Selaginella moellendorffii (Spikemoss)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Lycopodiopsida; Selaginellales; Selaginellaceae; Selaginella

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33150 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8SB95|D8SB95_SELML TPR_REGION domain-containing protein OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_420200 PE=4 SV=1
MM1 pKa = 7.18LVSVEE6 pKa = 4.29SQSQSEE12 pKa = 4.4SATCKK17 pKa = 8.28EE18 pKa = 3.99QEE20 pKa = 4.09MLLLVNFKK28 pKa = 10.99AGFTDD33 pKa = 3.59SQNMLVHH40 pKa = 6.85WNQNNTNCCKK50 pKa = 10.33WNGITCDD57 pKa = 3.87SLQEE61 pKa = 4.17MIITTAPYY69 pKa = 10.27INGPLPSEE77 pKa = 4.04LAGLTTLQTLIITGTTVWGSIPSEE101 pKa = 4.16LGNLPQLRR109 pKa = 11.84VLDD112 pKa = 4.61LSSNMLSGSIPRR124 pKa = 11.84NLGRR128 pKa = 11.84LQTLRR133 pKa = 11.84EE134 pKa = 4.04LQLASNNLSGSIPWEE149 pKa = 3.98LGSIRR154 pKa = 11.84RR155 pKa = 11.84AYY157 pKa = 10.27LVNLSNNSLSGQIPDD172 pKa = 3.71SLANIAPSGSIDD184 pKa = 3.63LSNNLFTGRR193 pKa = 11.84FPTALCRR200 pKa = 11.84LEE202 pKa = 4.09NRR204 pKa = 11.84SFLFYY209 pKa = 11.1LDD211 pKa = 4.32LSEE214 pKa = 4.57NQLSGALPASLPTTTGSHH232 pKa = 5.08EE233 pKa = 4.37VYY235 pKa = 10.37SYY237 pKa = 11.89LSFLSLASNSLTGTIPSALWSNLSSLTAVDD267 pKa = 4.64FSNNHH272 pKa = 5.72FSGEE276 pKa = 4.15IPTEE280 pKa = 3.76LAGLVGLTSLNLSRR294 pKa = 11.84NDD296 pKa = 3.49LSGEE300 pKa = 3.86IPTSISNGNALQLIDD315 pKa = 5.08LSRR318 pKa = 11.84NTLNGTIPPEE328 pKa = 5.47IGDD331 pKa = 3.98LYY333 pKa = 9.68MLEE336 pKa = 4.29MLDD339 pKa = 4.26LSYY342 pKa = 11.37NQLSGSIPTALDD354 pKa = 3.73DD355 pKa = 4.87LLSLAAFNEE364 pKa = 4.16IYY366 pKa = 10.48LYY368 pKa = 11.25SNNLNGSIPDD378 pKa = 4.7AIANLTRR385 pKa = 11.84LATLDD390 pKa = 3.83LSSNHH395 pKa = 6.99LDD397 pKa = 3.7GQIPGPAIAQLTGLQVMDD415 pKa = 5.2LSANDD420 pKa = 3.53LTGNIPSEE428 pKa = 4.0LADD431 pKa = 4.22LGQLATLDD439 pKa = 4.21LSWNQLSGVIPPEE452 pKa = 3.65IHH454 pKa = 7.13DD455 pKa = 4.61LSSLEE460 pKa = 4.07YY461 pKa = 10.58FSVANNNLSGPIPAEE476 pKa = 4.04LGSFDD481 pKa = 4.35ASSFEE486 pKa = 5.26DD487 pKa = 3.54NAGLCGFPLDD497 pKa = 4.33PCSSS501 pKa = 3.51

Molecular weight: 53.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8S3Z4|D8S3Z4_SELML Uncharacterized protein OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_108395 PE=3 SV=1
MM1 pKa = 7.87PSHH4 pKa = 5.64KK5 pKa = 9.39TFRR8 pKa = 11.84IKK10 pKa = 10.58QKK12 pKa = 10.3LGKK15 pKa = 9.75KK16 pKa = 9.39LRR18 pKa = 11.84QNRR21 pKa = 11.84PIPHH25 pKa = 7.75WIRR28 pKa = 11.84MRR30 pKa = 11.84TNNTIRR36 pKa = 11.84YY37 pKa = 5.73NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33119

31

33150

13328660

30

8462

402.1

44.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.417 ± 0.013

1.905 ± 0.007

5.273 ± 0.008

6.429 ± 0.018

4.096 ± 0.009

6.847 ± 0.014

2.402 ± 0.006

4.705 ± 0.01

5.351 ± 0.013

10.132 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.006

3.437 ± 0.006

4.826 ± 0.013

3.817 ± 0.009

6.002 ± 0.011

8.13 ± 0.014

4.775 ± 0.008

6.977 ± 0.012

1.454 ± 0.005

2.669 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski