWDD149 pKa = 3.91ALASQNITKK158 pKa = 10.23SYY160 pKa = 10.92SIGFADD166 pKa = 4.91APEE169 pKa = 4.68DD170 pKa = 3.75APGVYY175 pKa = 9.89SAEE178 pKa = 4.25DD179 pKa = 3.25AATVSGLIVFGGVAQAYY196 pKa = 8.29YY197 pKa = 10.97NSLTILPLASNDD209 pKa = 3.6DD210 pKa = 4.5LIGFTLTAVGVQADD224 pKa = 4.39TEE226 pKa = 4.51SSPKK230 pKa = 9.37TISAIKK236 pKa = 9.74HH237 pKa = 4.84LAVVKK242 pKa = 9.83TRR244 pKa = 11.84GIFPSMPKK252 pKa = 9.94QLVEE256 pKa = 4.54NIVTSLDD263 pKa = 3.32KK264 pKa = 10.72DD265 pKa = 3.92TEE267 pKa = 4.43LTTGTSGLYY276 pKa = 10.0EE277 pKa = 4.13VDD279 pKa = 3.89CDD281 pKa = 4.48TEE283 pKa = 4.36FTLLFNFQGAVIRR296 pKa = 11.84IPSTEE301 pKa = 4.62LLTEE305 pKa = 4.33SDD307 pKa = 5.04DD308 pKa = 4.4GSCSLAIIPSDD319 pKa = 3.19SDD321 pKa = 3.41EE322 pKa = 4.12FVIAGGMLTYY332 pKa = 9.8FYY334 pKa = 10.88FVVDD338 pKa = 4.44FEE340 pKa = 4.87NDD342 pKa = 3.28QISIGQSKK350 pKa = 9.92FPGANVDD357 pKa = 4.18PEE359 pKa = 4.05IEE361 pKa = 4.39TYY363 pKa = 10.33SGSVSGAKK371 pKa = 8.28TAAYY375 pKa = 9.97YY376 pKa = 10.78SEE378 pKa = 4.54TFSSQLPEE386 pKa = 4.04PTSDD390 pKa = 3.6SSLTSEE396 pKa = 4.2ATTDD400 pKa = 3.34SSTHH404 pKa = 6.75DD405 pKa = 3.81SSSADD410 pKa = 3.3QTVTPSSSEE419 pKa = 3.82SSLTITGYY427 pKa = 8.36NTYY430 pKa = 9.85PYY432 pKa = 9.4TNTSSSSSSSSSSSLASSTSTSTSTVEE459 pKa = 3.82APTISGSCINGQLQWTVNIPASIGPFNAFSYY490 pKa = 10.25EE491 pKa = 4.19GTGEE495 pKa = 4.61GYY497 pKa = 9.23TITNVKK503 pKa = 10.42LNGRR507 pKa = 11.84DD508 pKa = 3.45LTSEE512 pKa = 3.98SGINGAGFSLDD523 pKa = 3.27TTDD526 pKa = 2.86INGRR530 pKa = 11.84SEE532 pKa = 4.45SLTLVYY538 pKa = 9.43TAIRR542 pKa = 11.84FDD544 pKa = 3.68STTLFSSDD552 pKa = 2.53GTLAITRR559 pKa = 11.84PNRR562 pKa = 11.84KK563 pKa = 9.02RR564 pKa = 11.84EE565 pKa = 3.8VLIYY569 pKa = 9.58EE570 pKa = 4.31LSYY573 pKa = 11.02TIDD576 pKa = 3.22TALGYY581 pKa = 10.75AVTNSDD587 pKa = 3.57VEE589 pKa = 4.55EE590 pKa = 4.25TSSVATSTDD599 pKa = 3.19VTSVLSTTVVTATSCSMGICIEE621 pKa = 4.14APITTGVTVATSTVHH636 pKa = 5.05GTVTSFTTYY645 pKa = 10.45CPLSTEE651 pKa = 4.33SFTNSTTPIVTPSTVSLSSTFLVTSPFSPSTEE683 pKa = 3.92VGTTSTPVINPQLEE697 pKa = 4.68VQSSPALSSSSITAPSILTYY717 pKa = 10.68SGGAVGSFTSITNLLFVFPLLLLII741 pKa = 5.19

Molecular weight: 77.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061B0L3|A0A061B0L3_CYBFA Nicotinamide-nucleotide adenylyltransferase OS=Cyberlindnera fabianii OX=36022 GN=BON22_3066 PE=4 SV=1
MM1 pKa = 6.68VKK3 pKa = 10.16EE4 pKa = 4.38RR5 pKa = 11.84IEE7 pKa = 4.17VMSRR11 pKa = 11.84LYY13 pKa = 11.0FNFEE17 pKa = 4.0LFRR20 pKa = 11.84ALTFATGKK28 pKa = 10.42RR29 pKa = 11.84IALPTRR35 pKa = 11.84VEE37 pKa = 4.05PKK39 pKa = 10.45VFFANEE45 pKa = 3.78RR46 pKa = 11.84TFLSWLNFTVVLGGLGVGLLNFGDD70 pKa = 3.55KK71 pKa = 10.34VGRR74 pKa = 11.84VSAGLFTFVAMATMVYY90 pKa = 11.12ALITYY95 pKa = 8.06HH96 pKa = 5.58WRR98 pKa = 11.84AVAIRR103 pKa = 11.84KK104 pKa = 9.53RR105 pKa = 11.84GSGPYY110 pKa = 9.62DD111 pKa = 3.48DD112 pKa = 5.8RR113 pKa = 11.84FGPTMLCLFLLVAVVVNFVLRR134 pKa = 11.84IRR136 pKa = 11.84AAA138 pKa = 3.32

Molecular weight: 15.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5507

0

5507

2608749

49

4725

473.7

53.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.659 ± 0.027

1.031 ± 0.01

6.137 ± 0.026

6.67 ± 0.037

4.299 ± 0.023

5.556 ± 0.029

2.202 ± 0.013

5.965 ± 0.023

6.778 ± 0.035

9.388 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.012

4.608 ± 0.02

4.678 ± 0.029

3.968 ± 0.028

4.513 ± 0.022

8.548 ± 0.048

6.325 ± 0.028

6.323 ± 0.024

1.075 ± 0.011

3.203 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski