Mycobacterium helveticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A557XDJ3|A0A557XDJ3_9MYCO Uncharacterized protein OS=Mycobacterium helveticum OX=2592811 GN=FPZ47_23245 PE=4 SV=1
MM1 pKa = 7.75GFRR4 pKa = 11.84ALPPEE9 pKa = 4.34INSVLMYY16 pKa = 10.24SGAGSTPMLAAAAAWHH32 pKa = 5.62GLGRR36 pKa = 11.84EE37 pKa = 4.28LGSAADD43 pKa = 3.69SFAMVTAGLVGQAWQGPSSAAMLAAAAPYY72 pKa = 10.46VGVLNVAAGQAHH84 pKa = 6.48EE85 pKa = 4.22MAAGAEE91 pKa = 4.22AVASAFEE98 pKa = 4.14AAHH101 pKa = 6.66AATVHH106 pKa = 6.04PSVVAANRR114 pKa = 11.84DD115 pKa = 3.49EE116 pKa = 4.68FVQLVQSNLLGQNAPAIAAAEE137 pKa = 3.85ARR139 pKa = 11.84YY140 pKa = 8.64EE141 pKa = 4.12QMWAADD147 pKa = 3.59VAAMVGYY154 pKa = 10.36HH155 pKa = 5.66GGVWAAAAQLPSWQEE170 pKa = 3.84TLQGLPGLSQVATAIAARR188 pKa = 11.84PVGPAAVVRR197 pKa = 11.84SAASGLAGLPHH208 pKa = 7.39LNTGLGNVGNWNVGGGNVGNTNLGSGNVGNTNLGSGNVGNTNLGSGNVGNTNLGSGNVGNLNLGGGNQGSNNVGSGNSGSGNFGSGNTGNFDD300 pKa = 3.4VGFGNFGSNDD310 pKa = 3.14FGLGNNGNGDD320 pKa = 3.31IGLGLTGNDD329 pKa = 2.82QVGINFAGLMNSGPGNFGLGNSGSDD354 pKa = 3.14NIGFFNSGNGNFGIFNSGDD373 pKa = 3.39GNFGFGNSGDD383 pKa = 3.98TNTGFWNGGSFNSGFGNAADD403 pKa = 4.23LNFGFGNAGAGNMGFWNAGSGNTGFGDD430 pKa = 3.44AGLFNAGNFDD440 pKa = 3.77SGGGTFDD447 pKa = 3.65YY448 pKa = 11.13AGGATVPPGVGSNTGSFNSGNVDD471 pKa = 3.3TGWFNSGDD479 pKa = 3.67SSTGLLNSGTLNTGVGGSANLTGVVASSGYY509 pKa = 9.82GNSGTGSSGFFNSGNSSSGLLNNGSQSAGLFNTGDD544 pKa = 3.62VQSGIFNTGGSNSGFFNRR562 pKa = 11.84DD563 pKa = 2.72YY564 pKa = 11.62DD565 pKa = 3.8NVGIGNTGSDD575 pKa = 3.3NSGLGLSGRR584 pKa = 11.84DD585 pKa = 3.34NTGLSSSGTYY595 pKa = 10.11DD596 pKa = 4.15AGALNQGNYY605 pKa = 7.3QVGILGPGPIATEE618 pKa = 3.99TGTNN622 pKa = 3.36

Molecular weight: 60.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A557XPX4|A0A557XPX4_9MYCO Exodeoxyribonuclease 7 small subunit OS=Mycobacterium helveticum OX=2592811 GN=xseB PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84GIVANRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.24GRR42 pKa = 11.84RR43 pKa = 11.84AISAA47 pKa = 3.59

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5147

0

5147

1625944

21

3709

315.9

33.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.44 ± 0.046

0.889 ± 0.01

6.14 ± 0.027

5.066 ± 0.033

3.007 ± 0.022

9.152 ± 0.043

2.315 ± 0.015

3.982 ± 0.026

2.063 ± 0.023

9.845 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.012 ± 0.015

2.181 ± 0.022

6.014 ± 0.032

2.924 ± 0.018

7.747 ± 0.037

5.207 ± 0.022

5.79 ± 0.022

8.582 ± 0.035

1.503 ± 0.015

2.141 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski