Actinomadura rubrisoli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5BR54|A0A4R5BR54_9ACTN Uncharacterized protein OS=Actinomadura rubrisoli OX=2530368 GN=E1298_15510 PE=4 SV=1
MM1 pKa = 7.72YY2 pKa = 10.03EE3 pKa = 3.92DD4 pKa = 5.16GYY6 pKa = 11.58YY7 pKa = 9.49PDD9 pKa = 5.05HH10 pKa = 7.77VVDD13 pKa = 3.62QAKK16 pKa = 10.7AILLRR21 pKa = 11.84LCARR25 pKa = 11.84IEE27 pKa = 4.19AEE29 pKa = 4.76CPADD33 pKa = 4.63LPALYY38 pKa = 10.49VLTHH42 pKa = 7.09AATQEE47 pKa = 4.01FNEE50 pKa = 5.0LEE52 pKa = 4.39DD53 pKa = 5.11DD54 pKa = 3.79FDD56 pKa = 4.6AAGSEE61 pKa = 4.48LEE63 pKa = 4.13TAARR67 pKa = 11.84EE68 pKa = 4.4LIGEE72 pKa = 4.47DD73 pKa = 3.19FWFIATAYY81 pKa = 9.98GFTDD85 pKa = 3.59ADD87 pKa = 3.86HH88 pKa = 7.24EE89 pKa = 4.26EE90 pKa = 4.73LIANRR95 pKa = 11.84DD96 pKa = 3.11WW97 pKa = 4.91

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5BE68|A0A4R5BE68_9ACTN DUF3883 domain-containing protein OS=Actinomadura rubrisoli OX=2530368 GN=E1298_19380 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSGRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.48GRR40 pKa = 11.84SRR42 pKa = 11.84IAVV45 pKa = 3.34

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9016

0

9016

2861743

25

6638

317.4

34.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.617 ± 0.04

0.826 ± 0.007

6.071 ± 0.022

5.575 ± 0.025

2.741 ± 0.015

9.661 ± 0.026

2.257 ± 0.012

3.413 ± 0.015

2.007 ± 0.019

10.316 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.82 ± 0.01

1.696 ± 0.013

6.285 ± 0.024

2.578 ± 0.015

8.781 ± 0.027

4.848 ± 0.019

5.642 ± 0.021

8.341 ± 0.022

1.529 ± 0.011

1.996 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski